79 results about "Cell Pellet" patented technology

The present invention relates to the technical field of cell detection, and specifically to a finished product detection device and method for stem cell drugs, including a centrifuge body and a cover body hinged to the top thereof. Inside the top of the centrifuge body, there is a tray and a sampling part. The sampling part is placed inside the center of the tray and rotates around this central axis; a motor is provided at the center of the bottom of the tray, and flexible heating sheets are symmetrically arranged on the bottom side wall of the tray; the sampling part includes a number of sampling tubes, a number of sampling sleeves correspondingly sleeved with the sampling tubes, and a centrifuge cylinder coaxially connected to the motor. Through the linkage design of the pressing pad on the cover body and the sampling sleeve pressing block in the present invention, when the cover body is closed, the sampling tube is automatically triggered to incline towards the center, and the aggregation efficiency of cell precipitation after centrifugation is improved; after the cover is opened, the folding elastic piece resets the sampling tube to an upright state, which is convenient for accurately and quickly sucking out the supernatant and reducing the cell exposure time.

The present application relates to a centrifugal system, a cell processing method and an apparatus. The centrifugal system includes: a sample chamber, a mixing and resuspension chamber, a collection chamber and a waste liquid chamber; the sample chamber is used to place a preset reagent; the mixing and resuspension chamber is connected to the sample chamber and has a first channel that allows the liquid in the sample chamber to enter the mixing and resuspension chamber at a preset centrifugal speed, and is used to mix the preset reagent entering from the first channel and the sample liquid to be tested input from the input channel at the mixing centrifugal speed to obtain a mixed liquid, and separate the mixed liquid to obtain a cell precipitate and a waste liquid; the collection chamber is used to collect the cell precipitate; the waste liquid chamber is used to collect the waste liquid. The centrifugal structure can realize automated centrifugal separation while reducing damage to cell morphology and interference with other cells.

The invention belongs to the technical field of exosomes, and discloses an exosome preparation method and application, the exosome preparation method comprises the following steps: S1, culturing target cells in an induction medium, and collecting the cells and a culture solution; s2, separating the culture solution to obtain supernate A and cell precipitate; s3, performing chromatographic purification and concentration on the supernate A to obtain the exosome; in the step S1, colchicine is also added in the process of culturing the target cells to synchronize the cell cycle; a polycaprolactone-gelatin composite microcarrier is suspended in the induction culture medium, and nicotinamide, an epidermal growth factor (EGF) and pyruvic acid are contained in the induction culture medium; the method further comprises a step S30 before the step S3, resuspending the cell precipitate obtained in the step S2 with a vesicle protection solution containing trehalose, sucrose and EDTA to obtain a supernatant B, and then combining the supernatant A with the supernatant B. According to the preparation method provided by the invention, the total secretion amount of target cells is large, the intracellular vesicle-exosome precursor can be collected, and the obtained exosome is high in concentration and low in impurity content.

A battery assembly includes: a first cell block; a first cover coupled to a first side of the first cell block; and a second cover coupled to a second side of the first cell block, the second side being opposite the first side. The first cover includes a first extension portion extending from the first cover. The first extended portion includes a first plate spaced apart from the surface of the first cover in a first direction. The first extension portion further includes a second plate between the first plate and a first surface of the first cover.

The present invention relates to the technical field of cell culture, and specifically to a cell culture device for immune cell amplification, which includes an incubator, a culture plate disposed inside the incubator, a culture bottle disposed above the culture plate, a bottle-turning mechanism fixedly disposed on the upper surface of the culture plate and driving the culture bottle to rotate by 90 degrees, a medicine-adding mechanism for injecting nutrient solution into the culture bottle, a liquid-sucking mechanism for sucking out the nutrient solution from the culture bottle, a substrate fixedly disposed on the rear inner wall of the incubator and located below the culture plate, and an active precipitation mechanism fixedly disposed on the substrate and alternately driving the culture plate to perform upward acceleration movement and downward uniform movement. The present invention adopts the active precipitation mechanism to make the culture bottle in a superheavy state, accelerating the precipitation of cells in the culture bottle. The medicine-adding mechanism can automatically supplement the nutrient solution into the culture bottle, and the liquid-sucking mechanism can suck out part of the old nutrient solution in the culture bottle, facilitating semi-quantity replacement of the cells.

