41 results about "Cell Pellet" patented technology

The invention belongs to the technical field of exosomes, and discloses an exosome preparation method and application, the exosome preparation method comprises the following steps: S1, culturing target cells in an induction medium, and collecting the cells and a culture solution; s2, separating the culture solution to obtain supernate A and cell precipitate; s3, performing chromatographic purification and concentration on the supernate A to obtain the exosome; in the step S1, colchicine is also added in the process of culturing the target cells to synchronize the cell cycle; a polycaprolactone-gelatin composite microcarrier is suspended in the induction culture medium, and nicotinamide, an epidermal growth factor (EGF) and pyruvic acid are contained in the induction culture medium; the method further comprises a step S30 before the step S3, resuspending the cell precipitate obtained in the step S2 with a vesicle protection solution containing trehalose, sucrose and EDTA to obtain a supernatant B, and then combining the supernatant A with the supernatant B. According to the preparation method provided by the invention, the total secretion amount of target cells is large, the intracellular vesicle-exosome precursor can be collected, and the obtained exosome is high in concentration and low in impurity content.

The invention relates to a method of producing an ester of a fatty acid and a C1-C4 alcohol, comprising successive steps of: cultivating cells of a microalga strain on a growth medium, harvesting the cultivated cells and concentrating them to obtain a wet cell pellet, suspending said wet cell pellet in an hydroalcoholic solution comprising said C1-C4 alcohol, incubating the cell suspension thus formed so as to form said ester of a fatty acid and a C1-C4 alcohol by transesterification and / or esterification of lipids produced by the microalga cells catalyzed by endogenous enzymes of said cells, and recovering said ester of a fatty acid and a C1-C4 alcohol from said cell suspension. The growth medium can advantageously be a dark fermentation effluent.

The utility model discloses a low disturbance cell collection system, including the outer tube, the inner tube is arranged in the outer tube, the inner tube is covered and has the pipe cover, wherein, the bottom of inner tube is provided with the sedimentation cavity for the cell precipitation, and the sidewall of inner tube and located above the sedimentation cavity is provided with a plurality of drainage groove, and the baffle is inserted in the drainage groove. The utility model discloses a baffle is inserted in the drainage groove sealing, and the inner tube is placed in the outer tube and pours into the centrifugal liquid and covers the pipe cover, and then is placed into the centrifugal equipment and is centrifuged. After centrifugation, the liquid is layered, and the cell is precipitated in the sedimentation cavity, and the upper layer is liquid. At this time, only need to open the pipe cover, and the baffle is pulled up, and the bottom of drainage groove is opened, and the upper layer liquid will flow into the outer tube naturally, and the cell is steady and stays in the sedimentation cavity, and solid-liquid separation is realized easily. The whole process is simple to operate, and the complicated transfer or filtering step is not needed, so that the disturbance of human operation to the cell is reduced greatly, and the integrity and activity of the cell are protected to the maximum extent.

The invention discloses a preparation method of uterine blood mesenchymal stem cells, and relates to the technical field of uterine blood mesenchymal stem cell preparation, and the preparation method comprises the following steps: filtering and diluting a uterine blood sample, adding the diluted uterine blood sample into a sample density separation medium, centrifuging, collecting an upper layer and a middle layer, repeatedly centrifuging, and taking cell precipitate to obtain primary cells of the uterine blood mesenchymal stem cells; resuspending, culturing until the fusion degree of the cells reaches 70-90%, digesting by using a recombinant trypsin solution, collecting a cell suspension, centrifuging, resuspending, inoculating and culturing until the fusion degree reaches 70-90%, digesting by using a recombinant trypsin solution, centrifuging, and resuspending; centrifuging for two times, taking cell precipitate, and resuspending by using a cell freezing medium to obtain the uterine blood mesenchymal stem cells. According to the method, after the uterine blood sample is filtered, the centrifugation step is omitted, and the uterine blood sample is directly added to the upper layer of the sample density separation liquid for centrifugal extraction, so that the normal living cell proportion of primary cells and the adherence rate after inoculation are obviously improved, the production cycle is obviously shortened, and the yield of the primary cells is improved.

