41 results about "Well differentiated" patented technology

The present invention is directed to a method for the determination of a target nucleic acid using a special control nucleic acid, a method for the amplification of a partial sequence of said target nucleic acid using primers, a special control and a kit containing said control. The sequence of these control nucleic acids are at least in part parallel-complementary to the sequence of the target nucleic. These controls have similar properties as the target nucleic acid in hybridization and amplification methods, but can be well differentiated from the target nucleic acid by their different sequence.

Use of Mycobacterium brumae for the treatment of bladder cancer. The present invention relates to the treatment of bladder cancer, preferably superficial, non-invasive bladder cancer by using mycobacteria Mycobacterium brumae. Therapy described in this invention is particularly effective in grade 1 (well differentiated) and grade 2 (moderately differentiated) tumor cells. This makes it possible to treat the disease, which is still in an early stage, by reducing the risk of disease progression to a more harmful stage and/or preventing the risk of metastasis.

The present invention relates to methods of reducing a level of a target mRNA in a well-differentiated airway epithelial cell by contacting the cell with a sensitizing agent followed by contacting the cell with a therapeutic RNAi agent.

Phage display was used to screen peptide libraries that distinguish between well-differentiated (HCT116) and poorly-differentiated colon carcinoma cells (HT29). The screening protocol used selection and subtraction on intact, viable cells, resulting in phage libraries exhibiting high binding selectivity for the poorly-differentiated HT29 cells. A nine amino acid, disulfide-constrained peptide (RPM) was identified that selectively bound and was internalized into colon cancer cells. The peptide may be used to detect colon cancer cells and also may be used to selectively deliver therapeutic agents to the cells.

The invention belongs to the technical field of image analysis, in particular to a microscopic image analysis method of a cervical cancer tissue based on a graph theory. The microscopic image analysismethod of the cervical cancer tissue based on the graph theory includes the following steps: collecting microscopic image data of cervical cancer tissue, segmenting each original image by different algorithms, and fusing the segmentation results to obtain fused images; according to the morphological and textural features of the nuclei, the fusion images were classified into three categories: highly differentiated, moderately differentiated and poorly differentiated; comprehensive assessment of classification results are performed. The present application uses fused segmentation algorithms toenhance accuracy, form a complete classification process, and make classification evaluations. Pathological images of cervical cancer can be divided into three types: well differentiated, moderately differentiated and poorly differentiated according to the spatial structure of the nucleus, which can be used in the daily practice of histologists to speed up the time of diagnosis and improve the accuracy of diagnosis.

A horn antenna combining horizontal and vertical corrugations. It is made up of two well differentiated parts, the first part being an antenna with horizontal corrugations, i.e. parallel to the axis of propagation, and a second part with vertical corrugations, i.e. transverse to the axis of propagation. The aperture of the arrangement of the corrugations, in the two parts, can preferably follow linear or Gaussian functions.

The invention provides a rhizosphere simulated culture device and a method for culturing rhizosphere by using the same, belongs to the technical field of agriculture and aims to solve the problems that by the conventional method, the plants are cultured in one type of culture box, the rhizosphere environment can only be studied as a whole, the microenvironments of different rhizospheres cannot be well differentiated, and the workload is huge, the interference factors are too many, the error is big, the precision is low and the like. A culture bowl (1) of the rhizosphere simulated culture device is provided with seeding holes (2), an organic glass plate (3) is fixed on the bottom surface of the culture bowl (1), the culture bowl (1), on which the organic glass plate (3) is fixed, is put into a groove (5), and the groove (5) is embedded into a base (4). The culture method comprises the following steps of: 1, using the rhizosphere culture device; 2, loading soil; 3, fixing the organic glass plate; 4, sowing; 5, placing the culture bowl into the groove; and 6, culturing. The rhizosphere micro-ecological culture device can be used for detecting the micro-ecological environment of the rhizosphere of plants.

