102 results about "Angiotensin-converting enzyme" patented technology

The invention provides a spirulina-derived ACE (angiotensin converting enzyme) inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of marine bioactive peptides. Spirulina phycobiliprotein is used for preparing the ACE inhibitory peptide of the spirulina source, five kinds of small molecule polypeptides including YATY, RYVTY, YVTY, KAYF and SPSWY are obtained through co-screening, the five kinds of polypeptides all have good ACE inhibitory activity, and the RYVTY activity is the highest. The spirulina-derived polypeptide prepared by the preparation method disclosed by the invention has high hydrolysis degree and high ACE inhibitory activity, can be applied to the fields of food, health care products, medicines and the like, and has a wide application prospect.

The invention discloses a grape seed protein peptide with ACE inhibitory activity and a preparation method thereof, and belongs to the field of comprehensive utilization of wine fermentation byproducts. The invention provides a grape seed protein peptide with ACE (Angiotensin Converting Enzyme) inhibitory activity. The grape seed protein peptide is FFYPL, SGMFPFP or WFFPGPR. The grape seed protein peptide disclosed by the invention can be used for medicines, health-care products or dietary supplements, or can be used as a food base material to be added into common foods such as drinks, dairy products and the like; as used for prevention or auxiliary treatment of hypertensive patients or people suffering from chronic metabolic comprehensive disorder diseases with diseases such as obesity and heart and cerebral vessels, the product has a wide application prospect in the fields of functional foods, medicines, health care products and the like.

The embodiment of the invention discloses a goat milk-derived ACE (angiotensin converting enzyme) inhibitory peptide monomer as well as a preparation method and application thereof, relates to the technical field of protein separation, and aims to improve a preparation source and preparation efficiency of a food-borne protein-derived ACE inhibitory peptide. The preparation method comprises the following steps: dissolving sheep milk casein in water, and carrying out heating treatment, enzymolysis treatment and freeze drying treatment to obtain a sheep milk source zymolyte; preparing the goat milk source zymolyte into a solution with the concentration of 145mg / mL-155mg / mL, passing through a 0.45 mu m filter membrane, and carrying out primary separation by adopting preparative high performance liquid chromatography to obtain a product Y; preparing the Y product into a solution with the concentration of 20 mg / mL-30 mg / mL, passing through a 0.45 mu m filter membrane, and carrying out secondary separation by adopting preparative high performance liquid chromatography to obtain a Y-X product; preparing the Y-X product into a solution with the concentration of 5mg / mL-15mg / mL, passing through a 0.45 mu m filter membrane, and carrying out re-separation preparation by using a C18 hydrophilic preparative column and preparative high performance liquid chromatography to obtain a product M; the product M is prepared into a solution with the concentration of 5 mg / mL-15 mg / mL, the solution passes through a 0.22-micron filter membrane, after re-separation preparation is conducted through RP-HPLC, rotary evaporation concentration treatment and freeze drying treatment are conducted, and a product F is obtained.

The invention provides earthworm protein peptide as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: drying and crushing earthworms to obtain earthworm powder, adding the earthworm powder into water, adding immobilized compound enzyme for enzymolysis, carrying out magnet separation, adjusting the pH value of enzymatic hydrolysate for precipitation, collecting solids, adding the solids into water, adding a purifying agent for adsorption separation, adding the solids into a deep eutectic solvent, heating for desorption, carrying out magnet separation, carrying out ultrafiltration on residual liquid, and collecting protein peptide to obtain the earthworm protein peptide. The immobilized compound enzyme is prepared by immobilizing modified snail protease and alkaline protease on chitosan modified MOF / magnetic microspheres; the purifying agent is a passivated angiotensin converting enzyme immobilized magnetic agar ball. The high-activity earthworm protein peptide has the advantages of simple preparation method, high purity, good inhibition effect on angiotensin converting enzyme, safety, high efficiency, no side effect, good antihypertensive effect on hypertensive patients, good patient compliance, and wide application prospect.

