130 results about "Angiotensin-converting enzyme" patented technology

The invention belongs to the field of bioactive peptides. The invention provides an angiotensin converting enzyme inhibitory peptide as well as a screening method and application thereof. The amino acid sequence of the inhibitory peptide is GKGLW and / or GDGLKW. According to the screening method of the ACE inhibitory peptide, the soybean protein is taken as a raw material, and the ACE inhibitory peptide is prepared and screened by adopting a targeted alkaline protease and chymotrypsin double-enzymolysis technology, so that rapid screening of the ACE inhibitory peptide is realized, and an experimental basis is provided for the structure-function relationship of the ACE inhibitory peptide. The inhibitory peptide provided by the invention has strong antihypertensive activity, high safety and no toxic or side effect. Digestive tract stability determination shows that the inhibitory peptide has good gastrointestinal tract stability in gastrointestinal tract digestion, and can still keep good activity after entering blood.

The invention discloses preparation and application of lotus seed protein peptide with ACE (angiotensin converting enzyme) inhibitory activity, and belongs to the technical field of food-borne active peptide development. According to the ACE inhibitory peptide WVPRYFPF and the preparation method thereof, the ACE inhibitory peptide is rapidly screened by utilizing a polypeptiomics and molecular docking technology, the defects of time consumption and labor consumption caused by step-by-step separation and purification are avoided, the obtained ACE inhibitory peptide WVPRYFPF has extremely high ACE inhibitory activity, IC50 of the ACE inhibitory peptide WVPRYFPF is 16.7 mu M, and the ACE inhibitory peptide WVPRYFPF can be used for assisting or replacing hypertension drugs. The lotus seed protein peptide disclosed by the invention has the characteristic of inhibiting ACE enzyme activity in vitro, and animal experiments prove that the protein peptide can also play a role in lowering blood pressure in a spontaneous hypertension rat (SHR).

The present application provides a soybean antihypertensive peptide and its screening method and application, which belongs to the field of polypeptides. The soybean antihypertensive peptide provided in the present application includes one or more of soybean antihypertensive peptide I with a molecular weight of less than 1000Da, soybean antihypertensive peptide II with a molecular weight of less than 1000Da, and soybean antihypertensive peptide III with a molecular weight of less than 1000Da; wherein, the soybean antihypertensive peptide I with a molecular weight of less than 1000Da is prepared by nattokinase enzymatic hydrolysis of soybean protein isolate, the soybean antihypertensive peptide III with a molecular weight of less than 1000Da is prepared by Bacillus subtilis liquid fermentation of soybean protein hydrolysate, and the soybean antihypertensive peptide III with a molecular weight of less than 1000Da is prepared by Bacillus subtilis solid fermentation of soybeans. The soybean antihypertensive peptide of the present application is a plant-derived antihypertensive peptide with high angiotensin converting enzyme inhibition rate and good antihypertensive effect. And it has been verified by animal experiments that the soybean antihypertensive peptide of the present application can significantly reduce the heart rate, diastolic blood pressure and systolic blood pressure of rats, and has less toxic and side effects.

The invention provides a spirulina-derived ACE (angiotensin converting enzyme) inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of marine bioactive peptides. Spirulina phycobiliprotein is used for preparing the ACE inhibitory peptide of the spirulina source, five kinds of small molecule polypeptides including YATY, RYVTY, YVTY, KAYF and SPSWY are obtained through co-screening, the five kinds of polypeptides all have good ACE inhibitory activity, and the RYVTY activity is the highest. The spirulina-derived polypeptide prepared by the preparation method disclosed by the invention has high hydrolysis degree and high ACE inhibitory activity, can be applied to the fields of food, health care products, medicines and the like, and has a wide application prospect.

Provided are a novel sodium alginate lyase AlyB, a preparation method therefor and the use thereof. The main cracking product of the novel sodium alginate lyase AlyB is a monosaccharide, which has the effects of resisting oxidation, resisting viruses and inhibiting an angiotensin converting enzyme, and thus can be applied to the fields of cosmetics, medicines, healthcare foods and the like.

