31 results about "Mycoplasma equirhinis" patented technology

The invention relates to a preparation method of a mycoplasma gallisepticum and mycoplasma synoviae bivalent inactivated vaccine. The method comprises the steps as follows: a mycoplasma gallisepticum virulent CR strain and a mycoplasma synoviae HN01 strain which have good immunogenicity are inoculated on a proper culture medium for cultivation, so that a culture is acquired; and the culture is inactivated through a formaldehyde solution and then is mixed with an oil emulsion adjuvant and emulsified, so that the mycoplasma gallisepticum and mycoplasma synoviae bivalent inactivated vaccine is prepared. The mycoplasma gallisepticum and mycoplasma synoviae bivalent inactivated vaccine is used for preventing mycoplasma gallisepticum and mycoplasma synoviae diseases, and can realize immunization, prevent two pathogens at the same time and reduce the workload of immunization; and the prepared vaccine is stable in performance, good in immune effect, and more suitable for actual production of China.

The invention relates to compound tylosin injection for animals and a preparation method thereof. The injection consists of tylosin, doxycycline hydrochloride, trimethoprim, aminophylline, dexamethasone sodium phosphate, organic solvent and injection water. As a dedicated compound preparation for animals, the injection has special effect for treating respiratory tract infection caused by livestock and poultry sensitive bacteria and mycoplasmosis of pigs and poultry. The method has the advantages of convenient use, short course of treatment, low drug resistance and the like.

The invention discloses a traditional Chinese medicine for preventing and controlling respiratory diseases of animals, which is prepared from the following components in percentage by weight: 9 to 17 percent of liquoric root, 6 to 14 percent of gypsum, 4 to 12 percent of root of large-flowered skull, 3 to 11 percent of honeysuckle, 3 to 11 percent of sweeping forsythia, 2 to 10 percent of root bark of white mulberry, 2 to 10 percent of dried tangerine, 2 to 10 percent of tangerine seed, 2 to 10 percent of hawthorn, 2 to 10 percent of loguat leaf, 2 to 10 percent of root of straight ladybell, 3 to 7 percent of whiteflower hogfennel root, 3 to 7 percent of semen armeniacae amarae, 3 to 7 percent of rhizoma anemarrhenae, 1 to 3 percent of bryozoatum and 1 to 3 percent of borneol. By adopting the formula, from the screening of raw materials to the extraction and proportioning of active ingredients, a slow release / controlled release and targeting drug delivering technology is applied, so the medicine can effectively and quickly reach lesion sites, has the advantages of quick response, short treatment time, small side effect, convenient use, strong pertinence and high protectiveness, maintains the blood concentration in an effective treatment range, and effectively prevents and controls non typical Newcastle disease, bird flu, infectious laryngotracheitis, infectious bronchitis, colibacillus, mycoplasmosis and unexplained animal diseases such as head and face swelling and expiratory dyspnea.

The invention provides a preparation method of a chicken infectious rhinitis and mycoplasma gallisepticum bivalent lipid inactivated vaccine, belonging to the technical field of biological products for animals. The preparation method is implemented by the following steps of: preparing a type A haemophilus paragallinarum inactivated antigen bacterial liquid, a type C haemophilus paragallinarum inactivated antigen bacterial liquid and a mycoplasma gallisepticum antigen liquid; uniformly mixing the type A haemophilus paragallinarum inactivated antigen bacterial liquid with the type C haemophilus paragallinarum inactivated antigen bacterial liquid in the volume ratio of 1:1 to obtain a mixed haemophilus paragallinarum inactivated antigen bacterial liquid; uniformly mixing the mixed haemophilus paragallinarum inactivated antigen bacterial liquid with the mycoplasma gallisepticum antigen liquid in the ratio of 1:1-1:1.5, pouring into an emulsifying tank and stirring; slowly adding poplar bark lipid till the final volume percentage concentration of the poplar bark lipid in the mixture is 2.0-3.8 percent; and continually stirring and emulsifying for 30-60 minutes. The vaccine has the advantages of easiness for absorbing, no toxic or side effect, short immunogenic time, long immune duration, good immune effect and capability of effectively preventing chicken infectious rhinitis and mycoplasma gallisepticum.