The invention discloses a mulberry leaf antihypertensive active peptide as well as a preparation method and application thereof. According to the method, mulberry leaves are used as raw materials to prepare mulberry leaf albumin, enzymolysis parameters of enzymolysis time, pH, enzyme-substrate ratio and substrate concentration are optimized through a single factor experiment and an orthogonal experiment, and optimal enzymolysis conditions for preparing the ACE inhibitory peptide crude product are provided; the high-activity ACE inhibitory peptide is identified through further purification, polypeptide sequence determination and virtual screening, the ACE inhibitory peptide has extremely high solubility and good ACE inhibitory activity, and the IC50 values of the two peptides VPSCFDLTGK and RLPDFHGL with the highest activity are 8.23 [mu] mol / L (8.765 [mu] g / mL) and 23.01 [mu] mol / L (23.58 [mu] g / mL) respectively. The active peptide can be used as an angiotensin converting enzyme inhibitory peptide, and achieves the purpose of reducing blood pressure by inhibiting ACE activity.

The invention relates to an in-vitro detection reagent, in particular to a reagent and a kit for determining angiotensin converting enzyme and a preparation method of the reagent and the kit. The reagent comprises a substrate and an enzyme activity regulator, the substrate can be catalyzed by angiotensin converting enzyme in a to-be-detected sample to react and generate a detectable signal, the strength of the detectable signal is related to the activity of the angiotensin converting enzyme in the sample, the enzyme activity regulator comprises at least one of three metal ions, namely Zn < 2 + >, Co < 2 + > and Mn < 2 + >, and the concentration of the enzyme activity regulator is more than 10 times of the physiological concentration of metal ions in serum. By optimizing the reagent formula, the detection reaction rate is stabilized, and the analysis sensitivity, precision and accuracy are improved.

The application discloses a polypeptide-based anti-fouling electrochemical sensor and a construction method and application thereof. In the application, cysteine is used as an anchoring end, an alpha-helix structure is formed by inserting polyproline to give a supporting effect to the whole peptide, then angiotensin converting enzyme inhibitory peptide (KSRE) and alpha-glucosidase inhibitory peptide (EKDER) are used as branches to prepare the anti-fouling polypeptide. The anti-fouling polypeptide has a better anti-fouling effect than other anti-fouling polypeptides, and the working electrode is modified by the anti-fouling polypeptide to prepare an anti-fouling electrochemical aptamer sensor for detecting AFB1, so that the anti-pollutant interference effect of the sensor can be greatly improved, the accuracy and sensitivity of AFB1 detection can be improved, and the anti-fouling electrochemical aptamer sensor has a wide application prospect.

The invention discloses an ACE (angiotensin converting enzyme) inhibitory peptide with antihypertensive activity and application thereof, and belongs to the technical field of active peptide biology, amino acid sequences of the ACE inhibitory peptide are EYPVK and SYPVK, the two peptides are obtained through module replacement design, the ACE inhibitory activity of the two peptides is remarkably improved compared with that of an original peptide LYPVK, IC50 values are respectively reduced to 14.82 + / -0.82 mu M and 12.83 + / -0.79 mu M from 117.14 + / -1.40 mu M, and the inhibitory activity is improved by 13.5% and 25.1%. Molecular simulation analysis shows that the interaction force between the two and ACE is stronger, and a potential modular effect exists between sequences. Besides, the polypeptide has remarkable blood pressure lowering activity on human umbilical vein endothelial cells, plays a role by regulating release of NO and ET-1, has the activity superior to that of original peptides, has the advantages of good digestive enzyme stability, small molecular weight and good water solubility, and is suitable for developing related products for lowering blood pressure.

The present invention relates to repurposing the use of angiotensin Converting Enzyme Inhibitors (ACEI) and Angiotensin II Receptor Antagonists (ARAs) to prevent and / or treat gastrointestinal complications associated with Diabetes mellitus.

