Screening method for anaerobe capable of degrading chlorophenol

Abstract

The method uses the anaerobic glove case and many modified culture medium to screen the anaerobic bacteria which possesses the highly effective character of degrading chlorophenol and acid resistance. The method comprises the following steps: A. screening the anaerobic particle sludge; B. segregation and purifying bacteria; C. primary screening the anaerobic bacteria degrading chlorophenol; D. second screening: segregation and purifying with the solid-liquid culture medium, until the cell shape being the single thallus. The operation is simple, and the theory meaning and economic value are important. The method provides the theory basis for anaerobic biological treatment process of chlorophenol waste water. The method can be used to separate, screen and culture the chlorophenol degradation bacterium, and provide the good screening method for researching chlorophenol degradation rule and interaction between function bacteria.

Description

technical field

[0001] The invention relates to a method for screening anaerobic bacteria capable of degrading chlorophenols. belongs to the field of microbiology. Background technique

[0002] Chlorophenols are a class of chlorinated hydrocarbons, insecticides or antimicrobial agents used for non-agricultural insecticides. The concentration threshold of its toxic effect on the human body is only 40ppm, the threshold of its toxic effect on fish is 1ppm, and 1ppb of chlorophenol in water can cause water pollution. Listed by the US EPA as Priority Pollutants and China's Blacklist of Key Pollutants. At present, the ultrafiltration method, chemical precipitation method, biological method and other treatment methods commonly used for chlorophenols have high energy consumption, high cost, and secondary pollution caused by chemical addition. Therefore, the anaerobic biological treatment technology of p-chlorophenol, a refractory organic matter, will pro...

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