29 results about "Alginate lyase" patented technology

The invention provides low-temperature alginate lyase and application thereof, namely alginate lyase with low-temperature catalytic activity, which is separated from marine-derived cold monad bacteria, and the amino acid sequence of the alginate lyase is SEQ ID NO: 1; the amino acid sequence of the alginate lyase after the signal peptide is removed is SEQ ID NO: 3. The algin lyase Alg3889 provided by the invention has the optimal reaction temperature of about 10-16 DEG C, has good temperature stability, and still keeps more than 70% of enzyme activity after being incubated at 0-70 DEG C for 1 hour; the optimum pH value of the strain is 8.0, after the strain is incubated in a buffer system with the pH value of 3.0-10.6 for 12 h, about 90% of the highest enzyme activity can be kept, and the strain has high acid resistance and alkali resistance. The degradation product of the alginate oligosaccharide is alginate oligosaccharide with the polymerization degree of 2-6, and the alginate oligosaccharide has high application value.

The invention relates to algin lyase FsAly7Z with high activity and high thermal stability, which is mainly used for producing brown algae trisaccharide, and an engineering bacterium and application of the algin lyase FsAly7Z. The invention provides an alginate lyase FsAly7Z derived from marine uncultured microorganisms and a preparation method of the alginate lyase FsAly7Z. The FsAly7Z has extremely high thermal stability, and compared with the enzyme activity loss of more than 50% after most enzymes are treated at 40 DEG C for 60 min, the FsAly7Z shows remarkably excellent thermal stability under the same condition, and the half-life period of the FsAly7Z exceeds 24 h; the FsAly7Z can be used for efficiently degrading sodium alginate and a polyG section which is difficult to degrade, and meanwhile, the FsAly7Z also shows the catalytic efficiency superior to that of most similar enzymes; and the content of brown algae trisaccharide in the enzymolysis product is high, so that the subsequent separation and purification process can be simplified, and the production cost can be reduced. In a word, the FsAly7Z is extremely high in thermal stability and excellent in catalytic efficiency, has both economical efficiency and conversion efficiency, can efficiently produce alginate oligosaccharide, is used for analyzing the structure and function association of alginate lyase, and has wide market prospects in the fields of medicine, food industry and the like.

The invention belongs to the technical field of seaweed functional products, and particularly relates to fucoidin as well as a preparation method and application thereof, in particular to application of the fucoidin in food and preparation of medicines for adjuvant treatment of age-related maculopathy. According to the invention, alginate lyase is adopted to carry out enzymolysis on seaweed, and the fucoidin with the molecular weight of 20-60 kDa is obtained after treatment of multi-stage alcohol precipitation, hydrogen peroxide degradation and membrane interception. Experimental results show that the fucoidin has the effect of repairing retinal cells, can effectively improve maculopathy, can be used for preparing related products for treating age-related macular degeneration, and provides safer natural material support for treatment of age-related macular degeneration.

The invention belongs to the field of biological enzyme engineering, and particularly relates to a high-stability alginate lyase mutant and a preparation method thereof. The invention relates to algin lyase mutants AncAlyA1 and AncAlyA2 with high stability. The algin lyase mutants AncAlyA1 and AncAlyA2 are characterized in that coded protein sequences of the algin lyase mutants AncAlyA1 and AncAlyA2 are shown as SEQ ID NO.2 and SEQ ID NO.3. The algin lyase is optimized through an ancestor sequence reconstruction technology, the activity of the optimized enzyme is remarkably improved, and algin degradation can be more efficiently catalyzed. And the heat stability and pH adaptability are enhanced, so that the enzyme keeps activity under wider temperature and pH conditions, the method adapts to complex environmental conditions in industrial production, and the performance reduction caused by environmental fluctuation is reduced. In addition, by optimizing the fermentation process, the enzyme yield is greatly increased, the production cost is reduced, and the economic benefit is improved. Due to the improvements, the technology is superior to the prior art in the aspects of enzyme catalysis efficiency, stability, adaptability and yield, and a better solution is provided for efficient utilization and industrial production of algin.

