42 results about "New crop" patented technology

The invention discloses a rape waterlogging-resistant gene BnA07ERF151 and an application thereof. The nucleotide sequence of the gene BnA07ERF151 is as shown in SEQ ID NO. 1. The invention also discloses a method for preparing the rape waterlogging-resistant gene BnA07ERF151. According to the invention, a brand new stain-resistant gene BnA07ERF151 is cloned from rape for the first time, and is transferred into arabidopsis thaliana to construct a pure line plant and complete stain resistance identification. Experimental results show that the growth state of a transgenic BnA07ERF151 gene strain in a waterlogging environment is remarkably superior to that of a wild type, bolting development is normal, the survival rate is increased to 43.75% from 12.5%, and the key effect of the gene on enhancing the waterlogging resistance of plants is clearly confirmed; successful cloning of the waterlogging-resistant gene provides a core gene resource for cultivation of new crop varieties, a variety of crops can be endowed with stronger waterlogging tolerance through a transgenic assisted breeding means, and the yield stability under extreme climate is effectively guaranteed, which has great strategic significance in improvement of global agricultural disaster resistance and guarantee of grain safety, and has a wide application prospect. And the method has remarkable social and economic benefits and industrial application prospects.

The utility model relates to the technical field of novel crop seed processing, and discloses a novel anti-caking corn seed coating and drying device which comprises a fixed cylinder, a plurality of heating rods are fixedly connected to the inner wall of the fixed cylinder, a rotating mechanism is arranged in the fixed cylinder, and the heating rods are fixedly connected to the inner wall of the fixed cylinder. A plurality of blow-drying mechanisms are arranged above the fixing cylinder, a control mechanism is arranged below the fixing cylinder, and the rotating mechanism comprises a sleeve. Through the sleeve connected to the middle of the left side of the fixed cylinder, the rotating shaft can rotate in the sleeve, the first motor drives the ventilation cylinder to rotate in the fixed cylinder through the rotating rod, the interior of the ventilation cylinder is used for storing corn seeds, and the first sealing disc is screwed to the right side of the ventilation cylinder through the first threaded pipe; and a second sealing disc is screwed on the right side of the fixed cylinder through a second threaded pipe to seal the interiors of the ventilating cylinder and the fixed cylinder, so that the effects of uniformly drying the seeds and preventing caking can be achieved.

The invention relates to the technical field of smart agriculture, and discloses a spectrum feed-forward greenhouse control system based on physiological vector Pareto optimization, comprising an intelligent sensing module for collecting crop pulse modulation fluorescence parameters, spectrum fingerprints and environmental parameters; the model decision-making module is coupled with the three algorithm units, the long-period unit is used for outputting an optimal allowable interval of environment regulation and control based on a crop model, the short-period unit is used for predicting physiological risks and outputting dynamic regulation weights based on spectral feedforward, and the real-time unit is used for constructing physiological vectors composed of actual photochemical quantum yield and non-photochemical quenching; executing Pareto optimization under interval constraint and weight guidance, balancing light energy utilization efficiency and physiological stress, and generating an accurate control instruction; the intelligent execution module regulates and controls the environment; and the online calibration module is used for recording data to update the crop model and the prediction model online. According to the method, long-term energy efficiency planning and instantaneous physiological optimization are coordinated, and the precision, prospect and adaptive capacity of greenhouse control are improved.

The present invention belongs to the field of plant genetic engineering technology and specifically relates to a sulfonylurea herbicide-resistant rapeseed gene, protein, primer set, recombinant expression vector, engineered bacteria, and applications thereof. The sulfonylurea herbicide-resistant rapeseed gene of the present invention comprises one or more of BnALS1m1, BnALS1m2, BnALS3m1, and BnALS3m2; the nucleotide sequences of BnALS1m1, BnALS1m2, BnALS3m1, and BnALS3m2 are shown in SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, and SEQ ID NO. 4, respectively. Transgenic expression of the sulfonylurea herbicide-resistant rapeseed gene of the present invention in crops can improve the crop's tolerance and resistance to sulfonylurea herbicides, thereby assisting in the breeding of new crop varieties with high sulfonylurea herbicide resistance.