The invention relates to a method of producing an ester of a fatty acid and a C1-C4 alcohol, comprising successive steps of: cultivating cells of a microalga strain on a growth medium, harvesting the cultivated cells and concentrating them to obtain a wet cell pellet, suspending said wet cell pellet in an hydroalcoholic solution comprising said C1-C4 alcohol, incubating the cell suspension thus formed so as to form said ester of a fatty acid and a C1-C4 alcohol by transesterification and / or esterification of lipids produced by the microalga cells catalyzed by endogenous enzymes of said cells, and recovering said ester of a fatty acid and a C1-C4 alcohol from said cell suspension. The growth medium can advantageously be a dark fermentation effluent.

The invention relates to an isolated culture method of adipose-derived stem cells. The isolated culture method comprises the following steps: S1, aseptically acquiring adipose tissues of a human body; s2, cutting the obtained adipose tissue into small blocks of 1-2mm < 3 >, putting the small blocks into a sterile culture bottle for culture, and digesting to obtain a uniform suspension; s3, filtering the digested suspension through a cell filter, carrying out centrifugal treatment on the filtered liquid, and removing supernate to obtain cell precipitate; s4, after the cell precipitates are resuspended, a culture dish is inoculated with the cell precipitates, the cell precipitates are put into an incubator to be cultured, a culture medium is replaced regularly, the culture medium contains acetic acid, and after passage operation, adipose-derived stem cells obtained after passage culture are obtained; and S5, detecting and cryopreserving. According to the application, the stress resistance of the adipose-derived stem cells is improved through pretreatment by utilizing the protection effect of acetic acid under a cell stress condition, and the adaptability of the adipose-derived stem cells is improved by simulating an in-vivo survival microenvironment through a pretreatment strategy, so that the survival rate of the adipose-derived stem cells in the survival microenvironment after transplantation is improved, and the survival rate of the adipose-derived stem cells is improved. The application potential in tissue repair and regeneration treatment is enhanced.

The invention discloses an embryo dynamic 3D suspension culture device which comprises a base, a plurality of placing grooves are uniformly formed in the upper end of the base, culture plates are placed in the placing grooves, a plurality of culture grooves are uniformly formed in the culture plates, stirring assemblies used in cooperation with the culture plates are arranged in the placing grooves, and sealing plates are arranged at the upper ends of the culture grooves. A water inlet pipeline, a water outlet pipeline, an air inlet pipeline and an air outlet pipeline are arranged on the sealing plate, and a water inlet assembly, a water outlet assembly, an air inlet assembly and an air outlet assembly are arranged in the base. A culture solution is uniformly stirred through the stirring assembly, uniform suspension of cells or organoid in the culture solution is promoted, cell precipitation is prevented, blood flow or body fluid flow in a living body is simulated by entering and exiting the culture solution at a constant speed and controlling shearing force generated by liquid flow, and therefore a dynamic 3D environment is provided; and culture conditions closer to reality are provided.

The utility model discloses a low disturbance cell collection system, including the outer tube, the inner tube is arranged in the outer tube, the inner tube is covered and has the pipe cover, wherein, the bottom of inner tube is provided with the sedimentation cavity for the cell precipitation, and the sidewall of inner tube and located above the sedimentation cavity is provided with a plurality of drainage groove, and the baffle is inserted in the drainage groove. The utility model discloses a baffle is inserted in the drainage groove sealing, and the inner tube is placed in the outer tube and pours into the centrifugal liquid and covers the pipe cover, and then is placed into the centrifugal equipment and is centrifuged. After centrifugation, the liquid is layered, and the cell is precipitated in the sedimentation cavity, and the upper layer is liquid. At this time, only need to open the pipe cover, and the baffle is pulled up, and the bottom of drainage groove is opened, and the upper layer liquid will flow into the outer tube naturally, and the cell is steady and stays in the sedimentation cavity, and solid-liquid separation is realized easily. The whole process is simple to operate, and the complicated transfer or filtering step is not needed, so that the disturbance of human operation to the cell is reduced greatly, and the integrity and activity of the cell are protected to the maximum extent.