The invention discloses a fermentation type seaweed granular fertilizer and a fermentation method thereof, and belongs to the technical field of biological fertilizers, plant active substances of enzymolysis seaweed active powder, grain byproducts and organic nutrition of soybean meal are fused, after microbial fermentation, nutrition release is more balanced, robust growth of crops can be promoted, and the yield and quality can be improved; seaweed residues are secondarily utilized through an enzymolysis technology, macromolecular substances are degraded into micromolecular active components through alginate lyase and cellulase, enzymolysis seaweed active powder is prepared, the nutritive value of seaweed is excavated to the maximum extent, the cost of raw materials is reduced, nano zeolite serves as a carrier of microelements, iron ions are reduced and stabilized through potassium borohydride, and then iron ions are reduced and stabilized through potassium borohydride; and then the nitrohumic acid is used for chelating elements such as zinc, iron and boron, and the adsorption performance of the nano zeolite is combined, so that the double stability of the chelated trace elements and the porous carrier is realized.

The application discloses a kind of alginate lyase mutants, coding gene, recombination expression vector and genetically engineered bacteria, belong to genetic engineering technical field.The application uses alginate lyase PpAly7A as starting template, and obtains the mutant with relatively specific activity and thermal stability by site-directed mutagenesis and combination mutation.The relative specific activity of mutant C169A, C66A / C169A, C66A / C169A / C214A, E87Q is increased to 131.42%, 162.89%, 160.97%, 133.82% respectively compared with wild enzyme PpAly7A.The destruction of Glu180-Arg178 salt bond (E180Q) improves thermal stability, and the half-life of the mutant at 50 DEG C is increased to 7.246d compared with wild enzyme PpAly7A, and the site can be used as a new target for subsequent rational design.

The invention provides a homogeneous immune FRET (Fluorescence Resonance Energy Transfer) analysis method and application of alginate, and belongs to the technical field of analytical chemistry and biosensing. The alginate fluorescent probe is prepared by respectively marking FRET donors and receptors on alginate by utilizing the high-specific recognition characteristic of a catalytic inactivated alginate lyase or carbohydrate binding module thereof on the alginate, and the probe is randomly combined on alginate molecules with a linear repetitive skeleton in a novel'sugar-coated haw 'structure. At an appropriate probe: alginate binding ratio, FRET donors and receptors are close to each other and generate FRET signals. The lanthanide complex is used as a fluorescence donor, and short-life background fluorescence interference in a biological sample is effectively filtered out by utilizing the long-life luminescence characteristic of the lanthanide complex. Compared with a conventional polysaccharide analysis method which usually needs a complex pretreatment process and relatively long detection time, the method disclosed by the invention can realize high-sensitivity, high-specificity, rapid and homogeneous immunofluorescence quantitative detection of algal polysaccharides in a biological sample.

The present application relates to a kind of mediated zymolytic enzyme of brown algae and application of double transcription unit recombinant bacteria of oxyprotein and brown algae, belong to genetic engineering technical field, the recombinant engineering bacteria is composed of two transcription units, one transcription unit is GAP-alpha-Vgb-AOX1TT, another transcription unit is AOX-alpha-102C300C-AOX1TT, two transcription units are expressed in tandem in pPICZ alphaA plasmid.The present application also provides the preparation method and application of the recombinant bacteria.The recombinant bacteria of the present application can improve the secretion expression efficiency of brown algae zymolytic enzyme, especially can be expressed in low-oxygen environment.

A C-terminal truncated alginate lyase and its application. This invention belongs to the fields of bioengineering, genetic engineering, and enzyme engineering, and relates to a C-terminal truncated alginate lyase TY2 designed with molecular docking assistance, its encoding gene, recombinant vector, strain, and its application in the preparation of low-polymerization degree alginate oligosaccharides. Using wild-type MhAly6 as the parent, the truncation boundary was determined through structural prediction and molecular docking. A portion of the linker peptide was retained, and the C-terminal GH28 family domain was deleted to obtain the truncated form TY2. TY2 has approximately twice the specific activity of the wild-type enzyme, and even higher residual activity after incubation at 45°C for 1 hour, achieving a synergistic improvement in catalytic activity and thermal stability. + The relative enzyme activity reached 4.3 times that of the control group, which can degrade pretreated Sargassum powder. The product is mainly ΔDP2–ΔDP4 low-polymerization brown algae oligosaccharides, which are suitable for related enzymatic degradation and oligosaccharide preparation.