An H95 attenuated mycoplasma synoviae strain is obtained through in-vitro subculture in order to research an attenuated vaccine capable of preventing mycoplasma synoviae. The H95 strain and a white oil adjuvant are mixed in the proportion of 1:1 to prepare the attenuated vaccine, and the immune effect of the strain on chickens is researched by immunizing the chickens with different immune doses; animal infection research is carried out through a pathogenicity wild strain, so that the prevention effect of the attenuated vaccine prepared from the H95 attenuated strain on mycoplasma synoviae is verified. The invention provides a preparation method of the attenuated vaccine capable of preventing mycoplasma synoviae and an effective immunizing dose. A scientific basis is provided for clinicallypreventing mycoplasma synoviae.

The invention relates to a traditional Chinese medicine extract having poultry and livestock mycoplasma resistant activity. The traditional Chinese medicine extract contains any one or more of the following components: 1-512 mu g / mL of nitidine chloride, 1-512 mu g / mL of ethoxychelerythrine, 1-512 mu g / mL of chelerythrine, 1-512 mu g / mL of sanguinarine, 1-512 mu g / mL of coptisine, 1-512 mu g / mL of palmatine, 1-512 mu g / mL of epiberberine, and 1-512 mu g / mL of glycyrrhetinic acid. The invention further provides a traditional Chinese medicine composition having poultry and livestock mycoplasma resistant activity. Both the traditional Chinese medicine extract and the traditional Chinese medicine composition provided by the invention have the stronger effect for inhibiting poultry and livestock mycoplasma activity in vitro, are safe, green and free from medicine residue, and can be applied to treating and preventing poultry and livestock mycoplasma diseases.

The invention relates to the field of traditional Chinese medicine for treating poultry diseases, in particular to traditional Chinese medicine for treating poultry synovial bursa mycoplasmosis and apreparation method thereof. The traditional Chinese medicine for treating poultry synovial bursa mycoplasmosis is prepared from the following raw materials in parts by weight: 10 to 20 parts of radixcodonopsis, 10 to 20 parts of liquorice roots, 15 to 25 parts of rhizoma zingiberis, 10 to 20 parts of radix aconiti carmichaeli, 15 to 30 parts of cassia twigs, 15 to 25 parts of poria cocos, 15 to 25 parts of bighead atractylodes rhizome, 10 to 20 parts of rhizoma sinomeni cauliset, 15 to 25 parts of radix clematidis, 12 to 20 parts of Chinese herbaceous peony flowers, 10 to 20 parts of medicinal cyathula roots and 15 to 25 parts of caulis spatholobi. The traditional Chinese medicine is put into a traditional Chinese medicine extraction tank; water is added for decoction and boiling; backflow extraction is performed twice; the decoction solutions in the two times are merged; then, filtering is performed; after filter liquid is concentrated, the materials are cooled to the room temperature; the traditional Chinese medicine liquid is obtained. The traditional Chinese medicine is scientifically matched according to the monarch, minister, assistant and guide principle, and achieves the effects of invigorating the spleen to eliminate damp, removing cold to eliminate damp and clearing and activating the channels and collaterals. The traditional Chinese medicine has the advantages thatthe use of antibiotics is reduced; the green and antibiotic-free culture is practiced; the safety and the health of poultry meat products are guaranteed.

A veterinary Chinese medicine for treating the mixed infection of mycoplasma disease and enteritis of domestic fowls is proportionally prepared from coptis root, scutellaria root, phellodendron bark and capejasmine fruit.

The invention provides a compound propolis composition for treating chicken mycoplasmosis and a preparation method thereof, which relate to the field of animal medicines. The composition is made by evenly mixing of the following components, parts by weight, 15-35 parts of propolis, 15-30 parts of pericarpium citri reticulatae, 15-30 parts of radix sophorae flavescentis, 15-40 parts of houttuynia cordata, 15-40 parts of scutellaria baicalensis, 15-30 parts of rheum officinale, 15-40 parts of liquorice, 15-40 parts of platycodon grandiflorum, 15-30 parts of rhizoma atractylodis, 15-30 parts of radix curcumae and 15-40 parts of gentiana scabra bunge. The compound propolis composition for treating the chicken mycoplasmosis has the advantages of being reasonable in compound, simple in preparation and free of toxic and side effects, pollution, drug residue, drug resistance, superfine in smashing, high in biological availability and very remarkable in curative effect. Clinic application proves that the compound propolis composition has especially good effects on treating of the chicken mycoplasmosis.

The invention discloses a new application of p-hydroxyl cinnamic acid in preparing a medicament for preventing and curing viral diseases or bacterial diseases of livestock and poultries. The product can prevent and cure viral and bacterial diseases of the livestock and the poultries and improve the production performance of the livestock and the poultries. Proved by in vivo and in vitro experiments, the p-hydroxyl cinnamic acid has the effects of preventing and curing diseases caused by mycoplasma gallisepticums, cow escherichia coli, and the like. Proved by in vivo experiments, the p-hydroxyl cinnamic acid has the effects of preventing and curing Fabricius bursal viral diseases of the livestock and the poultries, avian mycoplasmosis and avian influenza H9 and H5 subtype virus infections.

