2results about How to "Does not cause immune rejection" patented technology

The invention relates to an in-situ osteochondral regeneration and repair scaffold and a preparation method thereof, the in-situ osteochondral regeneration and repair scaffold prepared by the method comprises a cartilage layer, a connecting layer and a subchondral bone layer, the porosity of the cartilage layer is not less than 85%, the Young modulus is 6-13KPa, and the aperture is 130-140 [mu] m; the porosity of the connecting layer is not lower than 95%, the Young modulus is 4-19 KPa, and the aperture is 50-120 [mu] m; the porosity of the subchondral bone layer is not lower than 95%, the Young modulus is 4-19 KPa, and the aperture is 300-400 microns. The in-situ osteochondral regeneration and repair scaffold prepared by the method has good biocompatibility, is beneficial to attachment, migration and proliferation of cartilage cells and bone marrow mesenchymal stem cells, does not cause immunological rejection, provides a better scaffold environment for repair after osteochondral implantation, and has a good application prospect in the field of in-situ osteochondral regeneration and repair, and experiments prove that the in-situ osteochondral regeneration and repair scaffold has a good application prospect in the field of in-situ osteochondral regeneration and repair. The in-situ osteochondral regeneration and repair scaffold does not affect cell migration; animal experiments prove that the in-situ osteochondral regeneration and repair scaffold has a good repair effect on rat osteochondral defects.

This invention belongs to the field of genetic engineering technology, specifically relating to a recombinant type I collagen, its preparation method, and its applications. Its amino acid sequence is shown in SEQ ID NO.1. The recombinant type I collagen of this invention is based on a short amino acid sequence from natural type I collagen, with the integrin recognition sequence GFPGER added as a repeating unit, repeated multiple times, with a repetition count of 6. The recombinant type I collagen exhibits extremely good hydrophilicity and stability, high adhesion, and does not cause immune rejection when applied to the human body. It can be used in the preparation of tissue engineering materials such as bone implants and bone repair scaffolds.