The application discloses a method for separating and culturing primary vascular smooth muscle cells from human abdominal aortic wall, and relates to the technical field of human cell culture. The method comprises the following steps: obtaining a collagenase A solution; adding the collagenase A solution to a treated human abdominal aortic aneurysm wall tissue sample and then cutting the sample; transferring the cut human abdominal aortic aneurysm wall tissue sample to a 37-degree cell culture box for culture; centrifuging a cell suspension obtained after termination of digestion; discarding supernatant, resuspending cell precipitate, and repeating centrifugation for three times; resuspending the cell precipitate again, uniformly transferring the cell precipitate to a T25 culture bottle after blowing, and placing the cell precipitate in a 37-degree cell culture box for culture; when a wave peak and wave trough state appears, the cell precipitate is digested and subcultured by using 0.25% trypsin, and then the cell precipitate is transferred to a new T25 culture bottle; and the cell precipitate is subcultured normally to the P3 generation and then stored in liquid nitrogen, so that the cell damage is prevented and the activity and proliferation capacity of the cell are maintained.

A method for analyzing a tumor-specific marker and to the use of an isolate from ejaculate for diagnosing and / or monitoring prostate cancer, and / or for monitoring the success of prostate cancer therapy. The method includes the steps of performing an analysis on the isolate, wherein the tumor-specific marker selected from a protein, DNA, and RNA is analyzed, wherein the isolate was obtained by fractionating ejaculate into a low-density cell population and a high-density cell population, wherein the high-density cell population including mature sperm cells was separated and the isolate includes the low-density cell population, wherein the isolate includes a cell pellet.

The invention discloses a construction method of a tumor organoid microenvironment chip model, the model and application of the model. The construction method comprises the following steps: acquiring a tumor organ and preparing cell precipitate; carrying out enzymolysis and digestion treatment on the cell precipitate, and then adding an extracellular matrix to prepare a tumor organ suspension; the method comprises the following steps: adding a tumor organoid suspension into a micro-fluidic culture carrier, culturing to prepare suspensions of fibroblasts and vascular endothelial cells, respectively adding the two cell suspensions into corresponding hole sites of the micro-fluidic culture carrier, and finally culturing to obtain the tumor organoid microenvironment chip model. The model prepared by the method can more truly simulate the complex tumor microenvironment in vivo, is suitable for drug screening and drug sensitivity detection, is beneficial to improving the accuracy and reliability of research, and accelerates the transformation from laboratory research to clinical application.

The invention discloses a method for extracting high-purity liver cell membranes of pelteobagrus fulvidraco. The method comprises the following steps: firstly, cutting the liver into pieces to obtain large liver tissues; digesting the large liver tissue for multiple times by using isopyknic protease liquid; adding an extraction reagent 1 containing a protease inhibitor and a phosphatase inhibitor into the digested small tissues and cell precipitates, and ultrasonically crushing to obtain homogenate; performing low-speed centrifugation on the homogenate for multiple times to collect supernate, and performing high-speed centrifugation for multiple times to collect clear liquid; carrying out ultracentrifugation to collect the precipitate, and resuspending; obtaining a coarse cell membrane suspension; adding the coarse cell membrane suspension into a centrifugal tube, then adding a sucrose density gradient buffer solution until the centrifugal tube is filled with the coarse cell membrane suspension, and carrying out ultracentrifugation; and resuspending the cell membrane precipitate to obtain the pelteobagrus fulvidraco liver cell membrane. Immunoblotting verification on the cell membrane and other organelles extracted by the method shows that the cell membrane extracted by the method conforms to the expected effect of high purity, and other organelles contained in the cell membrane have few impurities.

This invention relates to the field of biomedical technology and provides a controllable cryogenic treatment mesenchymal stem cell preparation, its preparation method, and its application. The method includes the following steps: cell preparation and cryopreservation equilibration: digestion and collection; cryopreservation equilibration; removal of cryoprotectant and washing: centrifugation to remove cryopreservation solution; PBS washing; cryogenic incubation: resuspending the final cell pellet after multiple washes in pre-cooled PBS; incubating the cell suspension at 4±0.5℃ for 72 hours±10 minutes; after incubation, removing the cell suspension from the 4℃ environment, centrifuging, and resuspending in PBS. This invention yields a ready-to-use, easily stored and transported, and functionally enhanced RT-MSCs preparation. Although the cells in this preparation have lost their proliferative and metabolic activity, they still retain the complete therapeutic functions of MSCs, such as immunomodulation, anti-fibrosis, and angiogenesis promotion.

