32 results about "New crop" patented technology

The invention discloses a rape waterlogging-resistant gene BnA07ERF151 and an application thereof. The nucleotide sequence of the gene BnA07ERF151 is as shown in SEQ ID NO. 1. The invention also discloses a method for preparing the rape waterlogging-resistant gene BnA07ERF151. According to the invention, a brand new stain-resistant gene BnA07ERF151 is cloned from rape for the first time, and is transferred into arabidopsis thaliana to construct a pure line plant and complete stain resistance identification. Experimental results show that the growth state of a transgenic BnA07ERF151 gene strain in a waterlogging environment is remarkably superior to that of a wild type, bolting development is normal, the survival rate is increased to 43.75% from 12.5%, and the key effect of the gene on enhancing the waterlogging resistance of plants is clearly confirmed; successful cloning of the waterlogging-resistant gene provides a core gene resource for cultivation of new crop varieties, a variety of crops can be endowed with stronger waterlogging tolerance through a transgenic assisted breeding means, and the yield stability under extreme climate is effectively guaranteed, which has great strategic significance in improvement of global agricultural disaster resistance and guarantee of grain safety, and has a wide application prospect. And the method has remarkable social and economic benefits and industrial application prospects.

The invention relates to the technical field of smart agriculture, and discloses a spectrum feed-forward greenhouse control system based on physiological vector Pareto optimization, comprising an intelligent sensing module for collecting crop pulse modulation fluorescence parameters, spectrum fingerprints and environmental parameters; the model decision-making module is coupled with the three algorithm units, the long-period unit is used for outputting an optimal allowable interval of environment regulation and control based on a crop model, the short-period unit is used for predicting physiological risks and outputting dynamic regulation weights based on spectral feedforward, and the real-time unit is used for constructing physiological vectors composed of actual photochemical quantum yield and non-photochemical quenching; executing Pareto optimization under interval constraint and weight guidance, balancing light energy utilization efficiency and physiological stress, and generating an accurate control instruction; the intelligent execution module regulates and controls the environment; and the online calibration module is used for recording data to update the crop model and the prediction model online. According to the method, long-term energy efficiency planning and instantaneous physiological optimization are coordinated, and the precision, prospect and adaptive capacity of greenhouse control are improved.

The present invention belongs to the field of plant genetic engineering technology and specifically relates to a sulfonylurea herbicide-resistant rapeseed gene, protein, primer set, recombinant expression vector, engineered bacteria, and applications thereof. The sulfonylurea herbicide-resistant rapeseed gene of the present invention comprises one or more of BnALS1m1, BnALS1m2, BnALS3m1, and BnALS3m2; the nucleotide sequences of BnALS1m1, BnALS1m2, BnALS3m1, and BnALS3m2 are shown in SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, and SEQ ID NO. 4, respectively. Transgenic expression of the sulfonylurea herbicide-resistant rapeseed gene of the present invention in crops can improve the crop's tolerance and resistance to sulfonylurea herbicides, thereby assisting in the breeding of new crop varieties with high sulfonylurea herbicide resistance.

The invention discloses an intelligent crop harvesting optimization system and method, and relates to the technical field of intelligent agriculture, and the system comprises a multi-source data collection module, a multi-source data processing module, an intelligent prediction scheduling module, an abnormality diagnosis module and a transfer learning adaptation module. According to the invention, a multi-source data acquisition module is used for acquiring soil environment, weather, crop images and agricultural machine state multi-modal data in real time and uploading the data to a cloud end; the multi-source data processing module performs space-time alignment, ETL processing and feature extraction on the acquired multi-modal data, and constructs a standardized data lake and a model training environment; the intelligent prediction scheduling module predicts the crop yield and maturity, generates a harvesting task plan in combination with a hybrid scheduling algorithm and pushes the harvesting task plan to the terminal for execution; the abnormity diagnosis module monitors abnormity of equipment, personnel and land parcels in the harvesting process in real time; and the transfer learning adaptation module realizes the harvesting optimization requirement that a small amount of data is quickly adapted to new crop varieties.

The invention belongs to the technical field of gene engineering, and particularly relates to a salt-tolerant gene ThGPX4 and application thereof. The invention provides a salt-tolerant gene ThGPX4. The salt-tolerant gene ThGPX4 is used for coding an amino acid sequence as shown in SEQ ID NO: 2. The invention finds that the ThGPX4 gene can significantly improve the salt tolerance of arabidopsis thaliana after overexpression in arabidopsis thaliana, the salt-tolerant gene ThGPX4 is constructed into a plant expression vector, genetic transformation is performed through an agrobacterium tumefaciens-mediated genetic transformation method, and a new germplasm with a salt-tolerant characteristic can be effectively obtained. The method is of great significance to cultivation of new varieties of excellent crops and wide application in production.

The utility model belongs to the field of deinsectization, particularly relates to a novel agricultural crop pest and disease damage intelligent identification and early warning device, and aims to solve the problems that an existing glass screen is affected by external environment and insect impact, traces are left on the surface of the glass screen, spectral irradiation is affected, and the normal use effect is affected to a certain degree. According to the scheme, the device comprises a device body and a top cover located above the device body, supporting rods are fixedly arranged at the four corners of the top of the device body, the top ends of the supporting rods are fixedly arranged at the bottom of the top cover, a lamp tube is fixedly arranged at the bottom of the top cover, and four glass screens are arranged between the device body and the top cover. According to the insect pest early-warning device, the dead bodies of insects are collected, recognition and data analysis are conducted on the dead bodies, so that real-time insect pest early-warning information is provided, the glass screen used for collecting the insects can be rapidly and conveniently replaced through the locking and inserting mechanisms, the practicability of the device is improved, and operation of a user is facilitated.

The present invention discloses a breeding method for high-quality wheat using Taigu genic male sterile recurrent populations, belonging to the technical field of new crop variety breeding. In the process of breeding high-quality wheat using Taigu genic male sterile recurrent populations, the present invention replaces large-scale mixed pollination with directional controlled pollination. The offspring of fertile plants in the population are evaluated using agronomic traits combined with molecular marker-assisted selection, crude protein determination, gluten index, gluten content, flour quality, and tensile strength, thereby increasing the predictability and accuracy of quality trait selection and improving breeding efficiency.

The application discloses a corn mitogen-activated protein kinase kinase gene ZmMKK1 and application thereof, relates to the technical field of plant genetic engineering, and the nucleotide sequence of the gene ZmMKK1 is shown as SEQ ID NO. 1. The ZmMKK1 gene is transferred into Arabidopsis thaliana by a method of agrobacterium mediation, and the result shows that the heat resistance of the transgenic Arabidopsis thaliana is obviously improved by overexpressing the ZmMKK1 gene. The ZmMKK1 knockout corn mutant is subjected to heat treatment, and it is found that the heat resistance of the ZmMKK1 knockout corn mutant is obviously reduced, which indicates that the ZmMKK1 positively regulates the heat resistance of plants. The application will be helpful to improving the high-temperature stress resistance of plants, has important theoretical guiding significance for cultivating new crop varieties resistant to high temperature, and has great application value for agricultural production in China.

The present application relates to a kind of corn kernel type and the SNP marker of significant correlation of storage material content, dCAPS primer and its application.The present application research finds that, located ZmRRP45B The chr5.s_175413975 of 5'UTR and the chr5.s_175413829 and chr5.s_175418484 of 3'UTR of gene are the 3 most significant SNPs associated with kernel traits;The corresponding molecular marker is developed from this, and the identification of excellent haplotype, the creation of corn high yield or high quality breeding material can realize ZmRRP45B Excellent haplotype identification, corn high yield or high quality breeding material creation, and then shorten the breeding cycle of new crop varieties, reduce the breeding cost, improve the breeding efficiency.

The invention relates to the technical field of plant genetic engineering, and discloses a trifolium repens MADs-box transcription factor as well as a coding gene and application thereof. The length of an open reading frame of the cloned trifolium repens MADs-box transcription factor TrAGL80 gene is 717bp, 238 amino acids are encoded, and after the gene is heterologous overexpressed in a wild type of medicago sativa, ROS accumulation is reduced and malic acid secretion is increased through overexpression of TrAGL80, so that the tolerance of the medicago sativa to Al is improved. By regulating and controlling the expression of the trifolium repens TrAGL80 gene, a strain with high aluminum toxicity stress resistance can be obtained, the tolerance of the plant to aluminum toxicity stress is improved, and the trifolium repens TrAGL80 gene has important significance for cultivating new crop varieties suitable for acid soil cultivation.

