12 results about "Soft agar" patented technology

The invention discloses a method for improving biocontrol photosynthetic bacteria through a far-source hybridization and genome rearrangement technology. The method comprises the following steps: inoculating a rhodopseudomonas palustris GJ-22 seed into a photosynthetic bacteria liquid culture medium for culturing; inoculating the bacillus subtilis KY-2 into an LB liquid culture medium for culturing; the cultured GJ-22 is taken and subjected to treatment and centrifugation, and then a GJ-22 protoplast is obtained; kY-2 is taken to be treated, and KY-2 protoplast is obtained after centrifugation; fusing the obtained GJ-22 protoplast and KY-2 protoplast, diluting a photosynthetic bacterium hypertonic liquid culture medium, and regenerating on a photosynthetic bacterium soft agar culture medium to obtain a fused strain; a fusion strain is screened out through an induced tobacco core leaf anti-TMV test to serve as a starting strain for genome rearrangement. Preparing protoplast from the original strain; dividing the prepared protoplast into two groups, and respectively carrying out ultraviolet inactivation and thermal inactivation; respectively fusing the inactivated protoplasts, and regenerating to obtain fusant strains; finally, a target strain with genetic stability is screened through a fusant strain induced tobacco core leaf anti-TMV test.

The invention relates to the fields of epigenetic regulation and tumor treatment, and aims to evaluate that the cell distribution of the mutant is consistent with that of H3 by constructing Bas-2B (H3K9R) and Bas-2B (H3K18R) stably transfected cell strains and analyzing the cell positioning condition of the H3K9R / H3K18R mutant through an immunofluorescence technique. According to the present invention, a Western blotting technology is adopted to detect the H3K9 / H3K18 lactic acid modification level of Bas-2B (H3K9R) and Bas-2B (H3K18R) cells compared to control cells so as to verify the influence of the H3K9R / H3K18R mutant on the H3K9 / H3K18 lactic acid modification; after Bas-2B (H3K9R), Bas-2B (H3K18R) cells and control cells of the Bas-2B (H3K9R) and the Bas-2B (H3K18R) cells are subjected to nickel compound exposure treatment, the influence of the H3K9R / H3K18R mutant on the nickel compound induced lung cancer is evaluated through a soft agar cloning formation experiment. An AGS cell line stably transfecting H2BK16R is constructed, and the cell localization condition of the H2BK16R mutant is analyzed through an immunofluorescence technology. According to the present invention, a Westernbloting technology is adopted to detect the lactic acid modification level of AGS (H2BK16R) cells so as to verify the influence of the H2BK16R mutant on the H2BK16 lactic acid modification; the influence of the H2BK16R on the malignant proliferation ability of the gastric cancer is researched through a soft agar clone formation experiment.

The invention relates to a microbial biofilm cultivation method comprising the use of a soft agar hydrogel substrate and one or more stains for detection and / or quantification of one or more microbial components and / or characteristics. The invention further relates to the use of the microbial biofilm cultivation method for screening of one or more antimicrobial compounds.

The invention discloses a non-tumorigenic MDCK cell strain, a screening method and application. The non-tumorigenic MDCK cell strain is MDCK-CA027 (the preservation number is CCTCC (China Center for Type Culture Collection) NO: C2023329). The tumor formation rate of the cell strain is 0% when the cell strain is observed for at least 16 weeks in a nude mouse in-vivo tumor formation test, and the cell strain has good sensitivity to various influenza viruses (such as H1N1, H3N2, BY and BV). The invention also provides a method for screening the cell strain. The method comprises the following steps: preparing a single-cell suspension, carrying out limited dilution to obtain monoclone, carrying out enlarged culture, carrying out virus sensitivity detection, carrying out in-vitro tumorigenicity related detection (clone formation test / soft agar test), carrying out nude mouse in-vivo tumorigenicity test and the like. The non-tumorigenic MDCK cell strain is high in safety, can efficiently support influenza virus replication, can be safely and effectively applied to influenza vaccine production and influenza virus amplification, overcomes the tumorigenic risk of traditional MDCK cells, and has important scientific significance and application prospects.

The application discloses application of a bruceine A in preparation of a medicine for treating ovarian cancer and belongs to the technical field of cancer medicines. The embodiment of the application proves the application of the bruceine A in preparation of the medicine for treating the ovarian cancer, and the dosage of the bruceine A is 200 mg / kg. The embodiment of the application proves that the bruceine A has a significant proliferation inhibiting effect on human ovarian cancer cells; the soft agar colony formation of the human ovarian cancer cells is significantly lower than that of a control group; the bruceine A significantly inhibits the invasion ability of the human ovarian cancer cells; the bruceine A can significantly reduce the volume and weight of the ovarian cancer tumor after being administered at a dosage of 200 mg / kg, has a significant inhibiting effect on the ovarian cancer tumor, and can be applied in preparation of the medicine for treating the ovarian cancer.

According to the Escherichia coli engineering strain with enhanced athletic ability and the construction method and application thereof, wild Escherichia coli MG1655 is taken as a transformation starting point, and expression of flagellum power regulation protein CheZ is specifically enhanced, so that the chemotactic athletic ability of the strain is remarkably enhanced, the migration range of the transformed strain on a soft agar plate is expanded compared with that of the wild Escherichia coli MG1655, and the activity of the transformed strain on the soft agar plate is improved. And the rapid growth characteristic of the original host is maintained. The strain can break through the space motion limitation of traditional microorganisms in soil remediation, achieves targeted migration of deep soil pollutants, and is suitable for efficient bioremediation of heavy metal and organic pollutants in a heterogeneous soil environment.

