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Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through compound enzymatic hydrolysis of feta protein

A technology of casein and compound enzymolysis, which is applied in the field of enzymatic preparation of ACE inhibitory peptides, to achieve complete hydrolysis, high inhibitory activity, and mild properties

Inactive Publication Date: 2013-07-24
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the microbial fermentation method, the enzymatic reaction is fast, and the preparation of ACE inhibitory peptides has a high inhibitory activity, and the raw materials are widely used, mainly including milk protein, plant protein and animal protein. However, at present, milk-derived ACE inhibitor peptides are mainly made of cow milk. , has not been involved in goat milk

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Step 1: Mix and dissolve goat milk powder and water according to the mass ratio of 1:9, cool the obtained reconstituted goat milk to 4°C, use a high-speed centrifuge to centrifuge at 6400 rpm for 15 minutes, remove fat, and obtain skim milk;

[0017] Step 2: heat the obtained skim milk in a constant temperature water bath at 35°C, stir with a magnetic heating stirrer at a stirring speed of 800 rpm, add HCl solution dropwise while stirring, so that the pH of the skim milk solution reaches 4.6, casein For precipitation, use a high-speed centrifuge to centrifuge at 6400rpm for 15min, discard the supernatant, freeze-dry the precipitated casein, pre-freeze at -40°C for 24h, and then transfer to a freeze dryer at -55°C Freeze-dried for 24 hours to obtain freeze-dried casein powder. It was determined that when the skim milk was at 35°C, pH 4.6, and stirring speed 800 rpm, the casein yield was the highest, reaching 9.23%.

Embodiment 2

[0019] Step 1: Mix and dissolve goat milk powder and water according to the mass ratio of 1:9, cool the obtained reconstituted goat milk to 4°C, use a high-speed centrifuge to centrifuge at 6400 rpm for 15 minutes, remove fat, and obtain skim milk;

[0020] Step 2: heat the obtained skim milk in a constant temperature water bath at 45°C, stir with a magnetic heating stirrer at a stirring speed of 800 rpm, add HCl solution dropwise while stirring, so that the pH of the skim milk solution reaches 4.5, casein For precipitation, use a high-speed centrifuge to centrifuge at 6400rpm for 15min, discard the supernatant, freeze-dry the precipitated casein, pre-freeze at -40°C for 18h, and then transfer to a freeze dryer at -55°C Freeze-dried for 24 hours to obtain freeze-dried casein powder. It was determined that when the skim milk was at 45°C, pH 4.5, and stirring speed 800 rpm, the casein yield was the highest, reaching 11.88%;

[0021] Step 3: Make 100ml of casein solution with a m...

Embodiment 3

[0024] Step 1: Mix and dissolve goat milk powder and water according to the mass ratio of 1:9, cool the obtained reconstituted goat milk to 4°C, use a high-speed centrifuge to centrifuge at 6400 rpm for 15 minutes, remove fat, and obtain skim milk;

[0025] Step 2: heat the obtained skim milk in a constant temperature water bath at 45°C, stir with a magnetic heating stirrer at a stirring speed of 800 rpm, add HCl solution dropwise while stirring, so that the pH of the skim milk solution reaches 4.5, casein For precipitation, use a high-speed centrifuge to centrifuge at 6400rpm for 15min, discard the supernatant, freeze-dry the precipitated casein, pre-freeze at -40°C for 12h, and then transfer to a freeze dryer at -55°C Freeze-dried for 24 hours to obtain freeze-dried casein powder. It was determined that when the skim milk was at 45°C, pH 4.5, and stirring speed 800 rpm, the casein yield was the highest, reaching 11.88%;

[0026] Step 3: Make 100 ml of casein solution with a ...

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Abstract

The invention provides a method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through compound enzymatic hydrolysis of feta protein. The method comprises the following steps of: centrifuging and degreasing recovered goat milk taken as a raw material, preparing casein by an isoelectric precipitation method, carrying out compound hydrolysis on the casein through neutral protease and trypsase under the conditions of specific temperature and pH, optimizing the hydrolysis condition, treating the hydrolytic process with ultrasound waves to obtain small-molecule ACE inhibitory peptides and determining the activity of the small-molecule ACE inhibitory peptides. The inhibitory proportion of an ACE inhibitory peptide product prepared by the method can reach above 80%; the ACE inhibitory peptide product has small molecular mass, can be absorbed by a human body in the form of complete peptide fragments and cannot cause the immunological rejection of the human body; and the ACE inhibitory peptide product has an obvious antihypertensive effect on hypertensive, has no effect on people with normal blood pressure and has the characteristics of little dosage, no toxic or side effect, mild property and strong physiological function.

Description

technical field [0001] The invention relates to the technical field of enzymatic preparation of ACE inhibitory peptides, in particular to a method for preparing ACE inhibitory peptides by compound enzymatic hydrolysis of goat milk casein. Background technique [0002] At present, the prevalence of hypertension in the Chinese population is increasing, with 2 out of every 10 adults suffering from hypertension. It is estimated that there are at least 200 million hypertensive patients nationwide, but the awareness rate, treatment rate and control rate of hypertension are low. Hypertension is a major risk factor for the morbidity and death of stroke and coronary heart disease (CHD). Controlling hypertension can prevent the morbidity and death of cardiovascular and cerebrovascular diseases. [0003] At present, there are mainly 6 kinds of antihypertensive drugs commonly used in the treatment of hypertension, namely diuretics, α-receptor antagonists, calcium channel blockers, angio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 陈合王娟舒国伟
Owner SHAANXI UNIV OF SCI & TECH
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