30 results about "Haemophilus paragallinarum" patented technology

A novel peptide obtained from Haemophilus paragallinarum has been found useful for preventing avian infectious coryza. This polypeptide induces production of hemagglutination-inhibition antibody and prevents infection and onset of avian infectious coryza. The invention further provides a gene coding for the polypeptide, a recombinant vector for expression of this gene, a host transformed with this vector, a process for preparing the polypeptide in a host, a vaccine for avian infectious coryza comprising the polypeptide as an active ingredient, a monoclonal antibody obtained using the polypeptide as an immunogen, and a diagnostic agent and a therapeutic agent for avian infectious coryza using the peptide and the antibody.

The invention discloses a GeXP rapid detection kit capable of simultaneously identifying nine pathogens of chicken respiratory tract diseases. The kit is used based on a GeXP system and comprises ten polymerase chain reaction (PCR) primer pairs; the kit is used for identifying and detecting avian influenza virus, H5, H7 and H9 subtype avian influenza virus, newcastle disease virus, infectious bronchitis, infectious laryngotracheitis, mycoplasma gallisepticum, bursa synovialis mycoplasma and haemophilus paragallinarum; and the kit is good in specificity, high in sensitivity and can detect 100 copy / mu l. Compared with an identifying result of the conventional experiment method of a pathogen separation and hemagglutination inhibition experiment or a serology experiment and the like, the GeXP rapid detection kit has the advantage that the coincidence rate reaches 100 percent. The kit is generally used for detecting the main chicken respiratory tract diseases and the pathogens thereof, so that a simple and high-flux detection kit and a detection system are provided, an actual requirement is met, and the application prospect is wide.

The invention relates to a haemophilus parasuis detection kit and a detection method thereof, and belongs to the technical field of molecular biology. The detection kit comprises a primer pair, PCR Mix, a position control and dd H2O. The haemophilus parasuis detection kit disclosed by the invention has the primer pair designed according to an mviN gene sequence in a high conserved domain, is good in specificity, and can accurately distinguish the haemophilus parasuis strain LC from haemophilus paragallinarum, actinobacillus pleuropneumoniae, pasteurella muhocida, arcanobacterium pyogenes, staphylococcus aureus and streptococcus suis; the detection kit and the detection method provided by the invention are high in sensitivity, short in consumed time, accurate in detection, and important in significance of monitoring haemophilus parasuis reproduction, disease occurrence and prevalence as well as timely control of the disease.

The invention relates to the antigen imitated epitope peptide of Haemophilus paragallinarum serovar A in China and its application, wherein its amino acid sequence is represented by the general formula of X1X2X3X4AX5DPVX6X7X8, wherein A represents alanine residue, D represents aspartate residue, P represents proline residue, V represents valine residue, X1-X8 represents any amino acids. The invention also discloses the use of the epitope peptide of Haemophilus paragallinarum serovar A -type in China as active ingredient in the preparation of vaccine for treating chicken infectious rhinitis.

The invention discloses a preparation method of an inactivated vaccine of infectious coryza of chicken, which comprises the following steps: firstly, diluting haemophilus paragallinarum strains, inoculating a chicken agar plate, culturing at 37 DEG C for 22-24h; selecting a typical colony to inoculate a chicken agar slant culture-medium, culturing to obtain first class slant strains; secondly, getting the first class slant strains to inoculate into a chicken soup culture medium, vibrating and culturing at 37 DEG C for 12h, obtaining second class strains; thirdly, inoculating the second class strains into a semisynthetic culture medium, culturing by a biological fermentation cylinder; and inactivating to obtain the inactivated vaccine. Aiming at defects of complex process, high production cost, low yield and the like existing in the prior preparation method of the inactivated vaccine of infectious coryza of chicken, the research work of an improved production process is developed, and the important breakout on the culture method is obtained. The method has simple and feasible operation; compared with the prior art, the invention greatly improves the culture strains, shortens the culture time by 10-12h, and effectively lowers the production cost.