The invention provides a lung qi and blood barrier protection effect evaluation method. Specifically, the invention provides a method for evaluating the lung qi and blood barrier protection effect of a to-be-detected medicine, which comprises the following steps: (i) providing a pulmonary alveolar lavage fluid (BALF) sample of a subject receiving the to-be-detected medicine and / or reference substance administration; (ii) extracting a cell precipitate from the BALF and lysing the cell precipitate to obtain a protein extract; (iii) dyeing the protein extract and the protein standard substance with a Prussian blue dyeing solution; (iv) measuring the absorbance at the wavelength of 680 + / -10nm by using a microplate reader; wherein a standard curve is drawn according to the absorbance of the protein standard substances with different concentrations, and the absorbance of the protein extract is substituted into the standard curve to calculate the concentration of the ferrihemicin, so that the protective effect of the medicine on the lung qi and blood barrier is judged. The method disclosed by the invention can be used for simply, quickly and accurately evaluating the lung qi and blood barrier protection effect of the to-be-detected medicine.

The present application belongs to the technical field of quality control materials. Disclosed are a quality control material freeze-dried powder as well as a preparation method therefor and the use thereof. The quality control material freeze-dried powder is obtained by freeze-drying cell pellets containing cell fragments, the cell pellets being obtained by sectioning, deparaffinizing and hydrating a paraffin-embedded cell sample and then centrifuging same. The quality control material freeze-dried powder prepared by using the preparation method in the present application can reduce the loss of cell fragments during the freeze-drying process, so as to ensure the high recovery rate of cells, can avoid performance change or protein loss of cell fragments during the freeze-drying process, and can ensure that the immunohistochemical staining performance of reconstituted cell pellets after freeze-drying remains unchanged. The quality control material freeze-dried powder obtained by using the preparation method in the present application has extended storage time and facilitates transport, and the reconstituted cell suspension has good dispersibility, retains key target substances, and exhibits normal immunohistochemical staining performance.

The utility model relates to the technical field of cell extraction, and provides a centrifugal tube suitable for cell precipitation extraction, which comprises a first centrifugal tube, a second centrifugal tube and a fixing mechanism, the first centrifugal tube is detachably connected with the second centrifugal tube through the fixing mechanism, and scale marks are carved on the outer side walls of the first centrifugal tube and the second centrifugal tube; the fixing mechanism comprises a first connecting block and a second connecting block, the first connecting block is mounted on the first centrifugal tube, the second connecting block is mounted on the second centrifugal tube, and a plurality of limiting pieces are mounted at the top of the second connecting block. The centrifugal tube has the beneficial effects that the centrifugal tube is divided into two parts, namely the upper tube body and the lower tube body, and the two parts are detachably connected by adopting the fixing mechanism, so that the centrifugal tube can be easily assembled and disassembled, and the upper tube body can be slightly rotated to be separated from the lower tube body in cell precipitation extraction work, so that the extraction depth is reduced, and the extraction efficiency is improved. The bottom sediment can be more easily and completely taken out, and the cell sediment extraction work is facilitated.

The application belongs to the technical field of pharmaceutical analysis, and provides a method for quantitatively determining the concentration of PROTAC drugs in cells with high specificity, comprising the following steps: (1) selecting a cell strain expressing a PROTAC drug target; (2) culturing the cell strain to the logarithmic growth phase; (3) inoculating the cell suspension on a cell culture plate for culture; (4) adding the PROTAC drug to the cell culture plate, mixing and continuing to incubate; (5) removing the cell culture medium in the cell culture plate; dispersing the cell culture medium into a single cell suspension, centrifuging to discard the supernatant and obtain a cell precipitate; (6) lysing the cell precipitate, collecting the supernatant by ultracentrifugation, and performing ultra-high performance liquid chromatography-tandem mass spectrometry analysis; (7) preparing a blank cell suspension and a series of standard solutions of PROTAC drug concentrations, performing ultra-high performance liquid chromatography-tandem mass spectrometry analysis, and constructing a standard curve; and (8) using the standard curve to obtain the concentration of the PROTAC drug in the cells.

The present application relates to the technical field of medicine, and more particularly to a boronized cell membrane and a preparation method and application thereof.The preparation method of the boronized cell membrane comprises the following steps: adding tumor cells into a culture medium containing a monosaccharide-modified boron drug to perform incubation, collecting cell precipitates after the incubation is completed, and then extracting cell membranes, so that the boronized cell membrane is obtained.The present application provides a boronized cell membrane and a preparation method and application thereof.By using the monosaccharide-modified BSH, the obtained boron drug is anchored on the tumor cell membrane, and then the boron drug is delivered to the tumor tissue by the homologous targeting property of the cell membrane itself, so that the boronized cell membrane has high biocompatibility and helps to better achieve BNCT treatment.

