201 results about "Hemorrhagic diseases" patented technology

The invention provides a hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit. The hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit comprises an upper shell, a lower shell, an immunostrip, test paper and the like; a gold-labeled composite containing a hemoglobin monoclonal antibody, a hemoglobin-haptoglobin composite monoclonal antibody and a transferrin monoclonal antibody is scribed on a nitrocellulose membrane; 3 test lines, a quality control line (15) and a gold-labeled composite membrane scribing line (8) are arranged on the nitrocellulose membrane in parallel. The invention further provides a preparation method and a detection method of the hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit. The hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit is convenient and simple in operation, stable in performance and accurate in result, has a relatively good reference value for early diagnosis and identification of colorectal cancer or colon cancer tumor or other tumors with lower gastrointestinal bleeding symptoms in clinic, significantly improves the positive detection rate of digestive hemorrhagic diseases, is suitable for clinical hospital examination and household self-examination, and provides measures for large-scale general examination of such diseases.

The invention relates to a composite feed additive for preventing and curing liver and gallbladder syndromes of fishes, which is mainly prepared by mixing raw materials of 50% choline chloride, green tea powder, insect chitin, selenium protein and oxidation resistant vitamins. The additive of the invention not only can prevent and cure liver and gallbladder syndromes of main fishes but also can prevent and cure hemorrhagic diseases of partial aquatic animals such as shrimps, can improve the immunity of various kinds of fishes and improve the production performance thereof, has the advantages of wide sources and easy market purchase of the adopted raw materials, simple preparation processes, convenient use and environmental protection, improves the water quality, and can not cause any harmful residues.

The invention relates to a composite developing thermosensitive gel embolizing agent as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, preparing a mixed aqueous solution of an anticancer active substance, a thermosensitive material and a developing agent into composite developing thermosensitive gel; secondly, forming a composite developing thermosensitive gel embolizing agent by the composite developing thermosensitive gel and a coagulant on the scene, wherein the thermosensitive material is hydroxyl C1-4 alkyl cellulose, Pluronic, alginate or a mixture of the substances; the anticancer active substance is arsenic trioxide, docetaxel, cisplatin, carboplatin, nedaplatin, oxaliplatin, lobaplatin, miriplatin, siRNA or a mixture of the substances; the developing agent is a water-soluble developing agent of iodixanol, ioversol or iohexol and the like. The preparation method disclosed by the invention is simple and convenient, is suitable for industrial large-scale production, is particularly suitable for preparing an embolizing agent which is biodegradable and good in biocompatibility and is used for hemorrhagic diseases, and is especially suitable for preparing the composite developing thermosensitive gel embolizing agent for treating liver cancer, kidney cancer, lung cancer, prostate cancer, uterine myoma or splenic tumor and the like.

The invention discloses a rabbit triple inactivated vaccine, a preparation method and the application thereof; the rabbit triple inactivated vaccine is formed mainly by rabbit hemorrhagic disease virus liquid which is inactivated and detoxified, rabbit pasteurella multocida liquid and rapid A type clostridium perfringens liquid which have 1:1:2 volume ratio; in the invention, by optimizing culture technology, concentration technology and immunizing dose and other measures, the rabbit triple inactivated vaccine which has good immunity effect and safety is obtained and can effectively control the rabbit hemorrhagic disease, the pasteurella multocida disease and the pasteurella multocida disease (A type) and reduce vaccine injection times of farmers, so as to reduce the stress reaction of animals and improve the production performance. The clinical application proves that the immunization effect of the rabbit triple inactivated vaccine is ideal, each rabbit is vaccinated with 2ml and has immunity after 5-7 days, the immune period is at least 180 days, and the immune safety is good without toxic and side effect.

The invention provides rabbit hemorrhagic disease virus-like particles. The rabbit hemorrhagic disease virus-like particles are prepared by optimizing rabbit hemorrhagic disease virus VP60 gene according to insect cell biased codons, connecting the optimized VP60 gene with an insect cell efficient expression vector, and then producing the rabbit hemorrhagic disease virus-like particles through a rhabdovirus/ insect cell expression system. The rabbit hemorrhagic disease virus-like particles can be mixed with a preservative and adjuvants to prepare a rabbit hemorrhagic disease vaccine. The rabbit hemorrhagic disease virus-like particles can greatly increase the expression quantity of the rabbit hemorrhagic disease virus-like particles, and have the advantages of high safety, high immunogenicity, low cost and the like, and is easy to ferment.

