The invention relates to a diagnostic kit for a Newcastle disease virus antibody, which comprises 10-time concentrated washing liquid, serum diluent, standard serum, an anti-chicken immunoglobulin G (IgG) enzyme-labeled antibody, enzyme-labeled antibody diluent, a color development agent A, a color development agent B and stop solution. In an enzyme-linked immunosorbent assay (ELISA) diagnostic kit for detecting the Newcastle disease virus antibody, the established method has specificity, sensitivity and operability. In the aspect of sensitivity tests, the coincidence rate of the established method and a method of immunofluorescence technology is 86 percent, and compared with the method of the immunofluorescence technology, the established method is more sensitive and has the zero relevance ratio in other causes of disease pathogeny; and compared with hemagglutination inhibition (HI), the method has the advantages of quickness and low cost, and can be used for diagnosing and monitoring Newcastle disease virus, and the result is easy to determine.