468 results about "Cell morphology" patented technology

A method for screening individuals at risk for prostate cancer progression is disclosed. The method is useful for evaluating cells from patients at risk for recurrence of prostate cancer following surgery for prostate cancer. Specifically, the method uses specific Markovian nuclear texture factors, alone or in combination with other biomarkers, to determine whether the cancer will progress or lose organ confinement. In addition, methods of predicting the development of fatal metastatic disease by statistical analysis of selected biomarkers is also disclosed. The invention also contemplates a method that uses a neural network to analyze and interpret cell morphology data. Utilizing Markovian factors and other biomarkers as parameters, the network is first trained with a sets of cell data from known progressors and known non-progressors. The trained network is then used to predict prostate cancer progression in patient samples.

This invention provides a method for deriving precursors to pluripotent non-embryonic stem (P-PNES) and pluripotent non-embryonic stem (PNES) cell lines. The present invention involves nuclear transfer of genetic material from a somatic cell into an enucleated, zona pellucida free human ooplastoid having a reduced amount of total cytoplasm. The present invention provides a new source for obtaining human and other animal pluripotent stem cells. The source utilizes as starting materials an oocyte and a somatic cell as the starting materials but does not require the use, creation and/or destruction of embryos or fetal tissue and does not in any way involve creating a cloned being. The oocyte never becomes fertilized and never develops into an embryo. Rather, portions of the oocyte cytoplasm are extracted and combined with the nuclear material of individual mature somatic cells in a manner that precludes embryo formation. Murine, bovine, and human examples of the procedure are demonstrated. Subsequently, the newly constructed P-PNES cells are cultured in vitro and give rise to PNES cells and cell colonies. Methods are described for culturing the P-PNES cells to yield purified PNES cells which have the ability to differentiate into cells derived from mesoderm, endoderm, and ectoderm germ layers. Methods are described for maintaining and proliferating PNES cells in culture in an undifferentiated state. Methods and results are described for analysis and validation of pluripotency of PNES cells including cell morphology, cell surface markers, pluripotent tumor development in SCID mouse, karyotyping, immortality in in vitro culture.

A method for modifying silk polymer by coupling a chemical moiety to a tyrosine residue of a silk polymer is described herein for the purpose of altering the physical properties of the silk protein. Thus, silk proteins with desired physical properties can be produced by the methods described herein. These methods are particularly useful when the introduction of cells to a mammal is desired, since modifications to the silk protein affect the physical properties and thus the adhesion, metabolic activity and cell morphology of the desired cells. The silk protein can be modified to produce, or modify, a structure that provides an optimal environment for the desired cells.

The invention provides methods of detecting cancerous cells in biological samples using a double staining/dual imaging approach, which can be used to diagnose cancer. More specifically, the present invention provides methods of diagnosing bladder cancer by a simultaneous scanning of cell morphology and FISH signals of cells derived from a urine sample.

The invention relates to a method for displaying a virtual slide capable of storing positional information of a prescribed cell in a virtual slide photographed with a magnification capable of recognizing cell morphology and a terminal device for displaying the virtual slide; or a method for displaying the virtual slide capable of classifying the prescribed cell in the virtual slide photographed with a magnification capable of recognizing cell morphology by a simple operation; or a terminal device for displaying the virtual slide.

A process for making closed-cell, alkenyl aromatic polymer foams using nano-particle nucleation agents to control the cell morphology of the resulting foam includes forming a polymer melt at a temperature above the polymer glass transition temperature (for crystal polymers) or the polymer melt point (for amorphous polymers); incorporating selected nano-particles into the polymer melt; incorporating blowing agents into the polymer melt at an elevated pressure; optionally incorporating other additives, such as flame retardants, into the polymer melt; and extruding the polymer melt under conditions sufficient to produce a foam product having a desired cell morphology, characterized by parameters such as reduced average cell size range and/or increased asymmetry of the cells.

