Preparation method of water-soluble carbon dot and application of water-soluble carbon dot in microbial fermentation

A technology of water-soluble carbon dots and microbial fermentation, which is applied in the directions of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of difficulty in separation and purification of carbon dots, and achieve the effect of simple preparation process and promotion effect.

Inactive Publication Date: 2017-05-17
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Hydrothermal and solvothermal methods are energy-efficient, environmentally friendly and non-toxic methods for synthesizing carbon dots, and are also the most likely methods for mass production, but there are certain difficulties in the separation and purification of carbon dots
[0004] Fluorescent carbon dots are widely used in cell labeling, biosensors and bioimaging, but there are few reports on the application of carbon dots in microbial fermentation to improve the yield of target products

Method used

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  • Preparation method of water-soluble carbon dot and application of water-soluble carbon dot in microbial fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Wash, remove impurities, dry and pulverize discarded river crab shells, and collect crab shell powder with a size less than 0.22 μm. Put the crab shell powder in a tube furnace, pass nitrogen gas to remove the air for 15 minutes, then raise the temperature to 550°C at 5°C / s, and collect the pyrolysis products after 1h of pyrolysis. The pyrolyzed product was washed under ultrasonication for 30 minutes, and the brownish-yellow solution was collected after centrifugation at 12000 rpm for 30 minutes. The solution is dialyzed to remove small molecular impurities to obtain water-soluble fluorescent carbon dots, and freeze-dried to obtain fluorescent carbon dot crystals with a carbon dot fluorescence quantum yield of 35%. Fluorescent carbon dot crystals are dissolved in deionized water to prepare an aqueous solution of fluorescent carbon dots with a mass concentration of 0.3%.

[0018] The carbon dot aqueous solution is sterilized and added to the Klebsiella fermentation medi...

Embodiment 2

[0020] Put the crab shell powder in a tube furnace, pass nitrogen gas to remove the air for 15 minutes, then raise the temperature to 350°C at 10°C / s, and collect the pyrolysis products after pyrolysis for 5h. The pyrolysis product was washed under ultrasonication for 60 minutes, and the brownish yellow solution was collected after centrifugation at 9000 rpm for 30 minutes. The solution is dialyzed to remove small molecule impurities to obtain water-soluble fluorescent carbon dots, and freeze-dried to obtain fluorescent carbon dot crystals with a carbon dot fluorescence quantum yield of 29%. Fluorescent carbon dot crystals are dissolved in deionized water to prepare an aqueous solution of fluorescent carbon dots with a mass concentration of 0.6%.

[0021] The carbon dot aqueous solution is sterilized and added to the Klebsiella fermentation medium, and the volume ratio of the carbon dot aqueous solution to the fermentation medium is 1:9. After 24 hours of fermentation, the co...

Embodiment 3

[0023] Put the crab shell powder in a tube furnace, pass nitrogen gas to remove the air for 15 minutes, then raise the temperature to 400°C at 7°C / s, and collect the pyrolysis products after pyrolysis for 2 hours. The pyrolyzed product was washed under ultrasonication for 30 minutes, and the brownish-yellow solution was collected after centrifugation at 12000 rpm for 30 minutes. The solution is dialyzed to remove small molecule impurities to obtain water-soluble fluorescent carbon dots, and freeze-dried to obtain fluorescent carbon dot crystals with a carbon dot fluorescence quantum yield of 32%. Fluorescent carbon dot crystals are dissolved in deionized water to prepare an aqueous solution of fluorescent carbon dots with a mass concentration of 0.3%.

[0024] After sterilizing the carbon dot aqueous solution, it was added to the Acetobacter xylinum fermentation medium, and the volume ratio of the carbon dot aqueous solution to the fermentation medium was 1:4. After static fe...

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Abstract

The invention discloses a preparation method of a water-soluble carbon dot and application of the water-soluble carbon dot in microbial fermentation, belonging to the technical field of new energy and new materials. According to the preparation method, river crab shell waste is as the raw material, and the acquired carbon dot fluorescence quantum yield can achieve 35% through a simple thermal cracking process. The prepared water-soluble fluorescence carbon dot has biocompatibility, and has an obvious acceleration effect on the fermentation of pneumonia klebsiella pneumonia, the yield of 1,3-propylene glycol can be improved by 30%. Furthermore, the water-soluble fluorescence carbon dot is applied to the standing fermentation of the acetobacter xylinum, the product bacteria cellulose yield can be improved by 75%. The water-soluble fluorescence carbon dot is applied to the microbial fermentation for the first time, and an obvious promotion effect is achieved, and a new growth factor is provided for the microbial fermentation.

Description

technical field [0001] The invention belongs to the technical field of new energy and new materials, and relates to a preparation method of water-soluble carbon dots and its application in microbial fermentation. Background technique [0002] Carbon dots are fluorescent carbon nanoparticles with a one-dimensional scale of less than 10nm. Since Scrivens et al. discovered carbon dots in the purification of carbon nanotubes in 2004, they have received widespread attention due to their good biocompatibility, simple and easy preparation methods, adjustable luminescence range, and stable luminescence properties. Carbon dots have excellent properties, cheap raw materials and simple preparation methods, and are a new type of ecological environment material. In the past few years, researchers have done a lot of in-depth research on the synthesis, mechanism research and application of carbon dots. In addition, carbon dots have high application value in biological drug delivery, biol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12P19/04C01B32/15B82Y30/00B82Y40/00C12R1/22C12R1/02
CPCB82Y30/00B82Y40/00C12P7/18C12P19/04
Inventor 郭峰郭晓倩柳婷李奕姗朱子含
Owner DALIAN UNIV OF TECH
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