Preparation method of chicken infectious rhinitis and mycoplasma gallisepticum bivalent lipid inactivated vaccine
A technology for chicken infectious rhinitis and mycoplasma gallisepticum, applied in the direction of microorganism-based methods, biochemical equipment and methods, medical preparations containing active ingredients, etc., can solve the problem of short duration of immunity, high lipid content, manufacturing High cost and other problems, to achieve the effect of improving physical stability, less injection stress, and reducing production costs
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Embodiment 1
[0034] Embodiment 1, the preparation of chicken infectious rhinitis, mycoplasma gallisepticum dual lipid inactivated vaccine, is carried out according to the following steps:
[0035] (1) The preparation of type A Haemophilus paragallinarum inactivated antigen bacterial liquid is prepared according to the following steps:
[0036] Preparation of primary strains: Streak inoculation of Haemophilus paragallinarum type A strains on chicken broth agar plates, in CO 2 The volume content is 5%, the temperature is 16h in the candle jar of 37 ℃, selects the 5 typical colonies that are 1mm in diameter that meet the characteristics of the strain, dilute it with 10ml sterilized chicken broth and inoculate it in the yolk sac of 6-day-old SPF chicken embryo. Inoculate 0.2ml of each embryo, continue to incubate at 37°C, collect the yolk fluid of dead embryos within 30 hours, and use it as the first-class strain after pure inspection confirms that there are no miscellaneous bacteria.
[0037...
Embodiment 2
[0053] Embodiment 2, the preparation of chicken infectious rhinitis, mycoplasma gallisepticum dual lipid inactivated vaccine, is carried out according to the following steps:
[0054] (1) The preparation of type A Haemophilus paragallinarum inactivated antigen bacterial liquid is prepared according to the following steps:
[0055] Preparation of primary strains: Streak inoculation of Haemophilus paragallinarum type A strains on chicken broth agar plates, in CO 2The volume content is 5%, and the temperature is 18h in the environment of 37 ℃, selects 3 typical colonies with a diameter of 1mm that meet the characteristics of the strain and dilutes it with 10ml sterilized chicken broth and inoculates it in the yolk sac of 7-day-old SPF chicken embryos. Embryos were inoculated with 0.2ml, and incubated at 37°C, and the yolk fluid of dead embryos within 30 hours was collected. After pure inspection confirmed that there were no miscellaneous bacteria, it was regarded as the first-cla...
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