14 results about "Cholesterol metabolism" patented technology

Provided herein are compositions, methods of making the same, and methods for targeted delivery of therapeutic agents for modifying expression and function of target genes, e.g. proteins involved in lipid and cholesterol metabolism such as PCSK9.

The application relates to the field of biological medicine, in particular to application of TDF in serum cholesterol regulation and a verification method. The application discloses that tenofovir disoproxil fumarate (TDF) can inhibit intestinal cholesterol absorption by specifically down-regulating the expression level of NPC1L1 protein in the intestinal tract, so as to reduce serum cholesterol. The regulation has tissue specificity, and TDF has no significant influence on the expression of key genes of liver cholesterol metabolism. The application also provides a method for verifying the regulation of TDF on serum cholesterol, which is evaluated by detecting blood lipid indexes and the gene and / or protein expression level of NPC1L1 in duodenal tissue. The application provides a treatment scheme with antiviral and cholesterol-lowering effects for patients with chronic hepatitis B combined with hypercholesterolemia, and provides a new idea for developing a lipid-lowering strategy targeting intestinal cholesterol absorption.

The invention relates to the technical field of biological medicine, and particularly discloses application of nefenavir. Nefinavir is used for preparing a medicine for treating or preventing castration-resistant prostate cancer, and the medicine can specifically up-regulate expression of DHCR7, induce DNA damage of tumor cells and activate a cell cycle arrest pathway mediated by periodic protein of the cells so as to inhibit key biological processes such as proliferation of the tumor cells, cholesterol metabolism and the like; especially, formation and amplification of multinuclear giant cells in castration-resistant prostate cancer can be effectively inhibited by up-regulating expression of DHCR7, so that malignant progression of multinuclear giant cell mediated tumors and treatment of drug resistance are blocked. Finally, the castration-resistant prostate cancer is prevented and treated, and the technical bottlenecks that an existing castration-resistant prostate cancer treatment means is limited in curative effect, and the drug resistance and metastasis capability mediated by multinuclear giant cells are difficult to inhibit in a targeted manner are solved.

This application discloses the application of PLAC8 as a target in the preparation of tumor therapeutic drugs, involving the field of biomedical technology. This application clarifies that the "sympathetic nervous system-β2-AR-PLAC8-cholesterol metabolism-M2 polarization" pathway is the core driving pathway for stress-related tumor progression and chemotherapy resistance. It reveals the molecular mechanism by which PLAC8 inhibits cholesterol synthesis through phosphorylation at the S67 site, binding to the N-terminal domain of SREBP2, recruiting OGT to mediate SREBP2O-GlcNAc glycosylation. Through strategies such as gene silencing, β2-AR antagonist intervention, and targeted delivery of siPLAC8 via mannose-modified lipid nanoparticles (LNPs), the application achieved the effects of inhibiting M2 macrophage accumulation, inhibiting tumor growth, and reversing chemotherapy resistance in various tumor models, including gallbladder cancer, intrahepatic cholangiocarcinoma, and prostate cancer. Furthermore, the LNPs delivery system showed no significant toxicity. This research provides a novel therapeutic target centered on PLAC8 and a safe and effective targeted intervention strategy for stress-related tumors, highlighting its clinical translational value.

The invention discloses a sound-sensitive bionic nano-drug and a preparation method thereof, and belongs to the technical field of biological medicines. The sound-sensitive bionic nano-drug comprises porphyrin-hybridized hollow mesoporous organic silicon dioxide, a macrophage membrane and a cholesterol metabolism regulating drug, the porphyrin-hybridized hollow mesoporous organic silicon dioxide is a sound-sensitive agent and a carrier, the surface of the outer layer of the hollow mesoporous organic silicon dioxide is coated with the macrophage membrane, and the cholesterol metabolism regulating drug is coated with the macrophage membrane. Tetra (4-carboxyphenyl) porphyrin (TCPP) in a sound-sensitive agent is hybridized on a hollow mesoporous organic silicon dioxide skeleton, Ava is loaded on mesoporous and hollow structures of the TCPP, and the tumor killing effect is improved by regulating cholesterol metabolism of tumor cells and T cells and cooperating with SDT. And the sound-sensitive bionic nano-drug provides a new normal form for tumor treatment through'metabolism-sonodynamic-immunization 'multi-dimensional combination, and breaks through the limitation of traditional single treatment.