The present invention discloses a human well-differentiated liver cancer cell line HL1017, wherein a preservation number is CCTCC NO:C201357, and the cell line has the most important functions of synthesis, metabolism and detoxification of liver. In addition, the present invention further discloses a construction method of the human well-differentiated liver cancer cell line HL1017.

Disclosed are pieces that are produced by sintering, which have well-differentiated surface finish areas including rough areas resulting from the sintering process, the roughness being determined by the size of grain used in sintering, and polished areas that have undergone a three-dimensional machining process. Also, dislosed is a method that, after obtaining a file containing a representation of the piece to be produced, comprises: selecting the parts to be raised; producing the piece by means of sintering; and subsequently subjecting the piece to automatic, semi-automatic or directed programming generation so that, by means of machining, the raised parts are reduced. Consequently, the resulting piece is economical in terms of costs and exhibits a very high degree of precision in the machined areas, a tolerance 9, and improved finish, texture and finishing in the sensitive areas of the piece.

The invention discloses a method for transmitting the antenna information of a physical broadcast channel and is applied in a wireless communication system, and according to the transmission antenna number of the physical broadcast channel and which time the current sending belongs to in a period, a sending end selects a corresponding scrambling sequence for implementing the scrambling of original information bits, generates a cyclical redundancy check (CRC) code, and sends out the CRC code together with the data; wherein, two antennas and four antennas are corresponding to different scrambling sequences. The method of the invention can well detect the antenna information of the physical broadcast channel and is characterized in that the two situations with two antennas and four antennas are well differentiated, thus ensuring the decoding performance of other physical channels.

The invention discloses a method for transmitting the antenna information of a physical broadcast channel, and a sending end arranges the original information bits in sequence according to the number of the sending antennas of the physical broadcast channel, generates cyclical redundancy check (CRC) bits according to the ordering result, and sends out the CRC bits together with the original information bits; wherein, the method adopts different ordering ways in the situations with two antennas or four antennas. The method of the invention can well detect the antenna information of the physical broadcast channel and is characterized in that the two situations with two antennas and four antennas are well differentiated, thus ensuring the decoding performance of other physical channels.

As a result of immunohistochemical staining of human cancer pathological tissue sections and normal tissue sections with various marker candidates, the antibody against the SLC38A4 protein, out of 80 spontaneously developed types of antigen-specific antibodies against a transporter, was found to react specifically to esophageal cancer tissue and in particular to show high reactivity in esophageal cancer tissue in early well-differentiated stages.

The invention relates to the technical field of immunization, in particular to a polyinosinic: polycytidylic acid copolymer (Poly I: C). The polyinosinic: polycytidylic acid copolymer is a ligand of atype-3 Toll-like receptor in an animal body, can mediate a series of immune reactions of the body after TLR-3 is activated, and has a good promoting effect on specific immunity and non-specific immunity of the body. Compared with a control group, DC-CIK induced by the polyinosinic: polycytidylic acid copolymer has higher proliferation, high differentiation (especially CD3+CD4-CD8+, CTL) and hightumor killing activity. The autologous efficient DC-CIK induced by the polyinosinic: polycytidylic acid copolymer and other immune cells are applied to treatment of clinical tumor patients. The polyinosinic: polycytidylic acid copolymer aims to recover or improve the immune function of the tumor patients, and the immune system of the body is improved to kill and inhibit proliferation of tumor cells. The tumor cell load is reduced, tiny residual lesions are removed, or the proliferation mode of residual tumor/cancer cells is obviously inhibited, factors such as relapse and metastasis are eliminated, the cure possibility is increased, the survival time is prolonged, and the life quality is improved. Therefore, the purpose of treating tumors/cancers is achieved.

The invention provides a group of molecular markers of well-differentiated early liver cancer and a use thereof. The group of molecular markers of well-differentiated early liver cancer comprises phosphoglucomutase-1(PGM1) and sequestra sequestrum-1 (SQSTM1). The invention further provides a use of phosphoglucomutase-1 and sequestra sequestrum-1 as a molecular marker combination of early liver cancer in preparation of liver cancer clinical diagnostic reagents. The invention further provides a liver cancer diagnostic kit, wherein the liver cancer diagnostic kit is characterized by comprising a phosphoglucomutase-1 antibody and a sequestra sequestrum-1 antibody. The invention has the advantage that relatively high detection sensitivity and specificity are provided.