The present invention belongs to the technical field of genetic engineering, and particularly relates to an GmHMGS application of a gene and a recombinant overexpression vector in regulating the fatty acid content of plants, and the GmHMGS nucleotide sequence of the gene is shown as SEQ ID NO.1. The present invention uses an overexpression vector of an exogenous gene sequence to introduce the GmHMGS gene shown as SEQ ID NO.1 into plants for overexpression GmHMGS of the gene, and the plants show that the contents of palmitoleic acid, arachidonic acid, docosanoic acid and erucic acid are significantly higher than those of the wild type; at the same time, the contents of stearic acid, oleic acid and linolenic acid are significantly reduced. The present invention regulates the GmHMGS expression of the gene in plants and then uses it to cultivate new plant varieties with adjustable fatty acid content, which provides a new idea for cultivating new crop varieties and has great application value in molecular breeding.

The invention relates to an SNP (Single Nucleotide Polymorphism) marker obviously associated with corn grain type and storage substance content, a dCAPS primer and application of the dCAPS primer. The research finds that chr5. S175413975 located on 5 'UTR of the ZmRRP45B gene and chr5. S175413829 and chr5. S175418484 located on 3' UTR of the ZmRRP45B gene are three most significant SNPs associated with grain traits, so that the corresponding molecular markers are developed, identification of ZmRRP45B excellent haplotypes and creation of corn high-yield or high-quality breeding materials can be realized, the breeding period of new crop varieties is shortened, the breeding cost is reduced, and the breeding efficiency is improved.

The invention discloses an intelligent crop harvesting optimization system and method, and relates to the technical field of intelligent agriculture, and the system comprises a multi-source data collection module, a multi-source data processing module, an intelligent prediction scheduling module, an abnormality diagnosis module and a transfer learning adaptation module. According to the invention, a multi-source data acquisition module is used for acquiring soil environment, weather, crop images and agricultural machine state multi-modal data in real time and uploading the data to a cloud end; the multi-source data processing module performs space-time alignment, ETL processing and feature extraction on the acquired multi-modal data, and constructs a standardized data lake and a model training environment; the intelligent prediction scheduling module predicts the crop yield and maturity, generates a harvesting task plan in combination with a hybrid scheduling algorithm and pushes the harvesting task plan to the terminal for execution; the abnormity diagnosis module monitors abnormity of equipment, personnel and land parcels in the harvesting process in real time; and the transfer learning adaptation module realizes the harvesting optimization requirement that a small amount of data is quickly adapted to new crop varieties.

The invention belongs to the technical field of gene engineering, and particularly relates to a salt-tolerant gene ThGPX4 and application thereof. The invention provides a salt-tolerant gene ThGPX4. The salt-tolerant gene ThGPX4 is used for coding an amino acid sequence as shown in SEQ ID NO: 2. The invention finds that the ThGPX4 gene can significantly improve the salt tolerance of arabidopsis thaliana after overexpression in arabidopsis thaliana, the salt-tolerant gene ThGPX4 is constructed into a plant expression vector, genetic transformation is performed through an agrobacterium tumefaciens-mediated genetic transformation method, and a new germplasm with a salt-tolerant characteristic can be effectively obtained. The method is of great significance to cultivation of new varieties of excellent crops and wide application in production.

The utility model belongs to the field of deinsectization, particularly relates to a novel agricultural crop pest and disease damage intelligent identification and early warning device, and aims to solve the problems that an existing glass screen is affected by external environment and insect impact, traces are left on the surface of the glass screen, spectral irradiation is affected, and the normal use effect is affected to a certain degree. According to the scheme, the device comprises a device body and a top cover located above the device body, supporting rods are fixedly arranged at the four corners of the top of the device body, the top ends of the supporting rods are fixedly arranged at the bottom of the top cover, a lamp tube is fixedly arranged at the bottom of the top cover, and four glass screens are arranged between the device body and the top cover. According to the insect pest early-warning device, the dead bodies of insects are collected, recognition and data analysis are conducted on the dead bodies, so that real-time insect pest early-warning information is provided, the glass screen used for collecting the insects can be rapidly and conveniently replaced through the locking and inserting mechanisms, the practicability of the device is improved, and operation of a user is facilitated.

