117 results about "Rat tail" patented technology

The invention relates to a method for constructing a three-dimensional neural stem cell model in two steps by adopting the micro-fluidic technology. The method is characterized in that a rat tail collagen I is used as a three-dimensional support, a micro-pillar array type micro-fluidic chip is used as a culture platform, and a neural stem cell is cultured in two steps, wherein in the early culture stage, a culture medium for promoting the amplification of the neural stem cell is injected into a cell culture chamber, and in the later culture stage, a conditioned medium suitable for the growth of the neural stem cell and the daughter cells thereof is used, and a three-dimensional composite structure which is similar to a nerve tissue is formed by simulating the microenvironment of different neurogenesis stages in the body. The method provided by the invention is good in repeatability and can be used for construction a plurality of groups of samples. The adopted microfluidic culture system is in a microliter volume and can be regulated accurately, thus the amount of various high-cost cell growth factors, immunologic fluorescent antibodies and cell hormones used in the process of culturing the cell can be reduced greatly, and the cell culture cost can be lowered. The three-dimensional neural stem cell model is expected to be a nerve tissue substitute for screening a novel medicament or monitoring an environmental toxin.

The present invention relates to a method for inducing the bionic calcification in collagen fibers through a polymer polyelectrolyte, and applications thereof. The method comprises: treating different types of collagen scaffolds in a calcium phosphate mineralized solution containing a cationic polyelectrolyte or anionic polyelectrolyte to obtain the bionic calcification collagen scaffold material having the inside-fiber mineralization characteristic, wherein the collagen scaffold is recombinant collagens type I having different sources, completely demineralized bone collagen, completely demineralized dentin collagen, or rat tail collagen, and the mineralization liquid is a calcium phosphate solution added with a cationic polyelectrolyte or anionic polyelectrolyte. According to the present invention, through the stabilizing effect of the polyelectrolyte on the calcium phosphate solution, the novel bionic calcified collagen scaffold material with characteristics of similar natural bone tissue structure, good mechanical property, good biocompatibility and suitable biodegradability is constructed.

The invention discloses a method for culturing bladder cancer organs in vitro. The method comprises the following steps: manufacturing a gas-liquid interaction culture system, namely manufacturing a uniformly paved rat tail collagen supporting layer on the surface of a porous culture membrane in a Transwell upper chamber; re-suspending fresh in-vitro bladder cancer tissues by using a rat tail collagen solution, uniformly mixing, adding the obtained mixture onto the rat tail collagen supporting layer, and then putting the rat tail collagen supporting layer into an incubator at 37 DEG C to solidify so as to obtain a rat tail collagen layer containing the bladder cancer tissues; adding an organoid culture medium into a Transwell lower chamber, and enabling the liquid level of the culture medium to be lower than the rat tail collagen layer containing bladder cancer tissues; and carrying out passage and cryopreservation. The culture success rate of the bladder cancer organoid is remarkablyincreased, the bladder cancer organoid with reserved immune cells can be obtained through culture, operation is easy, the utilization rate of tumor tissue is high, and great significance and value areachieved for bladder cancer drug screening research.

The invention discloses a cell electrochemical sensor for analyzing joint toxicity of fungaltoxin, belonging to the fields of analysis and detection of food quality. According to the cell electrochemical sensor, the sensitivity of the sensor is increased by virtue of electroplating gold nanoparticles, an in-vivo physiological living environment of cells is simulated by virtue of laminin, and a three-dimensional model is constructed by virtue of rat tail collagens so as to relatively truly reappear cell morphology; and the constructed cell electrochemical sensor can rapidly and sensitively respond to the influences caused by the fungaltoxin to human hepatoma carcinoma cells, equipment is convenient and rapid and low in production cost, and by combining with an electrochemical resistance value and a joint index method, the type and degree of a joint action of the fungaltoxin can be rapidly judged. The constructed portable cell electrochemical sensor is successfully applied to the toxicity analysis of the fungaltoxin in foods and has good application prospects in the analysis of the joint toxicity of the fungaltoxin.