Methods are provided to separate target cells from non-target cells in a liquid biopsy sample, based on the cells' sedimentation properties. One method involves providing a container comprising a volume Vd of a density medium and then adding a volume Vb of a liquid biopsy sample on top of the density medium; the volume Vb of the liquid biopsy sample is a sum of the volume of a liquid fraction Vp and the volume of cells Vc in the liquid biopsy sample. Next, the non-target cells in the liquid biopsy sample are allowed to sediment in a sedimentation layer at the bottom of the container; the sedimentation layer comprises a liquid volume Vremaining between the sedimented cells. Lastly, the supernatant comprising target cells is collected. In this method, the density of the density medium is higher than the density of the liquid biopsy sample but lower than the density of the non-target cells and the volume Vd of the density medium is at least equal to Vremaining. A method is provided to separate target cells from non-target cells in a liquid biopsy sample, based on the cells' sedimentation properties The method involves first providing a container comprising a volume Vd of a density medium. Next, a volume Vb of a liquid biopsy sample is added on top of the density medium. The non-target cells in the liquid biopsy sample are then allowed to sediment in the density medium. Next, the supernatant comprising target cells is collected. The volume Vb of the liquid biopsy sample is a sum of the volume of a liquid fraction Vp and the volume of cells Vc in the liquid biopsy sample, the volume Vd of the density medium is at least equal to the Vp. A kit to separate target cells from non-target cells in a liquid biopsy sample includes a container that contains a volume Vd of a density medium, and instructions to carry out the method.

The invention discloses an efficient universal mouse pancreatic tissue single-cell suspension preparation method, and belongs to the technical field of cell biology. Pancreas are rich in digestive enzymes, so that the treatment difficulty of in-vitro cells is high, and a high-quality single-cell suspension is difficult to prepare by a traditional method. The method comprises the steps of reagent preparation, tissue sampling, tissue dissociation, red cracking and cleaning, AOPI dyeing quality inspection and the like. Components and concentrations of a tissue enzymatic hydrolysate, a cell cleaning solution and a cell suspension are determined in reagent preparation; the acquisition method of the pancreatic tissue of the mouse is defined by tissue sampling; the tissue dissociation adopts a two-round digestion mode, and the cut tissue is subjected to enzymolysis by using a compound enzyme solution under specific conditions; the cell precipitates are treated through red cracking and cleaning; aOPI dyeing quality inspection is used for analyzing cell count and motility rate. According to the method, the culture medium containing specific components and the compound enzyme liquid are creatively used, the problem that pancreas single cells are difficult to dissociate can be effectively solved, the single-cell suspension with high motility rate, high purity and low adhesion is obtained, and the requirements of downstream single-cell experiments are met.

The application discloses a quality control product for human papillomavirus molecular detection and a preparation method thereof, and belongs to the technical field of biological products. After synthesizing 25 types of HPV full genomes of high-risk types (HPV16, HPV18, HPV26, HPV31, HPV33, HPV35, HPV39, HPV45, HPV51, HPV52, HPV53, HPV56, HPV58, HPV59, HPV66, HPV68, HPV73, HPV82) and low-risk types (HPV6, HPV11, HPV42, HPV43, HPV44, HPV81, HPV83), the HPV full genomes are connected into a pcDNA3.1(+) skeleton vector system, the HPV full genomes are integrated into the genome of a human ovarian cancer cell line ovcar3 respectively, and 25 strains of human ovarian cancer cell lines stably integrating the HPV full genomes are obtained. The new quality control product or kit of various types of simulated clinical samples such as genomic DNA, cell precipitate and cell suspension can be prepared and is suitable for various detection platforms. The human cell line quality control product stably integrating the full-length genomes of the 25 HPV types can more accurately determine the viral load and control the whole process of HPV detection.

The invention relates to a method for removing tissue fragments and impurities in a cell suspension, which comprises the following steps: S1, acquiring a cell suspension, centrifuging the cell suspension, removing supernate, and mixing with a cell precipitation resuspension to obtain a cell resuspension; s2, obtaining a gradient separation liquid and a centrifugal tube, adding the gradient separation liquid into the centrifugal tube, and adding the cell resuspension along the inner wall of the centrifugal tube to form a separation liquid layer and a cell suspension layer in the centrifugal tube; s3, carrying out gradient centrifugation on the centrifugal tube, taking the precipitate, and resuspending to obtain a purified cell suspension; wherein the gradient separation liquid comprises a Percoll solution or a sucrose solution. According to the technical scheme, fragments and impurities are separated by using a gradient density centrifugation method, and living cells, cell fragments and impurity fragments with relatively low density can be separated. And part of dead cells break cell membranes in the centrifugation process, become cell fragments and are removed along with density gradient centrifugation.

