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Preparation method of chicken infectious rhinitis and mycoplasma gallisepticum bivalent lipid inactivated vaccine

A technology for chicken infectious rhinitis and mycoplasma gallisepticum, applied in the direction of microorganism-based methods, biochemical equipment and methods, medical preparations containing active ingredients, etc., can solve the problem of short duration of immunity, high lipid content, manufacturing High cost and other problems, to achieve the effect of improving physical stability, less injection stress, and reducing production costs

Inactive Publication Date: 2012-04-11
SHANDONG BINZHOU BOLAIWEI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vaccines made using this method are effective, but are high in lipid content, expensive to manufacture, and do not last long in immunity

Method used

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  • Preparation method of chicken infectious rhinitis and mycoplasma gallisepticum bivalent lipid inactivated vaccine
  • Preparation method of chicken infectious rhinitis and mycoplasma gallisepticum bivalent lipid inactivated vaccine
  • Preparation method of chicken infectious rhinitis and mycoplasma gallisepticum bivalent lipid inactivated vaccine

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Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1, the preparation of chicken infectious rhinitis, mycoplasma gallisepticum dual lipid inactivated vaccine, is carried out according to the following steps:

[0035] (1) The preparation of type A Haemophilus paragallinarum inactivated antigen bacterial liquid is prepared according to the following steps:

[0036] Preparation of primary strains: Streak inoculation of Haemophilus paragallinarum type A strains on chicken broth agar plates, in CO 2 The volume content is 5%, the temperature is 16h in the candle jar of 37 ℃, selects the 5 typical colonies that are 1mm in diameter that meet the characteristics of the strain, dilute it with 10ml sterilized chicken broth and inoculate it in the yolk sac of 6-day-old SPF chicken embryo. Inoculate 0.2ml of each embryo, continue to incubate at 37°C, collect the yolk fluid of dead embryos within 30 hours, and use it as the first-class strain after pure inspection confirms that there are no miscellaneous bacteria.

[0037...

Embodiment 2

[0053] Embodiment 2, the preparation of chicken infectious rhinitis, mycoplasma gallisepticum dual lipid inactivated vaccine, is carried out according to the following steps:

[0054] (1) The preparation of type A Haemophilus paragallinarum inactivated antigen bacterial liquid is prepared according to the following steps:

[0055] Preparation of primary strains: Streak inoculation of Haemophilus paragallinarum type A strains on chicken broth agar plates, in CO 2The volume content is 5%, and the temperature is 18h in the environment of 37 ℃, selects 3 typical colonies with a diameter of 1mm that meet the characteristics of the strain and dilutes it with 10ml sterilized chicken broth and inoculates it in the yolk sac of 7-day-old SPF chicken embryos. Embryos were inoculated with 0.2ml, and incubated at 37°C, and the yolk fluid of dead embryos within 30 hours was collected. After pure inspection confirmed that there were no miscellaneous bacteria, it was regarded as the first-cla...

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Abstract

The invention provides a preparation method of a chicken infectious rhinitis and mycoplasma gallisepticum bivalent lipid inactivated vaccine, belonging to the technical field of biological products for animals. The preparation method is implemented by the following steps of: preparing a type A haemophilus paragallinarum inactivated antigen bacterial liquid, a type C haemophilus paragallinarum inactivated antigen bacterial liquid and a mycoplasma gallisepticum antigen liquid; uniformly mixing the type A haemophilus paragallinarum inactivated antigen bacterial liquid with the type C haemophilus paragallinarum inactivated antigen bacterial liquid in the volume ratio of 1:1 to obtain a mixed haemophilus paragallinarum inactivated antigen bacterial liquid; uniformly mixing the mixed haemophilus paragallinarum inactivated antigen bacterial liquid with the mycoplasma gallisepticum antigen liquid in the ratio of 1:1-1:1.5, pouring into an emulsifying tank and stirring; slowly adding poplar bark lipid till the final volume percentage concentration of the poplar bark lipid in the mixture is 2.0-3.8 percent; and continually stirring and emulsifying for 30-60 minutes. The vaccine has the advantages of easiness for absorbing, no toxic or side effect, short immunogenic time, long immune duration, good immune effect and capability of effectively preventing chicken infectious rhinitis and mycoplasma gallisepticum.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological product preparation and poultry disease prevention and control, and in particular relates to a preparation method of a chicken infectious rhinitis and mycoplasma gallisepticum dual lipid inactivated vaccine. Background technique [0002] Avian infectious rhinitis is an acute respiratory infectious disease caused by Haemophilus paragallinarum. The main features are facial swelling, nasal serous to mucous secretions and conjunctivitis. The incidence rate is very high and it is distributed worldwide. Mycoplasma gallisepticum (MG) is a chronic respiratory infectious disease of chickens, characterized by wide distribution and high infection rate, characterized by rales, cough, runny nose and sinus swelling in the respiratory tract. Infectious rhinitis or Mycoplasma gallisepticum alone can kill chickens, but what is more harmful is the secondary or concurrent infection of various bacterial...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/116C12N1/20A61P31/04A61K39/102A61K39/02C12R1/21C12R1/35
Inventor 杨灵芝李香云董新荣吕茂杰贾红梅陈申秒
Owner SHANDONG BINZHOU BOLAIWEI BIOTECH
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