271 results about "Acetobacter xylinum" patented technology

The invention provides compound probiotics fermented Chinese herbal medicine active health care liquid and a preparation method thereof. The health care liquid is prepared by the following raw materials: folium ginkgo, gingko pollen, fructus lycii, tea leaves, bacillus natto, saccharomyces cerevisiae, lactobacilli, acetobacter xylinum, bifidobactirium, white granulated sugar, brown sugar, defatted soy flour, honey, oligosaccharide, sodium chloride, deionized water and the like. The compound probiotics fermented Chinese herbal medicine active health care liquid integrates disease treatment and health care functions of several single products, contains Chinese herbal medicine active ingredients with the functions of preventing and treating hyperlipidemia, hyperglycemia and hypertension, preventing tumors and cancers, enhancing memory, protecting livers, improving immunity, preventing and treating coronary heart diseases, and also contains probiotics groups and metabolic products of the probiotics groups which can regulate balance in the stomach and intestines, resist aging, prolong the life, facilitate digestion, expel toxin for beauty, and prevent and treat thrombi, constipation and diarrhea. The health care liquid integrates health care and treatment, is very wide in market prospect, and conforms to the existing medical viewpoint of people that the prevention, health care, treatment and recovery are combined.

The invention discloses a method for preparing bacterial cellulose by dynamic and static combination, which is characterized in that: an acetobacter xylinum strain is taken and inoculated into a seed culture medium, is inoculated into a seeding tank for amplification culture for 12 to 36h according to 4 to 16 percent of inoculum concentration after the shaking culture by a shaking table at a temperature of between 25 and 31 DEG C and a rotating speed of between 100 and 180rpm for 12 to 48h, is inoculated into a productive tank to be cultured for 12 to 36h, and is packaged separately and then undergoes tray culture for 3 to 10 days at a culture temperature of between 25 and 31 DEG C to produce a finished product, a crude cellulose membrane. The method has simple process, the output is apparently improved, and the output of the bacterial cellulose produced by the method is high up to 13.6g/L (dry weight).

Comprising a wire mesh structure covered with biocompatible material and, more particularly, a substantially cylindrical expandable endoprosthesis (10) covered with biosynthetic cellulosic membrane for the treatment of arterial stenosis, where such structure is introduced in a mold (11), which is made of a material that is impermeable to liquids and permeable to gases, together with a culture medium (12) inoculated with a cellulose-producing microorganism such as the Acetobacter xylinum, resulting in the formation of a cellulosic membrane juxtaposed to the inner wall of said mold. The membrane can be impregnated to the inner wall of said mold (11). The membrane can be impregnated with therapeutic agents to be locally released during a period of days or weeks. Methods used to mount and deploy the endoprosthesis in the body.

The invention provides bacterial cellulose (BC) producing bacteria and a method for preparing the bacterial cellulose by using the bacterial strain. The bacterial strain is a selected gluconic acid acetobacter xylinum GD-BC-1 with an accession number of CCTCC M 208149. The method is to use the bacterial strain as a fermentation bacterial strain and use glucose as a main fermentation material for fermentation so as to obtain the BC. The preparation method comprises the following steps of: activating a slant bacterial strain, liquid-activating the slant bacterial strain, inoculating the liquid-activated slant bacterial strain in a fermentation culture medium to perform dynamic or static fermentation and extracting products of BC from the fermentation solution. The preparation method uses the bacterial strain of gluconic acid acetobacter xylinum GD-BC-1 with the accession number of CCTCC M 208149 for fermentation so as to produce the BC. The BC can be produced through dynamic or static fermentation in a conical flask with glucose as the main fermentation material, and the yield is high and different methods can be used in different production environment to produce BC.