The application discloses a alginate lyase mutant E87Q, a coding gene, a recombinant expression vector and a genetically engineered bacterium, and belongs to the technical field of genetic engineering. The application takes alginate lyase PpAly7A as a starting template, and a mutant with improved relative specific activity is obtained through site-directed mutagenesis. Compared with the wild enzyme PpAly7A, the mutant E87Q has a relative specific activity increased to 133.82%, and the corresponding site can be used as a new target for subsequent rational design.

The invention discloses an alginate lyase mutant, a coding gene, a recombinant expression vector and a genetically engineered bacterium, and belongs to the technical field of genetic engineering. According to the invention, alginate lyase PpAly7A is taken as a starting template, and a mutant with improved relative specific activity and thermal stability is obtained through site-specific mutagenesis and combined mutagenesis. Compared with a wild enzyme PpAly7A, the relative activity of the mutants C169A, C66A / C169A, C66A / C169A / C214A and E87Q is respectively improved to 131.42%, 162.89%, 160.97% and 133.82%, and the relative activity of the mutants C66A / C169A, C66A / C169A / C214A and E87Q is respectively improved to 131.42%, 162.89%, 160.97% and The thermal stability is improved by damage (E180Q) of a Glu180-Arg178 salt bond, the half-life period of the mutant at 50 DEG C is prolonged to 7.246 d compared with that of a wild enzyme PpAly7A, and the site can be used as a new target for subsequent rational design.

The invention discloses a mucus type pseudomonas aeruginosa bacteriophage separation method based on recombinant alginate lyase, according to the separation method, mucus type pseudomonas aeruginosa bacteriophage is obtained based on recombinant alginate lyase separation, and the amino acid sequence of the recombinant alginate lyase is shown as SEQ ID NO.2. An alginate lyase gene algL in genomic DNA of a pseudomonas aeruginosa standard strain PAO1 is used as a target gene, a recombinant plasmid is constructed, induced expression is completed in escherichia coli, and the recombinant alginate lyase with high purity and high alginate degradation activity is obtained. Based on the recombinant alginate lyase, the bacteriophage which shows a high splitting effect on the mucus type pseudomonas aeruginosa is screened, the problem that the mucus type pseudomonas aeruginosa bacteriophage is difficult to separate is solved, and a technical basis is provided for development of drugs for treating infectious diseases caused by the mucus type pseudomonas aeruginosa.

This invention provides a method for extracting astaxanthin from Haematococcus pluvialis and its application. The method includes the following steps: S1. Raw material pretreatment; S2. Enzymatic hydrolysis with a complex enzyme; S3. Solubilization with a surfactant; S4. Adsorption by iron(III) oxide-cellulose porous microspheres; S5. Magnetic separation and washing; S6. Astaxanthin elution and microsphere recovery; S7. Concentration and drying. This invention utilizes a complex enzyme consisting of cellulase, alginate lyase, and mannanase to synergistically hydrolyze the multilayer polysaccharide structure of thick-walled spores of Haematococcus pluvialis, thereby fully releasing astaxanthin. Furthermore, iron(III) oxide-cellulose porous microspheres with benzyl and octyl surface modifications are used for highly selective adsorption of astaxanthin, combined with magnetic separation technology, thus improving the astaxanthin yield. The extraction process of this invention is carried out under medium-low temperature conditions, and butylated hydroxytoluene is added to the eluent, effectively avoiding thermal degradation, photolysis, and oxidation of astaxanthin, resulting in a high retention rate of antioxidant activity in the obtained astaxanthin.

The application discloses a method for improving salt adaptability of PL7 family alginate lyase. First, surface amino acid, enzyme catalytic site and conservative site are analyzed, then mutation sites are selected according to the analysis, so that positively charged amino acids in the target wild type PL7 family alginate lyase are mutated into negatively charged amino acids or non-charged amino acids; then mutation schemes of each mutation site are selected according to the analysis; if the conservative site analysis result shows that the mutation site exists negatively charged amino acid on other PL7 family alginate lyases, the mutation site is preferentially mutated into glutamic acid or aspartic acid with negative charge; if the site does not exist negatively charged amino acid on other PL7 family alginate lyases, the mutation site is mutated into negatively charged amino acid, and the mutation is preferentially performed into more appearing amino acid. Finally, the salt adaptability is regulated, and the alginate lyase suitable for low salt catalytic environment is obtained.

