Method for preparing bacteria cellulose with association of activity and inertia

A technology combining bacterial cellulose, dynamic and static, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of reduced transmission rate, long production cycle, viscous fermentation broth, etc., and achieve simple process, Yield improvement effect

Inactive Publication Date: 2008-12-10
许春元 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the traditional static cultivation of bacterial cellulose membrane technology is relatively mature, and there are some successful application examples, its large-scale and high-quality Therefore, most scientific research teams are currently actively exploring the use of industrial fermentation tanks to ferment bacterial cellulose under dynamic conditions. However, there are still some problems in dynamic fermentation conditions, such as: cellulose-producing strains are easy to mutate into non-cellulose-producing strains This is mainly because as the culture time prolongs, the bacteria breed in large numbers, and the demand for oxygen also increases. At the same time, the continuous production of bacterial cellulose makes the fermentation broth very viscous, which leads to the transfer of oxygen from the gas phase to the liquid phase. In order to maintain enough dissolved oxygen for cell growth and bacterial cellulose synthesis, it is necessary to increase the stirring speed, and increasing the speed will lead to the emergence of mutant strains, the decline of cellulose production and the degradation of cellulose. Changes in shape and water absorption, degree of polymerization, etc. (eg US 4863565)
Therefore, the dynamic fermentation production method has not yet had an example of successful industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1. A method for preparing bacterial cellulose by combining static and dynamic, the steps are as follows: take the Acetobacter xylinum bacterial classification and insert it into the seed medium, shake it at 25° C., shake it at 100 rpm for 12 hours, and then transfer it to the seed tank with 4% inoculum The medium was expanded and cultured for 12 hours, the ventilation rate was 0.3vvm, the rotation speed was 100rpm, and the temperature was 25°C. Then transfer to the production tank for cultivation for 12 hours, the ventilation rate is 0.3vvm, the rotation speed is 60rpm, and the temperature is 25°C. Then subpackage and culture in a shallow plate for 8 days at a culture temperature of 26° C. to obtain a finished crude cellulose film.

[0019] The seed culture solution used is: 2.5% of glucose, 0.4% of corn steep liquor, 0.4% of ammonium sulfate, 0.2% of potassium dihydrogen phosphate, and 0.04% of magnesium sulfate.

[0020] The fermentation medium used is: gluc...

Embodiment 2

[0021] Example 2. A method for preparing bacterial cellulose by combining static and dynamic, the steps are as follows: take the Acetobacter xylinum bacterial classification and insert it into the seed culture medium, shake the table at 25°C, and shake it at 180rpm for 48h, then transfer it to the seed tank with 16% inoculum Medium expansion culture was carried out for 36 hours, the ventilation rate was 0.5vvm, the rotation speed was 60rpm, and the temperature was 30°C. Then transfer to the production tank and cultivate for 36 hours, the ventilation rate is 0.5vvm, the rotation speed is 120rpm, and the temperature is 30°C. Then subpackage and culture in a shallow dish for 4 days at a culture temperature of 31° C. to obtain a finished crude cellulose film.

[0022] The seed culture solution used is: 2% of glucose, 0.6% of corn steep liquor, 0.2% of ammonium sulfate, 0.4% of potassium dihydrogen phosphate, and 0.04% of magnesium sulfate.

[0023] The fermentation medium used i...

Embodiment 3

[0024] Example 3. A method for preparing bacterial cellulose by combining static and dynamic, the steps are as follows: take Acetobacter xylinum strains and insert them into the seed culture medium, shake the table at 32° C., and shake at 120 rpm for 24 hours, then transfer to the seed tank according to the inoculum size of 6%. Medium expansion culture was carried out for 24 hours, the ventilation rate was 0.4vvm, the rotation speed was 80rpm, and the temperature was 29°C. Then transfer to the production tank for 24 hours, the ventilation rate is 0.6vvm, the rotation speed is 80rpm, and the temperature is 29°C. Then subpackage and culture in a shallow dish for 5 days at a culture temperature of 29° C. to obtain a finished crude cellulose film.

[0025] The seed culture solution used is: 2% of glucose, 0.5% of corn steep liquor, 0.2% of ammonium sulfate, 0.4% of potassium dihydrogen phosphate, and 0.1% of magnesium sulfate.

[0026] The fermentation medium used is: glucose 2....

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PUM

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Abstract

The invention discloses a method for preparing bacterial cellulose by dynamic and static combination, which is characterized in that: an acetobacter xylinum strain is taken and inoculated into a seed culture medium, is inoculated into a seeding tank for amplification culture for 12 to 36h according to 4 to 16 percent of inoculum concentration after the shaking culture by a shaking table at a temperature of between 25 and 31 DEG C and a rotating speed of between 100 and 180rpm for 12 to 48h, is inoculated into a productive tank to be cultured for 12 to 36h, and is packaged separately and then undergoes tray culture for 3 to 10 days at a culture temperature of between 25 and 31 DEG C to produce a finished product, a crude cellulose membrane. The method has simple process, the output is apparently improved, and the output of the bacterial cellulose produced by the method is high up to 13.6g/L (dry weight).

Description

technical field [0001] The invention relates to a method for preparing bacterial cellulose, in particular to a method for preparing bacterial cellulose by combining dynamic and static methods. Background technique [0002] Bacterial cellulose is a research hotspot in the field of biomaterials at home and abroad. Its chemical composition is the same as that of plant cellulose, but bacterial cellulose has unique characteristics such as high crystallinity, high purity, high mechanical strength, ultrafineness and excellent biocompatibility. It has broad application prospects in the research and development of new energetic materials, food, medicine, papermaking, oil exploration, fuel cells and sewage treatment. [0003] Although the traditional static cultivation of bacterial cellulose membrane technology is relatively mature, and there are some successful application examples, its large-scale and high-quality Therefore, most scientific research teams are currently actively exp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/02
Inventor 许春元孙东平
Owner 许春元
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