The invention discloses a new application of p-hydroxyl cinnamic acid in preparing a medicament for preventing and curing viral diseases or bacterial diseases of livestock and poultries. The product can prevent and cure viral and bacterial diseases of the livestock and the poultries and improve the production performance of the livestock and the poultries. Proved by in vivo and in vitro experiments, the p-hydroxyl cinnamic acid has the effects of preventing and curing diseases caused by mycoplasma gallisepticums, cow escherichia coli, and the like. Proved by in vivo experiments, the p-hydroxyl cinnamic acid has the effects of preventing and curing Fabricius bursal viral diseases of the livestock and the poultries, avian mycoplasmosis and avian influenza H9 and H5 subtype virus infections.

The invention discloses new application of p-hydroxycinnamic acid to preparation of medicines for preventing and treating viral disease or bacterial disease of livestock and poultry. The product can prevent and treat viral disease or bacterial disease of livestock and poultry and can improve production performance of livestock and poultry. In vivo and in vitro tests show that the p-hydroxycinnamic acid can prevent and treat diseases caused by mycoplasma gallisepticum, bovine colibacillosis and the like. The in vivo test shows that the p-hydroxycinnamic acid can prevent and treat poultry bursafabricius viral disease, avian mycoplasmosis and bird flu H9 and H5 subtype virus infection.

The invention discloses a feed additive for quails in the egg producing period and a preparation method of the feed additive. The feed additive is prepared from medicated leaven, snow lotus herb, herba cichorii, tinospora sinensis, fructus aurantii immaturus, barberry roots, herb of rupestrine dragonhead, leaves of evodia lepta (spr.) Merr., fructus mori, coromadel coast falsemallow herb, rhizoma polygonati odorati, ganoderma, all-grass of downy ground-cherry, aboveground part of polygonum orientale L., roots of twelve stamen melastoma, roots, leaves and fruits of zanthoxylum avicennae (Lam.)DC., herb of hokker winghead, pinus tabulaeformis Carr., seeds of whiteflower cacalia and boor's mustard herb. The feed additive for the quails in the egg producing period has the advantages that the feed additive can meet nutritional requirements of the quails, the egg yield of the quails can be obviously increased, food and dyspepsia diseases, pullorum diseases, ulcerative enteritis, mycoplasmosis, bronchitis, constipation, soft-shelled egg laying diseases and the like can be well prevented, the obvious superiority is achieved, the raw materials are all natural products, and the feed additive has the advantages of being high in safety, free of drug residues and toxic and side effects and the like.

The invention belongs to the technical field of chemical synthesis, and in particular relates to water-soluble sarafloxacin mesylate and a preparation method thereof. The sarafloxacin mesylate provided by the invention has the structure shown in the specification. The invention also provides the preparation method of the sarafloxacin mesylate, which comprises the following steps of: dissolving sarafloxacin hydrochloride into water and separating out sarafloxacin solid by adjusting pH; and dissolving the sarafloxacin solid into an organic solution and reacting with methanesulfonic acid to obtain the sarafloxacin mesylate target product. The water-soluble sarafloxacin mesylate and the preparation method thereof are used for solving the technical problem about low water dissolubility of the sarafloxacin and the sarafloxacin hydrochloride. The water solubility of the target product is obviously higher than that of the traditional sarafloxacin hydrochloride raw material; the target product has the advantages of wide antibacterial spectrum, high antimicrobial activity and the like; and the target product is stronger than the sarafloxacin hydrochloride in the aspects of prevention and treatment on mycoplasmosis and treatment on streptococcicosis and colibacillosis, and has a good application prospect.

The invention discloses a medicinal preparation for preventing and curing mycoplasmosis of livestock and improving non-specific immunity. The medicinal preparation is prepared from the following materials in parts by weight: 15-30 parts of tilmicosin, 4-10 parts of epimedium folium, 4-10 parts of radix bupleuri, 8-18 parts of folium eriobotryae, 3-6 parts of fructo-oligose, 20-30 parts of medical stone and 15-25 parts of anhydrous dextrose. The invention further discloses a preparing method of the medicinal preparation. According to the medicinal preparation, all the components of the medicinal preparation are compatible and have the effects of promoting the tilmicosin to repair immune cells and improving the functions of the immune cells. Meanwhile, the growth of normal flora in intestines is protected and promoted, and the organism immunity function is enhanced. In addition, the preparing method is convenient to operate and easy to popularize and apply.

