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Antigen simulating epipeptide of A type parachicken blood phili bacillus and its use

A technology of Haemophilus gallinarum and mimetic epitopes, which is applied in the field of short peptides imitating epitopes of Haemophilus paragallinarum, achieves good economic benefits, simplifies the seedling production process, and reduces the effects of vaccine chemical residues

Inactive Publication Date: 2006-01-25
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on Haemophilus paragallinitis epitope vaccine of chicken rhinitis is rarely reported. If the HPG neutralizing antigenic epitope can be found out, its genetic engineering vaccine will be developed, which will reduce the influence of endotoxin and reduce the production capacity. Seedling costs, thereby reducing economic losses

Method used

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  • Antigen simulating epipeptide of A type parachicken blood phili bacillus and its use
  • Antigen simulating epipeptide of A type parachicken blood phili bacillus and its use
  • Antigen simulating epipeptide of A type parachicken blood phili bacillus and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Screening and sequence analysis of Haemophilus paragallinarum antigen mimotope

[0030] Experimental Materials

[0031] 1 Phage Random Peptide Library Kit

[0032] Linear 12-peptide library displayed on phage surface capsid protein III (Ph.D.-12 TM Phage Peptide Library PeptideLibrary Kit) kit was purchased from New England Biolabs, USA. The titer of the peptide library was 4×10 12 pfu / ml, random diversity 1.9×10 9 ; The genotype of the recipient strain E.coli ER2537 is F'lac q Δ(lacZ)M15proA + B + fhuA2 supE thiΔ(lac-proAB)Δ(hsdMS-mcrB)5(r k - m k - McrBC - ). DNA automatic sequencing primer (-96gIII sequencing primer): 5'-CCCTCATAGTTAGCGTAACG-3'

[0033] 2 Antisera of Haemophilus paragallinarum type A and type C and chickens artificially infected with HPg A and HPg C

[0034] Haemophilus paragallinarum type A: 0083 strains; Haemophilus paragallinarum type C: 668 strains and Modesto strain are all preserved by the Animal Husbandry and Veterina...

Embodiment 2

[0114] Example 2: Application of Mimotope Fusion Peptides in Vaccines

[0115] Experimental Materials

[0116] Host bacteria: GI826(F - ,lacI q ,ampC∷P trp cI, ΔfliC, ΔmotB, eda::Tn10), products of Invitrogen;

[0117] Plasmid: pFliTrx Invitrogen;

[0118] main reagent

[0119] Restriction endonuclease: ApaI, product of XhoI TaKaRa company;

[0120] T 4 DNA ligase: TaKaRa company product;

[0121] Taq polymerase: product of TaKaRa Company.

[0122] Related media

[0123] 10 x M9 Salt: Na 2 HPO 4 60g, KH 2 PO 4 30g, NaCl 5g, NH 4 Dissolve 10g of Cl in 900ml of deionized water and adjust the pH to 7.4 with 10M NaOH;

[0124] IMC medium: containing 1×M9 salt solution, 0.2% casein acid hydrolyzate, 0.5% glucose, 1mmol / L MgCl 2 ;

[0125] RM medium: 1×M9 salts, 2% tyrosine, 1% glycerol, 1mM MgCl 2 ;

[0126] SOC medium: tryptone 20g, yeast extract 5g, NaCl 0.5g, 2.5mmol / L KCl, 10mmol / LMgCl 2 , 20mmol / L glucose.

[0127] equipment

[0128] BIO-Rad Gene Pulser...

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Abstract

The invention relates to the antigen imitated epitope peptide of Haemophilus paragallinarum serovar A in China and its application, wherein its amino acid sequence is represented by the general formula of X1X2X3X4AX5DPVX6X7X8, wherein A represents alanine residue, D represents aspartate residue, P represents proline residue, V represents valine residue, X1-X8 represents any amino acids. The invention also discloses the use of the epitope peptide of Haemophilus paragallinarum serovar A -type in China as active ingredient in the preparation of vaccine for treating chicken infectious rhinitis.

Description

technical field [0001] The present invention relates to short peptides simulating Haemophilus paragallinarum antigenic epitopes, in particular to a group of short peptides capable of simulating type A Haemophilus paragallinarum antigenic epitopes, and the use of this group of short peptides in the development of chicken infectious rhinitis vaccine Applications. technical background [0002] Haemophilus paragallinarum (Hpg) is a pathogenic bacterium that causes infectious rhinitis in chickens. It can be divided into three serotypes: A, B, and C. Types A and C are common in my country now. Chicken infectious rhinitis is an acute respiratory infectious disease. Its main hazards are the decrease in egg production of laying hens and poor growth of reared chickens. It has been reported in the literature that infectious rhinitis caused by Hpg can reduce egg production by 40%, causing serious economic losses. [0003] At present, the prevention and treatment of chicken infectious...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61K39/07A61P31/04
Inventor 王宏俊高亚萍龚玉梅柳川孙惠玲安烨张培君
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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