This invention belongs to the fields of biomedical materials and colloid chemistry, and discloses a structurally remodeled egg yolk low-density lipoprotein biomimetic liposome and its application in regulating cholesterol metabolism. The method includes extracting and purifying natural egg yolk low-density lipoprotein; selectively removing endogenous cholesterol by adding β-cyclodextrin under alkaline conditions; adding a phytosterol ethanol solution to the cholesterol-removed low-density lipoprotein dispersion, followed by incubation and dialysis purification to obtain the biomimetic liposome. The obtained biomimetic liposome uses partially cholesterol-removed egg yolk low-density lipoprotein as its structural framework, with phytosterols embedded in its phospholipid monolayer and lipid core interface. This biomimetic liposome can inhibit cholesterol micelle formation, reduce cholesterol absorption, promote macrophage cholesterol efflux, and inhibit macrophage foam formation. It can be used to prepare cholesterol metabolism regulating materials, functional delivery systems, and dietary supplement products.

The invention discloses a compound lactic acid bacteria composition and application thereof, and belongs to the technical field of microbial medicine. The compound lactic acid bacteria composition is prepared from lactobacillus plantarum (Lactobacillus plantarum) HMPM1006C and lactobacillus curvatus (Lactobacillus curvatus) HMPM1403C, and the ratio of the viable count of the lactobacillus plantarum (Lactobacillus plantarum) HMPM1006C to the viable count of the lactobacillus curvatus (Lactobacillus curvatus) HMPM1403C to the viable count of the lactobacillus plantarum (Lactobacillus plantarum) HMPM1403C is 1 to (0.1 to 3). Through the synergistic effect of multiple target points, absorption of carbohydrates in the intestinal tract can be delayed, insulin sensitivity can be improved, cholesterol metabolism can be promoted, meanwhile, intestinal flora balance is adjusted, inflammatory response is relieved, improvement on type 2 diabetes mellitus and related metabolic diseases is achieved, and the effect is remarkably superior to that of a single strain; a safe and effective new strategy is provided for prevention and adjuvant treatment of metabolic syndrome, type 2 diabetes and obesity.

This invention belongs to the field of pharmaceutical technology, and particularly relates to a silicon-based preparation for regulating intestinal flora to improve cholesterol metabolism and its preparation method. The method includes: S1, providing pharmaceutical-grade water-soluble silica raw material; S2, diluting the water-soluble silica raw material with sterile deionized water, stirring at 100-200 r / min for 10-20 min at 20-25°C, and preparing low-dose and high-dose silicon-based preparations respectively. This invention addresses the shortcomings of existing cholesterol-lowering drugs, such as muscle toxicity and new-onset diabetes, caused by long-term use, by providing a silicon-based preparation using pharmaceutical-grade water-soluble silica as a raw material. By limiting the content of metasilicic acid and silica in the raw material, controlling the heavy metal impurity content to ≤10 ppm, and combining sterile deionized water dilution with precise stirring conditions and pH control, the high purity and stability of the preparation are ensured.

The invention provides an application of PLAC8 as a detection target in preparation of a tumor diagnosis product, and relates to the technical field of biomedicine, the technical key points are as follows: the application of the PLAC8 as the detection target in preparation of the tumor diagnosis product and a tumor prognosis condition inspection product integrates three gallbladder cancer clinical queues, and has the advantages of high accuracy, high sensitivity and high reliability. By combining a cross-species model and technologies of multiple immunofluorescence, single cell RNA sequencing and the like, the PLAC8 is found to be a cross-species conservative M2 macrophage gene with significant difference. PLAC8 is abnormally enriched in gallbladder cancer tissues, and normal gallbladder epithelium is almost not expressed and can be stably detected through tumor tissues and peripheral blood. Expression of the gene is limited to a macrophage cluster, the gene is in positive correlation with M2 markers CD206 and CD163, and M2 polarization is promoted by regulating cholesterol metabolism. Clinical verifications show that increase of PLAC8 + CD163 + macrophages in tumors is an independent prognosis factor, high expression is closely related to short lifetime of patients and increase of chemotherapy non-response rate, and PLAC8 can be used as a potential biomarker for evaluating drug resistance of gallbladder cancer chemotherapy and tumor progression.