The invention discloses a seedling producing technique for rat-tailed algae, applying to seedling breed in-door according to sexual reproduction law, including: selecting and collecting the seed algae, stimulating and diffusing the seed algae, collecting the juvenile sporophyte, culturing and catadromousing seedlings, provided an artificial seedling producing technique for rat-tailed algae, dissolving the current shortcoming problem of the rat-tailed algae seedlings.

The invention provides a method for separating and cultivating rat hepatocytes. A rat is anaesthetized by Nembutal and is given sodium heparin for anticoagulation; portal catheterization is carried out; two-step reperfusion is carried out for perfusion; a digestive mature liver is taken off; hepatocytes are collected in Hanks liquid containing 1 percent of bovine serum albumin (BSA); hepatocyte suspension is screened by a screen stencil; after the filtrate is centrifugated and deposited, the deposit is collected in Leibovitz's-15(l-15) complete medium; the density of hepatocyte is adjusted to 3 to 6 *10<5>/ml and inoculated on a culture plate coated with rat tail collagen in the environment of temperature of 37 DEG C and 5 percent of CO2; and unattached and dead hepatocytes are removed by changing medium after 4 hours of inoculation, and the hepatocytes can be used for experiment after 20 hours of cultivation. The method is easy, simple and stable, has low cost and high efficiency, can obtain high-activity hepatocytes, is easy to attach, and can be used for pharmacological and toxicity experiments and popularized in common laboratories.

The invention discloses an endothelial progenitor cell (EPC) culture method. The culture method comprises the following steps: inoculating human peripheral blood mononuclear cells in a cell culture bottle coated by I-type rat tail collagen, adding a DMEM culture medium in the cell culture bottle, putting the cell culture bottle in an incubator with the temperature of 37 DEG C, the CO2 content of 5 percent and the humidity of 95 percent for culturing; carrying out EPC digestion passage; then carrying out flow cytometric analysis on EPC; carrying out EPC immunofluorescence assay; carrying out a cell proliferative assay; carrying out a single cell clonogenic assay; carrying out FITC-UEA-1 extracellular adsorption and endocytosis Dil-acLDL experimental measurement; and carrying out an in-vitro tube formation experiment. The EPC culture method is simple, convenient and feasible. With the adoption of the EPC culture method, the cost can also be greatly lowered; the high-purity I-type rat tail collagen is prepared by the EPC culture method and used for culturing EPC by coating the cell culture bottle, and the culture medium which is low in cost is also adopted, so that the EPC is successfully cultured from human peripheral blood; through identification, the EPC cultured by the method disclosed by the invention has the phenotypic characteristics and the functions of the EPC cultured by the conventional method.

The invention provides an in vitro tumor cell 3D collective invasion model and a construction method thereof. Tumor multicellular organ groups are prepared; cells contain light conversion fluorescentprotein which is coated into type I rat-tail collagen for carrying out 3D culture and the growth state, a proliferation mode and an invasion mode of tumor cells are simulated in vitro. The model studies the collective invasion effect of tumor multicellular spherical organs and can simulate the collective invasion mode of the tumor cells in vivo; after photobleaching is carried out by using a laserconfocal microscope, Leader Cells and Follower Cells in the collective invasion cells can be distinguished under the condition of lacking specific biological markers; in addition, the tumor cells ina target region can be obtained by fluorescence flow separation for carrying out follow-up experimental study.

The invention discloses a method for storing spermatophore of litopenaeus vannamei at low temperature. The method comprises the following steps of: acquiring the spermatophore, fixing the spermatophore, storing the spermatophore and dyeing spermatozoa. In the method, a culture layer for the spermatophore of the litopenaeus vannamei is prepared from 0.3 to 0.4 part of 1.8 to 3.0 percent self-prepared type I liquid rat tail collagen serving as a medium for contracting the culture layer, 0.3 to 0.4 part of culture solution of 2*1,640, 0.1 to 0.2 part of fetal calf serum in an American GIBCO company and 0.1 part of Matrigel (a soluble basilar membrane matrix) in an American Sigma company, wherein the culture layer is viscous at normal temperature, and can be solidified at the temperature of 40 DEG C and can wrap the spermatophore. The spermatophore is packaged in separate an epoxy resin (EP) tube at the normal temperature, the spermatophore is wrapped by the prepared culture layer, and the EP tube wrapped with the spermatophore is arranged in a refrigerator to be stored at the low temperature of 4 DEG C. The method has the characteristics of simplicity of operation, complete storage of a spermatophore structure, high survival rate of the spermatozoa, long retention time, stable effect of low-temperature storage and the like and is easy to popularize and apply. The retention time of the spermatophore is at least 3 months, and the survival rate of the spermatozoa is over 80 percent.