A farmland planting method and system based on layout optimization, a medium and a product, the method comprising: acquiring environmental basic data of a target farmland and canopy morphological parameters, root morphological parameters and initial planting parameters of a crop to be planted; constructing a crop space distribution model; respectively calculating a canopy light interception potential index and a root nutrient competition index; constructing a resource coupling evaluation function based on the canopy light interception potential index and the root nutrient competition index; adjusting initial planting parameters in the crop spatial distribution model, updating the crop spatial distribution model, and iteratively calculating a function value of a resource coupling evaluation function based on the updated crop spatial distribution model to perform iterative optimization; and when a preset iteration termination requirement is met, iteration is stopped, and a planting control instruction is generated based on a target planting parameter in the target crop spatial distribution model corresponding to the maximum function value of the resource coupling evaluation function. The reasonability of spatial configuration of farmland crops can be optimized.

This invention belongs to the field of genetic engineering technology, specifically relating to an AvCHIT1 protein and its application in improving plant salt tolerance. This invention provides an AvCHIT1 protein, the amino acid sequence of which is shown in SEQ ID NO.2. The AvCHIT1 protein of this invention plays an important role in the response to abiotic stress and can improve the salt tolerance of plants. The results of the examples show that overexpression of the AvCHIT1 protein in Arabidopsis thaliana can significantly improve the seed germination rate and salt tolerance. Constructing the AvCHIT1 gene into a plant expression vector and performing genetic transformation using Agrobacterium-mediated transformation can effectively obtain new germplasm with salt tolerance characteristics. This invention is of great significance for the breeding of superior new crop varieties and their widespread application in production.

The invention relates to the technical field of crop new variety character analysis, in particular to a crop new variety character evaluation system based on big data, which realizes dynamic full-cycle tracking of crop character development by analyzing a character cycle index of a new variety in each growth stage. Traditional evaluation mostly focuses on final character expression after the end of the growth period, character formation rules and potential risks in different stages are difficult to capture, however, the scheme depends on staged evaluation logic, target sites are screened in combination with core physiological tasks of all growth stages, gene expression, phenotypic data and environmental parameter calculation indexes are integrated, and the evaluation accuracy is improved. According to the method, the character potential and the suitability of the variety in the key stages such as seedling emergence, tillering and filling can be accurately reflected, evaluation is upgraded to'whole-course image 'from'end point snapshot', an evaluation tool better fitting the crop growth rule is provided for breeders through a'dynamic tracking and accurate screening 'combined mode, and the screening process of high-quality varieties is accelerated.

The utility model discloses a novel agricultural organic fertilizer matrix which comprises a planting box and is characterized in that a liquid storage pipe used for storing liquid fertilizer is arranged in the planting box, the edge of the liquid storage pipe is fixedly connected with a sliding block, and the sliding block is matched with a notch preset in the top of the planting box; the bottom of the liquid storage pipe is provided with an instillator for slowly injecting the liquid fertilizer into the soil; according to the crop planting device, when new crops need to be planted in the planting box, a worker aligns a sliding block to a notch in the planting box and inserts a liquid storage pipe into soil, the bottom of the sliding block can push an extrusion block to move towards the interior of a sleeve block, and when the sliding block moves to the bottom of the extrusion block, a spring can push the extrusion block to move the top of the sliding block; in this way, the liquid storage pipe can be fixed in the planting box, the liquid storage pipe is fixed to the sliding block, the liquid storage pipe cannot be turned over even if a worker turns soil, and the instillator can be kept in a vertically downward state all the time.

