15 results about "Acetobacter xylinum" patented technology

The invention relates to a probiotic-bacterial cellulose composite membrane as well as a preparation method and application thereof. The probiotic-bacterial cellulose composite membrane is prepared from lactobacillus plantarum LCC-605 with the preservation number of CCTCC No: M 2016491, a biological membrane, a metabolite and a postbiotic of the metabolite, and the probiotic-bacterial cellulose composite membrane is prepared from lactobacillus plantarum LCC-605 with the preservation number of CCTCC No: M 2016491. The method comprises the following steps: carrying out in-situ co-culture on Acetobacter xylinum and Lactobacillus plantarum LCC-605 to form a Lactobacillus plantarum biofilm based bacterial cellulose membrane and related metabolites of the Lactobacillus plantarum biofilm based bacterial cellulose membrane, and carrying out in-situ co-culture on Acetobacter xylinum and Lactobacillus plantarum LCC-605 to form the Lactobacillus plantarum biofilm based bacterial cellulose membrane. The invention not only provides the bacterial cellulose membrane for encapsulating the probiotics to enhance the survival rate and the stability of the probiotics, but also provides an ideal leavening agent for preparing the fermented black bean milk with excellent texture, high viable count and excellent storage stability.

The invention relates to a preparation method of a bacterial cellulose composite nano-silver biological dressing, and belongs to the technical field of biological material preparation. Enzymatic hydrolysate of bean pulp, alfalfa leaching liquor and tryptone are used as fermentation culture media, acetobacter xylinum seed liquid is added, the bacterial cellulose membrane is obtained, and the content of the bacterial cellulose membrane composite nano-silver biological dressing is limited to 50-99% of water. Comprising chitosan, gamma-polyglutamic acid, beta-glucan, refined water and silver ions. The bacterial cellulose membrane composite nano-silver biological dressing has the effects that the bacterial cellulose membrane composite nano-silver biological dressing is high in mechanical strength, high in water-retaining property, good in biocompatibility, good in exudate absorption capacity and non-toxic, and as the nano-silver biological dressing for wounds, the cellulose composite nano-silver biological dressing can provide a moist environment, is beneficial to rapid cleaning of the wounds, and has a good application prospect. Meanwhile, the antibacterial activity of chitosan, gamma-polyglutamic acid, beta-glucan, silver ions and the like on bacteria is more beneficial to wound healing.

The invention relates to a multifunctional bacterial cellulose gel film and a preparation method and application thereof.The gel film is synthesized by acetobacter xylinum in situ in a fermentation medium containing polymyxin B and panax notoginseng saponins, the gel film is of a three-dimensional network structure, the polymyxin B and the panax notoginseng saponins are wrapped in a bacterial cellulose network, and the polymyxin B and the panax notoginseng saponins are evenly distributed in the bacterial cellulose network. And the fiber diameter of the gel film is 48.68 + / -5 nm. The prepared wound dressing has the wet adhesion characteristic and can effectively promote rapid and scar-free healing of chronic infectious wounds and large-area wounds, so that the method is expected to be widely applied to green synthesis of the wound dressing with antibacterial and wound healing promoting functions, and a new form is provided for use of the pseudo-ginseng powder and the polymyxin B.

The invention belongs to the technical field of organic fertilizer and microbial fertilizer manufacturing, and discloses a liquid compound fertilizer prepared by microbial fermentation of acetobacter xylinum and application thereof, and the liquid compound fertilizer is prepared by the following steps: firstly, performing endophytic fungus in-situ activation on a solid raw material to construct a biological barrier; then controlling the solid-to-liquid ratio to form a wet solid mixture, and spatially limiting the enzymolysis reaction on the surface layer of the particles by using an interface liquid film to construct a gradient structure which is sparse outside and dense inside; according to the method disclosed by the invention, the enzymolysis process is converted from a liquid-phase reaction which depends on time control into a spatial self-limiting solid-phase reaction which is determined by physical parameters, so that the robustness of the process on raw material fluctuation is improved, and the finally obtained bacterium skeleton integrated complex has the advantages of high yield, high yield and the like. And the microstructure has high consistency and continuation stability.

The invention discloses a nano bacterial cellulose wound repair membrane and a preparation method thereof, and relates to the technical field of biomedical materials, the method comprises the following steps: carrying out fermentation culture on acetobacter xylinum to obtain an original bacterial cellulose membrane; immersing into a carboxymethyl chitosan solution for modification treatment, and then cleaning to be neutral; silver nanoparticles are loaded and irradiated by ultraviolet light to be uniformly distributed; and finally, performing vacuum drying and hot-pressing treatment to obtain a finished product. According to the invention, bacterial cellulose and carboxymethyl chitosan are combined and loaded with silver nanoparticles to form a composite material with excellent mechanical properties and a long-acting antibacterial function, and the introduction of carboxymethyl chitosan significantly improves the flexibility and biocompatibility of a bacterial cellulose membrane; meanwhile, the membrane material is endowed with long-acting antibacterial ability through loading of the silver nanoparticles, the mechanical property, flexibility and antibacterial ability of the membrane material can be remarkably improved, and meanwhile, the complexity of the preparation process is optimized.