The present invention discloses a breeding method for high-quality wheat using Taigu genic male sterile recurrent populations, belonging to the technical field of new crop variety breeding. In the process of breeding high-quality wheat using Taigu genic male sterile recurrent populations, the present invention replaces large-scale mixed pollination with directional controlled pollination. The offspring of fertile plants in the population are evaluated using agronomic traits combined with molecular marker-assisted selection, crude protein determination, gluten index, gluten content, flour quality, and tensile strength, thereby increasing the predictability and accuracy of quality trait selection and improving breeding efficiency.

The application discloses a corn mitogen-activated protein kinase kinase gene ZmMKK1 and application thereof, relates to the technical field of plant genetic engineering, and the nucleotide sequence of the gene ZmMKK1 is shown as SEQ ID NO. 1. The ZmMKK1 gene is transferred into Arabidopsis thaliana by a method of agrobacterium mediation, and the result shows that the heat resistance of the transgenic Arabidopsis thaliana is obviously improved by overexpressing the ZmMKK1 gene. The ZmMKK1 knockout corn mutant is subjected to heat treatment, and it is found that the heat resistance of the ZmMKK1 knockout corn mutant is obviously reduced, which indicates that the ZmMKK1 positively regulates the heat resistance of plants. The application will be helpful to improving the high-temperature stress resistance of plants, has important theoretical guiding significance for cultivating new crop varieties resistant to high temperature, and has great application value for agricultural production in China.

The present application relates to a kind of corn kernel type and the SNP marker of significant correlation of storage material content, dCAPS primer and its application.The present application research finds that, located ZmRRP45B The chr5.s_175413975 of 5'UTR and the chr5.s_175413829 and chr5.s_175418484 of 3'UTR of gene are the 3 most significant SNPs associated with kernel traits;The corresponding molecular marker is developed from this, and the identification of excellent haplotype, the creation of corn high yield or high quality breeding material can realize ZmRRP45B Excellent haplotype identification, corn high yield or high quality breeding material creation, and then shorten the breeding cycle of new crop varieties, reduce the breeding cost, improve the breeding efficiency.

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The invention relates to the technical field of plant genetic engineering, and discloses a trifolium repens MADs-box transcription factor as well as a coding gene and application thereof. The length of an open reading frame of the cloned trifolium repens MADs-box transcription factor TrAGL80 gene is 717bp, 238 amino acids are encoded, and after the gene is heterologous overexpressed in a wild type of medicago sativa, ROS accumulation is reduced and malic acid secretion is increased through overexpression of TrAGL80, so that the tolerance of the medicago sativa to Al is improved. By regulating and controlling the expression of the trifolium repens TrAGL80 gene, a strain with high aluminum toxicity stress resistance can be obtained, the tolerance of the plant to aluminum toxicity stress is improved, and the trifolium repens TrAGL80 gene has important significance for cultivating new crop varieties suitable for acid soil cultivation.

A farmland planting method and system based on layout optimization, a medium and a product, the method comprising: acquiring environmental basic data of a target farmland and canopy morphological parameters, root morphological parameters and initial planting parameters of a crop to be planted; constructing a crop space distribution model; respectively calculating a canopy light interception potential index and a root nutrient competition index; constructing a resource coupling evaluation function based on the canopy light interception potential index and the root nutrient competition index; adjusting initial planting parameters in the crop spatial distribution model, updating the crop spatial distribution model, and iteratively calculating a function value of a resource coupling evaluation function based on the updated crop spatial distribution model to perform iterative optimization; and when a preset iteration termination requirement is met, iteration is stopped, and a planting control instruction is generated based on a target planting parameter in the target crop spatial distribution model corresponding to the maximum function value of the resource coupling evaluation function. The reasonability of spatial configuration of farmland crops can be optimized.