In an aspect, the present disclosure provides a method for determining a cancer assessment of a subject, comprising: (a) obtaining a whole blood sample from the subject; (b) performing centrifugation on the whole blood sample to obtain a plasma layer, a serum layer, a supernatant layer, or a cell pellet; (c) assaying at least a portion of the plasma layer, the serum layer, the supernatant layer, or the cell pellet, or derivatives thereof, to generate a Raman spectroscopy profile of the whole blood sample of the subject; (d) processing the Raman spectroscopy profile of the whole blood sample of the subject using a trained machine learning algorithm or against a reference; and (e) determining the cancer assessment of the subject, based at least in part on the processing in (d).

The invention discloses a preparation method of uterine blood mesenchymal stem cells, and relates to the technical field of uterine blood mesenchymal stem cell preparation, and the preparation method comprises the following steps: filtering and diluting a uterine blood sample, adding the diluted uterine blood sample into a sample density separation medium, centrifuging, collecting an upper layer and a middle layer, repeatedly centrifuging, and taking cell precipitate to obtain primary cells of the uterine blood mesenchymal stem cells; resuspending, culturing until the fusion degree of the cells reaches 70-90%, digesting by using a recombinant trypsin solution, collecting a cell suspension, centrifuging, resuspending, inoculating and culturing until the fusion degree reaches 70-90%, digesting by using a recombinant trypsin solution, centrifuging, and resuspending; centrifuging for two times, taking cell precipitate, and resuspending by using a cell freezing medium to obtain the uterine blood mesenchymal stem cells. According to the method, after the uterine blood sample is filtered, the centrifugation step is omitted, and the uterine blood sample is directly added to the upper layer of the sample density separation liquid for centrifugal extraction, so that the normal living cell proportion of primary cells and the adherence rate after inoculation are obviously improved, the production cycle is obviously shortened, and the yield of the primary cells is improved.

The application discloses a method for separating and culturing primary vascular smooth muscle cells from human abdominal aortic wall, and relates to the technical field of human cell culture. The method comprises the following steps: obtaining a collagenase A solution; adding the collagenase A solution to a treated human abdominal aortic aneurysm wall tissue sample and then cutting the sample; transferring the cut human abdominal aortic aneurysm wall tissue sample to a 37-degree cell culture box for culture; centrifuging a cell suspension obtained after termination of digestion; discarding supernatant, resuspending cell precipitate, and repeating centrifugation for three times; resuspending the cell precipitate again, uniformly transferring the cell precipitate to a T25 culture bottle after blowing, and placing the cell precipitate in a 37-degree cell culture box for culture; when a wave peak and wave trough state appears, the cell precipitate is digested and subcultured by using 0.25% trypsin, and then the cell precipitate is transferred to a new T25 culture bottle; and the cell precipitate is subcultured normally to the P3 generation and then stored in liquid nitrogen, so that the cell damage is prevented and the activity and proliferation capacity of the cell are maintained.

A method for analyzing a tumor-specific marker and to the use of an isolate from ejaculate for diagnosing and / or monitoring prostate cancer, and / or for monitoring the success of prostate cancer therapy. The method includes the steps of performing an analysis on the isolate, wherein the tumor-specific marker selected from a protein, DNA, and RNA is analyzed, wherein the isolate was obtained by fractionating ejaculate into a low-density cell population and a high-density cell population, wherein the high-density cell population including mature sperm cells was separated and the isolate includes the low-density cell population, wherein the isolate includes a cell pellet.