The invention relates to a method for preparing a cuprous oxide/ bacterial cellulose nano composite material. The preparing method comprises the following steps of: obliquely inoculating acetobacter xylinum strains into culture solution, and culturing the strains to obtain a bacterial cellulose gel film; performing surface activation treatment on the bacterial cellulose gel film in sulfuric acid solution and sodium hypochlorite solution respectively; and sequentially soaking the surface activated bacterial cellulose gel film into Cu(NO3)2 solution and hydroquinone solution to perform reduction reaction to obtain the cuprous oxide/ bacterial cellulose nano composite material. The method has the advantages that: the preparation process is simple; and the prepared composite material keeps the nano structure of cuprous oxide particles, simultaneously has good strength performance, and has wide application prospect on the aspects of electrode devices, catalysts, gas sensitive sensors and the like.

The present invention discloses a preparation method for a biological cellulose composite membrane mask material. In the preparation method, water soluble macromolecular carboxymethyl cellulose is added into a culture medium, and then acetobacter xylinum is switched in for static culturing to obtain a biological cellulose composite membrane. The biological cellulose composite membrane generated after the carboxymethyl cellulose is added has good water retention property and good ventilation property, and the output and the water content of the biological cellulose composite membrane are improved to a certain extent. The biological cellulose composite membrane mask material can be obtained after the generated biological cellulose composite membrane is processed.

The invention discloses a preparation method of a tremella fermentation extract and its application. The preparation method of the tremella fermentation extract comprises the following step: tremella undergoes fermentation cultivation by the use of a strain or its culture solution or its suspension so as to obtain a fermentation product, namely the tremella fermentation extract. The strain is at least one of the following strains: zygosaccharomyces rouxii, pasteur yeast, brewer's yeast, wine yeast, acetobacter xylinum, Lactobacillus delbrueckii subsp. Bulgaricus, Streptococcus thermophilus, Bifidobacterium bifidum, lactococcus lactis and enterococcus faecalis. Through experiments, it proves that the tremella fermentation extract prepared by the method contains no chemical components, can be directly used as a finished product of facial mask or essence or toner, is more natural than other existing products in the market and will not have any negative effect on the skin. In addition, smaller components can be obtained by the preparation method in comparison with a common extraction method and the extract is easier to fully absorb by the skin.

The invention provides an Acetobacter xylinum Y05 strain and a method for preparing nanometer cellulose skin tissue repairing material with the same. The preparation method comprises the following steps that: Acetobacter xylinum Y05 (CCTCC M 207163) preserved on an inclined plane is made into a shake-flask seed and cultivated statically to obtain a nanometer cellulose membrane; and the well-cultivated membrane is separated, purified and then compounded with natural polysaccharide (chitosan, etc.) and protein (collagen, silk fibroin, etc.), so as to prepare the repairing material. The nanometer repairing material has good biocompatibility, flexibility and strength which are similar to human skin, as well as good plasticity and elasticity, can be suitable for various irregular wound surfaces, can keep moisture for a long time, and can be used as a skin substitute and medical dressings for burns, chronic skin ulcers and other skin injuries or defects in clinical practice.

The invention discloses a new method for producing bacterial cellulose. The method comprises the following steps of: accommodating culture medium fermentation liquor in which three strains, namely acetobacter xylinum, acetic acid bacteria and angel yeast are inoculated at the bottom of a pouring fermentation tower which is used for producing vinegar and serves as production equipment by a pouringfermentation method, wherein a culture medium is a mixed solution of stocked rice saccharification liquor and alcoholic fermentation mash; filling corncob and husks in a packing layer on the middle part of the tower, and horizontally laying the husks at the bottom and on the upper part of the corncob, wherein the corncob and the husks are used as thallus attached vectors; lifting the three kinds of strain culture medium fermentation liquor at the bottom of the tower to the top of the tower by using a pump, spraying to the packing layer, returning to the bottom of the tower through the packinglayer, and performing cyclic pouring in the mode at the fermentation temperature of between 28 and 32 DEG C at the frequency of 1 time per 4 hours, wherein the pouring time each time is 1 minute, 12 hours are used as one control cycle, and the total fermentation time is 144 hours; and stopping the pouring fermentation, tearing a bacterial cellulose membrane on the corncob off, washing, and dryingto obtain the bacterial cellulose.