The invention discloses a grape seed protein peptide with ACE inhibitory activity and a preparation method thereof, and belongs to the field of comprehensive utilization of wine fermentation byproducts. The invention provides a grape seed protein peptide with ACE (Angiotensin Converting Enzyme) inhibitory activity. The grape seed protein peptide is FFYPL, SGMFPFP or WFFPGPR. The grape seed protein peptide disclosed by the invention can be used for medicines, health-care products or dietary supplements, or can be used as a food base material to be added into common foods such as drinks, dairy products and the like; as used for prevention or auxiliary treatment of hypertensive patients or people suffering from chronic metabolic comprehensive disorder diseases with diseases such as obesity and heart and cerebral vessels, the product has a wide application prospect in the fields of functional foods, medicines, health care products and the like.

The invention discloses a goat milk bioactive polypeptide with antioxidation and angiotensin converting enzyme inhibition activity, and preparation and application thereof, and belongs to the field of biological polypeptides, and a preparation method and application thereof. In-vitro tests prove that the polypeptide has an excellent antioxidant effect, can remarkably reduce blood pressure and is beneficial to prevention and treatment of cardiovascular diseases, the interaction mechanism of the polypeptide and ACE is defined by using a molecular docking technology, scientific basis and targets are provided for research and development of new drugs, and the polypeptide has potential physiological functions such as antioxidant activity and ACE inhibitory activity and has a broad application prospect. The method is suitable for functional food and medicine development and has a wide application prospect.

An immunotherapeutic protein and methods of use and production thereof are disclosed, wherein the immunotherapeutic protein comprises, for example, a cell surface receptor polypeptide which is a cellular entry receptor for the entry of a virus into a host cell, which is linked to a polypeptide comprising an Fc region component. When the cell surface receptor polypeptide is an angiotensin converting enzyme 2 (ACE2) polypeptide or a fragment thereof, the immunotherapeutic protein is capable of binding to a coronavirus spike protein (S protein) and may be useful as an antiviral agent for the prevention or treatment of a coronavirus infection. The Fc region component may comprise an amino acid substitution at the position corresponding to H429 of the amino acid sequence of the human IgG1 heavy chain polypeptide which may enable the production of soluble oligomeric forms or the assembly of oligomeric forms from soluble monomeric forms upon binding to an S protein of a coronavirus (i.e. as present on a virion or the surface of virus-infected cells).

The embodiment of the invention discloses a goat milk-derived ACE (angiotensin converting enzyme) inhibitory peptide monomer as well as a preparation method and application thereof, relates to the technical field of protein separation, and aims to improve a preparation source and preparation efficiency of a food-borne protein-derived ACE inhibitory peptide. The preparation method comprises the following steps: dissolving sheep milk casein in water, and carrying out heating treatment, enzymolysis treatment and freeze drying treatment to obtain a sheep milk source zymolyte; preparing the goat milk source zymolyte into a solution with the concentration of 145mg / mL-155mg / mL, passing through a 0.45 mu m filter membrane, and carrying out primary separation by adopting preparative high performance liquid chromatography to obtain a product Y; preparing the Y product into a solution with the concentration of 20 mg / mL-30 mg / mL, passing through a 0.45 mu m filter membrane, and carrying out secondary separation by adopting preparative high performance liquid chromatography to obtain a Y-X product; preparing the Y-X product into a solution with the concentration of 5mg / mL-15mg / mL, passing through a 0.45 mu m filter membrane, and carrying out re-separation preparation by using a C18 hydrophilic preparative column and preparative high performance liquid chromatography to obtain a product M; the product M is prepared into a solution with the concentration of 5 mg / mL-15 mg / mL, the solution passes through a 0.22-micron filter membrane, after re-separation preparation is conducted through RP-HPLC, rotary evaporation concentration treatment and freeze drying treatment are conducted, and a product F is obtained.