The invention discloses a construction method of a tumor organoid microenvironment chip model, the model and application of the model. The construction method comprises the following steps: acquiring a tumor organ and preparing cell precipitate; carrying out enzymolysis and digestion treatment on the cell precipitate, and then adding an extracellular matrix to prepare a tumor organ suspension; the method comprises the following steps: adding a tumor organoid suspension into a micro-fluidic culture carrier, culturing to prepare suspensions of fibroblasts and vascular endothelial cells, respectively adding the two cell suspensions into corresponding hole sites of the micro-fluidic culture carrier, and finally culturing to obtain the tumor organoid microenvironment chip model. The model prepared by the method can more truly simulate the complex tumor microenvironment in vivo, is suitable for drug screening and drug sensitivity detection, is beneficial to improving the accuracy and reliability of research, and accelerates the transformation from laboratory research to clinical application.

The invention discloses a method for extracting high-purity liver cell membranes of pelteobagrus fulvidraco. The method comprises the following steps: firstly, cutting the liver into pieces to obtain large liver tissues; digesting the large liver tissue for multiple times by using isopyknic protease liquid; adding an extraction reagent 1 containing a protease inhibitor and a phosphatase inhibitor into the digested small tissues and cell precipitates, and ultrasonically crushing to obtain homogenate; performing low-speed centrifugation on the homogenate for multiple times to collect supernate, and performing high-speed centrifugation for multiple times to collect clear liquid; carrying out ultracentrifugation to collect the precipitate, and resuspending; obtaining a coarse cell membrane suspension; adding the coarse cell membrane suspension into a centrifugal tube, then adding a sucrose density gradient buffer solution until the centrifugal tube is filled with the coarse cell membrane suspension, and carrying out ultracentrifugation; and resuspending the cell membrane precipitate to obtain the pelteobagrus fulvidraco liver cell membrane. Immunoblotting verification on the cell membrane and other organelles extracted by the method shows that the cell membrane extracted by the method conforms to the expected effect of high purity, and other organelles contained in the cell membrane have few impurities.

The invention relates to the field of cell culture, in particular to a culture method for efficiently separating autologous skin source fibroblasts, which comprises the following steps: S101, obtaining skin tissue fragments; s102, obtaining a first mixture; s103, obtaining a second mixture; s104, obtaining a third mixture; s105, obtaining a fourth mixture; s106, obtaining a cell precipitate; and S107, performing precipitation and resuspension on the cells by using a fibroblast selective culture medium to obtain a cell suspension, thereby obtaining high-purity fibroblasts. The separation method and the fibroblast selective culture medium provided by the invention can synergistically ensure efficient separation of the fibroblasts and maintenance of high purity and high activity in the culture period, so that the culture period of the fibroblasts is shortened, and the separation method and the fibroblast selective culture medium show a wide application scene in the aspects of beauty, aging resistance, regenerative medicine and the like.

The present invention discloses a method for accelerating cell sedimentation, and belongs to the field of cell classification and counting. The method includes the steps of placing the counting plate in an electric field before moving the counting plate to the observation area for observation, and utilizing the characteristics of the cell membrane being charged to accelerate the cell sedimentation to the bottom of the counting plate. The present invention utilizes the characteristics of the cells being charged and places them in an electric field. Since the charged particles will be accelerated to fall to a pole with different electrical properties in the electric field, the purpose of accelerating cell sedimentation is achieved. The present invention also discloses a metal plate matching the above method and a microscope containing the counting plate. The metal plate has a simple structure and is easy to manufacture. There are multiple ways to charge the metal plate, which are easy to implement. Integrating the metal plate into the microscope can significantly improve the efficiency of the microscope in processing cell detection work.