The utility model discloses a centrifuge tube for cell precipitation, which comprises a centrifuge tube body, the top of the centrifuge tube body is connected with a tube cover through threads, an inner strip is arranged in the centrifuge tube body, one side surface of the inner strip is attached to the inner wall of the centrifuge tube body, the other side of the inner strip is connected with an inner shaft, and the inner shaft is connected with a tube cover. A rotating shaft is rotationally embedded in the pipe cover, a rotating block is fixed to the top of the rotating shaft, and the top end of the inner shaft is fixed to the surface of the bottom of the rotating shaft; the rotating block, the rotating shaft, the inner shaft and the inner strips are designed, the rotating block is held to rotate the rotating shaft, the rotating shaft drives the inner strips on the inner shaft to rotate together, the inner strips scrape off cell sap attached to the inner wall of the centrifugal tube body, and the situation that the cell sap is attached to the inner wall of the tube body and cannot rotate centrifugally at a high speed in the liquid, and sedimentation of the cell sap is affected is avoided; and the inner shaft is straightened by the supporting sleeve, so that the inner shaft is prevented from skewing.

The invention relates to the field of biomedicine, in particular to a human-derived hepatic adenocarcinoma organoid culture method and application thereof. The invention provides an efficient organoid culture method which comprises the following steps: extracting a fresh lung-liver-like adenocarcinoma tissue sample, cleaning, trimming, cutting into pieces, dissociating and the like, mixing single-cell precipitate with matrigel, inoculating the mixture to a pore plate, and culturing by using a well-designed and optimized culture medium. The culture medium is based on advanced DMEM / F12, multiple key components such as GlutaMax, HEPES, B27, SB202190 and the like are added, the growth speed and the cell proliferation capacity of the organoid can be remarkably increased, culture of the human lung liver-like adenocarcinoma organoid can be effectively achieved, and the human lung liver-like adenocarcinoma organoid has the advantages of being simple in preparation method, low in cost and good in application prospect. The cultured human-derived lung-liver-like adenocarcinoma organ can stably express pathological characteristics, genetic characteristics and molecular biological characteristics of original tumor tissues, can reflect heterogeneity of lung-liver-like adenocarcinoma tumors, and provides an ideal model for in-depth study on pathogenesis, drug screening and personalized treatment of lung-liver-like adenocarcinoma.

This invention belongs to the field of biotechnology. Specifically, this invention discloses neural stem cells, their preparation methods and applications, including the following steps: (1) washing hippocampal tissue with PBS buffer, placing the washed hippocampal tissue in a digestive solution for digestion, adding a digestion termination solution to terminate digestion, centrifuging to obtain cell pellet, washing the cell pellet with PBS buffer, centrifuging, resuspending the cells in DMEM / F12 medium, and adjusting the cell concentration to 1×10⁻⁶. 4 (2) Primary culture; (3) Amplification culture. The amplification culture medium described in this invention can provide a carrier with viscosity during the culture of neural stem cells, promote the division and proliferation of neural stem cells, improve the activity and stability of neural stem cells, and increase the survival rate and cell number, and has broad application prospects.

The invention belongs to the technical field of biological detection, and particularly discloses a method for immunolocalization of intracellular protein by a scanning electron microscope based on nanogold labeling. The method comprises the following steps: collecting cells, cleaning the cells with PBS, and fixing the cells with paraformaldehyde; heating and melting agarose, preserving heat, gently resuspending the cell precipitate, curing and demolding to form a cell-agarose complex; embedding the cell-agarose complex, quickly freezing, slicing, attaching to the surface of ITO (Indium Tin Oxide) conductive glass, and drying at room temperature to obtain a slide; cleaning and drying the slide, and sequentially carrying out primary antibody incubation and nanogold secondary antibody incubation; positioning a soft agar region by adopting an SE mode of a scanning electron microscope and observing the morphology of the single cell; and detecting the position and the distribution form of the gold nanoparticles by adopting a BSE mode. According to the method, high-resolution, high-fidelity and high-specificity positioning of the intracellular protein is realized, the efficiency and accuracy of intracellular protein research are remarkably improved, and the method has important scientific value and application prospect.

