Serum-free medium for monkey embryonic stem cell

Abstract

The invention discloses a serum-free medium for a monkey embryonic stem cell. The serum-free medium comprises a basic medium and additives, wherein the additives are dissolved in the basic medium; the basic medium comprises a DMEM-F12 medium; and the additive comprises L-glutamine, non-essential amino acid, L-ascorbic acid, sodium selenite, beta-mercaptoethanol, human serum albumin, heparin sodium, human transferring, insulin human, a basic fibroblast growth factor, a transforming growth factor beta 1 and an epidermal growth factor. The serum-free medium is capable of maintaining an undifferentiation state and totipotency of the monkey embryonic stem cell within a relatively long time, and is applicable to culture of the monkey embryonic stem cell in both a free-feeding layer system and a feeding layer system.

Description

technical field

[0001] The invention relates to the field of stem cell culture, in particular to a serum-free medium for monkey embryonic stem cells. Background technique

[0002] Embryonic stem cells (ESCs) are highly undifferentiated cells isolated from the inner cell mass (Inner Cell Mass, ICM) or primordial germ cells (PGCs) of early embryos and can be cultured in vitro. , which can differentiate into permanent cell lines of various tissues and cell types derived from the 3 germ layers. At present, it is generally believed that embryonic stem cells will have an important impact on the fields of in vitro research on animal and human embryogenesis and development, new gene discovery, and drug screening.

[0003] Although human embryonic stem cells (hESC) have important research and application value in the fields of regenerative medicine, drug screening and developmental biology, the use of human embryonic stem cells and primoryos mainly involves moral and ethical issues....

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