7 results about "Soft agar" patented technology

The invention relates to a microbial biofilm cultivation method comprising the use of a soft agar hydrogel substrate and one or more stains for detection and / or quantification of one or more microbial components and / or characteristics. The invention further relates to the use of the microbial biofilm cultivation method for screening of one or more antimicrobial compounds.

The invention discloses a non-tumorigenic MDCK cell strain, a screening method and application. The non-tumorigenic MDCK cell strain is MDCK-CA027 (the preservation number is CCTCC (China Center for Type Culture Collection) NO: C2023329). The tumor formation rate of the cell strain is 0% when the cell strain is observed for at least 16 weeks in a nude mouse in-vivo tumor formation test, and the cell strain has good sensitivity to various influenza viruses (such as H1N1, H3N2, BY and BV). The invention also provides a method for screening the cell strain. The method comprises the following steps: preparing a single-cell suspension, carrying out limited dilution to obtain monoclone, carrying out enlarged culture, carrying out virus sensitivity detection, carrying out in-vitro tumorigenicity related detection (clone formation test / soft agar test), carrying out nude mouse in-vivo tumorigenicity test and the like. The non-tumorigenic MDCK cell strain is high in safety, can efficiently support influenza virus replication, can be safely and effectively applied to influenza vaccine production and influenza virus amplification, overcomes the tumorigenic risk of traditional MDCK cells, and has important scientific significance and application prospects.

The application discloses application of a bruceine A in preparation of a medicine for treating ovarian cancer and belongs to the technical field of cancer medicines. The embodiment of the application proves the application of the bruceine A in preparation of the medicine for treating the ovarian cancer, and the dosage of the bruceine A is 200 mg / kg. The embodiment of the application proves that the bruceine A has a significant proliferation inhibiting effect on human ovarian cancer cells; the soft agar colony formation of the human ovarian cancer cells is significantly lower than that of a control group; the bruceine A significantly inhibits the invasion ability of the human ovarian cancer cells; the bruceine A can significantly reduce the volume and weight of the ovarian cancer tumor after being administered at a dosage of 200 mg / kg, has a significant inhibiting effect on the ovarian cancer tumor, and can be applied in preparation of the medicine for treating the ovarian cancer.

According to the Escherichia coli engineering strain with enhanced athletic ability and the construction method and application thereof, wild Escherichia coli MG1655 is taken as a transformation starting point, and expression of flagellum power regulation protein CheZ is specifically enhanced, so that the chemotactic athletic ability of the strain is remarkably enhanced, the migration range of the transformed strain on a soft agar plate is expanded compared with that of the wild Escherichia coli MG1655, and the activity of the transformed strain on the soft agar plate is improved. And the rapid growth characteristic of the original host is maintained. The strain can break through the space motion limitation of traditional microorganisms in soil remediation, achieves targeted migration of deep soil pollutants, and is suitable for efficient bioremediation of heavy metal and organic pollutants in a heterogeneous soil environment.

The application relates to application of a glutamine metabolism inhibitor V-9302 in preparation of a drug for treating diseases caused by cell malignant transformation of arsenic. The application is characterized in that the glutamine metabolism inhibitor V-9302 is used to inhibit the cell malignant transformation caused by arsenic, the malignant transformation phenotype of cells is reversed after the glutamine metabolism is inhibited, the cell doubling time is increased, the migration rate and the soft agar colony formation rate are reduced, and it is proved that the enhancement of the glutamine metabolism promotes the cell malignant transformation mediated by the synthesis of glutathione.

The invention relates to a new application of cefathiamidine in preparation of antitumor drugs, in particular to an application in preparation of drugs for resisting lung cancer, esophageal cancer, breast cancer and gastric cancer. Experimental results show that the compound has a remarkable inhibition effect on cell proliferation of lung cancer cell lines A549 and H460, an esophageal cancer cell line KYSE450, a breast cancer cell line BT549 and a gastric cancer cell line BGC803, and flat plate cloning formation and soft agar cloning formation of the lung cancer cell lines A549 and H460; the cell cycle G2 phase retardation of lung cancer cell lines A549 and H460 is promoted. Therefore, the cefathiamidine can be used as a lead compound for developing a new anti-tumor drug, and can also be used for preparing anti-tumor drugs, especially anti-lung cancer, anti-esophageal cancer, anti-breast cancer and anti-gastric cancer drugs, and a new source is provided for seeking the anti-lung cancer, anti-esophageal cancer, anti-breast cancer and anti-gastric cancer drugs.

This invention discloses a double-layer aerogel packaging pad, its preparation method, and its application. The aerogel packaging pad includes an upper agar layer, a lower gelatin layer, and a natural antibacterial agent. The aerogel pad of this invention comprises a hydrophilic agar layer and a hydrophobic gelatin layer. The soft agar layer wraps around fruits and vegetables, providing ample cushioning, while the gelatin layer, with its certain mechanical strength, provides sufficient resistance to compressive stress, thereby preventing mechanical damage to fruits and vegetables caused by external collisions or compression during transportation. The aerogel packaging pad not only has extremely high water absorption and water vapor absorption capacity but also possesses an intelligent humidity regulation function of "rapid absorption and slow release." It maintains the humidity inside the packaging pad at a suitable level, preventing the fresh fruits and vegetables from wilting due to dehydration and inhibiting the growth of microorganisms caused by excessive moisture, significantly extending the shelf life of fruits and vegetables.