The invention discloses a bacterial cellulose/poly(3,4-ethylenedioxythiophene) nanometer conductive composite material and a preparing method thereof. The method includes: a step of removing impurities of bacterial cellulose fermented by acetobacter xylinum, pretreating to obtain a clean bacterial cellulose raw material, and performing freeze-drying to obtain bacterial cellulose aerogel; a step of adding the bacterial cellulose aerogel into a 3,4-ethylenedioxythiophene solution, dispersing uniformly, and performing ultrasonic processing so as to allow the ethylenedioxythiophene monomer to be fully adsorbed by the bacterial cellulose; a step of adding a solution of ferric chloride anhydrous with the same volume, performing in-situ oxidation polymerization under ultrasonic constant-temperature conditions, and a step of subjecting the obtained crude product to ultrasonic washing with methanol (or ethanol) and deionized water repeatedly, fully dipping the crude product with deionized water, and performing freeze-drying to obtain the nanometer conductive composite material. The nanometer conductive composite material has advantages of low cost, mild reaction, high speed, good biocompatibility and a three-dimensional net structure.

Disclosed is a femented beverage with black tea bacterium as the bacterial and its brewing process, which employs by-product of red date or jujube fabrication as the raw material, and utilizes saccharomycete, Acetobacter xylinum and plant lactic acid bacillus as the bacterial through the procedures of first stage culturing, second stage culturing, fermentation, centrifugation and germ-free filtration.

The invention discloses an air filter type bacterial cellulose mask and a production method thereof. The mask is made of dried bacterial cellulose which is used as a mask frame material. The preparation method comprises the following steps: selecting acetobacter xylinum as a type strain, and culturing the acetobacter xylinum to obtain a bacterial cellulose membrane; and after soaking the membrane in acid liquor and alkali liquor, washing the soaked membrane till the rate of impurities is less than 0.5 percent, soaking the membrane in nano-silver solution, then drying the membrane by adopting a freeze drying mode, and obtaining the air filter type bacterial cellulose mask through compound preparation. The preparation process is simple, the filter performance of the mask can be effectively improved by using the bacterial cellulose as a material, and the bactericidal and antitoxic capacities of the mask are enhanced by soaking in the nano-silver solution; and the mask has the characteristics of low production cost, strong adsorption capacity, high bactericidal activity, long durative bactericidal time and the like, and can effectively reduce resource consumption and protect ecological environment.

The invention provides a bacterial cellulose pervaporation membrane, which is characterized in that the preparation method is as follows: Acetobacter xylinum DT4.2 strain of the active wood fermentation is put into the seed culture fluid and shaking cultured at constant temperature of 25 to 32 DEG C for 8 to 24 hours; the seed solution is put into the fermentation culture solution to be evenly shaken; and then the fermentation culture solution is moved to the container for culturing for 7 to 15 days at the constant temperature of 25 to 32 DEG C; pellicle membrane formed on the surface of the fermentation solution is taken out and then is processed by the alkali liquor with the quality concentration of 1 to 20 percent; the residual alkali liquor in the membrane is washed to neutrality by the deionized water to prepare into the membrane after drying. The invention also discloses the application of the membrane. The preparation method of the membrane has the advantages of simple preparation, good mechanical and chemical property, excellent heat stability, strong hydrophilicity, high separation choice to the water in the organic water solution at lower temperature, long service life; moreover, the membrane material itself can be biodegradation which is a environmental friendly material.