The application discloses an anti-insect fusion gene M1 CryAb- VIP3A , an expression vector thereof, a product and application thereof, and aims to solve the technical problem that the existing Bt fusion gene is difficult to consider biological safety and insecticidal effect. M1 CryAb-VIP3A The nucleic acid sequence of the anti-insect fusion gene is shown as SEQ ID NO. 5, and the protein sequence corresponding to the nucleic acid sequence is shown as SEQ ID NO. 6. The fusion gene does not contain a sensitizer sequence and can be efficiently expressed, and the biological safety and insecticidal effect are considered, the anti-insect and insecticidal effect of the expression product is good and wide-spectrum. M1 CryAb-VIP3A M1 CryAb-VIP3A After the gene is introduced into corn, a new crop variety which is resistant to stink bugs and other lepidopteran pests or coleopteran pests (spodoptera frugiperda) is obtained; the anti-insect fusion gene expression protein M1CryAb-VIP3A has excellent insecticidal effect, can be applied to the preparation of an anti-insect preparation, is green and environment-friendly, and has no pollution.

The invention discloses application of a ZmTCP20 gene in regulation and control of crop growth, development and yield, and belongs to the technical field of gene engineering. It is found that the corn TCP family member ZmTCP20 has the effect of directly and positively regulating the nitrogen utilization efficiency of crops, nitrogen absorption of the crops can be promoted through overexpression of the ZmTCP20 gene, the biomass and total nitrogen accumulation of plants can be remarkably increased, and the nitrate absorption capacity of root systems is improved; meanwhile, two-year field trials further show that the overexpression gene ZmTCP20 can significantly improve the plant height and biomass of crops, increase the nitrogen accumulation amount in the plants, enable crop clusters to be lengthened and thickened and further improve the yield of the clusters, and after the gene ZmTCP20 is knocked out, the contrary is that the overexpression gene ZmTCP20 is knocked out. The functional analysis of the gene ZmTCP20 provides important gene resources and theoretical guidance for the cultivation of new crop varieties with efficient nitrogen utilization.

Provided is a use of a ZmGLK36 protein. The use comprises improving drought resistance of crops, promoting nitrogen use efficiency of the crops, and enhancing photosynthesis of the crops. The present invention provides new genetic resources for breeding new crop varieties having enhanced photosynthesis, drought resistance, and nitrogen use efficiency, and is of great significance for future exploration of the way to enhance the crop yield potential through genetic improvement and guarantee national food security.

The present invention discloses an Arabidopsis thaliana LncRNA44 and its applications. The nucleotide sequence of LncRNA44 is shown in SEQ ID No. 1. It was cloned from Arabidopsis thaliana and has been verified to regulate the plant's resistance to high temperatures during the germination period. By increasing the expression level of LncRNA44 in the plant, transgenic Arabidopsis thaliana with greater heat resistance than wild-type plants can be obtained. The LncRNA44 described in the present invention can provide a theoretical basis and gene source for the development of new crop varieties.

The application discloses a kind of ME15-Cas12i3-5M fusion protein and its application in rice single gene and multiple gene editing, belong to the field of rice gene editing.The technical problem solved by the present application is how to edit multiple genes.The sequence of the ME15-Cas12i3-5M fusion protein disclosed by the present application is SEQ ID No.12.The ME15-Cas12i3-5M fusion protein of the present application can not only improve the editing efficiency of single gene, but also edit multiple genes, and has good editing efficiency.The present application can be used for crop gene function research and the creation of new crop germplasm of a generation of polymerization multiple excellent alleles.

The invention discloses application of a tobacco NbASL gene in improvement of plant virus resistance, and belongs to the technical field of molecular biology. The nucleotide sequence of the NbASL gene is as shown in SEQ ID NO: 1. The NbASL gene is silenced in nicotiana benthamiana, the sensitivity of the plant to potato Y virus (PVY) and pepper vein mottle virus (ChiVMV) is obviously enhanced, and the virus accumulation amount is obviously increased. On the contrary, overexpression of the NbASL gene can significantly inhibit infection of PVY and reduce accumulation of virus RNA. And the expression of the NbASL gene in the nicotiana benthamiana can be induced to be up-regulated by PVY or ChiVMV infection. Therefore, the NbASL gene is a positive regulatory factor in antiviral reaction of plants. The invention provides important gene resources and theoretical basis for cultivating broad-spectrum and durable antiviral new crop varieties by using the NbASL gene, and has important application value.

