40 results about "Heparin-DHE" patented technology

The present invention relates to a chimeric protein that includes an N-terminus coupled to a C-terminus, where the N-terminus includes a portion of a paracrine fibroblast growth factor (“FGF”) and the C-terminus includes a C-terminal portion of an FGF19 molecule. The portion of the paracrine FGF is modified to decrease binding affinity for heparin and/or heparan sulfate compared to the portion without the modification. The present invention also relates to pharmaceutical compositions including chimeric proteins according to the present invention, methods for treating a subject suffering from diabetes, obesity, or metabolic syndrome, and methods of screening for compounds with enhanced binding affinity for the βKlotho-FGF receptor complex involving the use of chimeric proteins of the present invention.

The invention relates to a bioactive hydrogel as a hybrid material of heparin and star-branched polyethylene glycol with functionalized end groups, wherein the heparin is bound directly by reaction of the carboxyl groups activated with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimides/N-hydroxysulfosuccinimide (EDC/s-NHS) with the terminal amino groups of the polyethylene glycol covalently by amide bonds.

The invention discloses a method for preparing a mesoporous silicon nanoparticle coating loaded with heparin and Cu<2+> on the surface of a biological material. The method comprises the steps of carrying out surface modification on mesoporous silicon nanoparticles to obtain amino-modified mesoporous silicon nanoparticles to load heparin and Cu<2+> into a mesoporous duct; modifying the surfaces of the nanoparticles by using albumin; and finally fixing the nanoparticles on the surface of the dopamine-modified biological material to obtain the mesoporous silicon nanoparticle coating loaded with the heparin and the Cu<2+>. Surface modification is carried out on a material or device implanted into a blood vessel by adopting the method, so that the loading capacity and the release behavior of the heparin and the Cu<2+> on the surface of the material can be effectively controlled, the material is endowed with good blood compatibility and regeneration of blood vessel endothelium can be significantly promoted, thereby effectively improving the biocompatibility and the implant success rate of the material.

Compositions and methods of employing compositions in flushing and coating medical devices are disclosed. The compositions include combinations of a chelating agent, anticoagulant, or antithrombotic agent, with a C₄-C₉ carboxylate antimicrobial agent, such as octanoic acid. Methods of using these compositions for coating a medical device and for inhibiting catheter infection are also disclosed. Particular combinations of the claimed combinations include, for example, octanoic acid or other C₄-C₉ carboxylate antimicrobial agent together with EDTA, EGTA, DTPA, heparin and/or hirudin in a pharmaceutically acceptable diluent.

The invention discloses a preparation method and application of cross-linked hydrogel for muscle stem cell culture, and belongs to the technical field of biological food materials. Chitosan, alginate, dextran and Ca<2+> are crosslinked through a physical crosslinking method to form double-network hydrogel with high mechanical strength, and then the hydrogel is coated with heparin and collagen through a dipping coating method, so that the hydrogel can immobilize growth factors and adhere to cells. Meanwhile, the extracted primary muscle stem cells are inoculated onto the hydrogel and cultured for 24 hours in a growth culture medium (79% of DMEM, 10% of FBS and 1% of double antibodies); and then the extracted primary muscle stem cells are cultured in an incubator with a differential medium (97% of DMEM, 2% of horse serum and 1% of double antibodies) for 7 days. By utilizing the hydrogel, the absorption of the muscle stem cells on nutrient substances can be enhanced, and the growth of the muscle stem cells is facilitated. The dual-network hydrogel has the potential to be used as a scaffold for muscle stem cell growth of stem cell culture meat.

The invention relates to a local application emplastrum with analgesic activity and simultaneously capability of enabling hematoma reabsorption, which comprises a basal layer, a pharmaceutically acceptable diclofenac salt, a hydrogel matrix form adhesion layer with heparin or heparitin, and a protective membrane capable of being removed in use.

