The invention relates to a microfluid
cell processing chip comprising a bottom layer and a top layer. A snakelike
pipe and a
cell operation chamber are disposed in the top layer, and two solution inflow channels are disposed on the inflow-port of the snakelike
pipe. A protective agent entrance is disposed on the inlet
pipe of one of the inflow channels, and a
buffer solution entrance is disposed on the inlet pipe of another inflow channel. A solution outflow channel of the snakelike pipe is connected with the
cell operation chamber, and the cell operation chamber is provided with a solution exit, a waste fluid outflow channel and a cell access channel. A row of cylindrical barriers are disposed on each side of the cell operation chamber, and the barriers are used for limiting the cell in the operation chamber, so that the cell can not be washed away by the protective agent. In practical application, operator uses the refitted cell mouth-sucking device to blow or suck the cell in or out the
chip cell operation chamber directly. The microfluid
cell processing chip and application method thereof can make the concentration of protective agent change continuously when adding or removing the
cryoprotectant of animal cell with large volume. The method has direct and simple operation, and has less damage for cell.