The present application relates to the technical field of separation and purification of
Schizonepeta tenuifolia Briq, and relates to a
biphenyl compound in
Schizonepeta tenuifolia Briq, a preparation method thereof and application of the
biphenyl compound as an anti-inflammatory
drug. The
biphenyl compound in
Schizonepeta tenuifolia Briq is obtained by the following steps: soaking
Schizonepeta tenuifolia Briq, heating and
reflux extraction, recovering and concentrating under reduced pressure, obtaining total extract of
Schizonepeta tenuifolia Briq, extracting and concentrating with
petroleum ether,
dichloromethane and
ethyl acetate, obtaining a
dichloromethane part extract,
gradient elution separation of the
dichloromethane part extract,
gradient elution separation of the fourth fraction of the obtained eight fractions,
gradient elution separation of the third fraction of the obtained six fractions, gradient
elution separation of the fifth fraction of the obtained eight fractions, purification and separation of the eluate, and collection of the eluate, and the
biphenyl compound in
Schizonepeta tenuifolia Briq is obtained at 16.4 minutes. The present application discloses the
biphenyl compound in Schizonepeta tenuifolia Briq for the first time, and
in vitro anti-inflammatory pharmacodynamic experiments are carried out, which prove that the
biphenyl compound has a certain
inhibitory effect on RAW264.7 cells and can be applied to preparation of anti-inflammatory drugs.