This invention relates to the field of biomedical technology and provides a controllable cryogenic treatment mesenchymal stem cell preparation, its preparation method, and its application. The method includes the following steps: cell preparation and cryopreservation equilibration: digestion and collection; cryopreservation equilibration; removal of cryoprotectant and washing: centrifugation to remove cryopreservation solution; PBS washing; cryogenic incubation: resuspending the final cell pellet after multiple washes in pre-cooled PBS; incubating the cell suspension at 4±0.5℃ for 72 hours±10 minutes; after incubation, removing the cell suspension from the 4℃ environment, centrifuging, and resuspending in PBS. This invention yields a ready-to-use, easily stored and transported, and functionally enhanced RT-MSCs preparation. Although the cells in this preparation have lost their proliferative and metabolic activity, they still retain the complete therapeutic functions of MSCs, such as immunomodulation, anti-fibrosis, and angiogenesis promotion.

The invention provides a lung qi and blood barrier protection effect evaluation method. Specifically, the invention provides a method for evaluating the lung qi and blood barrier protection effect of a to-be-detected medicine, which comprises the following steps: (i) providing a pulmonary alveolar lavage fluid (BALF) sample of a subject receiving the to-be-detected medicine and / or reference substance administration; (ii) extracting a cell precipitate from the BALF and lysing the cell precipitate to obtain a protein extract; (iii) dyeing the protein extract and the protein standard substance with a Prussian blue dyeing solution; (iv) measuring the absorbance at the wavelength of 680 + / -10nm by using a microplate reader; wherein a standard curve is drawn according to the absorbance of the protein standard substances with different concentrations, and the absorbance of the protein extract is substituted into the standard curve to calculate the concentration of the ferrihemicin, so that the protective effect of the medicine on the lung qi and blood barrier is judged. The method disclosed by the invention can be used for simply, quickly and accurately evaluating the lung qi and blood barrier protection effect of the to-be-detected medicine.

The present application belongs to the technical field of quality control materials. Disclosed are a quality control material freeze-dried powder as well as a preparation method therefor and the use thereof. The quality control material freeze-dried powder is obtained by freeze-drying cell pellets containing cell fragments, the cell pellets being obtained by sectioning, deparaffinizing and hydrating a paraffin-embedded cell sample and then centrifuging same. The quality control material freeze-dried powder prepared by using the preparation method in the present application can reduce the loss of cell fragments during the freeze-drying process, so as to ensure the high recovery rate of cells, can avoid performance change or protein loss of cell fragments during the freeze-drying process, and can ensure that the immunohistochemical staining performance of reconstituted cell pellets after freeze-drying remains unchanged. The quality control material freeze-dried powder obtained by using the preparation method in the present application has extended storage time and facilitates transport, and the reconstituted cell suspension has good dispersibility, retains key target substances, and exhibits normal immunohistochemical staining performance.

The invention discloses a construction method of a primary cell organoid model and application of the primary cell organoid model in drug screening, the construction method comprises the following steps: (1) centrifuging malignant pleuroperitoneal fluid samples derived from digestive tract tumor patients, collecting cell precipitates, and preparing a single-cell suspension; (2) performing primary culture on the single-cell suspension to obtain primary culture cells; and (3) mixing the primary culture cells with matrigel, and carrying out organoid culture to obtain the primary cell organoid model. By adopting the method provided by the invention, the homogeneity of the organoids cultured by using the primary cells is higher, the number of successfully cultured organoids is more, and meanwhile, the related problems of limited passage and organoid amplification culture library building in the field of organoid culture are solved.

The utility model relates to the technical field of cell extraction, and provides a centrifugal tube suitable for cell precipitation extraction, which comprises a first centrifugal tube, a second centrifugal tube and a fixing mechanism, the first centrifugal tube is detachably connected with the second centrifugal tube through the fixing mechanism, and scale marks are carved on the outer side walls of the first centrifugal tube and the second centrifugal tube; the fixing mechanism comprises a first connecting block and a second connecting block, the first connecting block is mounted on the first centrifugal tube, the second connecting block is mounted on the second centrifugal tube, and a plurality of limiting pieces are mounted at the top of the second connecting block. The centrifugal tube has the beneficial effects that the centrifugal tube is divided into two parts, namely the upper tube body and the lower tube body, and the two parts are detachably connected by adopting the fixing mechanism, so that the centrifugal tube can be easily assembled and disassembled, and the upper tube body can be slightly rotated to be separated from the lower tube body in cell precipitation extraction work, so that the extraction depth is reduced, and the extraction efficiency is improved. The bottom sediment can be more easily and completely taken out, and the cell sediment extraction work is facilitated.