The invention belongs to the technical field of biology, and particularly relates to a rice heading period regulation gene OsSEC and application thereof in rice heading period regulation. According to the invention, the OsSEC gene is separated and cloned from rice, and it is proved for the first time that the gene participates in rice heading period regulation and control by constructing a CRISPR / Cas9 mutant; experiments prove that the OsSEC gene in wild type rice is knocked down, and transgenic rice with an advanced heading period can be obtained. Compared with receptor rice, the OsSEC gene expression in transgenic rice is reduced. Therefore, the OsSEC gene is related to the heading period of the rice, and a theoretical basis is laid for cultivating transgenic plants for regulating and controlling the heading period in advance. The invention has important theoretical significance for further clarification of the molecular mechanism of plant heading stage regulation and control and cultivation of new crop varieties with advanced heading stages through genetic engineering means.

This invention discloses a *Styrax chinensis* inositol monophosphatase gene that can enhance plant resistance to aluminum toxicity and low phosphorus stress. SgIMP1 Its applications belong to the field of genetic engineering technology. SgIMP1 The nucleotide sequence of the gene is shown in SEQ ID NO:1. This gene has the biological function of enhancing the plant's tolerance to aluminum toxicity stress; experiments in this invention have confirmed that heterologous expression... SgIMP1 The transgenic plants containing this gene showed a significant increase in inositol content and were effectively enhanced in their tolerance to aluminum toxicity stress and low phosphorus stress. This gene can serve as an important gene for the improvement of transgenic crops, providing important scientific basis and technical support for the subsequent development of new crop germplasm suitable for cultivation in strongly acidic soils.

This utility model provides a constant temperature incubator for the cultivation of new crop varieties, relating to the field of crop cultivation technology. It includes a fixed base, a constant temperature heating module, and a constant temperature and humidity incubation mechanism. As shown in Figure 2, the drip irrigation nozzles are symmetrically positioned with crop-attaching brackets at their lower ends. During use, purified water is filled into the moisture-absorbing foam that penetrates the protective top shell. When the foam reaches a specified threshold, the moisture-absorbing foam drips water at a specified flow rate into the baffle plate and onto the surface of the crop culture medium through the lower drip irrigation nozzles, maintaining appropriate humidity. The purified water dripping through the openings at the lower end of the culture medium falls onto the surface of the constant temperature heating module for vaporization. The vaporized water vapor enters the incubator through the constant temperature grid, ensuring a relatively stable humidity level within the incubator, thus achieving the purpose of constant temperature and humidity cultivation.

This invention relates to the field of agricultural breeding technology, specifically to a method for screening nitrogen-efficient rice mutants. The method includes: providing a rice mutant population subjected to space-induced mutagenesis; subjecting the rice mutant population to hydroponic stress treatment with chlorate solution before the three-leaf stage; measuring the seedling length of the treated group and the control group, and calculating the seedling length inhibition rate; and screening nitrogen-efficient candidate mutants based on the seedling length inhibition rate. This invention also relates to nitrogen-efficient rice mutants obtained by the method and their application in nitrogen-efficient rice breeding. The method of this invention is simple to operate, has a short cycle time, and low cost, enabling rapid and accurate screening of nitrogen-efficient materials from large-scale populations, which is of great significance for cultivating new crop varieties with efficient nitrogen fertilizer utilization.

The utility model discloses a drying device for new crop variety breeding, which belongs to the field of crop breeding and comprises a drying tank, a top cover is arranged at the top of the drying tank, a feed pipe is arranged at the top of the top cover, a conical part is arranged at the bottom of the drying tank, a discharge pipe is arranged at the bottom end of the conical part, and a screen shoe is arranged in the drying tank. The screen frame is an inverted cone; by designing the inverted cone-shaped screen frame and arranging a plurality of conical screen meshes with gradually increased pore diameters in the screen frame, graded drying of seed particles with different sizes can be realized, and small-particle seeds fall to the lower layer through the upper-layer small-pore-diameter screen mesh earlier to be further dried; and large-particle seeds continue to be dried on the upper-layer screen and fall off through larger apertures until reaching a proper drying degree, so that the drying rate difference caused by different sizes of the seeds is avoided, and the whole drying process is more uniform and efficient.

