30 results about "Haemophilus paragallinarum" patented technology

The invention discloses a GeXP rapid detection kit capable of simultaneously identifying nine pathogens of chicken respiratory tract diseases. The kit is used based on a GeXP system and comprises ten polymerase chain reaction (PCR) primer pairs; the kit is used for identifying and detecting avian influenza virus, H5, H7 and H9 subtype avian influenza virus, newcastle disease virus, infectious bronchitis, infectious laryngotracheitis, mycoplasma gallisepticum, bursa synovialis mycoplasma and haemophilus paragallinarum; and the kit is good in specificity, high in sensitivity and can detect 100 copy/mu l. Compared with an identifying result of the conventional experiment method of a pathogen separation and hemagglutination inhibition experiment or a serology experiment and the like, the GeXP rapid detection kit has the advantage that the coincidence rate reaches 100 percent. The kit is generally used for detecting the main chicken respiratory tract diseases and the pathogens thereof, so that a simple and high-flux detection kit and a detection system are provided, an actual requirement is met, and the application prospect is wide.

The invention provides a preparation method of a chicken infectious rhinitis and mycoplasma gallisepticum bivalent lipid inactivated vaccine, belonging to the technical field of biological products for animals. The preparation method is implemented by the following steps of: preparing a type A haemophilus paragallinarum inactivated antigen bacterial liquid, a type C haemophilus paragallinarum inactivated antigen bacterial liquid and a mycoplasma gallisepticum antigen liquid; uniformly mixing the type A haemophilus paragallinarum inactivated antigen bacterial liquid with the type C haemophilus paragallinarum inactivated antigen bacterial liquid in the volume ratio of 1:1 to obtain a mixed haemophilus paragallinarum inactivated antigen bacterial liquid; uniformly mixing the mixed haemophilus paragallinarum inactivated antigen bacterial liquid with the mycoplasma gallisepticum antigen liquid in the ratio of 1:1-1:1.5, pouring into an emulsifying tank and stirring; slowly adding poplar bark lipid till the final volume percentage concentration of the poplar bark lipid in the mixture is 2.0-3.8 percent; and continually stirring and emulsifying for 30-60 minutes. The vaccine has the advantages of easiness for absorbing, no toxic or side effect, short immunogenic time, long immune duration, good immune effect and capability of effectively preventing chicken infectious rhinitis and mycoplasma gallisepticum.

The invention provides a preparation method of a chicken infectious rhinitis lipid inactivated vaccine, belonging to the technical field of biological products for animals. The preparation method is implemented by the following steps of: preparing a type A haemophilus paragallinarum inactivated antigen bacterial liquid and a type C haemophilus paragallinarum inactivated antigen bacterial liquid; uniformly mixing the type A haemophilus paragallinarum inactivated antigen bacterial liquid with the type C haemophilus paragallinarum inactivated antigen bacterial liquid in the volume ratio of 1:1, and pouring into an emulsifying tank; stirring with a mixer; slowly adding poplar bark lipid at a low speed till the final volume percentage concentration of the poplar bark lipid in the mixture is 2.0-3.8 percent; and continually stirring and emulsifying for 30-60 minutes to obtain the vaccine, wherein the final bacterium content of a type A strain and the final bacterium content of a type C strain in the vaccine are 1,000,000,000 cfu/ml. The vaccine has the advantages of easiness for absorbing, no toxic or side effect, short immunogenic time, long immune duration, good immune effect and capability of effectively preventing chicken infectious rhinitis and mycoplasma gallisepticum.

The invention relates to a haemophilus parasuis detection kit and a detection method thereof, and belongs to the technical field of molecular biology. The detection kit comprises a primer pair, PCR Mix, a position control and dd H2O. The haemophilus parasuis detection kit disclosed by the invention has the primer pair designed according to an mviN gene sequence in a high conserved domain, is good in specificity, and can accurately distinguish the haemophilus parasuis strain LC from haemophilus paragallinarum, actinobacillus pleuropneumoniae, pasteurella muhocida, arcanobacterium pyogenes, staphylococcus aureus and streptococcus suis; the detection kit and the detection method provided by the invention are high in sensitivity, short in consumed time, accurate in detection, and important in significance of monitoring haemophilus parasuis reproduction, disease occurrence and prevalence as well as timely control of the disease.

The invention discloses an indirect ELISA kit for detecting Haemophilus paragallinarum type A antibody, a detection method and an application thereof, and relates to the technical field of biological detection. The ELISA kit of the present invention includes: coated microplate, washing solution, serum diluent, substrate chromogenic solution, rabbit anti-chicken enzyme-labeled secondary antibody, stop solution, type A Hpg standard positive serum and type A Hpg standard negative serum Serum; wherein, the coated microtiter plate uses the recombinant hemagglutinin protein of Haemophilus paragallinarum type A as the coated antigen. The ELISA kit of the present invention is used to detect the antibody of Haemophilus paragallinarum type A, and has the advantages of high efficiency, good sensitivity, specificity and repeatability, and the kit is easy to operate, fast and low in cost, and is suitable for clinical applications and promote.

