Esophageal tissue constructed by tissue engineering

A tissue engineering, esophagus technology, applied in the field of biomedical engineering, can solve problems such as slow progress

Inactive Publication Date: 2012-05-30
DALIAN CHUANGDA TECH TRADE MARKET
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The development of artificial esophagus is progressing slowly d...

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0012] 1) Isolation and culture of epidermal keratinocytes

[0013] The epidermis of the patient was trimmed into strips with a width of 1-2mm, rinsed twice in PBS without calcium and magnesium, and immersed in 0.5% neutral protease overnight at 4°C with the epidermis facing upward. After the epidermis was torn off from the dermis, it was cut into pieces and digested with 0.05% trypsin-0.53mM EDTA at 37°C for 20 minutes, and 10ml of trypsin inhibitor was used to stop the digestion. Filter through a 150-mesh stainless steel filter, centrifuge (1200 rpm, 10 minutes), and count to make a single-cell suspension, press 3×10 6 / 75cm 2 Density seeding culture, serum-free epidermal cell medium (GIBRO, USA), plus bovine pituitary extract and recombinant epidermal growth factor, at 37 ° C, 5 % CO 2 , cultured in an incubator with saturated humidity, and the culture medium was changed every 3 days. When the cells reached 60-75% confluence, they were digested with 0.05% trypsin at 37°C...

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Abstract

The invention belongs to the field of biomedical engineering and specifically relates to an esophageal tissue constructed by tissue engineering. A tissue-engineered esophagus containing an epithelial layer and a dermal layer is consisted of scaffold materials and seed cells according to the basic principles and methods of tissue engineering. Vascular endothelial cells in the dermal layer directly participate in the establishment of a capillary network, and two revascularization methods are used to quickly communicate the capillary network in the dermal layer with the blood supply channel of a receptor, thus providing blood to the epithelium of the tissue-engineered esophagus. The esophageal tissue of the invention changes a former therapeutic mode of repairing damage by damage, and can be used for simplifying esophagectomy and one-stage reconstruction of digestive tracts, thus reducing medical trauma and benefiting the majority of patients with esophageal diseases.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to an esophageal tissue constructed through tissue engineering. Background technique [0002] my country is a country with a high incidence of esophageal cancer and the highest mortality rate of esophageal cancer. The current clinical treatment principles are generally transthoracic resection of esophageal cancer and one-stage reconstruction of the digestive tract. The most common esophagus substitutes are the stomach, colon, and small intestine. The operation is traumatic, involves a wide range, and has many complications. The incidence of anastomotic sputum is about 3%-5%, which is a typical treatment mode of repairing trauma by trauma. The development of artificial esophagus is progressing slowly due to the absence of synthetic materials with good histocompatibility. [0003] Tissue engineering is a hot spot in life science in the 21st century. Its core conte...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38
Inventor 杜冲
Owner DALIAN CHUANGDA TECH TRADE MARKET
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