4 results about "Acetobacter xylinum" patented technology

This invention relates to a recombinant xylosinophilic acid bacterium, its preparation method, and its application. The recombinant xylosinophilic acid bacterium is *Xylostella spp.* (…). Komagataeibacter xylinus This invention relates to recombinant *Acetobacter xylinum* strain 23770-EB, accession number CGMCC 31806. The recombinant *Acetobacter xylinum* strain developed in this invention expresses a fusion enzyme protein with mannose kinase and phosphomannose isomerase activities, significantly improving mannose utilization. Developing mannose-rich biomass culture media from coffee grounds, konjac, and spruce for this strain is of great significance for low-cost bacterial cellulose production and resource recycling.

A PLGA composite myocardial patch for in-situ synthesis of cerium dioxide by bacterial cellulose, a preparation method and application thereof, wherein the preparation method of the PLGA composite myocardial patch for in-situ synthesis of cerium dioxide by bacterial cellulose comprises the following steps: S1, inoculating Acetobacter xylinum seed liquid into a container containing liquid HS culture medium for first culture; S2, adding trivalent cerium salt solution into the container for in-situ synthesis of cerium dioxide nanoparticles and second culture, finally obtaining a bacterial cellulose-cerium dioxide-bacterial cellulose composite substrate; S3, constructing a polylactic acid-glycolic acid copolymer nanofiber layer on the surface of the bacterial cellulose-cerium dioxide-bacterial cellulose composite substrate obtained in S2 through electrospinning, and obtaining the PLGA composite myocardial patch. The PLGA composite myocardial patch has good mechanical matching, cell compatibility, antioxidant capacity and interface bonding force.

The application relates to the technical field of medical wound dressings, in particular to a bacterial cellulose-collagen in-situ composite dressing film and a preparation method thereof. The preparation method comprises the following steps: preparing a collagen solution and a culture medium; using acetoxybacter and candida as a composite seed solution; under sterile conditions, adding the composite seed solution and the collagen solution into the culture medium, carrying out static fermentation at 29+ / -1 DEG C after sealing, and obtaining a composite film; and carrying out purification treatment on the composite film to obtain the bacterial cellulose-collagen in-situ composite dressing film. Through in-situ composite fermentation, the BC nanofiber forms a continuous three-dimensional skeleton, the collagen molecules are uniformly physically entangled between the fibers, there is no chemical crosslinking agent residue, and the problems of uneven collagen loading and easy falling off can be solved.

The application discloses a preparation method of a functional bacterial cellulose-based nerve conduit with anti-inflammatory and nerve repair promoting functions, and belongs to the field of microbial medical materials and tissue engineering. The application adds polyvinyl alcohol into a characteristic culture medium, co-cultures with Acetobacter xylinum, and adds curcumin in the culture system, to obtain a bacterial cellulose film containing polyvinyl alcohol and curcumin through in-situ synthesis, and then the bacterial cellulose film is purified, freeze-dried, flattened, curled, sutured and shaped to prepare the nerve conduit with a hollow tubular structure. The application utilizes the tackifying and hydrogen bond anchoring effects of polyvinyl alcohol to realize efficient and stable loading of curcumin in the three-dimensional network of bacterial cellulose. The infrared spectrum, X-ray diffraction, animal implantation experiment and cell experiment verify that the nerve conduit has good biocompatibility, significant anti-inflammatory performance and nerve repair promoting function. The method is simple and green, and the prepared nerve conduit has wide application prospects in the field of peripheral nerve injury repair.