A serum-free, feeder-free pluripotent stem cell culture method

A technology of pluripotent stem cells and pluripotent stem cells, applied in the field of serum-free and feeder-free pluripotent stem cell culture, can solve the problem of contamination of pluripotent stem cell applications, the inability to guarantee cell biological safety and quality control, and increase the complexity of experiments And other issues
CN103555661BActive Publication Date: 2015-07-29INST OF ZOOLOGY CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF ZOOLOGY CHINESE ACAD OF SCI
Publication Date
2015-07-29

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Abstract

The invention relates to a novel method for culturing a multipotential stem cell without serum and a feeder layer. The method is stable, reliable, and can culture the multipotential stem cell for a long time and also can maintain the self-renewing of the multipotential stem cell. The method is characterized in that a culture system with clear chemical components and without serum and the feeder layer is adopted to perform suspension culture on the multipotential stem cell. According to the morphology of cultured stem cells after 15 generations, mRNA (Messenger Ribonucleic Acid) and protein expression of multipotential marker molecule, and analysis on cell chromatin karyotype, such novel generation system can maintain the properties of the multipotential stem cell during long-term culture. In addition, the differentiative potential of the multipotentail stem cell is further verified by 15-generation suspension culture in an in-vitro embryoid body formation experiment and an in-vivo teratoma formation experiment.
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Description

technical field

[0001] The invention relates to a system and method for culturing pluripotent stem cells in suspension without serum and feeder layer. Specifically, the present invention relates to a system and method for suspension culture of pluripotent stem cells using a serum-free, feeder-free, and chemically defined culture system. Background technique

[0002] In recent decades, the study of stem cell biology has advanced people's understanding of many basic biological issues, and has also prompted people to explore cell therapy methods for many diseases. Since Evans and Matthew Kaufman first discovered mouse embryonic stem cells in 1981, embryonic stem cell research has been the focus of attention. Since then, the acquisition of induced pluripotent stem cells (iPSCs) has brought pluripotent stem cell research to a climax. Mouse pluripotent stem cells (including mouse embryonic stem cells and iPSCs) are often used as the best cell model for studying the growth, diffe...

Claims

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