The present invention provides a wheat TabZIP19-5B gene and its application in regulating wheat salt tolerance, antiviral ability and thousand-grain weight, belonging to the technical field of plant genetic engineering. By using the existing plant genetic engineering technology, the present invention for the first time clones the gene TabZIP19-5B that regulates wheat salt tolerance, antiviral ability and thousand-grain weight, and transfers this gene into wheat by an Agrobacterium-mediated method. Through comparative test analysis, it is proved that the transgenic plants overexpressing TabZIP19-5B of the present invention have significantly improved salt tolerance and resistance to BYDV, and the grain width is greater than that of the wild type. The TabZIP19-5B gene provided by the present invention plays an important role in regulating salt tolerance, resistance to BYDV and thousand-grain weight, and can be used as an important gene resource for cultivating new crop varieties with salt tolerance, antiviral ability and high thousand-grain weight.

This invention belongs to the field of genetic engineering technology, specifically relating to an AvCHIT1 protein and its application in improving plant salt tolerance. This invention provides an AvCHIT1 protein, the amino acid sequence of which is shown in SEQ ID NO.2. The AvCHIT1 protein of this invention plays an important role in the response to abiotic stress and can improve the salt tolerance of plants. The results of the examples show that overexpression of the AvCHIT1 protein in Arabidopsis thaliana can significantly improve the seed germination rate and salt tolerance. Constructing the AvCHIT1 gene into a plant expression vector and performing genetic transformation using Agrobacterium-mediated transformation can effectively obtain new germplasm with salt tolerance characteristics. This invention is of great significance for the breeding of superior new crop varieties and their widespread application in production.

In particular, the present invention relates to a crop nutrition and fortifying composition comprising: elemental sulfur accounting for 1% to 90% of the total weight of the composition; at least one micronutrient selected from selenium and vanadium in elemental form, or salts, complexes or derivatives thereof, where the content of the element selenium or vanadium accounts for 0.001% to 10% of the total weight of the composition; and at least one nonionic or anionic surfactant; wherein the composition comprises microparticles having a size of 0.1 [mu] m to 30 [mu] m, and the composition is a water dispersible granule or an aqueous suspension. The invention also relates to a preparation method of the crop nutrition and fortifying composition. The present invention also relates to a method of enhancing nutrient uptake or improving plant health and yield by treating a plant, plant propagation material, a locus or parts thereof, seeds, seedlings or surrounding soil with a crop nutrition and fortifying composition.

The invention relates to the technical field of crop new variety character analysis, in particular to a crop new variety character evaluation system based on big data, which realizes dynamic full-cycle tracking of crop character development by analyzing a character cycle index of a new variety in each growth stage. Traditional evaluation mostly focuses on final character expression after the end of the growth period, character formation rules and potential risks in different stages are difficult to capture, however, the scheme depends on staged evaluation logic, target sites are screened in combination with core physiological tasks of all growth stages, gene expression, phenotypic data and environmental parameter calculation indexes are integrated, and the evaluation accuracy is improved. According to the method, the character potential and the suitability of the variety in the key stages such as seedling emergence, tillering and filling can be accurately reflected, evaluation is upgraded to'whole-course image 'from'end point snapshot', an evaluation tool better fitting the crop growth rule is provided for breeders through a'dynamic tracking and accurate screening 'combined mode, and the screening process of high-quality varieties is accelerated.

The utility model discloses a novel agricultural organic fertilizer matrix which comprises a planting box and is characterized in that a liquid storage pipe used for storing liquid fertilizer is arranged in the planting box, the edge of the liquid storage pipe is fixedly connected with a sliding block, and the sliding block is matched with a notch preset in the top of the planting box; the bottom of the liquid storage pipe is provided with an instillator for slowly injecting the liquid fertilizer into the soil; according to the crop planting device, when new crops need to be planted in the planting box, a worker aligns a sliding block to a notch in the planting box and inserts a liquid storage pipe into soil, the bottom of the sliding block can push an extrusion block to move towards the interior of a sleeve block, and when the sliding block moves to the bottom of the extrusion block, a spring can push the extrusion block to move the top of the sliding block; in this way, the liquid storage pipe can be fixed in the planting box, the liquid storage pipe is fixed to the sliding block, the liquid storage pipe cannot be turned over even if a worker turns soil, and the instillator can be kept in a vertically downward state all the time.

