Method for rapidly screening space mutation variant strains of myxobacteria

A technology of mutant strains and spaceflight mutagenesis, applied in the biological field, can solve the problems of time-consuming, difficult to separate and purify myxobacteria, and achieve the effect of improving the screening efficiency.

Pending Publication Date: 2022-03-04
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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Problems solved by technology

However, because Myxococcus xanthus has complex multicellular cooperative behaviors, which makes the individual cells of Myxobacteria adhere to each other, it is difficult to obtain mutant strains by dilution coating like other bacteria, and the traditional method of isolating Myxobacteria is difficult and time-consuming to purify. Time

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  • Method for rapidly screening space mutation variant strains of myxobacteria
  • Method for rapidly screening space mutation variant strains of myxobacteria
  • Method for rapidly screening space mutation variant strains of myxobacteria

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Embodiment 1

[0026] This embodiment provides a method for rapidly screening Myxococcus xanthus space mutation mutant strains, comprising the following steps:

[0027] First, inoculate the returned strain of Myxococcus xanthus into CTT solid medium (medium formula: 10g casein; 1.98g MgSO 4 ·7H 2 O; 10mM pH 7.6 Tris-HCl; 1mM pH 7.6 KH 2 PO 4 / K 2 HPO 4 , add water to make the volume to 1L, adjust the pH of the medium to 7.6 with KOH, add 15g of agar, mix well, sterilize with damp heat at 121°C for 20min) and cultivate on a plate at a culture temperature of 30°C for 3 days.

[0028] Prepare screening solid medium plates. In this example, three types of screening solid medium plates are prepared, namely: CTT plate, 1wt% milk plate and Congo red plate. Among them, the CTT plate uses the CTT solid medium plate used for the above-mentioned cultivation of Myxococcus xanthus, which is used to screen the mutant strains of Myxococcus xanthomouse function loss; the 1wt% milk plate is added to the...

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Abstract

The invention discloses a method for rapidly screening space mutation variant strains of myxobacteria. The method comprises the following steps: inoculating myxobacteria subjected to space mutation to a soft agar culture medium for screening for culture, and limiting the motion behavior of the myxobacteria through soft agar, so as to screen the space mutation variant strains of the myxobacteria. Tests show that the myxococcus flavus A motion-deficient mutant is obtained by adopting the method disclosed by the invention; adding milk as an indicator to obtain a variant strain with improved milk degradation capability; a bacterial strain with bacterial colony color variation is screened by adding Congo red. By adopting the method disclosed by the invention, the screening of variant strains with excellent performance can be quickly realized, and the screening efficiency of the myxococcus flavus variant strains is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for rapidly screening mutant strains of myxobacteria. Background technique [0002] Space breeding (also known as space mutation breeding) is to send crop seeds or mutagenic materials to space on a return satellite or high-altitude balloon, use the special environmental mutagenesis effect of space to mutate the seeds, and then return to the ground to cultivate new crop varieties new breeding techniques. Myxobacteria are Gram-negative bacteria belonging to the class Delta-proteobacteria and the order Myxobacteria. It has complex synergistic behavior between cells, and it is an important model biological material for the study of bacterial cell communication and cooperation, multicellular morphogenesis, and life evolution, and myxobacteria can produce rich biologically active substances, second only to actinomycetes. Drug source microbial resources with great dev...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 朱红惠董义杰邓名荣冯广达董红红姚青
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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