The application belongs to the technical field of pharmaceutical analysis, and provides a method for quantitatively determining the concentration of PROTAC drugs in cells with high specificity, comprising the following steps: (1) selecting a cell strain expressing a PROTAC drug target; (2) culturing the cell strain to the logarithmic growth phase; (3) inoculating the cell suspension on a cell culture plate for culture; (4) adding the PROTAC drug to the cell culture plate, mixing and continuing to incubate; (5) removing the cell culture medium in the cell culture plate; dispersing the cell culture medium into a single cell suspension, centrifuging to discard the supernatant and obtain a cell precipitate; (6) lysing the cell precipitate, collecting the supernatant by ultracentrifugation, and performing ultra-high performance liquid chromatography-tandem mass spectrometry analysis; (7) preparing a blank cell suspension and a series of standard solutions of PROTAC drug concentrations, performing ultra-high performance liquid chromatography-tandem mass spectrometry analysis, and constructing a standard curve; and (8) using the standard curve to obtain the concentration of the PROTAC drug in the cells.

The present application relates to the technical field of medicine, and more particularly to a boronized cell membrane and a preparation method and application thereof.The preparation method of the boronized cell membrane comprises the following steps: adding tumor cells into a culture medium containing a monosaccharide-modified boron drug to perform incubation, collecting cell precipitates after the incubation is completed, and then extracting cell membranes, so that the boronized cell membrane is obtained.The present application provides a boronized cell membrane and a preparation method and application thereof.By using the monosaccharide-modified BSH, the obtained boron drug is anchored on the tumor cell membrane, and then the boron drug is delivered to the tumor tissue by the homologous targeting property of the cell membrane itself, so that the boronized cell membrane has high biocompatibility and helps to better achieve BNCT treatment.

Methods are provided to separate target cells from non-target cells in a liquid biopsy sample, based on the cells' sedimentation properties. One method involves providing a container comprising a volume Vd of a density medium and then adding a volume Vb of a liquid biopsy sample on top of the density medium; the volume Vb of the liquid biopsy sample is a sum of the volume of a liquid fraction Vp and the volume of cells Vc in the liquid biopsy sample. Next, the non-target cells in the liquid biopsy sample are allowed to sediment in a sedimentation layer at the bottom of the container; the sedimentation layer comprises a liquid volume Vremaining between the sedimented cells. Lastly, the supernatant comprising target cells is collected. In this method, the density of the density medium is higher than the density of the liquid biopsy sample but lower than the density of the non-target cells and the volume Vd of the density medium is at least equal to Vremaining. A method is provided to separate target cells from non-target cells in a liquid biopsy sample, based on the cells' sedimentation properties The method involves first providing a container comprising a volume Vd of a density medium. Next, a volume Vb of a liquid biopsy sample is added on top of the density medium. The non-target cells in the liquid biopsy sample are then allowed to sediment in the density medium. Next, the supernatant comprising target cells is collected. The volume Vb of the liquid biopsy sample is a sum of the volume of a liquid fraction Vp and the volume of cells Vc in the liquid biopsy sample, the volume Vd of the density medium is at least equal to the Vp. A kit to separate target cells from non-target cells in a liquid biopsy sample includes a container that contains a volume Vd of a density medium, and instructions to carry out the method.