The invention discloses a germinated brown rice extract capable of remarkably improving cholesterol metabolism of liver as well as a preparation method and application of the germinated brown rice extract. The method comprises the following steps: soaking cleaned and disinfected brown rice in an inositol aqueous solution, and spraying the inositol aqueous solution with the same concentration during germination. And after germination is finished, drying and crushing the brown rice, adding a methanol aqueous solution, adding ascorbic acid with a certain concentration, performing ultrasonic extraction, centrifuging, and taking supernate to obtain a target extract. Compared with a conventional germinated brown rice alcohol extract, the prepared germinated brown rice extract has the advantages that the polyphenol enrichment effect is better, the polyphenol retention rate in the ultrasonic extraction process is higher, and the prepared extract can remarkably regulate liver cholesterol metabolism.

The invention discloses a production method and application of a bile acid and Chinese herbal medicine combined feed additive. The additive is prepared from the following raw materials in parts by weight: 30-50 parts of rhizoma alismatis, 15-25 parts of hawthorn, 10-20 parts of radix bupleuri, 5-15 parts of an artemisia apiacea extract, 2-8 parts of berberine, 5-15 parts of pericarpium citri reticulatae, 10-20 parts of poria cocos and 5-10 parts of liquorice. Based on the compatibility theory of'monarch, minister, assistant and guide 'of traditional Chinese medicine, various Chinese herbal medicine active ingredients such as rhizoma alismatis, hawthorn, radix bupleuri, artemisia apiacea extract, berberine, pericarpium citri reticulatae, poria cocos and liquorice are selected and scientifically formulated, all the ingredients are clear in division of labor and have a synergistic effect, the rhizoma alismatis serves as the monarch, and cholesterol metabolism is directly regulated and controlled; multi-target synergistic interaction of promoting bile acid synthesis, inhibiting inflammatory response, remodeling intestinal microecology, repairing intestinal liver circulation steady state and the like is realized, the action mechanism is clear, and the effect is superior to that of a single component or a simple mixture.

The invention discloses application of CH25H in treating intestinal cancer, and belongs to the field of disease treatment. The invention focuses on cholesterol metabolite 25-hydroxycholesterol (25HC), and aims to systematically clarify the key action mechanism of the cholesterol metabolite in macrophage metabolism reprogramming, TME remodeling and immunotherapy sensitization. The research not only clings to the leading edge of international tumor immunometabolism, but also provides a clear transformation direction for breaking through the bottleneck of colorectal cancer immunotherapy.

The invention provides an application of a dandelion water extract in preparation of a composition for improving muscle mass and skin moisture content, the dandelion water extract is obtained by extracting dandelion with a solvent, and the solvent is water. The dandelion water extract is further used for reducing the expression quantity of PPARG2 gene, C / EBP-alpha gene and GLUT4 gene and increasing the expression quantity of UCP1 gene and CETP gene so as to achieve the effects of reducing fat accumulation, promoting cholesterol metabolism, increasing muscle mass and promoting aquaporin generation.

Methods and uses of compositions (e.g., comprising one or more microbial strains, one or more components (e.g., microbial components), one or more metabolites (e.g., microbial metabolites) or any combination thereof) for preventing metabolic (e.g., lipid and cholesterol metabolism) related diseases, conditions and disorders, reducing the risk of said metabolic related diseases, conditions and disorders, treating and / or improving said metabolic related diseases, conditions and disorders are disclosed.