The invention provides a classification method and a classification system for cancer digital pathological cell images. According to the classification method and the classification system, a suspected lesion region of interest is subjected to block processing, the suspected lesion region after block processing is subjected to feature extraction by utilizing partial matching pattern textural features, and the extracted features are classified and identified by adopting an extreme learning machine training method, so as to determine benign and malignant tumors and differentiate levels. The classification method and the classification system for the cancer digital pathological cell images utilize the partial matching pattern textural features for conducting feature extraction, analyze the textural features of cells from macroscopic and microscopic aspects, have the advantage of rotation invariance, effectively overcome the problems of diversity, irregularity and the like of cell morphology, provide reliable textural feature information for classification, apply an extreme learning machine to the classification of breast cancer cells, shorten the training time, accelerate the speed of classification and identification, and improve the accuracy of recognition.

The invention relates to an improved mesenchyme stem cell protection solution as well as application and a preparation method thereof. The protection solution can effectively prolong the activity remaining time of mesenchyme stem cells, reduces preparation cost, and has the advantages of wide raw material source, simple preparation, safe and reliable direct clinical application; and after the mesenchyme stem cells are preserved for 48 hours in the protection solution, the cell activity is still above 90 percent, the cell morphology is normal, and the multiplication capacity and mesenchyme stem cell phenotype characteristics are not influenced.

The invention provides a high content image flow biological microscopic analysis system. The system comprises a liquid flow system and an optical system, wherein the liquid flow system enables cells in sample cell suspension to be focused in the center of a liquid flow under the constraint of the system sheath liquid flow and to flow through a detection window of a flow chamber one by one; the optical system comprises optical sources, an optical path system and an optical filter stack; the optical sources include a halogen lamp and a laser; through the optical path system and the optical filter stack formed by a plurality of dichroscopes, the optical system generates a bright field cell image, a six-channel fluorescent cell image and six PMT (photomultiplier tube) optical intensity signals with different wave bands. The high content image flow biological microscopic analysis system integrates flow cytometry and fluorescence microscopic imaging, has a plurality of detection channels, can collect the cell images of the cells passing through the flow chamber one by one and can carry out quantitative analysis on each cell image by utilizing analysis software, thus providing the statistical data of a cell group and the information of the cell morphology, cell structures and subcellular signal distribution.

The invention provides an automatic blood cell recognition device which comprises a laser light source part, a microscope acquisition part and an objective table part which are respectively connected with a computer. The blood cells can be automatically recognized without staining or utilizing an antibody/antigen reaction on the cell surface, and the blood cell recognition is realized by utilizing cell morphology and spectral characteristic change according to a hyperspectral image. The system is a human blood cell recognition technology with the technical characteristic of automatic non-destructiveness.

Three kinds of tissues including cancer tissue, precancerosis and corresponding normal tissue are sliced up, dyed, marked, and positioned. Receptor holes are prepared by leading designed lattice array mould paper to paste on surface of wax block of receptor. Wax block with tissue core bar is prepared by using perforating needle and puncture needle for tissue. Common cancer such as lung cancer, nasopharyngeal carcinoma, oesophagus cancer etc. and having integrated clinical data and pathology features are selected. Through in situ hybridization, testing mRNA of relevant gene and expression of protein on tissue chip, consistent result between the invented product and traditional test is validated. In the product, cellular morphology is clear and even, and there is no fallen off tissue point. The invention is applicable to filter cancers, early diagnosis and forecasting prognosis.

The invention discloses rhodococcus ruber YMHL-1 capable of degrading nicosulfuron and applications thereof. The invention provides a strain namely rhodococcus ruber YMHL-1 capable of degrading residual nicosulfuron in wastewater, and the identification shows that the strain belongs to Rhodococcus. The strain is preserved in China General Microbiological Culture Collection Center, CGMCC in February 9th, 2015; and the preservation number is CGMCC No. 10542. The main biological characteristics are as follows: the Gram staining reaction is negative; the bacterial colony is in an orange yellow color, is in a round shape, and is opaque, the surface of the bacterial colony is rough and dry; the cell is in a sphere shape or short bar shape, does not have any flagellum, is motionless, and does not generate any spore; the strain is aerobic and chemoheterotrophic and cannot liquefy gelatin and hydrolyze starch; the enzyme contact reaction is positive, the V.P. reaction is positive, the methyl red reaction is negative; the strain cannot degrade casein, cannot reduce nitrates; and the strain can produce acids from glucose, can utilize glucose, fructose, and citrates, cannot utilize mannose, arabinose, and raffinose, and can reduce more than 90% of antibiotics in water through direct application.