The invention discloses a non-invasive rat tail blood pressure measuring device which comprises an experiment tray and also comprises a rat body fixing component and a pulse detecting component. The rat body fixing component and the pulse detecting component are arranged on the experiment tray, the rat body fixing component is used for limiting the body of a rat and keeping the rat calm, and the pulse detecting component is used for measuring rat tail blood pressure. The device can effectively limit the bodies and tails of different sizes of rats, especially small sizes of rats, keep the rats calm and facilitate rat tail blood pressure measurement, and the measurement accuracy is effectively improved.

The present invention discloses a culture method for eriones unguiculatus primary liver cells. The method comprises: (1) collecting a liver cell primary suspension from digested and matured isolated eriones unguiculatus liver, filtering, removing impurities from a filtrate, adding 5% calf serum-containing HBSS, repeatedly carrying out centrifugation washing, collecting cell precipitate and adding to a 20% new born calf serum-containing DMEM complete culture medium, and adjusting a liver cell concentration to 3.0*10<5>-5.0*10<5>/mL to prepare a liver cell suspension; and (2) inoculating the liver cell suspension in a culture plate coated with rat tail collagen, culturing at a temperature of 37 DEG C in a 5% CO2 environment, removing impurities after 4 hours, changing the culture medium into a maintenance culture medium containing cell factors, and carrying out cell adherence overnight to obtain the eriones unguiculatus primary liver cells. The method has characteristics of low cost, economy, practicality, and simple and easy-performing operation, wherein the obtained liver cells have characteristics of strong adherence, high vitality, high survival rate, stability and reliability, and the method is suitable for mass production.

The invention provides a device and method for patterning co-culture of multiple cells. Adhesive tape is punched through a punching needle to form an array with micrometer-order holes, the array is used as a template, polydimethylsiloxane is directly poured to the template to obtain a channel with micrometer-order pillar array inside, polydimethylsiloxane is printed on a cell petri dish or culture plate base, the surface of the cell petri dish or culture plate base is not processed, or a rat tail collagen layer is incubated on the surface to form the culture device. The method of cell culture through the device is provided, the device can be manufactured just by the adhesive tape, the punching needle, a nicking tool and polydimethylsiloxane without photolithography, the device can be used for patterning any kind of cells, co-culture of the multiple cells is achieved, and limitation to photolithography by traditional micro-fluidic chip preparation is broken through.

The invention belongs to the field of cosmetics research and development, and particularly relates to application of rat tail collagen in the field of cosmetics. Compared with conventional collagen onthe market, in addition to the characteristics of general collagen, the rat tail collagen has the advantages of stable triple helix structure, high collagen activity and low immunogenicity; the source quality is controllable, the biosafety is high, and the reduction of biological activity by radiation sterilization is not needed; through experimental determination, the rat tail collagen preparedaccording to the technical scheme has the performance of whitening, melanin synthesis inhibition, anti-oxidation, anti-allergy, hormone skin repair and acne removal. The application of the rat tail collagen in the field of cosmetics overcomes the technical defect that the quality of the cosmetics is seriously affected due to addition of toners, flavoring agents, stabilizers and preservatives intocollagen-containing cosmetics in the prior art.

The invention relates to a method for separating and culturing macaque adult hepatic precursor cells, in particular to a separation and culture solution of the macaque adult hepatic precursor cells, belonging to a separation and culture solution of animal precursor cells. The method comprises the following steps: digesting a small quantity of hepatic tissues obtained by a macaque liver removal surgery or other ways by utilizing IV-type collagenase; collecting and suspending the cells in 10% of a fetal calf serum DMEM for culture; selecting and then inoculating a single cell block in the culture plate of a rat tail collagen bed board, wherein the culture solution is the special one; and finally sorting E-cad+cells from the cultured cells by the flow cytometry to obtain the target cells. The cells separated by the method of the invention can be applied to cell differentiation mechanism study and can become ideal master cells for treating late-period hepatic diseases.