The invention relates to an anti-rabbit hemorrhagic disease virus (RHDV)VP60 albumen monoclonal antibody, and belongs to the technical field of biology. An SP2/0 myeloma cell and a BALB/c mouse splenic cell immunized by utilizing RHDV to recombine VP60 albumen undergo cell fusion, are selectively cultured by an HAT culture medium and undergo double ELISA screening by utilizing the recombined VP60 albumen and RHDV; the obtained cell culture supernatant is checked up and screened respectively to obtain a hybrid tumor cell strain A3C which can stably excrete the anti-RHDV VP60 albumen monoclonal antibody; the ascitic fluid ELISA titer of the A3C is detected to be 1:327,600; and according to identification, the monoclonal antibody can specifically combine the expressed recombined VP60 albumen as well as the RHDV, and one single reaction strip appears in both specific combinations, thereby proving that the anti-RHDV VP60 albumen monoclonal antibody is a VP60 specific antibody of the RHDV capsid albumen.

One kind blood samples detection method using magnetic beads, completed with the following steps: (1) Get fresh clinical samples; (2)Put these specimen on coagulation analyzer, use magnetic beads and special adjustment reagents aiming at the whole blood specimens, measure four coagulations: Activated partial thromboplastin time (APTT), Prothrombin time (PT), Prothrombin time (TT), Fibrinogen (FIB). The advantages of this invention are: 1.The method is simple, time-saving and easy to technical staff. 2. Reduce costs and save centrifuges and other equipment, reduce errors; 3. It is efficient and suitable for the battlefield, natural disasters and remote mountainous areas, medical teams. Particularly, it's suitable for hemorrhagic diseases and natural disasters (such as hemophilia, etc.). 4. Save specimen volume. 5. Reflect the level of specimens of blood coagulation preciously and accurately.

The invention relates to recombinant baculoviral of capsid protein of rabbit viral hemorrhagic disease viruses and gene engineering vaccines, which belong to the bio-pharmaceutical field. A capsid protein VP60 whole gene of the rabbit viral hemorrhagic disease viruses is amplified through RT-PCR, a baculoviral recombinant vector is constructed through the molecular biotechnology, Sf9 cells are transfected, which enables the VP60 gene to obtain expression, and then gene engineering vaccines of the rabbit viral hemorrhagic disease are prepared through taking the capsid protein VP60 of the rabbit viral hemorrhagic disease viruses of the recombinant adenovirus expression as antigen and adding corresponding adjuvant. The invention can provide the capsid protein gene engineering vaccines of the rabbit viral hemorrhagic disease with good immune effects and simple technology, which is used for preventing and controlling the rabbit viral hemorrhagic disease.

The invention discloses a construction method of a tilapia brain cell line. The method comprises the following steps: disinfecting fresh and live tilapia, taking brain tissue, cutting the tilapia intominced blocks, treating the minced blocks with collagenase, and adding trypsin; performing full suspension with a culture solution, and performing primary culture; when the bottom of a bottle is fullof cells, performing digestion with pancreatin-EDTA, and performing inoculation for secondary culture; and after 60 generations of secondary culture, adding colchicine in the logarithmic growth phasefor treatment, and performing karyotype analysis. The preparation method of the tilapia brain cell line, disclosed by the invention, is simple and convenient; and the tilapia brain cell line preparedby the method can perform secondary culture for a long time, is very sensitive to hemorrhagic disease viruses of tilapia, and can be widely applied to experiments.

The invention relates to a colloid gold duplex test strip for detecting fecal occult blood and a preparation method thereof. The colloid gold duplex test strip is composed of a bottom lining, a sample pad, a marker pad, a coating film and bibulous paper, wherein the sample pad, the marker pad, the coating film and the bibulous paper are sequentially adhered to the bottom lining in a lap joint mode. The marker pad comprises a colloid gold marked mouse against human Hb monoclonal antibody Hb1 and a mouse against human Tf monoclonal antibody Tf1, the concentration of the mouse against human Hb monoclonal antibody Hb1 is 5-20 Mu g/mL, and the concentration of the mouse against human Tf monoclonal antibody Tf1 is 5-20 Mu g/mL. According to the colloid gold duplex test strip, hemoglobin and transferrin in faeces are detected synchronously, the specificity is high, the sensitivity is high (the detection limit of Hb is 200 ng/ml, and the detection limit of Tf is 40 ng/ml respectively), the positive detection rate of the digestive tract hemorrhagic disease is improved remarkably, the asymptomatic, slight and continuous alimentary tract hemorrhage which cannot be seen by naked eyes and a microscope can be accurately detected, and the colloid gold duplex test strip can avoid the interference of medicine, such as animal blood and so on.