The utility model relates to the technical field of separation devices, in particular to a primary cell digestion rapid separation device which comprises a centrifugal box, a first motor is fixedly connected to the lower surface of the inner side wall of the centrifugal box, and a centrifugal groove is fixedly connected to the output shaft end of the first motor. A second motor is started to drive a threaded rod to rotate, drive a nut frame and a toothed plate to move, enable a half gear meshed with the toothed plate to rotate, and drive a rotating rod, a mounting seat and a clamping frame to rotate, so that the inclination angle of a test tube is adjusted, and then a first motor is started to drive a centrifugal groove to rotate quickly; therefore, rapid separation after primary cell digestion is completed, different inclination angles can be set according to requirements, so that ideal cell precipitation liquid is obtained, layering of the cell precipitation liquid better meets the separation requirement, and practicability is higher.

The invention discloses an efficient universal mouse pancreatic tissue single-cell suspension preparation method, and belongs to the technical field of cell biology. Pancreas are rich in digestive enzymes, so that the treatment difficulty of in-vitro cells is high, and a high-quality single-cell suspension is difficult to prepare by a traditional method. The method comprises the steps of reagent preparation, tissue sampling, tissue dissociation, red cracking and cleaning, AOPI dyeing quality inspection and the like. Components and concentrations of a tissue enzymatic hydrolysate, a cell cleaning solution and a cell suspension are determined in reagent preparation; the acquisition method of the pancreatic tissue of the mouse is defined by tissue sampling; the tissue dissociation adopts a two-round digestion mode, and the cut tissue is subjected to enzymolysis by using a compound enzyme solution under specific conditions; the cell precipitates are treated through red cracking and cleaning; aOPI dyeing quality inspection is used for analyzing cell count and motility rate. According to the method, the culture medium containing specific components and the compound enzyme liquid are creatively used, the problem that pancreas single cells are difficult to dissociate can be effectively solved, the single-cell suspension with high motility rate, high purity and low adhesion is obtained, and the requirements of downstream single-cell experiments are met.

The application discloses a quality control product for human papillomavirus molecular detection and a preparation method thereof, and belongs to the technical field of biological products. After synthesizing 25 types of HPV full genomes of high-risk types (HPV16, HPV18, HPV26, HPV31, HPV33, HPV35, HPV39, HPV45, HPV51, HPV52, HPV53, HPV56, HPV58, HPV59, HPV66, HPV68, HPV73, HPV82) and low-risk types (HPV6, HPV11, HPV42, HPV43, HPV44, HPV81, HPV83), the HPV full genomes are connected into a pcDNA3.1(+) skeleton vector system, the HPV full genomes are integrated into the genome of a human ovarian cancer cell line ovcar3 respectively, and 25 strains of human ovarian cancer cell lines stably integrating the HPV full genomes are obtained. The new quality control product or kit of various types of simulated clinical samples such as genomic DNA, cell precipitate and cell suspension can be prepared and is suitable for various detection platforms. The human cell line quality control product stably integrating the full-length genomes of the 25 HPV types can more accurately determine the viral load and control the whole process of HPV detection.

A programmed cooling instrument comprises a cooling box with a cryopreservation slot and further comprises a controller and a pre-cooling pool. An oscillation shaking table is mounted below the cooling box; a liquid inlet of the pre-cooling pool is connected with a liquid outlet of the cooling box through a pipeline, and a liquid outlet of the pre-cooling pool is connected with a liquid inlet of the cooling box through a pipeline; a loop is formed among the precooling pool, the cooling box and the two pipelines; the loop is filled with a liquid refrigerant, and one of the two pipelines is provided with a pump body for pushing the liquid refrigerant to circulate; a refrigeration sheet is mounted on the side edge of the pre-cooling pool; and the controller is respectively connected with the oscillation shaking table, the pump body and the refrigeration sheet through leads. The oscillating shaker is mounted below the cooling box, samples are uniformly mixed by the oscillating shaker module before phase change of cell suspension, cell precipitation can be prevented in the cooling process, sample temperature is homogenized, simultaneous phase change is realized, and freezing survival rate is increased in a time-shortened manner.