The invention discloses a membranous matrix (cytoskeleton) which is prepared by mixing collagen and chitosan and then drying the mixture. The preparation process is simple. The collagen component is collagen I extracted from the rat tails, accounting for 90% of the weight of the mixture. The chitosan component is 75-95% of deacetylated chitosan, accounting for 10% of the weight of the mixture. The invention adopts the composite membrane as the cytoskeleton for constructing the tissue-engineered tympanic membrane and finds that the composite membrane is suitable for grow and proliferation of epithelial cell/ keratinocyte and fibroblast of the tympanic membrane and can maintain good collagen secretion function of the fibroblast. The cytoskeleton further lays the foundation for constructing the tissue-engineered tympanic membrane.

The invention relates to a changeable square fixing device. The fixing device is provided with a base, a movable baffle, a fixed cylindrical body and a movable cylindrical body. The fixed cylindrical body and the movable cylindrical body are located on the base, and an arc-shaped cavity is formed between the fixed cylindrical body and the movable cylindrical body. The movable cylindrical body is provided with a radial sliding rail, and the sliding rail is matched with a radial sliding block on the base and can accordingly move on the base in the radial direction. One end of the fixed cylindrical body is provided with a tapered limiting plate. The movable baffle is provided with a fixing inserting plate and a stepped supporting plate. A U-shaped groove and a movable guide rail are arranged at the upper end and the lower end of the stepped supporting plate respectively. The movable baffle can axially move on the base through match of the movable guide rail and an axial sliding block of the base. The fixing inserting plate is provided with a rat tail groove and is embedded in the arc-shaped cavity between the fixed cylindrical body and the movable cylindrical body. The changeable square fixing device has the advantages that different sizes of rats can be fixed by adjusting the fixed space, the fixed rat body can be exposed outside, experiment research is facilitated, and the fixing device is simple in structure and reliable in fixing.

The invention relates to a rat caudal vertebra intercalated disc minimally invasive puncture injection device which is used for conducting minimally invasive puncture or injecting liquid on a fibrous ring in a rat tail. The rat caudal vertebra intercalated disc minimally invasive puncture injection device comprises a lantern ring, a needle handle and a needle plug. The lantern ring is of a hollow cylinder structure, the middle of the lantern ring is a sleeving hole used for sleeving the rat tail, a needle handle insertion hole and a locating hole are formed in a lantern ring body, and the needle handle insertion hole and the locating hole are arranged in a spaced mode and both communicated with the sleeving hole; the needle handle comprises a clamping portion of the upper end, a middle insertion portion and a needle, the insertion portion can be inserted and connected in the needle handle insertion hole, and a needle plug hole is formed in the clamping portion; the needle plug can be inserted into the needle plug hole to block the needle handle. Compared with the prior art, according to the rat caudal vertebra intercalated disc minimally invasive puncture injection device, the lantern ring is fixed on the rat tail, and the needle handle is inserted into the lantern ring, so that the minimally invasive puncture to the rat tail is achieved; the structure is simple, and the operation is convenient; meanwhile, the stability to the rat tail is stronger, the lantern ring can rotate on the rat tail, and therefore a circle of minimally invasive puncture on the fibrous ring can be conducted.

The invention discloses an experimental rat tail-cutting blood collection device in the field of experimental apparatuses. The experimental apparatus comprises a front chamber and a rear chamber in sequence. The front chamber and the rear chamber are fixedly connected to each other. The front chamber comprises a trapezoidal plate and a conical plate. Two bevel edges of the trapezoidal plate are fixedly connected to a bottom edge of the conical plate, respectively. The rear chamber comprises a rectangular plate and an arch plate. Two long edges of the rectangular plate are fixedly connected to a bottom edge of the arch plate respectively. A plurality of air vents are circumferentially formed in the conical plate and the arch plate in uniform distribution. One end of the arch plate is hinged to a door sheet matching with the arch plate, and a concentric through hole is formed in the door sheet. A locking groove is formed in the edge of the door sheet, and a connecting buckle fastened to the locking groove is disposed on the rectangular plate. By using the experimental rat tail-cutting blood collection device, as an experimental rat lies in the device, the four limbs of the experimental rat cannot straighten; thus, the experimental rat can be prevented from moving in the front chamber and the rear chamber, fixation of the experimental rat is achieved, and the problem of unsuccessful blood collection caused by the fact that the cut tail enters a plastic bottle due to restless movement of the experimental rat is avoided.