The invention relates to a rabbit hemorrhagic disease virus baculovirus vector and pasteurella multocida bivalent inactivated vaccine and a preparation method thereof, and belongs to the field of immune technology. Recombinant rabbit hemorrhagic disease virus VP60 baculovirus is inoculated into Sf9 insect cells and cultured at 27-28 DEG C. When cell lesion reaches 85% or more, a cell culture is harvested and inactivated, and the inactivated cell culture is used as a rabbit hemorrhagic disease virus antigen. Rabbit Pasteurella multocida capsular serotype A C51-17 strain is amplified and cultured, a bacterial solution is inactivated, and the inactivated bacterial solution is used as a Pasteurella multocida antigen. The rabbit hemorrhagic disease virus baculovirus vector and pasteurella multocida bivalent inactivated vaccine can be prepared by mixing the rabbit hemorrhagic disease virus antigen and the Pasteurella multocida antigen with adjuvants in proportion. The rabbit hemorrhagic disease virus baculovirus vector and pasteurella multocida bivalent inactivated vaccine has high safety, good immune effect and simple process, and can be used for preventing and controlling Rabbit Hemorrhagic Disease (Rabbit Plague) and Rabbit Pasteurella multocida.

The invention provides a method for preparing an inactivated bivalent vaccine against a rabbit hemorrhagic disease and a pasteurella multocida disease, which comprises the following steps: inoculating a rabbit hemorrhagic disease strain CD85-2 strain to a rabbit, aseptically collecting the heart, liver, spleen, lung or kidney of the rabbit dead in 24 to 96h, and removing the connective tissues on the heart, liver, spleen, lung or kidney; preparing the mashed and filtered heart, liver, spleen, lung or kidney into an emulsion after mashing and filtering, and inactivating the emulsion; reactivating a rabbit originated capsula group A pasteurella multocida strain C51-17 strain, performing propagation culture on the reactivated strain, and inactivating the liquid C51-17 strain containing the A type pasteurella multocida strain obtained by culture; and mixing the inactivated liquid strain and an adjuvant, concentrating the mixture to obtain the liquid strain containing the A type pasteurella multocida C51-17, mixing the liquid strain and the inactivated emulsion to obtain the inactivated bivalent vaccine. The inactivated bivalent vaccine prepared by the method has the advantages of high safety, high vaccination efficacy and long shelf life.

The invention relates to a rabbit hemorrhagic disease virus(RHDV) colloidal gold test strip, and belongs to the technical field of immune tests of the colloidal gold. One end of a bottom plate of the test strip is a colloidal gold-RHDV polyclonal antibody combination glass fiber film, and an antibody solid-phase cellulose nitrate film is arranged on the middle part of the bottom plate; a RHDV single-resistance test line, namely T line and a goat anti-rabbit IgG control line, namely C line are arranged on the bottom plate; and the other end of the bottom plate is absorbent paper. When a tested sample is carried with the RHDV, the RHDV can specifically combine the anti-RHDV polyclonal antibody labeled by the colloid so as to be identified by the anti-RHDV monoclonal antibody, namely the double-antibody sandwich specific combination happens, so that whether the sample is carried with the RHDV can be tested. The invention also provides a RHDV immunological test method established by utilizing the monoclonal antibody and the polyclonal antibody, which has the advantages of quickness, accuracy, convenience and the like, and the method is particularly suitable for the on-the-spot test and preliminary screening test and has bright prospect.

An application of the plants in Ypsilandra family in preparing the medicines for treating the hemorrhagic diseases (menorr hagia, postpartum hemorrhage, metrorrhagia, etc) is disclosed. Said plants are chosen from the Ypsilandra thibetica. Y.yunnanensis, Y.alpina, Y cavalevici, and Y kansuensis. Its advantages are high curative effect, low cost and high safety.

