GeXP rapid detection kit capable of simultaneously identifying nine pathogens of chicken respiratory tract diseases

Abstract

The invention discloses a GeXP rapid detection kit capable of simultaneously identifying nine pathogens of chicken respiratory tract diseases. The kit is used based on a GeXP system and comprises ten polymerase chain reaction (PCR) primer pairs; the kit is used for identifying and detecting avian influenza virus, H5, H7 and H9 subtype avian influenza virus, newcastle disease virus, infectious bronchitis, infectious laryngotracheitis, mycoplasma gallisepticum, bursa synovialis mycoplasma and haemophilus paragallinarum; and the kit is good in specificity, high in sensitivity and can detect 100 copy / mu l. Compared with an identifying result of the conventional experiment method of a pathogen separation and hemagglutination inhibition experiment or a serology experiment and the like, the GeXP rapid detection kit has the advantage that the coincidence rate reaches 100 percent. The kit is generally used for detecting the main chicken respiratory tract diseases and the pathogens thereof, so that a simple and high-flux detection kit and a detection system are provided, an actual requirement is met, and the application prospect is wide.

Description

technical field

[0001] The invention relates to a GeXP rapid detection kit for simultaneously identifying nine kinds of chicken respiratory disease pathogens. Background technique

[0002] H5, H7 and H9 subtype avian influenza virus, Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, mycoplasma gallisepticum, mycoplasma synovialum and infectious rhinitis are the nine main respiratory infections that seriously harm chickens disease. The main clinical manifestations are: rales, cough, wheezing, neck stretching, mouth breathing, head shaking and dyspnea. These infectious diseases infect chickens of different ages and have high morbidity and mortality, but their clinical symptoms are very similar and cannot be identified. The traditional methods for differential diagnosis of these respiratory diseases mainly include pathogen isolation and identification and serological tests, etc., but these methods are often limited by the freshness of c...

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