Electrofluid spraying device and method for printing three-dimensional biological scaffold

A three-dimensional biological and spray device technology, applied in coating devices, processing drive devices, manufacturing auxiliary devices, etc., can solve the problem that biological scaffolds are unfavorable for the secretion and formation of extracellular matrix, can not meet the needs of cell adhesion and growth, and are difficult to fiber. Constitute the fiber arrangement direction and other issues to achieve the effect of favorable secretion and formation, good biocompatibility, and good orientation

Inactive Publication Date: 2018-02-23
NAT UNIV OF SINGAPORE SUZHOU RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the fibers ejected by electrospinning technology are difficult to form a scaffold with controllable fiber arrangement direction, and they have disadvantages such as low porosity, poor mechanical properties, low fiber fineness, and monotonous structure.
These shortcomings lead to the formation of biological scaffolds that are not conducive to the uniform distribution of cells and the secretion and formation of extracellular matrix, nor can they meet the needs of adhesion and growth of cells of different sizes.

Method used

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  • Electrofluid spraying device and method for printing three-dimensional biological scaffold
  • Electrofluid spraying device and method for printing three-dimensional biological scaffold
  • Electrofluid spraying device and method for printing three-dimensional biological scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] The above-mentioned electrofluid jetting device is used to print a three-dimensional biological scaffold with a sine wave micro-topography and a multi-level micron-scale structure.

[0074] i) Mix polycaprolactone PCL particles and glacial acetic acid evenly, prepare a PCL solution with a concentration of 70% wt, and add the PCL solution to the syringe;

[0075] ii) Set the distance between the flat spray needle and the polished silicon crystal substrate to D=3.5mm, set the injection rate of the solution to R=1μl / min through the syringe pump, and set the movement speed of the motion platform on the X and Y axes respectively 60mm / s;

[0076] iii) The voltage between the blunt needle and the polished silicon crystal substrate is set to V=2.55kV.

[0077] The pattern of the three-dimensional scaffold printed through the above process is as follows: image 3 As shown, the pattern is characterized by the sinusoidal microtopography of its fibers.

Embodiment 2

[0079] The above-mentioned electrofluid jetting device is used to print a three-dimensional bio-scaffold with a phase-separated circle microtopography and a multi-level micron-scale structure.

[0080] i) Mix polycaprolactone PCL particles and glacial acetic acid evenly, prepare a PCL solution with a concentration of 70% wt, and add the PCL solution to the syringe;

[0081] ii) Set the distance between the flat spray needle and the polished silicon crystal substrate to D=3.5mm, set the injection rate of the solution to R=1μl / min through the syringe pump, and set the movement speed of the motion platform on the X and Y axes respectively 100mm / s;

[0082] iii) The voltage between the blunt needle and the polished silicon crystal substrate is set to V=2.55kV.

[0083] The pattern of the three-dimensional scaffold printed through the above process is as follows: Figure 4 As shown, the pattern is characterized by its fibers having phase-separated circle microtopography.

Embodiment 3

[0085] The above-mentioned electrofluid jetting device is used to print a three-dimensional bio-scaffold with adjacent circle microtopography and multi-level micro-scale structure.

[0086] i) Mix polycaprolactone PCL particles and glacial acetic acid evenly, prepare a PCL solution with a concentration of 70% wt, and add the PCL solution to the syringe;

[0087] ii) Set the distance between the flat spray needle and the polished silicon crystal substrate to D=3.5mm, set the injection rate of the solution to R=1μl / min through the syringe pump, and set the movement speed of the motion platform on the X and Y axes respectively 80mm / s;

[0088] iii) The voltage between the blunt needle and the polished silicon crystal substrate is set to V=2.55kV.

[0089] The pattern of the three-dimensional scaffold printed through the above process is as follows: Figure 5 As shown, the pattern is characterized by the microtopography of its fibers with closely adjacent circles.

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Abstract

The invention discloses a device and method for printing a three-dimensional biological scaffold. The printing method comprises the following steps that an polycaprolactone (PCL) solution after beingmixed with glacial acetic acid evenly is added into a solution supplying system; the motion speed and path of a motion platform during printing are set through a motion control system, the distance between a spraying needle and a polished silicon crystal substrate is adjusted, and the solution injection rate is set; and a power supply is adjusted, and voltage is exerted between the spraying needleand a loading platform. The three-dimensional biological scaffold which is printed by the method and has various helical structure micro morphologies has higher strength, greater contact area and higher porosity, and thus is more favorable for cell adsorption during cell seeding and differentiation and multiplication during cell culture.

Description

technical field [0001] The invention belongs to the field of electrospinning micro-nano fiber engineering, and in particular relates to an electrofluid jetting device for printing a three-dimensional biological support with micro-topography and a method for printing a three-dimensional biological support. Background technique [0002] Tissue engineering is the application of principles of life science and engineering to develop functional substitutes to reconstruct, repair or enhance tissue function. Tissue engineering has developed rapidly in the past two decades, especially in the last decade, and has the potential to repair and reconstruct many human tissues and organs, such as: skin, bone and cartilage, liver, heart valves and blood vessels, bladder, pancreas, nerves, cornea and other soft tissues, etc. Tissue engineering consists of three elements: seed cells, tissue engineering scaffolds, and tissue construction (or growth factors). Among them, porous scaffolds play ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B29C64/112B29C64/209B29C64/227B29C64/245B29C64/20B29C64/393B33Y30/00B33Y50/02A61L27/18A61L27/56
CPCA61L27/18A61L27/56B33Y30/00
Inventor 孙捷舒振刘航王丹丹傅盈西吴洋
Owner NAT UNIV OF SINGAPORE SUZHOU RES INST
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