Method for preparing jarosite through microbial conversion

A technology for microbial transformation and jarosite, applied in the field of microbial transformation to prepare jarosite, can solve the problems of sparse jarosite crystals, complicated process conditions, and other impurities, etc., and achieves convenient preparation and high biological safety. sexual effect

Inactive Publication Date: 2014-04-23
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, jarosite crystals are rare and often accompanied by other impurities
Many studies on the synthesis of jarosite by chemical methods require high temperature and high pressure, complex process conditions, or need to react for several months at normal temperature and pressure, and contain colloidal red amorphous hydroxysulfate ferric impurities, so they are all subject to application. extremely restrictive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 A method for preparation of jarosite by microbial transformation, comprising the following steps:

[0024] (1) Preparation of 9K medium: Mix liquid a and liquid b at a volume ratio of 7mL: 3mL to obtain 9K medium.

[0025] in:

[0026] Liquid a refers to dissolving 3.00g of ammonium sulfate, 0.10g of potassium chloride, 0.50g of dipotassium hydrogen phosphate, 0.50g of magnesium sulfate, and 0.01g of calcium nitrate in 700mL of distilled water, and adjusting the pH value to 1.5~1.9 with concentrated sulfuric acid solution.

[0027] Liquid b refers to a solution obtained by dissolving 44.70 g of ferrous sulfate in 300 mL of distilled water.

[0028] ⑵ Preparation of biotransformation medium: Mix liquid A and liquid B at a volume ratio of 7mL: 3mL.

[0029] in:

[0030] Liquid A refers to dissolving 3.00g of ammonium sulfate, 0.10~0.20g of potassium chloride, 0.50~1.00g of dipotassium hydrogen phosphate, 0.50g of magnesium sulfate, and 0.01g of calcium ...

Embodiment 2

[0041] Example 2 A method for preparation of jarosite by microbial transformation, comprising the following steps:

[0042] ⑴Preparation of 9K medium; ⑵Preparation of biotransformation medium; ⑶Preparation of soft agarose medium; Example 1 .

[0043] (5) Strain activation: the Leptospira ferrophilum BYQ obtained in step (4) Leptospirillum ferriphilum BYQ) strains were inoculated into shake flasks containing 9K medium at 12% inoculum amount, placed on a constant temperature shaker, and shaken at a rate of 100 r / min for 60 hours at 35°C to obtain Bacteria activation solution.

[0044] Wherein: the bottling capacity of 9K medium in the shake flask is 60%;

[0045] ⑹Biotransformation: Inoculate the strain activation liquid into the shake flask containing the biotransformation medium according to the inoculum amount of 25%, and place it on a constant temperature shaker, and carry out shaking culture at a rate of 100 r / min at 35°C After 40 hours, stand at room temperature...

Embodiment 3

[0049] Example 3 A method for preparation of jarosite by microbial transformation, comprising the following steps:

[0050] ⑴Preparation of 9K medium; ⑵Preparation of biotransformation medium; ⑶Preparation of soft agarose medium; Example 1 .

[0051] (5) Strain activation: the Leptospira ferrophilum BYQ obtained in step (4) Leptospirillum ferriphilum BYQ) strains were inoculated into shake flasks containing 9K medium with 10% inoculum amount, placed on a constant temperature shaker, and shaken at a rate of 150 r / min for 72 hours at 30°C to obtain Bacteria activation solution.

[0052] Wherein: the bottling capacity of 9K medium in the shake flask is 50%;

[0053] ⑹Biotransformation: inoculate the strain activation liquid into the shake flask containing the biotransformation medium according to the inoculum amount of 20%, and place it on a constant temperature shaker, and carry out shaking culture at a rate of 150 r / min under the condition of 30°C After 50 hours, sta...

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PUM

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Abstract

The invention relates to a method for preparing jarosite through microbial conversion. The method comprises the following steps: (1) preparing a 9K culture medium; (2) preparing a microbial conversion culture medium; (3) preparing a soft agarose culture medium; (4) performing culture preservation, namely mixing leptospirillum ferriphilum BYQ bacteria liquid in a stable phase preserved at the temperature of 0 DEG C with the soft agarose culture medium, solidifying at room temperature, and preserving at the temperature of 2-4 DEG C; (5) activating the strain, namely inoculating the leptospirillum ferriphilum BYQ strain into a shake flask containing the 9K culture medium and performing shaking culture, thus obtaining a strain activation solution; (6) performing biological conversion, namely inoculating the strain activation solution to a shake flask containing the microbial conversion culture medium and performing shaking culture, and standing at room temperature, thus obtaining jarosite which is attached to the wall of the shake flask and is generated through biological conversion; (7) soaking and centrifuging the shake flask to which the jarosite is attached by using a hydrochloric acid solution, thus obtaining a precipitate; and (8) performing vacuum drying on the precipitate to constant weight, and grinding to obtain the jarosite. The method is rapid, simple, convenient and environmentally friendly.

Description

technical field [0001] The invention relates to a method for preparing jarosite, in particular to a method for preparing jarosite through microbial conversion. Background technique [0002] Jarosite [KFe 3 (SO 4 ) 2 (0H) 6 ] is a natural mineral originating in Jaroso Canyon in Spain. It has strong hiding power, non-toxic and acid-resistant properties. It is a high-performance, rare and expensive yellow inorganic pigment. At the same time, jarosite is also widely used to remove iron in the smelting of zinc, nickel, cobalt and other metal industries. Since jarosite minerals are neither soluble in dilute acid, but also easy to precipitate, wash and filter, under certain temperature, acidity and the presence of ammonium or alkali metal ions, the Fe in the solution can be 3+ The ions form jarosite minerals and precipitate, so that the Fe ions present in the leaching solution are removed. This characteristic of jarosite makes it have important application prospects in the fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/01
Inventor 陈朋晏磊王宁波李素岳路广通
Owner LANZHOU UNIVERSITY
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