The invention relates to a bacterial cellulose culture method which concretely comprises the steps of: inoculating acetobacter xylinum strain in a culture medium for recovery; after recovery, inoculating 2-20% of acetobacter xylinum strain in the culture medium; culturing the inoculated acetobacter xylinum strain by a shaking bed for 2-3 days under the condition of 100-180 rpm; and transferring the obtained fermentation liquor into a shallow tray and culturing for 2-5 days in a static state to obtain bacterial cellulose, wherein the culture temperature is 26-31 DEG C, the culture ventilation amount is 0.3-0.6vvm, and the culture medium is potato culture solution. The culture method has the characteristics that: potato waste residue obtained after starch production is firstly taken as the raw material of the culture medium, is treated by a simplest method and then is used as the culture medium for culturing the bacterial cellulose; the culture medium is low in cost and high in yield; meanwhile, the problem of recycling the potato waste residue can be solved, and the culture time is short; and in addition, the invention provides an important condition for stably culturing the bacterial cellulose in a large scale.

The invention is a bacterial cellulose acoustic vibration film. The vibration film is characterized in that the manufacturing method of the vibration film comprises the following steps of inoculating Acetobacter xylinum strains into a culture fluid; inoculating the strain liquid to a ferment culture fluid and shaking up after homothermal shake cultivation is conducted for the strains; moving the ferment liquid to a container; and conducting homothermal and static cultivation for a plurality of days until a surface film is formed on the surface of the ferment liquid; or moving the ferment liquid to a shaker; conducting homothermal and static cultivation; processing the bacterial cellulose by using lye with the mass concentration in the range of 1% to 8% after the bacterial cellulose is taken out; washing out the remained lye in the film with deionized water to achieve neutrality; and drying with an oven or heat-pressing, processing and shaping to get the film. The invention has the advantages of simple manufacturing method, good mechanical properties, and high thermal stability. The invention has the basic properties of an excellent acoustic vibration film as well as high rate modulus of elasticity and relatively large dissipation factors. In addition, the invention is durable and environmental friendly as the film can realize self-biodegradation.

The invention discloses a probiotics active health-care fluid for preventing and treating senile dementia and a preparation method of the probiotics active health-care fluid. The probiotics active health-care fluid for preventing and treating senile dementia comprises the following raw materials: photosynthetic bacteria, saccharomyces cerevisiae, lactobacillus, acetobacter xylinum, bifidobacterium, brown sugar, defatted soybean powder, honey, sodium chloride, oligosaccharide and deionized water. By adopting the probiotics active health-care fluid, large amounts of probiotics are compensated, so that the intestinal flora is improved, the intestinal leak syndrome is prevented, and the chemical substances (including heavy metal and other harmful substances) without being digested are prevented from entering the intestinal lining and playing roles in blood circulation; and as a result, the senile dementia caused by heavy metal poisoning can be prevented and treated. The probiotics can generate large amounts of active enzymes by which the peptide produced in the human body can be quickly degraded, and the blood cells generated in the human body can be decreased, so that the senile dementia and the degeneration can be delayed. Compared with the prior art, the probiotics active health-care fluid has the advantages of being high in efficiency and stability, and free from side effects.

The invention relates to an application method of bacteria cellulose for the protection of ancient silk relics, comprising the specific steps as follows: treating the surface of the ancient silk relic to prepare two bacteria cellulose repair agents: wherein, acetobacter xylinum is activated with a slant culture medium to experience fermentation culture to obtain pure cultures which are admixed with a liquid culture medium to obtain the repair agent a, or acetobacter xylinum freeze-dried powders are dissolved with aseptic sugar-containing stroma to admix the dissolution liquid with liquid culture stroma to obtain the repair agent b; spraying an atomizing agent a or b onto the surface of the silk relic for culture to form a layer of cellulose membrane on the surface of the silk relic, and then obtain the well-processed relic after post-treatments. By making use of the characteristics of high strength and high flexibility of the bacteria cellulose generated by the acetobacter xylinum, the invention mixes and atomizes strains and the culture stroma to be dispersed on the surface of the silk relic, and a thin layer of nano-cellulose membrane is formed on the surface of the silk relic after the culture, thus improving the flexibility and mechanical strength of the ancient silk relics.