The invention provides earthworm protein peptide as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: drying and crushing earthworms to obtain earthworm powder, adding the earthworm powder into water, adding immobilized compound enzyme for enzymolysis, carrying out magnet separation, adjusting the pH value of enzymatic hydrolysate for precipitation, collecting solids, adding the solids into water, adding a purifying agent for adsorption separation, adding the solids into a deep eutectic solvent, heating for desorption, carrying out magnet separation, carrying out ultrafiltration on residual liquid, and collecting protein peptide to obtain the earthworm protein peptide. The immobilized compound enzyme is prepared by immobilizing modified snail protease and alkaline protease on chitosan modified MOF / magnetic microspheres; the purifying agent is a passivated angiotensin converting enzyme immobilized magnetic agar ball. The high-activity earthworm protein peptide has the advantages of simple preparation method, high purity, good inhibition effect on angiotensin converting enzyme, safety, high efficiency, no side effect, good antihypertensive effect on hypertensive patients, good patient compliance, and wide application prospect.

The invention relates to the technical field of biology, in particular to antioxidative peptide and ACE (angiotensin converting enzyme) inhibitory peptide from tuna as well as a preparation method and application of the antioxidative peptide and the ACE inhibitory peptide. The amino acid sequences of the polypeptide provided by the invention are as shown in SEQ ID NO.1-6. The preparation method comprises the following steps: by taking processing waste tuna skin as a raw material, carrying out enzymolysis to obtain tuna skin collagen, then carrying out liquid chromatography-tandem mass spectrometry identification and molecular docking screening on a tuna skin collagen zymolyte, and extracting to obtain polypeptide with antioxidant activity and polypeptide capable of effectively inhibiting ACE (Angiotensin Converting Enzyme) activity. According to the method, the protein extraction step is simplified; meanwhile, on one hand, the deep processing level of the tuna industry can be improved, high-value utilization is achieved, and on the other hand, the bearing pressure of the environment on the processing waste can be reduced.

The invention discloses a mulberry leaf antihypertensive active peptide as well as a preparation method and application thereof. According to the method, mulberry leaves are used as raw materials to prepare mulberry leaf albumin, enzymolysis parameters of enzymolysis time, pH, enzyme-substrate ratio and substrate concentration are optimized through a single factor experiment and an orthogonal experiment, and optimal enzymolysis conditions for preparing the ACE inhibitory peptide crude product are provided; the high-activity ACE inhibitory peptide is identified through further purification, polypeptide sequence determination and virtual screening, the ACE inhibitory peptide has extremely high solubility and good ACE inhibitory activity, and the IC50 values of the two peptides VPSCFDLTGK and RLPDFHGL with the highest activity are 8.23 [mu] mol / L (8.765 [mu] g / mL) and 23.01 [mu] mol / L (23.58 [mu] g / mL) respectively. The active peptide can be used as an angiotensin converting enzyme inhibitory peptide, and achieves the purpose of reducing blood pressure by inhibiting ACE activity.

The invention discloses a drug delivery system for metabolism regulation of specific immune cells and a preparation method and application thereof.The drug delivery system is of a double-layer vesicle structure and comprises inner vesicles and outer vesicles, the inner vesicles are polymer vesicles formed by self-assembly of diblock polymers PEI-b-PLA or PEI-b-PLA, and the outer vesicles are polymer vesicles formed by self-assembly of diblock polymers PEI-b-PLA or PEI-b-PLA. The outer wall of the inner-layer vesicle is positively charged and is loaded with a therapeutic gene for immunoregulation of Arginase-1, the surface of the inner-layer vesicle is modified by an antibody targeting an MDSC cell surface marker, and a biguanide therapeutic drug is loaded in the inner-layer vesicle; the outer-layer vesicle is a liposome-like vesicle modified by angiotensin converting enzyme substrate polypeptide-PEG (Polyethylene Glycol) which is disintegrated in a targeted manner. The drug delivery system designed by the invention can effectively avoid influence on immune cells outside a focus, and further performs specific drug delivery and function regulation and control on immune cells such as immune microenvironment MDSC in the focus, so that effective immunotherapy of placental diseases becomes possible.