A truncated alginate lyase with wide temperature adaptability and its applications. This invention belongs to the fields of marine microbial genetic engineering and enzyme engineering, specifically disclosing a truncated alginate lyase, Aly7YΔCBM16ΔCBM32, and its applications. The amino acid sequence of this truncated form is shown in SEQ ID NO.4, and the nucleotide sequence is shown in SEQ ID NO.3. Compared with the wild type, this truncated form exhibits approximately 2-fold increased specific enzyme activity, more than 3-fold extended half-life at 40°C, and a significantly wider temperature and pH adaptability range. Furthermore, after normalization to 100% of the maximum activity of each enzyme, this truncated form retains approximately 78% activity in 1% SDS, demonstrates enhanced catalytic activity for Poly G, and achieves approximately 90% relative enzyme activity even under sodium-free conditions. This truncated form exhibits excellent environmental tolerance and can be used for the efficient preparation of low-polymerization alginate oligosaccharides primarily composed of ΔDP2–ΔDP4.

The application discloses an endolytic alginate lyase AlgC2m and application thereof. The biological enzyme is derived from a Paenibacillus-like bacterium enriched in a seaside soil sample. The application also discloses a recombinant expression vector and a recombinant host containing the alginate lyase AlgC2m gene. The application clones a new alginate lyase gene AlgC2m, which is 1320 bp in size, encodes 439 amino acids, and is classified into the PL-7 family. The similarity of the alginate lyase to alginate lyases in the same family is only 38%. The alginate lyase AlgC2m provided by the application has the function of degrading sodium alginate to prepare alginate oligosaccharide, and the main degradation product is unsaturated alginate disaccharide and trisaccharide, and has certain industrial application value.

The invention relates to the technical field of microorganisms, in particular to a vibrio strain capable of efficiently degrading algin and application of the vibrio strain. The invention relates to a vibrio strain capable of efficiently degrading algin, which is preserved in China General Microbiological Culture Collection Center (CGMCC), the name is (Vibrio sp.) MBPDH9, the preservation number is CGMCC No.35925, the preservation date is September 15, 2025, and the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The vibrio strain provided by the invention can be used for producing the alginate lyase, the product of the alginate lyase for degrading alginate is only brown algae disaccharide, the product specificity is high, and the vibrio strain is beneficial to being applied to industrial production. Meanwhile, the fermentation culture condition of the vibrio strain is further subjected to single-factor experimental research, and precondition research is provided for subsequent large-scale industrial production of the vibrio strain. The vibrio strain provided by the invention can efficiently degrade sargassum horneri in a seawater environment, is a dominant strain for resource utilization of brown algae, and has the advantage of environmental protection.

The invention provides a flight control fertilizer composition for promoting wheat growth and increasing yield as well as a preparation method and application thereof, and relates to the technical field of agricultural fertilizers. The invention relates to a flight control fertilizer composition. The seaweed preservative is prepared from the following raw materials: 240 to 320 parts of an amino acid mixture, 40 to 65 parts of an enzymolysis seaweed extract, 6 to 15 parts of sodium dodecyl benzene sulfonate, 6 to 15 parts of sodium alkyl naphthalene sulfonate, 6 to 15 parts of sodium dodecyl sulfate, 110 to 160 parts of anhydrous dipotassium phosphate, 160 to 200 parts of potassium pyrophosphate, 20 to 40 parts of ethylene glycol and 6 to 15 parts of zinc sodium ethylene diamine tetraacetate. The amino acid mixture comprises the following components: aspartic acid, glutamic acid and arginine; the preparation method comprises the following steps: carrying out freeze thawing pretreatment on brown algae, and carrying out enzymolysis treatment by using cellulase, alginate lyase and mannase, so as to obtain the enzymolysis seaweed extract. All the components interact with one another, so that the synergistic effects of promoting wheat growth and germination and increasing the yield are achieved.

The invention belongs to the technical field of biology, and discloses incision type alginate lyase RiAlyPL6, an expression strain of the incision type alginate lyase RiAlyPL6 and a preparation method of unsaturated alginate oligosaccharide. The endo-algin lyase RiAlyPL6 provided by the invention comprises an amino acid fragment with a sequence as shown in SEQ ID NO: 1 or a variant fragment with at least 75% of homology with the fragment as shown in SEQ ID NO: 1. The endo-type alginate lyase RiAlyPL6 has substrate preference to poly-G, can realize high-specificity degradation of poly-G in an endo-degradation mode, and is of great significance to preparation of alginate oligosaccharide with biological activity.