The invention relates to the technical field of molecular biology and discloses a method for identifying material sources of meat products based on clone and conventional sequencing. The method comprises the following steps: (1) extracting to-be-detected genome DNA; (2) designing and synthesizing a forward primer and a backward primer; (3) carrying out PCR amplification on the genome DNA, and detecting and recycling amplification products; (4) carrying out TA cloning on the PCR amplification products to obtain positive clones, selecting single clones, and carrying out sequencing; and (5) removing carrier sequences at two ends in a sequencing result to obtain COI sequence information of to-be-detected meat products, and therefore, identifying the material sources of the meat products. By virtue of the method, the material compositions and sources of the meat products can be identified, and species can be well differentiated by virtue of difference among base sequences in the COI gene sequence, so that the method for identifying the material sources of the meat products has relatively high accuracy.

The invention provides a rotating machine fault diagnosis method based on a peak index. A peak index is constructed based on the idea of signal separation to overcome the shortcomings of the traditional peak index diagnosis technology and improve the accuracy and reliability of diagnosis. Based on a standard vibration signal, vibration signals acquired in real time are divided into trouble-free vibration signals and mixed signals. A peak index is constructed using mixed signals and trouble-free vibration signals, the peak index is more sensitive to fault diagnosis, and weak fault feature signals can be better detected through signal change. There is less overlap between the value range of peak index under normal operation of rotating mechanical equipment and the value range of peak index under failure of the rotating mechanical equipment, and the peak index changes apparently under different conditions. The peak index is sensitive to the fault of an eccentric shaft, and the fault can be well differentiated from other faults. Rotating machine fault diagnosis is highly precise and reliable.

The invention discloses a tumor tissue in-vitro pathological staining kit. The tumor tissue in-vitro pathological staining kit is a kit developed aiming at leading Cetuximab to be used in new applications of tumor tissue in-vitro pathological staining, experiments verification finds that after the Cetuximab is subjected to fluorescent labeling and then used for tumor tissue in-vitro pathological staining, normal tissues and tumor tissues can be well distinguished, well-differentiated and poorly-differentiated tumor tissues can be distinguished to certain extent, and the kit can be used for tumor detection, thus realizing new use for approved drugs.

The invention discloses a monoclonal antibody resisting the ovarian cancer and application thereof. A hybridoma cell strain NM003-1 secreting the monoclonal antibody resisting the human ovarian cancer is preserved in China Center for Type Culture Collection on September 26, 2011 with the preservation number of CCTCC NO: C201174. The hybridoma cell strain NM003-1 secretes the monoclonal antibody NJ003-1 resisting the human ovarian cancer. Expression of a specific antigen of the monoclonal antibody NJ003-1 in ovarian cancer tissue is higher than that in ovarian benign disease tissue; the positive expression rate of the antigen in a poorly differentiated patient is higher than that in a well differentiated patient. The monoclonal antibody NJ003-1 can effectively inhibit clone formation of ovarian carcinoma cells SK-OV-3 in soft agar and can remarkably inhibit growth of human ovarian cancer transplantation tumors in a nude mouse. Obviously, the monoclonal antibody is expected to become medicine for treating the ovarian cancer or to be prepared into a diagnostic reagent for detecting the ovarian cancer.

The present invention relates to a medium composition for in vitro culturing Polygoni Multiflori Radix and a method for mass production of seedlings using the same. In detail, media that exhibited excellent growth rates upon in vitro culturing Polygoni Multiflori Radix were selected from among 10 different media, and the selected media, when supplemented with the plant growth regulator kinetin incombination with IAA or NAA, were found to allow tuberous roots of seedlings to differentiate well. Further, it was found that culturing in the media under the control of contents of inorganic salts therein led to the growth of plants having well-differentiated tuberous roots and that the plants were well-acclimated to soil.