Preparation method for bacterial cellulose in-situ doped composite material

A technology of bacterial cellulose and in-situ doping, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of being easily oxidized into ferric ions, etc., to avoid the inhibition of bacterial growth, Effect of high tensile strength and high bioavailability

Inactive Publication Date: 2016-06-08
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to overcome the inhibition of Acetobacter xylinum by ferric ions when bacterial cellulose is doped in situ in t...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Step 1. According to the raw materials containing 5 grams of glucose, 0.1 grams of citric acid, 0.2 grams of disodium hydrogen phosphate, 0.1 grams of potassium dihydrogen phosphate, 0.5 grams of yeast extract, 0.5 grams of peptone and the balance of water in every 100 milliliters of liquid medium Components and proportioning, get the raw materials of each component, dissolve, mix and adjust the pH to be 6, get 250 milliliters of Erlenmeyer flasks, add 125 milliliters of liquid medium in each Erlenmeyer flask, at a pressure of 103 kPa Sterilize for 20 minutes under the condition of steam pressure and temperature of 120°C to obtain a liquid culture medium;

[0030] Step 2, insert the bacterial species Acetobacter xylinum into the liquid medium, and cultivate it for 18 hours at a temperature of 25° C. and a rotation speed of 100 rpm under the condition of rotating shock; use a pinhole filter to add 1 One milliliter of glycine chelated ferrous solution, cultivated for 3 da...

Embodiment 2

[0034] Step 1. According to the raw materials containing 5 grams of glucose, 0.1 grams of citric acid, 0.2 grams of disodium hydrogen phosphate, 0.1 grams of potassium dihydrogen phosphate, 0.5 grams of yeast extract, 0.5 grams of peptone and the balance of water in every 100 milliliters of liquid medium Components and proportioning, get the raw materials of each component, dissolve, mix and adjust the pH to be 6, get 250 milliliters of Erlenmeyer flasks, add 125 milliliters of liquid medium in each Erlenmeyer flask, at a pressure of 103 kPa Sterilize for 20 minutes under the condition of steam pressure and temperature of 120°C to obtain a liquid culture medium;

[0035]Step 2, insert the bacterial species Acetobacter xylinum into the liquid medium, and cultivate it for 18 hours at a temperature of 25° C. and a rotating speed of 100 rpm under the condition of rotating shock; use a pinhole filter to add 2 One milliliter of glycine chelated ferrous solution, cultivated for 3 day...

Embodiment 3

[0039] Step 1. According to the raw materials containing 5 grams of glucose, 0.1 grams of citric acid, 0.2 grams of disodium hydrogen phosphate, 0.1 grams of potassium dihydrogen phosphate, 0.5 grams of yeast extract, 0.5 grams of peptone and the balance of water in every 100 milliliters of liquid medium Components and proportioning, get the raw materials of each component, dissolve, mix and adjust the pH to be 6, get 250 milliliters of Erlenmeyer flasks, add 125 milliliters of liquid medium in each Erlenmeyer flask, at a pressure of 103 kPa Sterilize for 20 minutes under the condition of steam pressure and temperature of 120°C to obtain a liquid culture medium;

[0040] Step 2, insert the bacterial species Acetobacter xylinum into the liquid medium, and cultivate it for 12 hours at a temperature of 25° C. and a rotation speed of 100 rpm under the condition of rotating shock; use a pinhole filter to add 2 One milliliter of glycine chelated ferrous solution, cultivated for 3 da...

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Abstract

The invention discloses a preparation method for a bacterial cellulose in-situ doped composite material, comprising the steps of preparing a fluid medium, then introducing strain acetobacter xylinum, performing rotary shake culture for 12 to 24 hours at the temperature of 15 to 25 DEG C, performing pinhole filter and adding amino acid chelating ferrous, performing rotary shake culture for 3 to 5 days at the temperature of 15 to 25 DEG C, filtering, washing with deionized water, then cooling and drying, performing high-temperature calcination on a formed composite material by an atmosphere furnace, and taking out after natural cooling to obtain the bacterial cellulose in-situ doped composite material. The material prepared by the invention has the advantages of high-energy density, high electrocatalytic activity, and the like, can be applied to the fields of oxygen reduction reactions, fuel cells, supercapacitors and the like, and the preparation technology is simple and the operation is convenient.

Description

technical field [0001] The invention belongs to the field of preparation of doping materials, and in particular relates to a preparation method of bacterial cellulose doping composite materials in situ. Background technique [0002] Bacterial cellulose is a natural fiber produced through a microbial fermentation process, which is different from synthetic fibers required by some complex equipment and large factories, and is generally used in papermaking, food and electronics industries. The production of bacterial cellulose only needs some raw materials, such as glucose, fructose, sucrose, chitosan, etc., and these raw materials are renewable energy, which can be obtained from plants on land and crustaceans in the ocean. In recent years, bacterial cellulose, as a new type of biological nanomaterial, has attracted widespread attention due to its advantages such as low price, high mechanical strength, high specific surface area, sufficient porosity, and abundant sources. Bacte...

Claims

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Application Information

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IPC IPC(8): C08L1/02C08K5/00C12P19/04C12R1/02
CPCC08K5/0091C08L2201/06C12P19/04C08L1/02
Inventor 竹文坤段涛姚卫棠雷佳
Owner SOUTHWEAT UNIV OF SCI & TECH
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