The invention belongs to the technical field of biological detection, and particularly discloses a method for immunolocalization of intracellular protein by a scanning electron microscope based on nanogold labeling. The method comprises the following steps: collecting cells, cleaning the cells with PBS, and fixing the cells with paraformaldehyde; heating and melting agarose, preserving heat, gently resuspending the cell precipitate, curing and demolding to form a cell-agarose complex; embedding the cell-agarose complex, quickly freezing, slicing, attaching to the surface of ITO (Indium Tin Oxide) conductive glass, and drying at room temperature to obtain a slide; cleaning and drying the slide, and sequentially carrying out primary antibody incubation and nanogold secondary antibody incubation; positioning a soft agar region by adopting an SE mode of a scanning electron microscope and observing the morphology of the single cell; and detecting the position and the distribution form of the gold nanoparticles by adopting a BSE mode. According to the method, high-resolution, high-fidelity and high-specificity positioning of the intracellular protein is realized, the efficiency and accuracy of intracellular protein research are remarkably improved, and the method has important scientific value and application prospect.

The invention discloses a primary cell and a progeny cell of human brain glioma and application of the primary cell and the progeny cell. The invention provides a human brain glioma primary cell which is a human brain glioma primary cell Glioma-2, and the preservation number of the human brain glioma primary cell Glioma-2 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: C202420. The human brain glioma primary cell Glioma-2 provided by the invention can form clone in soft agar, and shows different drug toxic reactions to common chemotherapeutic drugs (cis-platinum, carboplatin, etoposide, carmostine and irinotecan) for brain glioma. The human brain glioma primary cell Glioma-2 provided by the invention can be used for analyzing the pathogenesis of brain glioma in vitro and in vivo and detecting related characteristics such as drug sensitivity in vitro, and an experimental material closer to clinical tumor biological characteristics is provided for research of human brain glioma.

The invention discloses a malignant transformed cell strain induced by benzo [g, h, i] perylene and application thereof. The cell strain is preserved in Guangdong Microbial Culture Collection Center on June 27, 2024, and the preservation number is GDMCC No: 64810. The inventor adopts benzo [g, h, i] perylene with environmental exposure concentration to continuously act on human lung bronchial epithelial cells to construct an in-vitro malignant transformed cell strain, the in-vitro malignant transformed cell strain is successfully constructed through identification of a soft agar clone formation experiment and a mouse-loaded tumorigenesis experiment, and the model is subjected to multiplication capacity, apoptosis, period, invasion and migration capacity and DNA damage degree detection, so that the in-vitro malignant transformed cell strain is obtained. The general characteristics of the metabolite cells are met. The in-vitro malignant transformed cell strain / cell model provides more intuitive evidence for the carcinogenicity research of benzo [g, h, i] perylene and provides an experimental basis for the subsequent research of the carcinogenic mechanism of benzo [g, h, i] perylene.

The application relates to application of a glutamine metabolism inhibitor V-9302 in preparation of a drug for treating diseases caused by cell malignant transformation of arsenic. The application is characterized in that the glutamine metabolism inhibitor V-9302 is used to inhibit the cell malignant transformation caused by arsenic, the malignant transformation phenotype of cells is reversed after the glutamine metabolism is inhibited, the cell doubling time is increased, the migration rate and the soft agar colony formation rate are reduced, and it is proved that the enhancement of the glutamine metabolism promotes the cell malignant transformation mediated by the synthesis of glutathione.

The invention relates to a new application of cefathiamidine in preparation of antitumor drugs, in particular to an application in preparation of drugs for resisting lung cancer, esophageal cancer, breast cancer and gastric cancer. Experimental results show that the compound has a remarkable inhibition effect on cell proliferation of lung cancer cell lines A549 and H460, an esophageal cancer cell line KYSE450, a breast cancer cell line BT549 and a gastric cancer cell line BGC803, and flat plate cloning formation and soft agar cloning formation of the lung cancer cell lines A549 and H460; the cell cycle G2 phase retardation of lung cancer cell lines A549 and H460 is promoted. Therefore, the cefathiamidine can be used as a lead compound for developing a new anti-tumor drug, and can also be used for preparing anti-tumor drugs, especially anti-lung cancer, anti-esophageal cancer, anti-breast cancer and anti-gastric cancer drugs, and a new source is provided for seeking the anti-lung cancer, anti-esophageal cancer, anti-breast cancer and anti-gastric cancer drugs.

This invention discloses a double-layer aerogel packaging pad, its preparation method, and its application. The aerogel packaging pad includes an upper agar layer, a lower gelatin layer, and a natural antibacterial agent. The aerogel pad of this invention comprises a hydrophilic agar layer and a hydrophobic gelatin layer. The soft agar layer wraps around fruits and vegetables, providing ample cushioning, while the gelatin layer, with its certain mechanical strength, provides sufficient resistance to compressive stress, thereby preventing mechanical damage to fruits and vegetables caused by external collisions or compression during transportation. The aerogel packaging pad not only has extremely high water absorption and water vapor absorption capacity but also possesses an intelligent humidity regulation function of "rapid absorption and slow release." It maintains the humidity inside the packaging pad at a suitable level, preventing the fresh fruits and vegetables from wilting due to dehydration and inhibiting the growth of microorganisms caused by excessive moisture, significantly extending the shelf life of fruits and vegetables.