The invention relates to an in-vitro detection reagent, in particular to a reagent and a kit for determining angiotensin converting enzyme and a preparation method of the reagent and the kit. The reagent comprises a substrate and an enzyme activity regulator, the substrate can be catalyzed by angiotensin converting enzyme in a to-be-detected sample to react and generate a detectable signal, the strength of the detectable signal is related to the activity of the angiotensin converting enzyme in the sample, the enzyme activity regulator comprises at least one of three metal ions, namely Zn < 2 + >, Co < 2 + > and Mn < 2 + >, and the concentration of the enzyme activity regulator is more than 10 times of the physiological concentration of metal ions in serum. By optimizing the reagent formula, the detection reaction rate is stabilized, and the analysis sensitivity, precision and accuracy are improved.

The application discloses a polypeptide-based anti-fouling electrochemical sensor and a construction method and application thereof. In the application, cysteine is used as an anchoring end, an alpha-helix structure is formed by inserting polyproline to give a supporting effect to the whole peptide, then angiotensin converting enzyme inhibitory peptide (KSRE) and alpha-glucosidase inhibitory peptide (EKDER) are used as branches to prepare the anti-fouling polypeptide. The anti-fouling polypeptide has a better anti-fouling effect than other anti-fouling polypeptides, and the working electrode is modified by the anti-fouling polypeptide to prepare an anti-fouling electrochemical aptamer sensor for detecting AFB1, so that the anti-pollutant interference effect of the sensor can be greatly improved, the accuracy and sensitivity of AFB1 detection can be improved, and the anti-fouling electrochemical aptamer sensor has a wide application prospect.

The invention discloses an ACE (angiotensin converting enzyme) inhibitory peptide with antihypertensive activity and application thereof, and belongs to the technical field of active peptide biology, amino acid sequences of the ACE inhibitory peptide are EYPVK and SYPVK, the two peptides are obtained through module replacement design, the ACE inhibitory activity of the two peptides is remarkably improved compared with that of an original peptide LYPVK, IC50 values are respectively reduced to 14.82 + / -0.82 mu M and 12.83 + / -0.79 mu M from 117.14 + / -1.40 mu M, and the inhibitory activity is improved by 13.5% and 25.1%. Molecular simulation analysis shows that the interaction force between the two and ACE is stronger, and a potential modular effect exists between sequences. Besides, the polypeptide has remarkable blood pressure lowering activity on human umbilical vein endothelial cells, plays a role by regulating release of NO and ET-1, has the activity superior to that of original peptides, has the advantages of good digestive enzyme stability, small molecular weight and good water solubility, and is suitable for developing related products for lowering blood pressure.

The present invention relates to repurposing the use of angiotensin Converting Enzyme Inhibitors (ACEI) and Angiotensin II Receptor Antagonists (ARAs) to prevent and / or treat gastrointestinal complications associated with Diabetes mellitus.

The application discloses selenium-containing chickpea peptides, a preparation method thereof and application of the selenium-containing chickpea peptides in preparation of blood pressure lowering drugs, and belongs to the technical field of bioactive peptides. Selenium-enriched Bacillus natto is used to ferment chickpeas to obtain selenium-containing chickpea protein hydrolysate, and then fermentation conditions are optimized and quantitative peptidomics technology is used to obtain selenium-containing chickpea peptides PQM and PQML. Experiments prove that the selenium-containing chickpea peptides have good in-vivo stability, bioavailability and blood pressure lowering activity, have an effect of inhibiting angiotensin converting enzyme (ACE) activity, and have potential for preventing or treating hypertension.

The present disclosure relates to pharmaceutical formulations of angiotensin converting enzyme inhibitor and / or diuretic, particularly stable oral pharmaceutical formulations comprising angiotensin converting enzyme inhibitor and / or diuretic with one or more pharmaceutically acceptable excipients for the treatment of hypertension, coronary artery disease, heart failure, acute myocardial infarction and other related cardiovascular diseases.