The application relates to a preparation method of a bacterial cellulose-based multilayer structure functional bionic scaffold, which comprises the following steps: firstly, injecting a biomaterial solution into a silica gel model with a microchannel system to perform freeze drying to obtain a porous biological scaffold; secondly, performing crosslinking treatment on the porous biological scaffold; thirdly, inoculating a culture bacteria solution with a Xylobacter concentration of 10 9 ~ 10 15 individuals / mL on the crosslinking-treated porous biological scaffold, and performing static culture and freeze drying to obtain a bacterial cellulose-based composite scaffold; and finally, adopting a coating solution to coat the bacterial cellulose-based composite scaffold through a microchannel system, and performing demolding treatment to obtain the bacterial cellulose-based multilayer structure functional bionic scaffold. The application provides a functional coating process for the multilayer bionic scaffold by using the shrinkage space of the treated material, the micro-pore channel and the injection method of the patterned drainage network, effectively prevents the penetration of the coating solution, and realizes the perfect solidification of the coating solution on the scaffold.

The invention discloses a preparation method of high-rehydration bacterial cellulose powder, which comprises the following steps: inoculating an acetobacter xylinum seed solution into a fermentation culture medium, carrying out static fermentation to obtain bacterial cellulose, and then mincing, pulping, cleaning, carrying out high-pressure homogenization and spray-drying on the obtained bacterial cellulose to obtain bacterial cellulose powder. Based on an innovative process combining optimized fermentation culture medium formula and whole-course physical treatment, through organic combination of processes of static fermentation, multi-stage mechanical crushing, high-pressure homogenization, spray drying and the like, high-efficiency purification and form reconstruction of bacterial cellulose are realized under the condition that no chemical alkaline agent is used, and the method is suitable for industrial production. Finally, a powder product with excellent rehydration performance, high specific surface area and good functional adaptability is obtained, and a new technical path is opened up for high-valued application of the bacterial cellulose.

The invention relates to a preparation method and application of hydrogel loaded with natural nicotine, and belongs to the technical field of bio-based materials. The method comprises the following steps: inoculating acetobacter xylinum to a tobacco extract liquid serving as a culture medium, and fermenting to obtain bacterial cellulose containing natural nicotine components; the preparation method comprises the following steps: homogenizing and defibrating bacterial cellulose, mixing with a collagen peptide solution, carrying out cross-linking reaction, adding into a calcium chloride solution to obtain nicotine-loaded cellulose gel balls, and further preparing into a nicotine bag. Efficient loading of nicotine in the hydrogel is achieved through a specific process, the prepared hydrogel material has good stability, the nicotine bag nicotine release speed is uniform, and a new technical scheme is provided for controlled release of nicotine.

This invention relates to a recombinant xylosinophilic acid bacterium, its preparation method, and its application. The recombinant xylosinophilic acid bacterium is *Xylostella spp.* (…). Komagataeibacter xylinus This invention relates to recombinant *Acetobacter xylinum* strain 23770-EB, accession number CGMCC 31806. The recombinant *Acetobacter xylinum* strain developed in this invention expresses a fusion enzyme protein with mannose kinase and phosphomannose isomerase activities, significantly improving mannose utilization. Developing mannose-rich biomass culture media from coffee grounds, konjac, and spruce for this strain is of great significance for low-cost bacterial cellulose production and resource recycling.

A PLGA composite myocardial patch for in-situ synthesis of cerium dioxide by bacterial cellulose, a preparation method and application thereof, wherein the preparation method of the PLGA composite myocardial patch for in-situ synthesis of cerium dioxide by bacterial cellulose comprises the following steps: S1, inoculating Acetobacter xylinum seed liquid into a container containing liquid HS culture medium for first culture; S2, adding trivalent cerium salt solution into the container for in-situ synthesis of cerium dioxide nanoparticles and second culture, finally obtaining a bacterial cellulose-cerium dioxide-bacterial cellulose composite substrate; S3, constructing a polylactic acid-glycolic acid copolymer nanofiber layer on the surface of the bacterial cellulose-cerium dioxide-bacterial cellulose composite substrate obtained in S2 through electrospinning, and obtaining the PLGA composite myocardial patch. The PLGA composite myocardial patch has good mechanical matching, cell compatibility, antioxidant capacity and interface bonding force.