The invention relates to a kit and more particularly relates to a kit for PCR (Polymerase Chain Reaction) detection of haemophilus paragallinarum. The kit comprises 20 microliters of PCR buffer solution, 25 microliters of ultrapure water, 5 microliters of Marker DL2000, 3 microliters of 15pmol/microliter upper primers, 3 microliters of 15pmol/microliter lower primers, 3 microliters of polymerase, 3 microliters of bromophenol blue, 15 microliters of TE buffer solution, 5 microliters of negative control and 5 microliters of positive control. The kit has the beneficial effects that the kit has the advantage of rapid detection of a PCR detection technology and the kit is low in construction cost; the special primers are adopted so as to meet the detection of a PCR product of a gene segment which is 0.00953ng at the minimum; the detection sensitivity is high.

The invention discloses a haemophilus paragallinarum fluorogenic quantitative PCR detection method. According to the haemophilus paragallinarum fluorogenic quantitative PCR detection method, a hagA gene with the high degree of specificity and conservative property is selected as an amplification target gene, SYBR Green I fluorochrome is taken as a detection target, through the continuous optimization of fluorogenic quantitative PCR reaction conditions, the preparation of a standard substance and the establishment of a standard curve, the haemophilus paragallinarum fluorogenic quantitative PCR detection method of the SYBR Green I fluorochrome jogged with DNA, and the DNA of a haemophilus paragallinarum hagA genetic template can be quantified accurately. The haemophilus paragallinarum fluorogenic quantitative PCR detection method has the advantages of being strong in specificity, high in sensibility, simple in operation, rapid in detection, accurate in quantification and the like, the haemophilus paragallinarum fluorogenic quantitative PCR detection method can be used for clinic rapid diagnosis of haemophilus paragallinarum and the quantitative analysis of the degree of infection by the haemophilus paragallinarum, and the haemophilus paragallinarum fluorogenic quantitative PCR detection method is suitable for popularization and application.

The invention relates to an inactivated vaccine for infectious coryza of chickens. The preparation method of the inactivated vaccine for infectious coryza of chickens comprises the following steps: using sterilized PBS (phosphate-buffered saline) to dilute inactivated haemophilus paragallinarum A type C-hpg-8 strain concentrated bacteria liquid prepared by the conventional method by a certain proportion, then mixing with vaccine adjuvants taking carbomer as a main component according to the proportion, stirring and shaking up so as to prepare the inactivated vaccine for infectious coryza of chickens. After a chicken is immunized by the inactivated vaccine for infectious coryza of chickens, the following advantages can be realized: the antibody titer generated by the inoculated chicken is high, the protection period of the antibody is long, and the protection rate against strong virus is high and the like.

The invention relates to the antigen imitated epitope peptide of Haemophilus paragallinarum serovar C in China and its application, wherein its amino acid sequence is represented by the general formula of YX1PX2QWWX3X4X5X6X7, wherein Y represents tyrosine residue, P represents proline residue, Q represents glutamine residue, W represents tryptophan residue, X1-X7 represents any amino acids. The peptide can be used for providing an easily-manipulated serological type differential diagnosis method for chicken infectious rhinitis.

The invention discloses a haemophilus paragallinarum B strain fermentation medium, a preparation method thereof and application thereof, and belongs to the field of agricultural microorganism. The haemophilus paragallinarum B strain fermentation medium is prepared from 5 to 100 g/L of casein peptone, 0.5 to 80 g/L of yeast powder, 0.5 to 80 g/L of polyvalent peptone, 0.5 to 20 g/L of sodium chloride, 0.5 to 20 g/L of sodium glutamate, 0.5 to 30 g/L of glucose, 0.5 to 30 g/L of fish meal peptone, 5 to 100 mL/L of chicken serum, 0.5 to 50 mL/L of microelement solution and 1 to 30 mL/L of NAD (Nicotinamide Adenine Dinucleotide) solution. The haemophilus paragallinarum B strain fermentation medium disclosed by the invention contains all nutritional ingredients which are needed for growth of haemophilus paragallinarum B strains, growth and metabolism of the strains are facilitated, the adaption period of thallus is shortened, the adaptive capacity of the thallus after inoculation is strong, the fermentation period is short, the viable bacteria quantity during harvesting is high, and the haemophilus paragallinarum B strain fermentation medium is suitable for large-scale application and has a wide prospect.