The invention relates to feed for pheasants which are 40-60 days old. The feed is prepared from corn, fish powder, wheat bran, bone powder, feather meal, artemisia seleirgensis, thesium chinense, feather cockscomb seeds, purslane, pine needle powder, laminaria japonica powder, lactoferrin, stachyose, sodium caseinate, calcium hydrophosphate, zeolite powder, clove oil, origanum oil and blood meal. The feed is balance in formula nutrient and can meet the nutrient requirements for normal growth of the pheasants which are 40-60 days old. The feed can also prevent the young pheasants from being infected by coccidiosis, mycoplasmosis and colibacillosis, and increases the growth speed of the pheasants which are 40 days old.

The invention belongs to the technical field of veterinary drugs, and relates to a traditional Chinese medicine composition for treating synovial sac mycoplasmosis of laying hens and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 70-90 parts of cassia twig, 50-70 parts of Chinese peony, 10-30 parts of liquorice, 30-50 parts of ephedra, 40-60 parts of long pepper, 40-60 parts of largehead atractylodes rhizomes, 70-90 parts of gypsum, 30-50 parts of common anemarrhena rhizomes, 30-50 parts of divaricatesaposhnikovia roots, 10-30 parts of common monkshood branched roots, 20-40 parts of papaya, 20-40 parts of largeleaf gentian roots and 20-40 parts of kadsura pepper stems. The composition is a pure traditional Chinese medicine preparation, can reduce the incidence rate of synovial sac mycoplasmosis of laying hens, obviously improves symptoms such as arthrocele, emaciation and the like, is safe, has no toxic or side effect and no palatability problem, and has good market application value.

The invention provides Chinese veterinary medicine composition used for preventing and treating poultry mycoplasmosis and a preparation method thereof. The Chinese veterinary medicine composition mainly comprises the following components in parts by weight: 10-20 parts of dandelion, 10-20 parts of baicalein, 10-20 parts of honeysuckle, 10-20 parts of ephedra, 10-20 parts of perilla fruit, 10-20 parts of common coltsfoot flower, and 5-10 parts of liquorice. The Chinese veterinary medicine composition used for preventing and treating poultry mycoplasmosis, which is prepared by the preparation method of the invention, has the functions of clearing away heat and toxic materials, moistening lung to arrest cough, relieving asthma, eliminating phlegm, diminishing inflammation, relieving pain, and can enhance the immunity of poultry and the disease-resisting capability of the organism. Moreover, the Chinese veterinary medicine composition has advantages of safety, no medicine residue, and no side effects, and further has the advantages that the Chinese veterinary medicine composition is favorable to take orally and absorb and simple in process. The Chinese veterinary medicine composition is suitable for industrial production.

The invention relates to a traditional Chinese medicine composition for treating livestock and poultry upper respiratory tract infection and a preparation method thereof. The composition comprises the components of, by weight, 5-10 parts of Schizonepeta, 5-15 parts of radix sileris, 10-15 parts of Folium Isatidis, 5-10 parts of chrysanthemum, 10-15 parts of honeysuckle, 10-20 parts of Platycodon grandiflorum, 10-20 parts of forsythia, 5-10 parts of radices cyathulae, 5-10 parts of mint, 5-10 parts of licorice and 5-10 parts of bitter apricot kernel. The composition of the invention has the advantages of scientific compatibility, simple process, easiness for usage, low cost, no drug residue and no toxic or side effects, and effective enhancement of immunity, and has high kill ratio on bacteria, morbus virosus and mycoplasmosis, and no drug resistance.

The invention discloses an immunocolloidal gold test paper for rapidly detecting mycoplasma bovis and a preparation method thereof. The bottom layer of the test paper is a support plate, the support plate is sequentially adhered with a sample pad, a bonding pad, an NC film and an absorption pad which are closely connected. A mycoplasma bovis P33 monoclonal antibody with a colloidal gold mark is absorbed on the bonding pad, a rabbit anti-mycoplasma bovis P33 polyclonal antibody is sprayed on the NC film to take as a detection line and a sheep anti-BALB / C mouse IgG antibody is sprayed on the NCfilm to take as a quality control line, the line widths of the detection line and the quality control line are 1mm, the distance of the detection line and the quality control line is 5mm, the detection line and the quality control line are located at the center of the NC film, and the amino acid sequence of the mycoplasma bovis P33 protein is as shown such as SEQ ID NO.1. The test paper can be used for rapid detection of the mycoplasma bovis antigen to generate a result after 5-10min, the sensitivity is 5 ng / mL, the sample amount is few, cross reaction is not needed, the flexibility is high, the requirements of low cost and rapid field diagnosis can be met, and the detection efficiency can be greatly improved. The immunocolloidal gold test paper for rapidly detecting mycoplasma bovis and the preparation method thereof can develop important effects in the prevention and control of bovine mycoplasmosis and have potential promotion and application values.

