Cellulose oligosaccharide transporter as well as preparation method and application thereof

A technology of cellooligosaccharides and transport proteins, applied in the field of genetic engineering, can solve the problems of waste of resources, low utilization rate of fiber, low transfer efficiency of cellooligosaccharides, etc., and achieve the effect of improving transfer efficiency
CN114807201APending Publication Date: 2022-07-29GUANGZHOU INST OF ADVANCED TECH CHINESE ACAD OF SCI
3 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
GUANGZHOU INST OF ADVANCED TECH CHINESE ACAD OF SCI
Publication Date
2022-07-29

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a cellooligosaccharide transporter as well as a preparation method and application thereof, the preparation method of the cellooligosaccharide transporter engineering strain comprises the following steps: step 1, taking a wild type SP-LacY as an initial bacteriophage, taking a visual bacteriophage-assisted continuous directed evolution system engineering bacterium containing an AP1 plasmid, an AP2 plasmid and an MP1 plasmid as a host, and preparing a cellooligosaccharide transporter engineering strain; culturing phage spots by using LB or M9 soft agar containing cellooligosaccharides as a culture medium; step 2, continuing to perform multi-round evolution culture on the obtained phage plaque, sequencing the phage plaque obtained in each round of evolution to verify the mutant accumulation trend, stopping evolution until no new mutant is formed, and finally obtaining proteins with different mutation sites; and step 3, combining different mutation sites of the protein obtained in the step 2 to construct different LacY mutant expression vectors, and transforming the LacY mutant expression vectors into host bacteria to obtain the LacY mutant. The cellooligosaccharide transporter provided by the invention can better ferment and utilize cellulose and cellooligosaccharide.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, in particular to a cello-oligosaccharide transporter and a preparation method and application thereof. Background technique

[0002] Cellulose is the most abundant natural polymer compound in the world, most of which are synthesized by green plants through photosynthesis. Currently using cellulosic to produce industrial by-products, such as alcohol, firstly, the cellulosic must be physically and chemically treated or pretreated with cellulase to degrade the cellulosic into glucose, which is then used by microorganisms to produce industrial by-products; in fact, the pretreated cellulosic The degradation products also contain a large amount of cello-oligosaccharides such as cellobiose and cellotriose. However, due to the lack of specific cello-oligosaccharide transporters in microorganisms, microorganisms cannot efficiently utilize cello-oligosaccharides, resulting in waste of resources...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More