The invention relates to the technical field of biological enzyme molecular modification and marine biological resource high-value utilization, and provides novel alginate lyase for specifically degrading undaria pinnatifida, and the amino acid sequence of the novel alginate lyase is shown as SEQ ID NO.2. The invention also provides a coding gene of the alginate lyase, and a recombinant expression vector and recombinant engineering bacteria containing the gene. Meanwhile, the invention also provides application of the alginate lyase in degradation of undaria pinnatifida or preparation of alginate oligosaccharide. The alginate lyase N312E is obtained through site-specific mutagenesis, and the specific enzyme activity of the alginate lyase N312E is improved by 200% compared with that of a wild type; the enzyme can specifically degrade undaria pinnatifida, the conversion rate reaches 51.5% and is increased by 58.5% compared with a wild type, the enzyme has no effect on other brown algae, and an efficient enzyme preparation is provided for high-value utilization of undaria pinnatifida and preparation of alginate oligosaccharide.

The invention provides an alginate lyase mutant. The amino acid sequence of the alginate lyase mutant is as shown in SEQ ID NO: 1 or SEQ ID NO: 3. Compared with wild-type alginate lyase, the alginate lyase mutant disclosed by the invention can be efficiently expressed, the optimal catalysis condition of the alginate lyase mutant is not changed, but the fermentation enzyme activity in the process of synthesizing alginate oligosaccharide by catalyzing sodium alginate by enzyme can be improved by 63.3% at most, so that the alginate lyase mutant is more suitable for industrial production of synthesizing alginate oligosaccharide by an enzyme method.

The invention relates to the field of genetic engineering and biological enzyme engineering, in particular to an alginate lyase mutant and application thereof in preparation of alginate oligosaccharide. According to the invention, wild-type alginate lyase is subjected to random mutation and screening to obtain a mutant which is obtained by replacing the 91st glutamic acid of the wild-type alginate lyase with aspartic acid, replacing the 256th glycine of the wild-type alginate lyase with alanine, replacing the 410th phenylalanine of the wild-type alginate lyase with proline, replacing the 531st alanine of the wild-type alginate lyase with serine, and replacing the 531st alanine of the wild-type alginate lyase with amino acid. The 568th cysteine is replaced by serine, and the amino acid sequence of the alginate lyase mutant is as shown in SEQ ID NO.3. The alginate oligosaccharide is prepared by using an alginate lyase mutant enzyme method, the problem that the polymerization degree of an existing enzymolysis product is excessively concentrated in DP2-DP3 is effectively solved, the polymerization degree distribution is widened, high-proportion enrichment of DP4-DP6 is realized, the proportion of DP4-DP6 is greater than 75%, a foundation is laid for application scene widening and high-value system application of the alginate oligosaccharide, and the alginate oligosaccharide mutant has wide application prospects. The application value of the product is obviously improved.

The invention belongs to the technical field of bioengineering, and discloses exo-alginate lyase as well as a preparation method and application thereof. The exo-algin lyase provided by the invention comprises an amino acid fragment with a sequence as shown in SEQ ID NO: 1 or a variant fragment with at least 75% homology with the amino acid fragment as shown in SEQ ID NO: 1. The exo-alginate lyase has high enzyme activity, high enzyme activity stability, high substrate specificity and high degradation efficiency, and has a good application prospect in preparation of micromolecular alginate oligosaccharide with high purity and a specific structure.

The invention discloses a sargassum fusiforme alginate lyase IDDSAly7-2 as well as a preparation method and application thereof, and belongs to the field of bioengineering, and the amino acid sequence of the alginate lyase IDDSAly7-2 is as shown in SEQ ID No.2. The degradation products of the alginate lyase IDSAly7-2 mainly comprise unsaturated disaccharide and unsaturated trisaccharide, the degradation is thorough, and the uniformity of the products is good. Natural brown algae sargassum fusiforme can be specifically degraded, the degradation efficiency is remarkably superior to that of commercial cellulase, and oligosaccharide obtained through degradation has remarkable antioxidant activity. The method is suitable for large-scale preparation of alginate oligosaccharide in a low-temperature, alkalescent and low-salt system, and a new enzymology choice is provided for high-value utilization of sargassum fusiforme resources.

