The invention relates to a separation culture method for chick germinal crescent source PGCs. The method comprises the following steps that firstly, according to a separation method, a clean chick embryo without yolk contamination is extracted from a hatching egg through a filter film pasting method, and then germinal crescent tissue is obtained from the chick embryo; secondly, the germinal crescent tissue is digested into single cells through pancreatin, and purification is carried out on the PGCs in the single cells; thirdly, the purified PGCs are placed in a culture system including a BRL-3A cell feed layer and a PGCs complete culture solution to be cultured. The PGCs come from the chick embryo germinal crescent zone, the PGCs content of the 5-8 HH period chick embryo germinal crescent zone is high, better proliferation vitality is achieved, and high-level pluripotency is maintained. The chick embryo germinal disc is separated through the filter film pasting method, operation is easy, repeatability is good, the clean chick embryo without yolk contamination is separated from the hatching egg, and the purified PGCs are obtained. The PGCs cultured through separation can be proliferated to the maximum for later experiment operation.