The invention discloses an in vitro culture method of cervical intraepithelial neoplasia cells. The in vitro culture method comprises the following steps: step one, taking a small piece of human cervical neoplasia tissue; step two, after digesting and decomposing by a type I collagenase, preparing a cell suspension; step three, utilizing a trypan blue dye exclusion method to judge the cell activity; step four, introducing the cell suspension into a culture bottle containing a low fetal bovine serum culture medium and coated with rat tail collagen, and waiting for the cells to adhere to the wall; and step five, after the cells adhere to the wall, replacing with a serum-free culture medium, purifying the cervical intraepithelial neoplasia cells, replacing the culture liquid weekly for at least two times, and after the cells are fused to 80%-85%, carrying out passage. The CIN cells cultured by the method have high survival rate and high purity, allows the morphology of the cells after passage to have no obvious change compared with that of the primary cells, and is carried with human papillomavirus (HPV); and the success of the CIN cell in vitro culture provides technical support for research on treatment of cervical cancer.

The invention belongs to the technical field of tissue engineering, and provides a preparation method of a tissue-engineered scar skin model. Scar tissue acellular dermal matrix protein, scar fibroblasts and rat tail collagen are subjected to tissue engineering skin culture so as to obtain tissue-engineered skin. The method comprises the following steps: S1, extracting acellular dermal matrix protein of scar tissue; S2, extracting scar fibroblasts; S3, carrying out mixed culture on the scar tissue acellular dermal matrix protein obtained in the S1, the scar fibroblasts obtained in the S2 and the rat tail collagen; and S4, after one week of culture, adding epidermal stem cells, and performing culturing until the tissue-engineered skin is formed. The technical scheme solves the problem of lack of a proper human scar model in the prior art.

The invention relates to a rifle accessory and in particular relates to a 95 rifle tactic extension system with a laser sight. The 95 rifle tactic extension system with the laser sight. The 95 rifle tactic extension system comprises a main body, an auxiliary body, a connecting bolt, a gun barrel clamp and the laser sight; the main body comprises an upper cover and a sidewall; the laser sight comprises a laser module and a rat tail control switch; the laser module is arranged inside the main body; the circuit and the battery are arranged in the rat tail control switch; the rat tail control switch is arranged on the upper cover, the sidewall or the auxiliary body; the laser module, the battery and the rat tail control switch are connected by use of the circuit; the main body and the auxiliary body are connected together by use of the connecting bolt; the gun barrel clamp is arranged on the front lower end of the sidewall; the gun barrel clamp is mounted on the gun barrel of the 95 rifle tactic extension system. The 95 rifle tactic extension system with the laser sight can be used as a guide rail and also is provided with the laser sight.

The invention discloses a rat tail cutter which comprises a box body, a rat tail clip and a cutting blade. Both ends of the box body are provided with openings, and the outer walls of the tops of thetwo ends of the box body are provided with an inlet plate insertion groove and an outlet plate insertion groove respectively. Equidistantly-distributed baffle insertion grooves are formed in the outerwall of the top of the box body, and the baffle insertion grooves are located between the inlet plate insertion groove and the outlet plate insertion groove. The vertical sections of the inlet plateinsertion groove, the outlet plate insertion groove and the baffle insertion grooves each are of a C-shaped structure. An inlet plate is inserted in the inlet plate insertion groove and includes a porous inlet plate and a clamping piece plate. A rat head clamping plate is inserted in the outlet plate insertion groove, and baffles are inserted in the baffle insertion grooves. According to the rat tail cutter, the position of a support can be adjusted according to the length or weight of the rat and the part of the rat to be exposed, an adjustment can be made according to sizes and lengths of different rats, the rat tails and rat heads can be fixed, to facilitate the taking of the rat tail and the marking on the rat ear, the time is saved, and a sample can be protected from pollution.