The invention relates to the technical field of microorganisms, and particularly discloses an acetobacter xylinum HNNB6-Se capable of resisting selenium and producing red selenium-rich nano cellulose, and the preservation number of the acetobacter xylinum HNNB6-Se is GDMCC (China General Microbiological Culture Collection Center) 66552. The strain is obtained by performing sodium selenite gradient stress domestication on an original strain acetobacter xylinum HNNB6, and can grow in a culture medium containing 1-200 [mu] g / mL of sodium selenite and synthesize the red selenium-rich nano bacterial cellulose. The selenium content in the produced nano cellulose (BC) reaches 50-5000 micrograms per gram by dry weight, and the produced nano cellulose (BC) is in stable red (the chromatic value a * is 0.5-25). The red selenium-rich nano bacterial cellulose has a selenium bioaugmentation function, and has outstanding application value in functional food selenium supplements (such as oral tablets and gel foods), medical materials (such as wound dressings) and beauty makeup products.

The invention discloses a bacterial cellulose / collagen composite active hernia patch material and an in-situ biosynthesis method thereof. The method comprises the following steps: extracting collagen from a collagen extraction raw material to obtain purified collagen sponge, dissolving the collagen sponge in water to obtain a collagen solution, adding an oil phase into the collagen solution, and emulsifying to obtain a collagen-based Pickering emulsion; and then, by taking the collagen Pickering emulsion as a template, forming the bacterial cellulose / collagen composite active hernia patch material in an acetobacter xylinum in-situ fermentation culture manner. The bacterial cellulose / collagen composite active hernia patch material with a macroporous structure on one side and an original compact structure on the other side is provided, the macroporous structure of collagen provides a channel for migration of cells and transportation of metabolites, and the compact structure of bacterial cellulose keeps the original high mechanical strength of bacterial cellulose; therefore, the material is favorable for promoting hernia repair.

The application relates to the technical field of medical wound dressings, in particular to a bacterial cellulose-collagen in-situ composite dressing film and a preparation method thereof. The preparation method comprises the following steps: preparing a collagen solution and a culture medium; using acetoxybacter and candida as a composite seed solution; under sterile conditions, adding the composite seed solution and the collagen solution into the culture medium, carrying out static fermentation at 29+ / -1 DEG C after sealing, and obtaining a composite film; and carrying out purification treatment on the composite film to obtain the bacterial cellulose-collagen in-situ composite dressing film. Through in-situ composite fermentation, the BC nanofiber forms a continuous three-dimensional skeleton, the collagen molecules are uniformly physically entangled between the fibers, there is no chemical crosslinking agent residue, and the problems of uneven collagen loading and easy falling off can be solved.

The invention discloses a preparation method and application of a cement shrinkage-enhancement synergistic regulation material based on bacterial cellulose hydrogel, and relates to the technical field of low-water-binder-ratio cement-based composite materials. The invention aims to solve the contradiction between self-constriction inhibition and degradation of mechanical properties of the traditional internal curing material. The method comprises preparation of a novel cement shrinkage reducing material (BCH) and application of the novel cement shrinkage reducing material in cement, and bacterial cellulose synthesized by fermentation of acetobacter xylinum is purified and crushed to prepare bacterial cellulose hydrogel (BCH). And finally, mixing the BCH with cement with a low water-binder ratio to realize reduction-enhancement synergistic regulation and control of the cement-based material. According to the novel reduction material, the BCH self-assembly structure and the multi-scale pore characteristics of the BCH self-assembly structure are utilized, additional preparation means are not needed, meanwhile, the novel reduction material is not sensitive to the internal environment of the cement-based material, and the synergistic effect of internal maintenance and performance regulation and control is achieved. The method is applied to the field of cement-based composite materials.

The application discloses a preparation method of a functional bacterial cellulose-based nerve conduit with anti-inflammatory and nerve repair promoting functions, and belongs to the field of microbial medical materials and tissue engineering. The application adds polyvinyl alcohol into a characteristic culture medium, co-cultures with Acetobacter xylinum, and adds curcumin in the culture system, to obtain a bacterial cellulose film containing polyvinyl alcohol and curcumin through in-situ synthesis, and then the bacterial cellulose film is purified, freeze-dried, flattened, curled, sutured and shaped to prepare the nerve conduit with a hollow tubular structure. The application utilizes the tackifying and hydrogen bond anchoring effects of polyvinyl alcohol to realize efficient and stable loading of curcumin in the three-dimensional network of bacterial cellulose. The infrared spectrum, X-ray diffraction, animal implantation experiment and cell experiment verify that the nerve conduit has good biocompatibility, significant anti-inflammatory performance and nerve repair promoting function. The method is simple and green, and the prepared nerve conduit has wide application prospects in the field of peripheral nerve injury repair.