This invention provides a kit, method, and single-cell library construction method for the dissociation of marine animal tissues. The dissociation method includes: S1) dissociating marine animal tissue samples at 8℃~12℃ using a cell dissociation buffer to obtain a single-cell suspension; S2) centrifuging the single-cell suspension and washing the single-cell precipitate obtained after centrifugation with a cell resuspension buffer to obtain single cells from the marine animal tissue sample; wherein, the cell dissociation buffer comprises a protease; the protease is Bacillus subtilis protease A; the concentration of sodium ions in the cell resuspension buffer is 300-800mM, and the concentration of potassium ions is 5-20mM.

The invention relates to a method of producing an ester of a fatty acid and a C1-C4 alcohol, comprising successive steps of: cultivating cells of a microalga strain on a growth medium, harvesting the cultivated cells and concentrating them to obtain a wet cell pellet, suspending said wet cell pellet in an hydroalcoholic solution comprising said C1-C4 alcohol, incubating the cell 5 suspension thus formed so as to form said ester of a fatty acid and a C1-C4 alcohol by transesterification and / or esterification of lipids produced by the microalga cells catalyzed by endogenous enzymes of said cells, and recovering said ester of a fatty acid and a C1-C4 alcohol from said cell suspension. The growth medium can advantageously be a dark fermentation effluent.

This invention proposes a method for preparing a single-cell suspension of porcine pancreatic cells and its application. The method includes the following steps: S1, taking porcine pancreatic tissue, removing surface fat, and mincing the tissue; S2, washing the tissue fragments, and then placing the tissue into a mixed enzyme dissociation solution for the first digestion; S3, after the first digestion, collecting the supernatant, and adding the remaining tissue to the mixed enzyme dissociation solution for a second digestion; S4, after the second digestion, collecting the supernatant and combining it with the supernatant from step S3, and filtering; S5, centrifuging the filtered dissociation solution, discarding the supernatant, and resuspending the cell pellet to obtain a cell suspension; the mixed enzyme dissociation solution includes trypsin, collagenase I, elastase, lipase, DNase I, heparin, sodium sulfate, and DH Kans buffer. The enzyme mixture of this invention has the effects of removing intercellular matrix, reducing cell aggregation, protecting cell membrane morphology, and reducing cell damage.

The invention discloses a preparation method and application of a monoclonal cell with positive CAR (chimeric antigen receptor). The cell preparation method comprises the following steps: acquiring tumor cells and a lentiviral vector containing a target CAR gene, and transferring the tumor cells and the lentiviral vector to a 24-hole culture plate for mixed transfection culture; after culture is finished, centrifuging the culture solution, removing supernatant, and resuspending cell precipitate by using a culture medium; sorting the cell suspension, transplanting the sorted cells into a 96-well plate, and culturing for 7-14 days; and after the culture is finished, centrifuging the culture solution, removing the supernatant, and washing the cell precipitate with PBD to obtain the CAR-positive monoclonal cell. A series of cell strain lines capable of stably expressing the CAR gene are constructed through lentivirus transfection, a stable positive quality control product is provided for CAR-T cell detection, standardized and systematized operation of CAR-T detection is improved, it is ensured that a CAR-T detection result is accurate, credible and reliable, and it is ensured that CAR-T cells are safely and effectively used clinically.

This invention provides a method for producing full-length antibodies in Escherichia coli at a yield of approximately 200 mg / L or more. [Solution] A method for producing a full-length antibody comprising a heavy chain (HC) and a light chain (LC), comprising culturing Escherichia coli cells expressing the coding sequence of the HC and the coding sequence of the LC in a culture medium under conditions acceptable for producing the HC and the LC, wherein the full-length antibody is produced in an amount of at least about 200 mg per liter of the culture medium, or the weight percentage of the produced full-length antibody relative to the weight of the cell pellet produced from the Escherichia coli cells is in the range of 0.05% to 20%.

This invention provides a method for producing cell tissue structures that are highly stable and reproducible. [Solution] The number of cells in the cell suspension and the cell diameter are counted (S10). The cell volume is calculated from the number of cells and cell diameter obtained in the counting step (S20). A cell pellet is obtained by centrifuging the cell suspension containing the cells whose cell volume has been calculated (S30). The cell pellet volume, which is the total volume of the cell pellet, is calculated (S40). Based on the cell pellet volume, a solvent containing biomaterials is added to the cell pellet so that the cells have a desired cell volume fraction, and a mixture is obtained (S50).