The present invention relates to the technical field of genetic engineering, and in particular to a fusion gene for improving stress resistance of a plant, a construction method therefor, and a use thereof. The fusion gene is named AR1, and the nucleotide sequence thereof is as shown in SEQ NO. 1. The provided fusion gene for improving the stress resistance of a plant can significantly promote the growth of the plant while greatly improving the resistance of the plant to a high temperature and saline alkali stress, can be widely used in fields such as cultivation of new crop varieties having multi-stress resistance, and has important application value. The provided construction method for the fusion gene can successfully construct the fusion gene for improving the stress resistance of the plant. The construction method involves simple operation and easily controllable conditions, facilitating widespread promotion and use.

The invention relates to the technical field of drip irrigation control, and discloses a drip irrigation system for towel gourd cultivation, which comprises an atmosphere demand simulation unit, a soil humidity sensing unit, a drip irrigation execution unit and a main controller, the main controller takes a feedforward demand signal generated by the atmosphere demand simulation unit and a dynamically updated crop coefficient as a main basis for irrigation, and performs safety correction by using a feedback correction signal VWC generated by the soil humidity sensing unit; the main controller further utilizes the historical irrigation amount, the soil humidity net variation VWC and the time integral value of the feedforward demand signal to continuously update the crop coefficient through water balance calculation.

The application discloses a kind of fusion protein and its application in rice single gene and multiple gene editing, belong to the field of biotechnology.The technical problem solved by the application is how to edit multiple genes.The sequence of the fusion protein disclosed by the application is SEQ ID No.6, SEQ ID No.8, SEQ ID No.10 or SEQ ID No.12.The fusion protein of the application can not only improve the editing efficiency of single gene, and the editing efficiency can reach 98.61%;It can also edit multiple genes, and has good editing efficiency, and the efficiency of simultaneous editing of three genes, four genes, five genes and six genes mediated by it can reach 82.76%, 61.36%, 52.94% and 51.07%.The application can be used for crop gene function research and creation of new crop germplasm of a generation of polymerization multiple excellent alleles.

The invention discloses a lolium multiflorum LmNRAMP5-CS gene and application of the lolium multiflorum LmNRAMP5-CS gene in plant genetic improvement, and belongs to the field of crop genetic breeding. According to the present invention, the mutation lolium multiflorum LmNRAMP5 gene is specifically truncated, such that the LmNRAMP5-CS gene of which the nucleotide sequence is represented by SEQ ID NO. 1 is obtained; experimental results show that overexpression of the LmNRAMP5-CS gene can significantly promote the growth and yield increase of the plant and enhance the tolerance of the plant to cadmium toxicity and iron deficiency stress on the premise of not increasing the total cadmium accumulation amount. The LmNRAMP5-CS gene provided by the invention can be used for creating new crop germplasm which is high in yield, resistant to heavy metal stress and controllable in plant cadmium accumulation, is particularly suitable for molecular design breeding for safe and efficient production in cadmium-polluted soil, and can remarkably improve the breeding selection efficiency and accelerate the breeding process of high-quality and safe crop varieties.

The invention discloses an application of SRM1 protein and a coding gene thereof in regulation and control of plant disease resistance and growth, and belongs to the technical field of biology. The technical problem to be solved by the invention is how to regulate and control the disease resistance and / or biomass and / or growth and development of plants. The SRM1 protein disclosed by the invention is a protein of which the amino acid sequence is SEQ ID No.1, and the coding gene of the SRM1 protein is a DNA (Deoxyribose Nucleic Acid) molecule at the 800th to 1696th sites of SEQ ID No.2. Experiments prove that the SRM1 protein can simultaneously positively regulate and control plant immune response and growth and development, an SRM1 mutant plant is sensitive to pathogenic bacteria, leaf growth is inhibited, and an SRM1 overexpression strain shows higher disease resistance and remarkably enhanced vegetative growth. The SRM1 protein and the coding gene thereof provide key gene resources and accurate regulation targets for cultivating novel crop varieties with disease resistance and high yield.