The invention discloses zaralenone degrading bacteria and application thereof, and belongs to the technical field of biology. ZEN is used as a unique carbon source and energy to carry out preliminary screening and secondary screening, a bacteria strain H6 (CCTCC NO: M2016129) capable of degrading ZEN effectively is separated out, fermentation liquor of the bacteria H6 and ZEN (with final concentration of 10 (mu)g/mL) are cultured for 72 hours under the conditions of 37 DEG C and 180 r/min, and the degradation rate to ZEN reaches 93.64%. Through cellular morphology and physiological and biochemical identification and 16 S rRNA sequence comparative analysis, the strain of bacteria is identified as the bacillus amyloliquefaciens. The degradation active substances of the bacteria strain H6 mainly exist in fermented supernate, thalli also have partial degradation effect. The bacteria strain H6 can be used for preparing a microorganism preparation for degrading zaralenone or a feed biodegradation additive.

The invention relates to a quantitative analysis of an apoptotic cell morphology, which can effectively divide cell individuals from an image, automatically calculate cell two-dimensional morphology parameters and greatly shorten image analyzing time. The technical scheme adopted by the invention is a quantitative analysis method aiming at the apoptotic cell morphology of a fluorescence microscopic image. The quantitative analysis method comprises the following steps: (1) collecting a plurality of pairs of fluorescence microscopic cell images to be treated; (2) applying median filtering to a light microscope image in one pair of the images; carrying out gray stretching and carrying out image pre-treatment by using morphology opening operation; (3) automatically finding a threshold value by using an Otsu algorithm; (4) obtaining a divided binary image; (5) filling holes by using a morphology expansion and corrosion method, and removing small regions and incomplete targets on the boundary; (6) judging the state of a cell by the divided binary image according to a dyeing result; (7) outputting a parameter calculation result of each cell. The invention is mainly applied to the quantitative analysis method for the apoptotic cell morphology.

The invention discloses a cryopreserving solution for clinical-grade CAR-T cryopreservation and capable of being directly reinfused through intravenous infusion. The cryopreserving solution comprisesthe following raw materials: dimethyl sulfoxide, a glycerol fructose sodium chloride injection, an invert sugar electrolyte injection, a dextran 40 glucose injection, a hydroxyethyl starch 130/0.4 electrolyte injection, a vitamin C injection, a human serum albumin injection and a 0.9 % sodium chloride injection; the cryopreserving solution has the pH value of 6.8-7.0. When a formula for the clinical-grade CAR-T cryopreservation and capable of being directly reinfused through the intravenous infusion is used for cryopreserving CAR-T cells, the thawed cells and the thawed cryopreserving solutiondo not need centrifugation, resuspension, fluid exchange and other processes and can be directly reinfused through the intravenous infusion, so that the loss of cell varieties due to pollution in-vitro repeated proliferation of the CAR-T cells, or changes of cell morphologies and cell functions are effectively avoided, medical equipment can be also significantly simplified and the workload of medical workers can be reduced.

The method uses the anaerobic glove case and many modified culture medium to screen the anaerobic bacteria which possesses the highly effective character of degrading chlorophenol and acid resistance. The method comprises the following steps: A. screening the anaerobic particle sludge; B. segregation and purifying bacteria; C. primary screening the anaerobic bacteria degrading chlorophenol; D. second screening: segregation and purifying with the solid-liquid culture medium, until the cell shape being the single thallus. The operation is simple, and the theory meaning and economic value are important. The method provides the theory basis for anaerobic biological treatment process of chlorophenol waste water. The method can be used to separate, screen and culture the chlorophenol degradation bacterium, and provide the good screening method for researching chlorophenol degradation rule and interaction between function bacteria.