The application discloses an anti-insect fusion gene M1 CryAb- VIP3A , an expression vector thereof, a product and application thereof, and aims to solve the technical problem that the existing Bt fusion gene is difficult to consider biological safety and insecticidal effect. M1 CryAb-VIP3A The nucleic acid sequence of the anti-insect fusion gene is shown as SEQ ID NO. 5, and the protein sequence corresponding to the nucleic acid sequence is shown as SEQ ID NO. 6. The fusion gene does not contain a sensitizer sequence and can be efficiently expressed, and the biological safety and insecticidal effect are considered, the anti-insect and insecticidal effect of the expression product is good and wide-spectrum. M1 CryAb-VIP3A M1 CryAb-VIP3A After the gene is introduced into corn, a new crop variety which is resistant to stink bugs and other lepidopteran pests or coleopteran pests (spodoptera frugiperda) is obtained; the anti-insect fusion gene expression protein M1CryAb-VIP3A has excellent insecticidal effect, can be applied to the preparation of an anti-insect preparation, is green and environment-friendly, and has no pollution.

The invention discloses application of a ZmTCP20 gene in regulation and control of crop growth, development and yield, and belongs to the technical field of gene engineering. It is found that the corn TCP family member ZmTCP20 has the effect of directly and positively regulating the nitrogen utilization efficiency of crops, nitrogen absorption of the crops can be promoted through overexpression of the ZmTCP20 gene, the biomass and total nitrogen accumulation of plants can be remarkably increased, and the nitrate absorption capacity of root systems is improved; meanwhile, two-year field trials further show that the overexpression gene ZmTCP20 can significantly improve the plant height and biomass of crops, increase the nitrogen accumulation amount in the plants, enable crop clusters to be lengthened and thickened and further improve the yield of the clusters, and after the gene ZmTCP20 is knocked out, the contrary is that the overexpression gene ZmTCP20 is knocked out. The functional analysis of the gene ZmTCP20 provides important gene resources and theoretical guidance for the cultivation of new crop varieties with efficient nitrogen utilization.

Provided is a use of a ZmGLK36 protein. The use comprises improving drought resistance of crops, promoting nitrogen use efficiency of the crops, and enhancing photosynthesis of the crops. The present invention provides new genetic resources for breeding new crop varieties having enhanced photosynthesis, drought resistance, and nitrogen use efficiency, and is of great significance for future exploration of the way to enhance the crop yield potential through genetic improvement and guarantee national food security.

The present invention discloses an Arabidopsis thaliana LncRNA44 and its applications. The nucleotide sequence of LncRNA44 is shown in SEQ ID No. 1. It was cloned from Arabidopsis thaliana and has been verified to regulate the plant's resistance to high temperatures during the germination period. By increasing the expression level of LncRNA44 in the plant, transgenic Arabidopsis thaliana with greater heat resistance than wild-type plants can be obtained. The LncRNA44 described in the present invention can provide a theoretical basis and gene source for the development of new crop varieties.

The application discloses a kind of ME15-Cas12i3-5M fusion protein and its application in rice single gene and multiple gene editing, belong to the field of rice gene editing.The technical problem solved by the present application is how to edit multiple genes.The sequence of the ME15-Cas12i3-5M fusion protein disclosed by the present application is SEQ ID No.12.The ME15-Cas12i3-5M fusion protein of the present application can not only improve the editing efficiency of single gene, but also edit multiple genes, and has good editing efficiency.The present application can be used for crop gene function research and the creation of new crop germplasm of a generation of polymerization multiple excellent alleles.

The invention discloses application of a tobacco NbASL gene in improvement of plant virus resistance, and belongs to the technical field of molecular biology. The nucleotide sequence of the NbASL gene is as shown in SEQ ID NO: 1. The NbASL gene is silenced in nicotiana benthamiana, the sensitivity of the plant to potato Y virus (PVY) and pepper vein mottle virus (ChiVMV) is obviously enhanced, and the virus accumulation amount is obviously increased. On the contrary, overexpression of the NbASL gene can significantly inhibit infection of PVY and reduce accumulation of virus RNA. And the expression of the NbASL gene in the nicotiana benthamiana can be induced to be up-regulated by PVY or ChiVMV infection. Therefore, the NbASL gene is a positive regulatory factor in antiviral reaction of plants. The invention provides important gene resources and theoretical basis for cultivating broad-spectrum and durable antiviral new crop varieties by using the NbASL gene, and has important application value.

The invention discloses an application of a wheat receptor-like protein kinase TaHDRLK-1D1 gene in regulation and control of a heading stage of a plant. Belongs to the technical field of biology. The amino acid sequence of the TaHDRLK-1D1 protein disclosed by the invention is as shown in SEQ ID No. 3. Experiments prove that an overexpression strain of the TaHDRLK-1D1 coding gene can delay the heading stage of wheat; the TaHDRLK-1D1 coding gene is knocked out by using a CRISPR / Cas9 system, so that the heading period of wheat can be advanced. Candidate genes are provided for cultivating new crop varieties adapting to different ecological environments by excavating key genes for regulating and controlling the heading stage of wheat, and meanwhile, the gene has important theoretical and practical significance for knowing the heading stage of crops and forming related yield traits.

The invention belongs to the technical field of biology, and particularly relates to a rice heading period regulation gene OsSEC and application thereof in rice heading period regulation. According to the invention, the OsSEC gene is separated and cloned from rice, and it is proved for the first time that the gene participates in rice heading period regulation and control by constructing a CRISPR / Cas9 mutant; experiments prove that the OsSEC gene in wild type rice is knocked down, and transgenic rice with an advanced heading period can be obtained. Compared with receptor rice, the OsSEC gene expression in transgenic rice is reduced. Therefore, the OsSEC gene is related to the heading period of the rice, and a theoretical basis is laid for cultivating transgenic plants for regulating and controlling the heading period in advance. The invention has important theoretical significance for further clarification of the molecular mechanism of plant heading stage regulation and control and cultivation of new crop varieties with advanced heading stages through genetic engineering means.

The invention relates to a corn ZmRRP45B gene mutation SNP marker, a dCAPS primer and application of the corn ZmRRP45B gene mutation SNP marker and the dCAPS primer. Research finds that on the basis of a fourth version of a corn B73 reference genome, a G deletion exists at a chr5. S175415208 position, so that mutant grains become smaller, endosperm is not filled fully, the top collapses, and the hundred-grain weight is reduced; corresponding molecular markers are developed, development of corn embryos and endosperm can be regulated and controlled, high-yield or high-quality breeding materials of corn can be conveniently created, the breeding period of new crop varieties is shortened, the breeding cost is reduced, and the breeding efficiency is improved.

This invention discloses a *Styrax chinensis* inositol monophosphatase gene that can enhance plant resistance to aluminum toxicity and low phosphorus stress. SgIMP1 Its applications belong to the field of genetic engineering technology. SgIMP1 The nucleotide sequence of the gene is shown in SEQ ID NO:1. This gene has the biological function of enhancing the plant's tolerance to aluminum toxicity stress; experiments in this invention have confirmed that heterologous expression... SgIMP1 The transgenic plants containing this gene showed a significant increase in inositol content and were effectively enhanced in their tolerance to aluminum toxicity stress and low phosphorus stress. This gene can serve as an important gene for the improvement of transgenic crops, providing important scientific basis and technical support for the subsequent development of new crop germplasm suitable for cultivation in strongly acidic soils.