The invention discloses a preparation method of an aloe polysaccharide/bacterial cellulose composite membrane for a medical dressing. The preparation method comprises the following steps: activating a strain Acetobacter xylinum NUST4.2; preparing a seed solution of the strain Acetobacter xylinum NUST4.2; adding aloe polysaccharide with different concentrations into a fermentation culture medium of the strain Acetobacter xylinum NUST4.2 to carry out dynamic-static combined fermentation production, so as to obtain an aloe polysaccharide/bacterial cellulose composite membrane; purifying the aloe polysaccharide/bacterial cellulose composite membrane; respectively carrying out infrared characterization and scanning electron microscope observation on the structure and morphology of the aloe polysaccharide/bacterial cellulose composite membrane; and carrying out physical performance test on the aloe polysaccharide/bacterial cellulose composite membrane as a medical dressing, so as to show that the requirements of the medical dressing are met. By virtue of the preparation method, more choices are provided for the medical dressing for healing skin wounds, and meanwhile, a positive role for the development of the medical dressing is achieved.

The invention discloses a heavy metal ion adsorption film and a preparation method thereof, and belongs to the technical field of composite materials. The preparation method comprises the following steps: 1, fermenting to produce bacterial cellulose by using acetobacter xylinum; 2, obtaining a bacterial cellulose film and performing purification treatment on the bacterial cellulose film, then performing cationization on the cellulose film by taking 3-chloro-2-hydroxypropyl trimethyl ammonium chloride as an etherifying agent; 3, performing crosslink blending reaction on the cationized bacterial cellulose film and chitosan, so as to obtain a chitosan-bacterial cellulose composite material. The composite material prepared by the invention has a better adsorption effect on heavy metal ions, can effectively remove the heavy metal ions in domestic sewage and industrial wastewater, and can be recycled and reused. The composite material has good application prospect in the field of sewage treatment.

The invention discloses a method for preparing chitosan oligosaccharide/bacterial cellulose sponge. The method comprises the steps of: screening out acetobacter xylinum at the stage with highest activity by means of MTT (Methyl Thiazolyl Tetrazolium Colourimetry); adding the chitosan oligosaccharide into a culture system to prepare a chitosan oligosaccharide/bacterial cellulose compound; rinsing the chitosan oligosaccharide/bacterial cellulose compound using distilled water; adding 1% sodium hydroxide solution into the rinsed compound to boil for 4 hours; soaking the compound at room temperature for 0.5 hour by adding 0.5% acetic acid; flushing the compound 5 times using distilled water; and freezing and drying the above processed compound to prepare the chitosan oligosaccharide/bacterial cellulose sponge. The chitosan oligosaccharide/bacterial cellulose sponge prepared by the invention is in a three-dimensional network structure, high in porosity, and dense and uniform in interspace. Experiments prove that the chitosan oligosaccharide/bacterial cellulose sponge is better in hydroscopicity, moisture permeability and blood coagulation effect in comparison with a traditional wound dressing; cytotoxicity by biocompatibility test is 0-grade; the raw materials are easily available; and the preparation process is simple. The chitosan oligosaccharide/bacterial cellulose sponge prepared by the invention is easy to achieve industrialized production and is good in biocompatibility, and thus can be widely used in the field of biomedical materials.

The invention discloses a preparation method of bacterial cellulose. The preparation method comprises the following steps that (1) preparation of shake flask bacteria: acetobacter xylinum ATCC 700178 is inoculated to a seed culture medium and cultured at the temperature of 28 DEG C to 33 DEG C for 12-24 h, and a first-stage seed solution is obtained; (2) enlarge cultivation of the seed: the first-stage seed solution is inoculated to the seed culture medium and subjected to enlarge cultivation, and a second-stage solution is obtained; (3) fermentation: the second-stage seed solution is inoculated to a fermentation tank filled with a molasses culture medium, the fermentation tank is filled with immobilized media, and fermentation is performed for 6-8 days; (4) extraction: the biological nano-crystalline cellulose is extracted from the fermentation liquor. According to the preparation method of the bacterial cellulose, the yield of the biological nano-crystalline cellulose can be improved greatly, the obtained biological nano-crystalline cellulose product is cotton-shaped or flake-shaped, the fibrous structure is looser, and the degree of polymerization is smaller.