The present application relates to a kind of composition for treating thyroid nodule and its preparation method, belong to biological medicine technical field, composition includes sea cucumber body wall fibrinolysis small peptide, laminaria small molecule active component, summer grass- cloud mume compound extract and safflower- chuanxiong compound extract;Sea cucumber body wall fibrinolysis small peptide is the product obtained by alkaline pectinase, bromelain and trypsin common enzymolysis after sea cucumber body wall powder, then by bacillus subtilis protease and earthworm kinase common enzymolysis. Laminaria small molecule active component is the product obtained by sea alginic acid lyase enzyme extraction, X-5 macroporous adsorption resin column purification, ultrafiltration after laminaria powder is enzymolysis. Summer grass- cloud mume compound extract is the product obtained by polyamide resin column purification, ultrafiltration after summer grass powder and cloud mume powder are extracted by ethanol aqueous solution;Safflower- chuanxiong compound extract is the product obtained by NKA-9 macroporous resin column purification, ultrafiltration after safflower powder and chuanxiong powder are extracted by ethanol aqueous solution.

The present application relates to a kind of persicobacter sp. JZB09 is preserved in China typical culture preservation center, address: Wuhan University, Wuhan City, Wuchang District, Bayi Road 299, Wuhan, Hubei Province, preservation date April 12, 2021, preservation number CCTCC M 2021354;And provide a kind of alginate lyase rAly06925 derived from persicobacter sp. JZB09, its amino acid sequence is as shown in SEQ ID NO.2, the coding gene aly06925 of the alginate lyase rAly06925, its nucleotide sequence is as shown in SEQ ID NO.1, the alginate lyase rAly06925 can degrade alginate or alginate oligosaccharide production non-reducing end contains ΔM series G-rich unsaturated oligosaccharide fragment.

The invention belongs to the technical field of biology, and discloses a preparation method and application of alginate lyase. The algin lyase is obtained by transferring a recombinant vector containing an algin lyase coding gene into a host cell for culture, expression and purification. The enzyme has relatively high catalytic activity and good substrate adaptability, and can be used for directionally preparing alginate oligosaccharide products with clear structures. The alginate lyase is suitable for efficient conversion of alginate resources, and has wide application prospects in the fields of functional oligosaccharide preparation, biological medicine, biological materials and the like.

This invention proposes an alginate lyase, a compound enzyme preparation, and their applications, belonging to the field of enzyme engineering technology. It solves the technical problem that a single alginate lyase cannot efficiently prepare alginate oligosaccharides directly from brown algae. The alginate lyase of this invention is isolated from Shewanella. Shewanella sp. HD5 The original alginate lyase gene of *Shewanella* was obtained through heterologous expression. Shewanella sp. HD5 The alginate lyase of this invention was deposited on September 22, 2025, at the China Center for Type Culture Collection, Wuhan, Hubei Province, with accession number CCTCC NO: M20252078. This invention provides a high-yield lyase obtained through heterologous expression. It is combined with cellulase, pectinase, and papain to form a complex enzyme preparation that can be directly applied to the enzymatic hydrolysis of brown algae to obtain uniformly polymerized alginate oligosaccharides. Furthermore, the complex enzyme preparation exhibits higher extraction efficiency of alginate oligosaccharides than a single alginate lyase. The prepared alginate oligosaccharides can be used in food, feed, and pharmaceutical fields.

This invention relates to a strain of *Bacillus belye* H501 and its application in promoting the growth of kelp seedlings, belonging to the field of microbiology. The strain was deposited on December 24, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC NO. 37160. The strain can grow normally within a salinity range of 15-25℃ and 1-7%, highly matching the kelp seedling cultivation environment, and does not produce alginate lyase, thus not disrupting the natural defense barrier of kelp. Applying this strain to kelp seedling cultivation can shorten the seedling cycle by approximately 3-5 days, effectively reducing energy consumption and labor costs. Furthermore, H501 exhibits significant antagonistic activity against the common aquatic pathogen *Aeromonas hydrophila*, demonstrating potential for biocontrol applications.