The invention provides a woven reinforced degradable polyurethane elastomer artificial blood vessel and a preparation method thereof. The inner layer of the artificial blood vessel adopts activated H2N-PEG-COOH, heparin and (Mpa)-GGGGGREDV polypeptide to treat a C-PEEUU-NH2 electrospun nanofiber tubular stent to obtain a C-PEEUU-PEG-Hep/REDV tubular stent, the middle layer adopts a woven reinforced layer of nanofiber yarns woven by dissolving and mixing one or more master batches of PLGA, PDO and PGA according to a mass ratio, and the outer layer is a macroporous layer prepared by thermally induced phase separation. The woven reinforced degradable polyurethane elastomer artificial blood vessel provided by the invention can be used for tissue regeneration and construction of cardiovascularand cerebrovascular diseases, the preparation method is simple and efficient, the price is low, and the prepared artificial blood vessel has resilience, compliance, bursting strength and biocompatibility matched with autologous vascular tissues. The artificial blood vessel has no hypersensitivity to a human body in a transplantation operation, has affinity with blood and peripheral tissues in thehuman body, and has a good application prospect in repair operations of cardiology surgery, nephrology surgery and brain surgery.

Disclosed herein are cellular derived heparin and heparan sulfate compositions, methods of making and using, and cell lines for making and using.

The invention discloses a purifying column, device and method for purifying blood. An inner cavity of a columnar container in the purifying column is divided into a first space and a second space through a semipermeable membrane; agglutinin or a mixture formed by mixing heparin and agglutinin according to a preset proportion is treated as an adsorption medium to coat a solid substrate, and then the solid substrate is transferred into dialysate; the dialysate containing the adsorption medium flows in the same direction as blood in the first space and in the second space, and therefore the adsorption medium can effectively adsorb substances to be adsorbed (such as pathogens and tumor secretory protein) in the blood through the semipermeable membrane; and meanwhile, due to the fact that the dialysate containing the adsorption medium dynamically flows in the second space, after the dialysate is used for a long time and the adsorption medium loses efficacy, a dialysis column does not need to be replaced, and medical resources are saved.

The invention relates to a urethral catheter and a preparation method thereof. The urethral catheter comprises a catheter body, a coating formed on the surface of the catheter body, and heparin and chitosan which are self-assembled on the outer surface of the coating layer by layer, wherein the coating is formed by a coating composition, and the coating composition comprises at least one photocurable polymer. According to the urethral catheter, the damage of the urethral catheter to the inner surface of the urethra can be avoided. Meanwhile, accumulation of crystals can be effectively inhibited, bacteria adhered to the surface of the urethral catheter and the wound surface of the urethra can be killed, and the risk of bacterial infection is reduced.

The present invention relates to locking compositions, kits containing the locking compositions and methods for maintaining the patency of vascular access devices or intravascular delivery devices employing the locking compositions. The locking compositions contain water, an anticoagulant and a viscosity agent and are free of heparin and alcohol.

Isolated heparin or heparan sulphate oligosaccharide fragments having a chain length of at least 10 saccharides and no more than 50 saccharides, which are capable of binding BMP2, are disclosed. Also disclosed is the use of the same heparin or heparan sulphate oligosaccharide fragments in kits and pharmaceutical compositions, and the use of the same heparan sulphate oligosaccharide fragments in the repair and/or regeneration of connective tissue and bones, and the treatment of wounds.

The present invention relates to a chimeric protein that includes an N-terminus coupled to a C-terminus, where the N-terminus includes a portion of a paracrine fibroblast growth factor (“FGF”) and the C-terminus includes a C-terminal portion of an FGF21 molecule. The portion of the paracrine FGF is modified to decrease binding affinity for heparin and/or heparan sulfate compared to the portion without the modification. The present invention also relates to pharmaceutical compositions including chimeric proteins according to the present invention, methods for treating a subject suffering from diabetes, obesity, or metabolic syndrome, and methods of screening for compounds with enhanced binding affinity for the βKlotho-FGF receptor complex involving the use of chimeric proteins of the present invention.

The invention provides a glycosaminoglycan composition and a preparation method and application thereof. The composition is extracted and prepared from a ruminant heparin byproduct glycosaminoglycan,consists of fast-moving heparin and dermatan sulfate, and has anticoagulation and antithrombosis effects, the antithrombin III-dependent anti-Xa activity is 60-100 U/mg, the heparin cofactor II-dependent anticoagulation activity is 100-200 U/mg, and the sheep plasma method anticoagulation activity is 50-80 U/mg. The composition can be developed into a medicine or a health care product, and has halal property. In addition, organs of ruminants such as sheep and cattle, and glycosaminoglycan as a by-product of heparin production, as raw materials of the composition are simple, convenient and easily available, and thereby economic effects are remarkable.