The invention discloses a preparation method for a bacterial cellulose in-situ doped composite material, comprising the steps of preparing a fluid medium, then introducing strain acetobacter xylinum, performing rotary shake culture for 12 to 24 hours at the temperature of 15 to 25 DEG C, performing pinhole filter and adding amino acid chelating ferrous, performing rotary shake culture for 3 to 5 days at the temperature of 15 to 25 DEG C, filtering, washing with deionized water, then cooling and drying, performing high-temperature calcination on a formed composite material by an atmosphere furnace, and taking out after natural cooling to obtain the bacterial cellulose in-situ doped composite material. The material prepared by the invention has the advantages of high-energy density, high electrocatalytic activity, and the like, can be applied to the fields of oxygen reduction reactions, fuel cells, supercapacitors and the like, and the preparation technology is simple and the operation is convenient.

The invention discloses a bacterial cellulose/fabric laminated composite material and a preparation method thereof. The preparation method comprises the following steps: (1) inoculating acetobacter xylinum into a fluid medium for enlarged cultivation; (2) appropriately cutting a fabric and treating with low-temperature oxygen plasmas or aqueous alkali, or through ultraviolet light surface grafting; and sterilizing and then drying the fabric; (3) arranging the medium containing floccules into a culture dish, spreading the treated fabric on bacterium solution, adding the medium containing floccules into the fabric, and arranging in a biochemical incubator until the laminated composite material is formed; and (4) soaking the composite material with deionized water at normal temperature, soaking the composite material with sodium hydroxide solution for two days, and soaking the composite material with deionized water until the composite material is neutral. The composite material is formed at a time, the appetency of the single fabric and the texture is improved, and the water retainability and the transdermal absorption ratio of the fabric are improved; meanwhile, the operation is convenient and simple and the using efficacy is greatly improved.

The invention relates to a biosynthesis method for preparing a bacterial cellulose-inorganic nanoparticle hybrid membrane by using microbes. The first preparation method comprises the following steps of: culturing acetobacter xylinum in a liquid nutrient medium added with titanate dihydroxybis[2-hydroxypropanoato-O1,O2]diammonium, and purifying to prepare a bacterial cellulose-TiO2 nano hybrid membrane; the second preparation method comprises the following steps of: hydrothermally decomposing titanate dihydroxybis[2-hydroxypropanoato-O1,O2]diammonium to synthesize TiO2 colloidal particles, and adding the colloidal particles into a nutrient medium, culturing acetobacter xylinum in the nutrient medium, and purifying to prepare the bacterial cellulose-TiO2 nano hybrid membrane; and the thirdpreparation method comprises the following steps of: culturing acetobacter xylinum in a liquid nutrient medium added with sodium silicate or silicic acid, and purifying to prepare the bacterial cellulose-TiO2 nano hybrid membrane. The method has the advantages that: the preparation method is simple, clean, non-toxic and environmentally-friendly, and can solve the problems of high energy consumption, environmental pollution, cytotoxicity and the like in physical and chemical preparation methods.

The present invention provides engineered composite materials for use in medical treatment of injured or degenerated menisci, cartilage, and bone. The composite materials include a cellulosic layer substantially or completely consisting of β-1→4-glucan units, and a hydrogel layer substantially or completely consisting of copolymers of β-1→2-glucan, β-1→3-glucan, and/or β-1→4-glucan, or mixtures of two or all three of these units. Production of the composite materials is achieved in a single culture milieu, using regulation of oxygen availability to control production of the various units and deposition of the layers by Acetobacter xylinum or other microorganisms that produce extracellular cellulosic material.