The invention provides a primer and a TaqMan probe for fluorescence quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection of a rabbit hemorrhagic disease virus. The invention also provides a kit comprising the primer and the probe, and a method for detecting the rabbit hemorrhagic disease virus by using the primer and the probe with one-step RT-PCR. Due to the adoption of a one-step RT-PCR detection technology, reverse transcription and quantitative PCR amplification are continuously completed in one tube, amplification and detection are performed in a totally-closedtube, the probability of pollution caused by multiple operation is reduced, operation is easy, the volume loss in the sampling process is reduced, the dependability of a result is ensured, all cDNA (complementary Deoxyribonucleic Acid) samples obtained by reverse transcription are used for amplifying, and higher sensitivity is achieved.

The invention relates to C27 spirostane-type steroidal saponins isolated from Ypsilandra, Trillium and Paris and their preparation method, as well as their application in the preparation of drugs for treating hemorrhagic diseases, and application in the preparation of drugs for treating dysfunctional uterine bleeding, and the preparation of functional daily chemical products and health products. The materials used in a method of the invention are easy to obtain, the method is simple and easily operable, biological experiments of the compound produced show that the compound can induce platelet aggregation activity and hemostatic activity significantly.

The invention relates to a method for preparing liquid fibrinogen (FIB) detection solution. The solution is mainly used for monitoring the disturbance of blood coagulation, the hemorrhagic disease, the disseminated intravascular coagulation (DIC), the diabetes, the acidosis, the atherosclerosis and other disease symptoms. Along with the wide clinical application of thrombus and hemostasis detection, the fibrinogen (FIB) detection is the routine detection item of the thrombus and hemostasis detection, and has important clinical diagnosis value. The method adopts cow thrombin as a main raw material, and uses the Von clauss method (NCCLS recommended) to prepare the novel liquid fibrinogen (FIB) detection solution. The novel stable system solves the difficulty of maintaining the stability of the solution in the liquid state, and the method has the advantages of high sensitivity, convenient use, low experimental error, high stability, favorable compatibility, low cost, and the like.

A freeze dried tranexamine acid powder used as antihemorrhagic injection for treating various hemorrhagic diseases contains tranexamine acid (0.05-10 wt. portions), freeze drying supporting agent (0-100) and pH regulator.

The invention discloses a traditional Chinese medicine containing pseudo-ginseng powder and a preparation method thereof. The traditional Chinese medicine comprises, by mass, 60-100 parts of pseudo-ginseng powder, 20-30 parts of red sage root, 10-20 parts of licorice, 30-40 parts of radix rehmanniae, 15-25 parts of root of kudzu vine, 40-50 parts of radix astragali, 20-30 parts of bighead atractylodes rhizome, 60-80 parts of dextrin and 5-7 parts of powdered steatile. The traditional Chinese medicine utilizes pseudo-ginseng powder, red sage root and licorice as raw materials, does not contain hormones and antibiotics in Western medicines, and does not produce drug resistance. Natural plants adopted by the preparation method has no obvious toxic or side effects in research, have high safety, can be absorbed easily, can produce effects fast and are convenient for use. The traditional Chinese medicine can treat inner and outer hemorrhagic diseases such as a bleeding wound, blood stasis, gastrorrhagia and hematuria, has effects of expanding blood vessel, dissolving thrombus, improving microcirculation, preventing and treating cardiovascular and cerebrovascular diseases such as hyperlipidemia, cholesterol increasing, coronary heart disease, stenocardia and cerebral hemorrhage sequel, treating liver diseases such as fatty liver and liver fibrosis, and treating physical deficiency caused by hemorrhage, delivery and aeipathia, has a low cost and simple processes, and can be widely used.

The invention relates to a rabbit hemorrhagic disease virus recombinant subunit vaccine and a preparation method thereof. A vaccine production strain is a recombinant autographa californica nuclear polyhedrosis virus CGMCC No.8052 (China General Microbiological Culture Collection Center Number 8052), an expression vector adopted by the strain is a secretory expression vector and is provided with six histidine tags easy for protein detection and analysis; the strain is used as the production strain for producing the rabbit hemorrhagic disease virus recombinant subunit vaccine; the vaccine is free from viral genome, animal safety hazard and potential dispersity, is high in immunity pertinence, and can be used for exciting immunized animals to generate sufficient immune antibodies and long protective efficacy. RHDV-like (rabbit hemorrhagic disease virus-like) particles are produced according to the technology provided by the invention, the hemagglutination titer of virus liquid obtained from a 1000L bioreactor is (1 to 32768) to (1 to 262144), the protein expression quantity of the RHDV particles is about 150mg/L, 1L of semi-finished virus liquid can be prepared into 5,000-100,000 standard vaccines, and the preparation efficiency is higher than the domestic technical level by 8-20 times; the recombinant subunit vaccine is added with an adjuvant, so that the immune effect of the vaccine is improved; and the vaccine is a novel vaccine remarkably superior to an inactivated tissue vaccine produced in the prior art.