The invention includes sensors and sensing methods for determining cell morphology and/or chemical composition of an analyte. A porous substrate exhibiting a first optical signal is exposed to a target analyte and subsequently monitored for changes in the optical signal. More specifically, a photonic or porous substrate having a well-defined and highly tunable reflectivity or transmission spectrum, such as porous silicon (Si), porous alumina, porous Ge, porous GaAs, porous SiO2 and porous polymer, is used for example. A porous or photonic substrate is exposed to an analyte, such as a cell or other macromolecule, and changes in the scattered light are observed over time to determine cell morphology and/or chemical composition of the analyte using the substrate.

The invention relates to a serum-free medium used for culturing Vero cells. The serum-free medium is composed of, by volume, 85 to 95% of a base culture solution, 2 to 5% of an amino acid solution, 2 to 7% of a serum alternative factor solution, 0.1 to 0.5% of an antioxidant solution, 0.8 to 2% of a yeast extract solution, and 0.1 to 0.5% of an ethanol amine solution. The invention also provides a preparation method of the serum-free medium. In the preparation method, the base culture solution, the amino acid solution, the serum alternative factor solution, the antioxidant solution, the yeast extract solution, and the ethanol amine solution are mixed at the above ratio so as to obtain the serum-free medium. The serum-free medium used for culturing Vero cells is capable of promoting rapid attachment of Vero cells; cell morphology of the Vero cells cultured with the serum-free medium can be maintainer preferably, and cell proliferation rate is higher; the composition of the serum-free medium is simple; cost is reduced; and preparation is convenient.

The invention discloses a cell electrochemical sensor for analyzing joint toxicity of fungaltoxin, belonging to the fields of analysis and detection of food quality. According to the cell electrochemical sensor, the sensitivity of the sensor is increased by virtue of electroplating gold nanoparticles, an in-vivo physiological living environment of cells is simulated by virtue of laminin, and a three-dimensional model is constructed by virtue of rat tail collagens so as to relatively truly reappear cell morphology; and the constructed cell electrochemical sensor can rapidly and sensitively respond to the influences caused by the fungaltoxin to human hepatoma carcinoma cells, equipment is convenient and rapid and low in production cost, and by combining with an electrochemical resistance value and a joint index method, the type and degree of a joint action of the fungaltoxin can be rapidly judged. The constructed portable cell electrochemical sensor is successfully applied to the toxicity analysis of the fungaltoxin in foods and has good application prospects in the analysis of the joint toxicity of the fungaltoxin.

The invention discloses a high-efficiency non-integrated human iPSC induction platform which comprises a composition, wherein the composition is used for inducing a human cell into iPSC, and the composition comprises a previous inducer and a later inducer; the previous inducer comprises the following active components: a transforming growth factor beta inhibitor, a glycogen synthetase kinase 3 inhibitor, a cAMP agonist, an S-adenosyl homocysteine hydrolase inhibitor and a p21 activated kinase inhibitor; and the later inducer comprises the following active components: the glycogen synthetase kinase 3 inhibitor, a selective ATP noncompetitive MEK inhibitor and the S-adenosyl homocysteine hydrolase inhibitor. The high-efficiency non-integrated human iPSC induction platform disclosed by the invention achieves the previous induction efficiency of the iPSC of 6.4% under the action of a previous inducer composition, can achieve the induction of a full culture of 20.8% through later induction culture or be used for totally further inducing a previous inductor clone into a human iPSC clone approaching to ESC and is far higher than the prior art in induction efficiency; in addition, the obtained iPSC is high in maturity, free of being inserted with an exogenous gene, is more approaching to the ESC in cell morphology and property and has the advantages of good stability and great high application value.