A novel peptide obtained from Haemophilus paragallinarum has been found useful for preventing avian infectious coryza. This polypeptide induces production of hemagglutination-inhibition antibody and prevents infection and onset of avian infectious coryza. The invention further provides a gene coding for the polypeptide, a recombinant vector for expression of this gene, a host transformed with this vector, a process for preparing the polypeptide in a host, a vaccine for avian infectious coryza comprising the polypeptide as an active ingredient, a monoclonal antibody obtained using the polypeptide as an immunogen, and a diagnostic agent and a therapeutic agent for avian infectious coryza using the peptide and the antibody.

The invention discloses a haemophilus paragallinarum and application thereof. The haemophilus paragallinarum is haemophilus paragallinarum HNHpg1, the preservation number is CGMCC NO:13859, the preservation date is May 26th, 2017, the preservation place is China General Microbiological Culture Collection Center and the preservation address is Beijing, China. The haemophilus paragallinarum HNHpg1 is separated from secreta of sinus infraorbitalis of a laying hen suffering from typical respiratory symptoms and head and facial swelling, has stable biological characteristics, has stronger pathogenicity on chicks, can result in morbidity and death of the chicks and has good immunogenicity. A vaccine prepared by utilizing the haemophilus paragallinarum HNHpg1 disclosed by the invention is safe and reliable and has a better protective effect on chicken infectious rhinitis.

The invention relates to a culture medium efficiently culturing chicken coryza haemophilus paragallinarum and a preparation method and application of the culture medium. The culture medium is scientific and reasonable in formula, chick embryo allantoic fluid replaces serum, the cost can be greatly lowered, growth of the culture medium culturing the chicken coryza haemophilus paragallinarum is facilitated, compared with other culture media sold on the market, the cost is lowered by 20%-30%, the number of growing chicken coryza haemophilus paragallinarum thalli is increased by 5%-500%, and the culture medium can be widely used for culturing the chicken coryza haemophilus paragallinarum.

The invention relates to a chicken feed additive, applications thereof and a chicken feed, and belongs to the technical field of feeds. The chicken feed additive comprises the following raw materials:20-40 parts by weight of modified chitosan, 5-7 parts by weight of sodium diacetate, 2-2.5 parts by weight of chlorogenic acid, 3-4 parts by weight of grapefruit peel, 4-6 parts by weight of Indian mock strawberries and 8-12 parts by weight of schefflera octophylla. The modified chitosan is obtained by modifying chitosan by serine. The chicken feed additive is reasonable in formula, safe, non-toxic, and low in cost, and can effectively kill a plurality of pathogenic bacteria in chicken eyes in addition to aspergillus, and reduce the prevalence of chicken eye diseases. The additive can be usedfor preparing the chicken feed for treating chicken eye diseases, and can reduce the prevalence of chickens by killing aspergillus, salmonella, haemophilus paragallinarum and escherichia coli. The chicken feed containing the chicken feed additive is safe to eat and conducive to improving the breeding efficiency.

The invention relates to application of dextran-combined astragalus polysaccharide in detection of haemophilus paragallinarum causing infectious rhinitis of chicken as well as a kit and a detection method of the dextran-combined astragalus polysaccharide. By adding dextran and astragalus polysaccharide in a loop-mediated isothermal amplification system until the final concentration is 0.1-0.5mol/L (calculated by glucose), the stability of polymerase is improved, expensive 'polymerase' and 'glycine betaine' in a common LAMP kit can be partly replaced, a high-content GC fragment can be promoted to amplify very well, the detection reaction time can be reduced to 20-30 minutes, the detection cost can be reduced, meanwhile, the detection period is shortened, and the dextran-combined astragalus polysaccharide has important significance for loop-mediated isothermal amplification in the detection of the haemophilus paragallinarum causing the infectious rhinitis of chicken.

The invention discloses a preparation method and application of a haemophilus paragallinarum trivalent genetic engineering subunit vaccine, and belongs to the field of veterinary biological products. According to the invention, the fusion protein rApg-ABC capable of simultaneously preparing three serotypes of haemophilus paragallinarum A, B and C is constructed and expressed. According to the invention, an rApg-ABC fusion protein gene is connected to a pFastBac1 vector, and the rApg-ABC fusion protein gene is transformed into a DH10Bac competent cell to obtain a recombinant rod grain. And transfecting the recombinant baculovirus to sf9 cells, culturing to obtain recombinant baculovirus, inoculating the recombinant baculovirus into suspended insect cells HF to efficiently express antigen protein, extracting, purifying, carrying out BEI inactivation, adding an adjuvant, and emulsifying to obtain the vaccine. The preparation method is simple, can prepare a large amount of antigen protein, is short in time consumption and high in expression quantity, greatly reduces the production cost, and is beneficial to large-scale production.