Substrate for tissue culture of dracocephalum rupestre hance as well as preparation method and application thereof
A tissue culture and liquid culture medium technology is applied in the field of substrates for tissue culture and preparation thereof, and can solve the problems of limited geographical location, low seedling efficiency, large sample consumption, etc. Stress resistance, improve seedling rate, fast growth effect
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Embodiment 1
[0028] A kind of substrate for tissue culture of hairy grass, made of the following concentrations of substances: BA 0.5mg / L, IBA0.2mg / L, sucrose 20g / L, goosegrass powder 5g / L, agar 5g / L, solvent is 1 / 2MS liquid medium. Goosegrass powder is the whole grass powder of goosegrass dried to a moisture content of 4.3%, with a particle size of 200 mesh.
Embodiment 2
[0030] A kind of substrate for tissue culture of hairy grass, made of the following concentrations of substances: BA 0.2mg / L, IBA0.1mg / L, sucrose 15g / L, goosegrass powder 10g / L, agar 10g / L, solvent is 1 / 2MS liquid medium. Goosegrass powder is the whole grass powder of goosegrass dried to a moisture content of 4.3%, with a particle size of 100 mesh.
Embodiment 3
[0032] A kind of substrate for tissue culture of hairy grass, made of the following concentrations of substances: BA 0.3mg / L, IBA0.15mg / L, sucrose 10g / L, goosegrass powder 8g / L, agar 8g / L, solvent is 1 / 2MS liquid medium. Goosegrass powder is goosegrass root powder dried to a moisture content of 3.8%, with a particle size of 200 mesh.
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