The invention belongs to the technical field of biological detection, and discloses a method for simultaneously detecting large fragment deletion of ACE (Angiotensin Converting Enzyme) and CYP2D6 genes. The kit provided by the invention can simultaneously amplify a plurality of target fragments (such as CYP2D6 gene deletion long fragments and ACE gene deletion short fragments) with obvious size difference under the same system, so that the experimental operation steps are reduced by half, and the detection time is obviously shortened; the reagent cost is saved, and the use amount of an electrophoresis reagent is reduced by half; operation links are reduced, and the risk of sample confusion is reduced.

The invention discloses a functional beverage based on a jerusalem artichoke component and a preparation method thereof, and belongs to the technical field of functional beverages, and the functional beverage is prepared from the following raw materials in parts by weight: 90-110 parts of jerusalem artichoke, 40-60 parts of bitter gourd, 20-40 parts of hawthorn, 40-60 parts of celery stem, 0.5-1.5 parts of ground cinnamon, 2-4 parts of sugar substitute and 300-500 parts of water. The inulin delays glucose absorption, the balsam pear glycoside promotes sugar utilization, the cinnamon polyphenol enhances insulin sensitivity to achieve sugar metabolism regulation, the jerusalem artichoke short-chain fatty acid inhibits liver cholesterol synthesis, and the hawthorn flavone promotes lipid decomposition to achieve lipid metabolism improvement. The celery potassium-sodium pump is balanced, and the inulin reduces the activity of angiotensin converting enzyme to regulate the blood pressure, so that the functional beverage can regulate the three highs.

The invention discloses a polypeptide-based anti-fouling electrochemical sensor as well as a construction method and application thereof. Cysteine is used as an anchoring end, polyproline is inserted to form an alpha-helical structure to provide a supporting effect for the whole peptide, and then angiotensin converting enzyme inhibitory peptide (KSRE) and alpha-glucosidase inhibitory peptide (EKDER) are used as branches to prepare the anti-fouling polypeptide. Compared with other anti-pollution polypeptides, the anti-pollution polypeptide has a better anti-pollution effect, the anti-pollution polypeptide is used for modifying a working electrode and preparing an anti-pollution electrochemical aptamer sensor for detecting AFB1, the anti-pollution interference effect of the sensor can be greatly improved, the accuracy and sensitivity of AFB1 detection can be improved, and the application prospect is wide. Wide application prospects are realized.

Angiotensin-converting enzyme 2 (ACE2) has been identified as a specific receptor for severe acute respiratory syndrome coronavirus (SARS-CoV-1) and several beta-group coronaviruses, including SARS-CoV-2, the causative agent of the recent global pandemic COVID-19, and the low-pathogenic coronavirus HCoV-NL63, a member of the alpha-coronavirus group. The viral spike protein (S) of the viral envelope has been identified as a viral receptor that binds to ACE2 and initiates the viral replication cycle. The present invention provides ACE2 and its mutant or variant forms, as well as viral or non-viral vectors thereof. Methods for treating viral infections in human subjects using such mutant or variant forms are also provided.

The invention discloses a preparation method of spirulina functional polypeptide. The polypeptide provided by the invention has an amino acid sequence shown as a sequence 1 in a sequence table. The functional polypeptide (YSDITRPG) found from spirulina is a purely natural, non-toxic and harmless microbial source substance, has a remarkable ACE (angiotensin converting enzyme) inhibition effect and a colon cancer cell inhibition effect, can be used as an antihypertensive component, can also be used as an active component of a colon cancer medicine, is non-toxic, meets the requirements of medicinal preparation development, and has a wide application prospect. Meanwhile, the product can be easily added into various health-care foods as a functional factor, and has a good market prospect in medicine and food industries.

Disclosed are a peptide, an antibody, or an antigen-binding fragment thereof, which specifically binds to an ACE2 (angiotensin-converting enzyme 2) receptor, and a composition for preventing SARS-CoV-2, the composition comprising the same. The peptide includes at least one peptide sequence selected from a group consisting of [SEQ ID NO: 1] GHPVNSVLLDF, [SEQ ID NO: 2] GHPRVNVGGDF, [SEQ ID NO: 3] GVLGPRLLIDY and [SEQ ID NO: 4] DGPINRTTIDY.