The invention relates to the field of animal medicine, in particular to a method for detecting duck plague virus (DPV) antibodies. The method specifically comprises the steps of preparation of solid phase antigens, primary antibody combination, secondary antibody combination, color development, detection, judgment and the like. The method is a purified recombinant UL55 protein-based indirect enzyme-linked immuno sorbent assay (ELISA) method, and has good specificity; the results of detecting the positive serums of duck virus hepatitis viruses (DHV), riemerella anatipestifer (RA), duck escherichia coli (E.coli), duck-derived salmonella, duck swollen head hemorrhagic disease viruses and duck-derived influenza viruses are negative; and the repeated tests in enzyme-labeled plates or between enzyme-labeled plates display that variation coefficients are less than 10 percent, and the method can detect out the positive serums of DPV weak virus vaccine immune ducks, wherein the DPV weak virus vaccine are diluted according to a ratio of 1:6400.

The invention discloses specific amplification primers for detecting trionyx sinensis hemorrhagic disease viruses and a detection kit using the same. The amplification primers solve the problem that at present, viral pathogens causing trionyx sinensis explosive death cannot be accurately diagnosed. The specific amplification primers for detecting trionyx sinensis hemorrhagic disease viruses can produce a specific detection result, can be determined easily and is conducive to trionyx sinensis virus disease definite diagnosis. The invention also provides a detection kit for detecting trionyx sinensis hemorrhagic disease viruses. The detection kit contains the specific amplification primers. The detection kit is a common RT-PCR kit or a fluorescent quantitative RT-PCR kit. The two types of kits have high specificity and high sensitivity, can be operated simply, and fill a domestic gap in a trionyx sinensis hemorrhagic disease virus nucleic acid molecule detection method, and is suitable for definite diagnosis, screening and prevention of trionyx sinensis hemorrhagic disease viruses.

The invention relates to a traditional Chinese medicine combination for treating gynaecological hemorrhagic disease, belongs to the traditional Chinese medicine field. The invention is characterized in that the traditional Chinese medicine combination is made from raw ingredients by weight parts as follows: Prepared Nutgrass Galingale Rhizome is 8 to 25 parts, typha orientalis stir-fried with vinegar is 5 to 20 parts, Myrrha Preparata is 8 to 20 parts, panpax notoginseng is 3 to 15 parts, stir-frying bitter orange is 8 to 20 parts, leonurus japonicus is 8 to 25 parts, and agrimony is 8 to 25 parts. The invention has the efficacies of thoroughfare regulating as well as hemostasia, and blood stasis and detoxication dissolving. The traditional Chinese medicine combination is mainly used for dysfunctional uterine bleeding, blood menostaxis, excess menstrual blood, postpartum lochiorrhea, the uterine bleeding caused by curettage, intrauterine device and induced labor, hysteromyoma, and vaginal bleeding caused by cervical erosion. The traditional Chinese medicine combination can achieve hemostasia purpose on various syndromes of deficiency complicated with excess.

The invention belongs to the technical field of biology and particularly relates to an oral vaccine for hemorrhagic disease of grass carp as well as preparation and application of the oral vaccine. The invention firstly provides fusion protein containing bacillus subtilis spore capsid protein CotC and grass carp reovirus VP4 protein, wherein the amino acid sequence of the grass carp reovirus VP4 protein corresponds to the amino acid sequence encoded by the nucleotide sequence shown as SEQ ID NO.8. The oral vaccine prepared by the fusion protein has the advantages of simple preparation process,low cost, convenience in oral administration and the like; meanwhile, the damage of fishing and injecting to the fish bodies in the injection immunization scheme is avoided. In addition, the oral vaccine has better immune protection effect on fish body resistance to of the hemorrhagic disease of the grass carp compared with an existing vaccine.