The application discloses selenium-containing chickpea peptides, a preparation method thereof and application of the selenium-containing chickpea peptides in preparation of blood pressure lowering drugs, and belongs to the technical field of bioactive peptides. Selenium-enriched Bacillus natto is used to ferment chickpeas to obtain selenium-containing chickpea protein hydrolysate, and then fermentation conditions are optimized and quantitative peptidomics technology is used to obtain selenium-containing chickpea peptides PQM and PQML. Experiments prove that the selenium-containing chickpea peptides have good in-vivo stability, bioavailability and blood pressure lowering activity, have an effect of inhibiting angiotensin converting enzyme (ACE) activity, and have potential for preventing or treating hypertension.

The present disclosure relates to pharmaceutical formulations of angiotensin converting enzyme inhibitor and / or diuretic, particularly stable oral pharmaceutical formulations comprising angiotensin converting enzyme inhibitor and / or diuretic with one or more pharmaceutically acceptable excipients for the treatment of hypertension, coronary artery disease, heart failure, acute myocardial infarction and other related cardiovascular diseases.

The invention belongs to the technical field of biological detection, and discloses a method for simultaneously detecting large fragment deletion of ACE (Angiotensin Converting Enzyme) and CYP2D6 genes. The kit provided by the invention can simultaneously amplify a plurality of target fragments (such as CYP2D6 gene deletion long fragments and ACE gene deletion short fragments) with obvious size difference under the same system, so that the experimental operation steps are reduced by half, and the detection time is obviously shortened; the reagent cost is saved, and the use amount of an electrophoresis reagent is reduced by half; operation links are reduced, and the risk of sample confusion is reduced.

The invention discloses a functional beverage based on a jerusalem artichoke component and a preparation method thereof, and belongs to the technical field of functional beverages, and the functional beverage is prepared from the following raw materials in parts by weight: 90-110 parts of jerusalem artichoke, 40-60 parts of bitter gourd, 20-40 parts of hawthorn, 40-60 parts of celery stem, 0.5-1.5 parts of ground cinnamon, 2-4 parts of sugar substitute and 300-500 parts of water. The inulin delays glucose absorption, the balsam pear glycoside promotes sugar utilization, the cinnamon polyphenol enhances insulin sensitivity to achieve sugar metabolism regulation, the jerusalem artichoke short-chain fatty acid inhibits liver cholesterol synthesis, and the hawthorn flavone promotes lipid decomposition to achieve lipid metabolism improvement. The celery potassium-sodium pump is balanced, and the inulin reduces the activity of angiotensin converting enzyme to regulate the blood pressure, so that the functional beverage can regulate the three highs.

Angiotensin-converting enzyme 2 (ACE2) has been identified as a specific receptor for severe acute respiratory syndrome coronavirus (SARS-CoV-1) and several beta-group coronaviruses, including SARS-CoV-2, the causative agent of the recent global pandemic COVID-19, and the low-pathogenic coronavirus HCoV-NL63, a member of the alpha-coronavirus group. The viral spike protein (S) of the viral envelope has been identified as a viral receptor that binds to ACE2 and initiates the viral replication cycle. The present invention provides ACE2 and its mutant or variant forms, as well as viral or non-viral vectors thereof. Methods for treating viral infections in human subjects using such mutant or variant forms are also provided.

The invention discloses a preparation method of spirulina functional polypeptide. The polypeptide provided by the invention has an amino acid sequence shown as a sequence 1 in a sequence table. The functional polypeptide (YSDITRPG) found from spirulina is a purely natural, non-toxic and harmless microbial source substance, has a remarkable ACE (angiotensin converting enzyme) inhibition effect and a colon cancer cell inhibition effect, can be used as an antihypertensive component, can also be used as an active component of a colon cancer medicine, is non-toxic, meets the requirements of medicinal preparation development, and has a wide application prospect. Meanwhile, the product can be easily added into various health-care foods as a functional factor, and has a good market prospect in medicine and food industries.

Disclosed are a peptide, an antibody, or an antigen-binding fragment thereof, which specifically binds to an ACE2 (angiotensin-converting enzyme 2) receptor, and a composition for preventing SARS-CoV-2, the composition comprising the same. The peptide includes at least one peptide sequence selected from a group consisting of [SEQ ID NO: 1] GHPVNSVLLDF, [SEQ ID NO: 2] GHPRVNVGGDF, [SEQ ID NO: 3] GVLGPRLLIDY and [SEQ ID NO: 4] DGPINRTTIDY.

The invention belongs to the field of microbial fermentation, and particularly relates to a method for fermenting celery by lactobacillus plantarum. The lactobacillus plantarum is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36518, and the preservation date is November 06, 2025. The lactobacillus plantarum has acid resistance, bile salt resistance, artificial gastrointestinal fluid tolerance and angiotensin converting enzyme inhibitory activity. The invention discloses application of lactobacillus plantarum in preparation of celery fermented products. The contents of total phenols and total flavonoids in the celery juice fermented by lactobacillus plantarum are greatly increased, and the angiotensin converting enzyme inhibition rate, the DPPH clearance rate and the ABTS clearance rate of the celery leaf fermented juice are obviously increased compared with those of the celery leaf fermented juice without fermentation. According to the method, the polyphenol content and the biological activity of the celery juice are improved through metabolism, and the celery juice has the potential of resisting oxidation and reducing blood pressure and has the prospect of being developed into functional fermented food.

The invention discloses a preparation method and application of an edible grass leaf source active peptide with ACE (angiotensin converting enzyme) inhibitory activity, and belongs to the technical field of biological active peptides. The preparation method comprises the following steps: firstly, extracting azolla filliculoidas protein from azolla filliculoidas powder by adopting an ultrasonic-assisted alkali-solution and acid-isolation method; dissolving a certain amount of rumex hanus powder in distilled water, adjusting the pH value of the solution, carrying out water bath preheating, and adding neutral protease for enzymolysis; and carrying out ultrafiltration and DEAE Sepharose FF anion gel column separation on the obtained enzymolysis product to obtain the azolla filliculoidas source active peptide with efficient angiotensin converting enzyme inhibitory activity. The preparation method is simple in process, mild in condition and easy to popularize, the obtained active peptide has wide application prospects in development of functional food, health care products and antihypertensive drugs, and effective utilization and value improvement of azolla filliculoidas protein resources are facilitated.

The present invention belongs to the technical field of active peptide preparation, and specifically relates to an angiotensin I converting enzyme inhibitory peptide and its application. The present invention uses Yinghong No. 9 black tea residue as raw material, and utilizes enzymatic hydrolysis, ultrafiltration, high performance liquid chromatography separation and purification, mass spectrometry, etc. to screen out angiotensin I converting enzyme inhibitory peptides from 59 peptide segments. The molecular docking results show that the inhibitory peptide can bind to multiple amino acid residues in the S1, S2 and S1′ active pockets of ACE, and it is mainly hydrogen bonds, metal ion binding and hydrophobic interactions that play the most important role in stabilizing the docking complex and inhibiting ACE activity. In addition, the EA.hy926 cell model was used to find that the inhibitory peptide can play an active role by promoting the release of NO and inhibiting the secretion of ET‑1. The inhibitory peptide is expected to be used as a potential antihypertensive drug or functional food ingredient.

The present invention discloses a betaine composition having a blood pressure lowering effect and a preparation method thereof, belonging to the technical field of pharmaceutical compositions. The preparation method includes: preparing a tablet core, preparing a primary coating solution, preparing a secondary coating solution, primary coating, and secondary coating; the preparation of the primary coating solution includes: preparing a filler and primary mixing; the preparation of the secondary coating solution includes: preparing a supramolecular composition and secondary mixing; the betaine composition prepared by the present invention can avoid the problem of too low blood pressure after the use of the prepared betaine composition by controlling the release rate of the angiotensin converting enzyme inhibitor. The prepared betaine mixture has good storage stability, high qualified rate, and small difference in the content of the angiotensin converting enzyme inhibitor among the prepared betaine mixtures.

The present disclosure relates to the use of angiotensin-converting enzyme and / or angiotensin-converting enzyme 2 as a fecal biomarker for the diagnosis of a disease or disorder related to the renin-angiotensin-aldosterone system, and also to the use of angiotensin-converting enzyme and angiotensin-converting enzyme 2 isoforms as fecal biomarkers for the detection of dysbiosis.

The application provides a polypeptide with angiotensin converting enzyme inhibitory activity and a preparation method and application thereof, and relates to the technical field of active peptide preparation. The abalone is pulverized, a complex enzyme is added for primary enzymolysis, and then a specific protease mixture containing prolyl endopeptidase, chymotrypsin and protease K is added for two-step enzymolysis, so that the obtained polypeptide solution has high ACE inhibitory activity. The obtained polypeptide is analyzed and screened to obtain polypeptides with amino acid sequences as shown in SEQ ID NO. 1-5. The polypeptide has high ACE inhibitory activity, and the IC 50 0.05mg / mL-0.40mg / mL, and therefore can play a blood pressure lowering effect as an angiotensin converting enzyme inhibitor.

Provided herein are compositions suitable for treating chemically induced eye damage comprising (a) a nonsteroidal anti-inflammatory drug (NSAID), (b) a histone deacetylase (HDAC) inhibitor and (c) an angiotensin converting enzyme (ACE) inhibitor and, optionally, (d) a water-soluble vitamin. Methods of using provided compositions to treat ocular toxicity and corneal damage following exposure to chemical agents (e.g., mustard gas) are also provided.

This invention discloses a mulberry leaf antihypertensive active peptide, its preparation method, and its application. Using mulberry leaves as raw material, this invention prepares mulberry leaf albumin. Enzymatic hydrolysis parameters, including hydrolysis time, pH, enzyme-to-substrate ratio, and substrate concentration, were optimized through single-factor and orthogonal experiments, providing optimal hydrolysis conditions for preparing crude ACE-inhibiting peptides. Further purification, peptide sequencing, and virtual screening identified highly active ACE-inhibiting peptides with strong solubility and good ACE-inhibiting activity. The two peptides with the highest activity, VPSCFDLTGK and RLPDFHGL, showed the highest IC50 values. 50 The values ​​were 8.23 ​​μmol / L (8.765 μg / mL) and 23.01 μmol / L (23.58 μg / mL), respectively. These bioactive peptides can act as angiotensin-converting enzyme inhibitory peptides, thereby lowering blood pressure by inhibiting ACE activity.

The invention provides an industrial hempseed meal fermentation product for reducing blood pressure as well as a preparation method and application thereof, and belongs to the technical field of deep processing of industrial hempseed meal. The method for preparing the industrial hempseed meal fermentation product for reducing blood pressure comprises the following steps: carrying out alkali dissolution and acid precipitation on industrial hempseed meal to obtain industrial hempseed protein, and mixing the industrial hempseed protein and water according to a solid-liquid ratio of 1g: (20-30) mL to obtain a fermentation culture medium; and inoculating a lactobacillus plantarum seed solution into the fermentation culture medium, fermenting at 35-40 DEG C for 65-72 hours to obtain a fermentation solution, centrifuging, and taking a supernatant to obtain the industrial hempseed meal fermentation product. The industrial hempseed meal leavening prepared by the method disclosed by the invention has an obvious function of inhibiting the activity of angiotensin converting enzyme in vitro, shows higher antihypertensive activity in a hypertensive animal in-vivo experiment, and can influence the concentration of a plurality of substances related to the antihypertensive activity.

The invention discloses lactobacillus helveticus IMAU16360 with ACE (Angiotensin Converting Enzyme) inhibitory activity and application of the lactobacillus helveticus IMAU16360 in fermented milk, and belongs to the field of microorganisms. The lactobacillus helveticus IMAU16360 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36567, the preservation time is November 11, 2025, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The commercial leavening agent PYS-010 and the lactobacillus helveticus IMAU16360 are compounded according to the ratio of 1: 1, the pH and acidity change trend of the compounded group in the storage period is consistent with that of a control group, the compounded group is fused into an existing process system, and an existing production line does not need to be replaced; the elasticity and viscosity of a single strain fermented product are improved, so that the product is fuller in taste; on the premise that the basic taste is not influenced, the fermented milk has ACE inhibitory activity; meanwhile, a relatively high level of viable count is maintained, and good market value is achieved.

The invention discloses a cow milk clear source small molecule peptide, the amino acid sequence of which is Trp-Thr-Pro-Tyr-Arg (WTPYR), and the molecular weight of which is 721.80 Da. The small molecule peptide disclosed by the invention has angiotensin converting enzyme (ACE) inhibitory activity, can be applied to preparation of preparations for preventing and treating hypertension, provides effective active ingredients for developing foods and products with antihypertensive efficacy and without side effects, and has a good application prospect. And the small molecule peptide has the characteristics of simple preparation and safety, and is suitable for industrial production and market popularization and application.

Functionalized nanoparticles for inhibiting or preventing pathogen infections (e.g., viral or bacterial infections, such as coronavirus infections) are described. The nanoparticles comprise a biodegradable polymer core and a lipid coating layer that is functionalized with a pathogen-binding receptor (e.g., an angiotensin-converting enzyme 2 (ACE2) receptor protein) and / or a pathogen-binding antibody or an antigen-binding fragment thereof (e.g., a virus-binding antibody or an antigen-binding fragment thereof). The nanoparticles are further functionalized by a phagocyte-specific ligand, e.g., a phosphatidylserine-containing lipid included in the lipid coating layer, to promote clearance of nanoparticle-bound pathogen. Methods of using the nanoparticles to treat or prevent pathogen infections (e.g., coronavirus infections) are also described.

The invention provides a method for preparing inhalable targeted gold nanoparticles for accelerating pulmonary delivery and treating pulmonary inflammation. The method comprises the following steps: preparing plasmids; carrying out plasmid transformation; carrying out protein expression induction; carrying out protein purification; and the gold nanoparticles (Au (at) PEG-RBD NP) combined with the spikes RBD are prepared. Nanoparticles (NP) in air accumulate more readily in lungs with lipopolysaccharide-induced acute respiratory distress syndrome (ARDS) than in healthy lungs and enter alveolar epithelial cells that express angiotensin converting enzyme (ACE) 2 and L-SIGN (both are Spike receptors activated in ARDS hamsters). The nanoparticles (NP) treat tissue damage, oxidative stress, and inflammation more effectively than corticosteroids, and do not remain gold or create toxicity in the major organs one year after inhalation. The gold core can be used for inhibiting p38 alpha mitogen activated protein kinase and polo-like kinase 3. Similar therapeutic effects have been observed in hamsters suffering from hydrochloric acid induced ARDS. This self-therapeutic nanoparticle (NP), when combined with biomimetic and non-invasive delivery, provides a safe, efficient, and targeted treatment for lung inflammation.

The invention belongs to the technical field of development of plant-derived bioactive peptides, and discloses a preparation method and application of a moringa seed antihypertensive polypeptide. Moringa seed polypeptide with the activity of inhibiting angiotensin converting enzyme (ACE) is separated and purified from moringa seed crude protein, nine moringa seed ACE inhibitory peptides with brand-new sequences and the effect of reducing blood pressure are finally obtained through structural identification, activity screening and solid-phase synthesis, sequence modification is conducted on polypeptide PLVRRAIY with the highest activity, and the trypsin enzymolysis resistance of the polypeptide PLVRRAIY is improved. Molecular level and animal experiments show that both the original peptide PLVRRAIY and the modified peptide PPVQQAIY have significant ACE inhibitory activity and antihypertensive effect, the trypsin degradation resistance of the modified peptide in the gastrointestinal tract environment is enhanced, the stability is significantly improved, and a new resource is provided for development of natural antihypertensive polypeptide drugs.