The invention belongs to the field of microbial fermentation, and particularly relates to a method for fermenting celery by lactobacillus plantarum. The lactobacillus plantarum is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36518, and the preservation date is November 06, 2025. The lactobacillus plantarum has acid resistance, bile salt resistance, artificial gastrointestinal fluid tolerance and angiotensin converting enzyme inhibitory activity. The invention discloses application of lactobacillus plantarum in preparation of celery fermented products. The contents of total phenols and total flavonoids in the celery juice fermented by lactobacillus plantarum are greatly increased, and the angiotensin converting enzyme inhibition rate, the DPPH clearance rate and the ABTS clearance rate of the celery leaf fermented juice are obviously increased compared with those of the celery leaf fermented juice without fermentation. According to the method, the polyphenol content and the biological activity of the celery juice are improved through metabolism, and the celery juice has the potential of resisting oxidation and reducing blood pressure and has the prospect of being developed into functional fermented food.

The invention discloses a preparation method and application of an edible grass leaf source active peptide with ACE (angiotensin converting enzyme) inhibitory activity, and belongs to the technical field of biological active peptides. The preparation method comprises the following steps: firstly, extracting azolla filliculoidas protein from azolla filliculoidas powder by adopting an ultrasonic-assisted alkali-solution and acid-isolation method; dissolving a certain amount of rumex hanus powder in distilled water, adjusting the pH value of the solution, carrying out water bath preheating, and adding neutral protease for enzymolysis; and carrying out ultrafiltration and DEAE Sepharose FF anion gel column separation on the obtained enzymolysis product to obtain the azolla filliculoidas source active peptide with efficient angiotensin converting enzyme inhibitory activity. The preparation method is simple in process, mild in condition and easy to popularize, the obtained active peptide has wide application prospects in development of functional food, health care products and antihypertensive drugs, and effective utilization and value improvement of azolla filliculoidas protein resources are facilitated.

Provided is a method for screening a modulator of a viral infection by detecting an interaction of angiotensin converting enzyme 2 (ACE2) and a spike protein with a biosensing platform. Also provided is a compound of formula (I) as a modulator of a viral infection, where R, X, A, R3 to R5, and m are as defined herein, and a method for protecting a subject from a viral infection by administering with the compound.

The present invention discloses a Chinese clam antihypertensive peptide and its application in the preparation of antihypertensive drugs and health foods. The Chinese clam antihypertensive extract is obtained by enzymatic hydrolysis, and the extract is further separated and purified to obtain a polypeptide of a specific sequence, and the amino acid sequence of the antihypertensive peptide is Lys-Asp-Glu-Tyr-Lys-His-Leu-Asn-Phe-Gly-Glu-Val (KDEYKHLNFGEV). The Chinese clam antihypertensive peptide disclosed in the present invention has significant angiotensin converting enzyme (ACE) inhibitory activity. In vitro, it is manifested as temperature, pH, simulated gastrointestinal digestion and angiotensin converting enzyme (ACE) degradation stability. It shows good antihypertensive effect both in vitro and in non-spontaneous hypertensive rats (SHRs), and can be used in health products and medicines related to hypertension treatment.

The invention discloses a recombinant angiotensin converting enzyme inhibitory peptide, a recombinant strain, a preparation method and application of the recombinant angiotensin converting enzyme inhibitory peptide, and belongs to the technical field of genetic engineering and active peptides. The invention discloses a recombinant angiotensin converting enzyme inhibitory peptide. The amino acid sequence of the recombinant angiotensin converting enzyme inhibitory peptide is YWLKP. The ACE inhibitory activity of the recombinant angiotensin converting enzyme inhibitory peptide YWLKP disclosed by the invention is obviously improved compared with that of an original inhibitory peptide LYPVK, and the IC50 value is reduced from 10.6 + / -0.86 mu g / mL to 5.0 + / -0.62 mu g / mL. The recombinant angiotensin converting enzyme inhibitory peptide YWLKP disclosed by the invention has relatively strong intracellular antihypertensive activity, and is beneficial to the application of the recombinant angiotensin converting enzyme inhibitory peptide YWLKP in developing products with antihypertensive efficacy.