Preparation method of lymphoma-targeted blood platelet targeting drug loading system carrying and loading anti-tumor drug and connecting CD22 monoclonal antibody

An anti-tumor drug, platelet technology, applied in anti-tumor drugs, drug combinations, pharmaceutical formulations and other directions, can solve the problem of high efficacy of tumor cells, achieve broad application prospects, reduce drug side effects, and have rich effects

Inactive Publication Date: 2017-09-08
NANJING DRUM TOWER HOSPITAL
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, in this work, we propose the use of CD22 mAb-linked platelets as a novel drug-targeted delivery system for the treatment of B-cell lymphoma, which has abundant content, good biocompatibility, high drug loading rate, active targeting It has the advantages of good sex, high curative effect on tumor cells and little damage to normal tissues. There is no relevant research report on this aspect.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of lymphoma-targeted blood platelet targeting drug loading system carrying and loading anti-tumor drug and connecting CD22 monoclonal antibody
  • Preparation method of lymphoma-targeted blood platelet targeting drug loading system carrying and loading anti-tumor drug and connecting CD22 monoclonal antibody
  • Preparation method of lymphoma-targeted blood platelet targeting drug loading system carrying and loading anti-tumor drug and connecting CD22 monoclonal antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A preparation method of a platelet-targeted drug delivery system carrying daunorubicin linked to CD22 monoclonal antibody, the method comprising the following steps:

[0042] Step 1: Add 3.2% sodium citrate solution by weight to the obtained whole blood at room temperature of 20±5°C for anticoagulation, and the volume ratio of whole blood to sodium citrate solution is 9:1 After centrifugation at 1200rpm for 5min, platelet-rich plasma was obtained;

[0043] Step 2: The platelet-rich plasma was centrifuged at 3600rpm for 10min, and washed three times with PBS buffer to make it no longer contain plasma and other blood components, discard the supernatant to obtain washed platelets, and control the platelet count to (2.5-3.0)×10 5 / ul.

[0044] The third step: 500 μl of the resuspension of the washed platelets and 10 μl of MBS reagent were shaken and reacted in a light-proof environment at 37±0.5°C for 1 hour, and centrifuged at 3600rpm for 3 times, each time for 8 minutes,...

Embodiment 2

[0054] A preparation method of a platelet drug-loading system carrying cyclophosphamide linked to CD22 monoclonal antibody, the method comprising the following steps:

[0055] Step 1: Add 4% sodium citrate solution by weight to the obtained whole blood at room temperature of 20±5°C for anticoagulation, and the volume ratio of whole blood to sodium citrate solution is 10:1 , after centrifugation at 1200rpm for 8min, platelet-rich plasma was obtained;

[0056] Step 2: The platelet-rich plasma was centrifuged at 3600rpm for 12min, and washed three times with PBS buffer to make it no longer contain plasma and other blood components. The supernatant was discarded to obtain washed platelets, and the platelet count was controlled to be (2.5-3.0)×10 5 / ul.

[0057] The third step: 500 μl of the resuspension of the washed platelets and 10 μl of MBS reagent were shaken and reacted in a light-proof environment at 37±0.5°C for 1.5 hours, and centrifuged at 3600rpm for 2 times, each time ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of a lymphoma-targeted blood platelet targeting drug loading system which carries and loads a CD22 monoclonal antibody and an anti-tumor drug. The preparation method comprises the following steps: firstly deeply centrifuging and washing blood platelet-enriched blood plasma obtained through whole blood anticoagulation and centrifugation, obtaining washed blood platelets, treating a maleimide group with the surface modified with a pointing sulfhydryl by adopting an MBS reagent, and then reacting with an anti-CD22 monoclonal antibody subjected to sulfhydrylation by virtue of a Traut's reagent, so that the blood platelets which connect the CD22 monoclonal antibody and target lymphoma cells are obtained; and then carrying out mixed incubation on the blood platelets and the anti-tumor drug, centrifuging, and dispersing, so that the target drug loading system is obtained finally. The drug loading system has the advantages of wide source, high drug loading ratio, good biocompatibility, active and accurate targeting, long cycling time and the like, the anti-tumor effect of the drug can be enhanced, and the toxicity on normal tissues is also reduced, so that toxic and side effects of a chemotherapy drug are alleviated, and the drug loading system is significant for improving the life quality and medical quality of a patient with lymphoma.

Description

technical field [0001] The invention belongs to the field of biopharmaceutical carrier preparation, and in particular relates to a preparation method for carrying antitumor drugs by using platelets connected with CD22 monoclonal antibody targeting lymphoma cells as targeted drug carriers. Background technique [0002] Malignant lymphoma is a group of malignant tumors originating in lymphatic tissue, which can be divided into two categories according to pathological and clinical features: Hodgkin's lymphoma (HL) and non-Hodgkin's lymphoma (NHL), the latter accounting for 90% %above. The incidence of NHL has increased dramatically in recent years. Among them, B-cell lymphoma is a group of heterogeneous tumors associated with the monoclonal expansion of malignant B cells, accounting for about 85% of adult NHL. B-cell lymphomas of highly heterogeneous hematological malignancies exhibit distinct clinical and biological features. Despite progress in response to treatment, the 5-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/68A61K31/704A61K31/675A61P35/00
CPCA61K31/675A61K31/704
Inventor 许佩佩左华芹江颖周荣富欧阳建陈兵
Owner NANJING DRUM TOWER HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap