Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of rat tail collagen protein

A technology of rat tail collagen and collagen, which is applied in the field of preparation of rat tail collagen, can solve the problems of complex separation and purification process, low extraction yield, and no large-scale production, so as to simplify the extraction process, increase the extraction rate, simplify The effect of process and operation process

Inactive Publication Date: 2012-07-18
广东省医学实验动物中心
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the shortcomings of the existing method for preparing rat tail collagen, such as low extraction yield, complicated separation and purification process, and no method for large-scale production, and provide a product with simple process, high extraction rate, and high extraction rate. Preparation method of high-purity rat tail collagen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of rat tail collagen protein
  • Preparation method of rat tail collagen protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Preparation of Rat Tail Collagen

[0027] The rat tail collagen involved in the present invention is extracted from the rat tail of SPF grade (specific pathogen free) adult SD rats. Rats were sacrificed by short neck. The SD rat tail was removed mechanically. Remove the entire exposed rat tail with medical scissors, discarding the outer skin. The rat coccyx was broken but not broken into small pieces. Use medical hemostatic forceps to extract the rat tail tendon from the thicker base of the rat tail. Rat tail collagen extraction of the present invention comprises the following steps:

[0028] 1. Preparation and configuration of buffer solution: 0.1-5mol / L NaCl solution, 0.01-0.2mol / L Tris-HCl buffered saline solution, 0.05-2mol / L acetic acid solution.

[0029] 2. Separate the rat tail tendon from the rat tail. The extracted rat tail tendon was placed in 0.01-0.2 mol / LTris-HCl buffered saline solution at 4°C, and the extracted tail tendon was silver-whit...

Embodiment 2

[0038] Embodiment 2: the preparation process of embodiment 1 rat tail collagen

[0039] The temperature of the Tris-HCl buffered saline solution in step 2 in Example 1 is adjusted to 0°C; the pretreatment time of step 3 is adjusted to 4 hours, and the rat tail tendon is placed in 0.05mol / L acetic acid solution at 0°C; step 4 stomach The ratio of protease is 1:1000, and the enzymatic hydrolysis lasts for 36 hours; step 5, centrifuge the latex solution at 1000rpm / min for 40 minutes; steps 6 and 7, centrifuge the latex solution at a high speed of 4000rpm / min for 50min; step 8, dialysis in the dialysis bag 48h, adopt shaker, low speed (1-5 rev / min), adopt silver nitrate solution to check that the dialysis external fluid has no precipitation, show that dialysis is completed, all the other are the same as embodiment 1.

Embodiment 3

[0040] Embodiment 3: the preparation technology of embodiment 1 mouse tail collagen

[0041] The temperature of the Tris-HCl buffered saline solution in step 2 in Example 1 is adjusted to 10°C; the pretreatment time of step 3 is adjusted to 36 hours, and the rat tail tendon is placed in 2mol / L acetic acid solution at 10°C; step 4 pepsin The ratio is 1:50000, and the enzymatic hydrolysis lasts for 144 hours; step 5, centrifuge the latex solution at 8000rpm / min for 10 minutes; steps 6 and 7, centrifuge the latex solution at a high speed of 12000rpm / min for 20min; step 8, dialyze in the dialysis bag for 96h , adopt shaking table, low speed (1-5 rev / min), adopt silver nitrate solution to check that the dialysis external liquid has no precipitation, show that dialysis is completed, and all the other are with embodiment 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of a rat tail collagen protein. A novel extraction scheme for mixing an enzymic method and an acid method is adopted, and a method for salting out and centrifuging by using solutions with different salinities for many times is adopted, so that the purification step is greatly simplified. By using the preparation method used by the invention, collagen proteins with the dry weight of 200mg can be extracted from each rat tail, the dry weight of the collagen proteins is increased by about 20 times than 10mg of collagen proteins extracted from each rat by using the traditional acid-leaching method, and defects and problems such as low extraction ratio, difficulty in purification, damage to the biological activity of the collagen protein and the like in the traditional method are avoided. Appraised by SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophresis), the obtained rat tail collagen protein obtains stripes with three clauses, and compared with the traditional commercial rat tail collagen protein of the SIMGA company, the rat tail collagen protein provided by the invention has no difference for atlases. Through analyzing the content of the amino acid, the prepared rat tail collagen protein is high in quality and purity.

Description

【Technical field】 [0001] The invention relates to a preparation method of rat tail collagen. In particular, it relates to a preparation method suitable for industrial production of rat tail collagen. 【Background technique】 [0002] Collagen is an important functional protein. Collagen contains a unique amino acid - hydroxyproline (Hyp). Collagen is closely related to cell proliferation, differentiation, exercise, immunity, and joint lubrication. Widely used in medicine, food, daily chemical industry, biosynthesis and other fields, such as medical capsules, edible gelatin, photographic gelatin, cosmetics, etc. Rat tail collagen is a type of collagen, mainly type I collagen. Type I collagen is mainly found in the dermis, tendon, bone, and dentin. Collagen is most commonly formed in the form of fibers, consisting of three strands intertwined to form groups of polypeptide chains. Rat tail collagen can be used to coat cell culture vessels and cultivate some cells that are not...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/30
Inventor 任海涛钟志勇唐小江邝少松刘科饶子亮严家荣郑佳琳王刚
Owner 广东省医学实验动物中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com