The invention relates to a preparation method of a mycoplasma gallisepticum and mycoplasma synoviae bivalent inactivated vaccine. The method comprises the steps as follows: a mycoplasma gallisepticum virulent CR strain and a mycoplasma synoviae HN01 strain which have good immunogenicity are inoculated on a proper culture medium for cultivation, so that a culture is acquired; and the culture is inactivated through a formaldehyde solution and then is mixed with an oil emulsion adjuvant and emulsified, so that the mycoplasma gallisepticum and mycoplasma synoviae bivalent inactivated vaccine is prepared. The mycoplasma gallisepticum and mycoplasma synoviae bivalent inactivated vaccine is used for preventing mycoplasma gallisepticum and mycoplasma synoviae diseases, and can realize immunization, prevent two pathogens at the same time and reduce the workload of immunization; and the prepared vaccine is stable in performance, good in immune effect, and more suitable for actual production of China.

The invention discloses a feed additive for treating avian mycoplasmosis. The feed additive comprises, by weight, 50-400 parts of radix ophiopogonis, 50-300 parts of radix adenophorae, 20-60 parts of radix glycyrrhizae, 50-250 parts of pollen, 250-500 parts of radix pulsatillae, 20-60 parts of radix paeoniae alba, 0.1-0.8 part of 6-benzylamino adenine, 0.5-2 parts of sodium polyacrylate, 20-150 parts of rhizoma anemarrhenae and 10-60 parts of fructus schisandrae. The feed additive can be added into feed according to a dosage of 3.0-7.0%. The feed additive for treating avian mycoplasmosis has the advantages that the feed additive is capable of inhibiting avian mycoplasmosis remarkably and increasing the survival rate of infected chickens obviously, and is low in operation requirements, easy to master and implement and harmless to chickens and feeders.

The invention relates to the field of an animal biological product, and particularly discloses a pig mycoplasma specific monoclonal antibody and its application in clinical detection; the pig mycoplasma specific monoclonal antibody is prepared by using mycoplasma hyopneumoniae as immunogen; a a pig mycoplasma specific monoclonal antibody hybridoma cell strain 2G2 with high stable secretion effect and strong specificity is successfully screened, the generated monoclonal antibody can generate immunoreaction with mycoplasma pneumonia and ureaplasma urealyticum at the same time; a pig mycoplasmosis detection kit is set up. The pig mycoplasma specific monoclonal antibody with strong specialty and high effect is used for preparing the pig mycoplasmosis immunologic diagnosis kit; the kit is sensitive, exact, and simple in operation, needs not to collect blood as sample, and not harms animals; the kit can quickly and exactly diagnose pig mycoplasma in early period; the kit can detect both of mycoplasmosis and ureaplasma urealyticum, thereby largely reducing cost and improving the detection effect.

The invention belongs to the technical field of chemical synthesis, and in particular relates to water-soluble sarafloxacin mesylate and a preparation method thereof. The sarafloxacin mesylate provided by the invention has the structure shown in the specification. The invention also provides the preparation method of the sarafloxacin mesylate, which comprises the following steps of: dissolving sarafloxacin hydrochloride into water and separating out sarafloxacin solid by adjusting pH; and dissolving the sarafloxacin solid into an organic solution and reacting with methanesulfonic acid to obtain the sarafloxacin mesylate target product. The water-soluble sarafloxacin mesylate and the preparation method thereof are used for solving the technical problem about low water dissolubility of the sarafloxacin and the sarafloxacin hydrochloride. The water solubility of the target product is obviously higher than that of the traditional sarafloxacin hydrochloride raw material; the target product has the advantages of wide antibacterial spectrum, high antimicrobial activity and the like; and the target product is stronger than the sarafloxacin hydrochloride in the aspects of prevention and treatment on mycoplasmosis and treatment on streptococcicosis and colibacillosis, and has a good application prospect.

The technical problem to be solved in the present invention is to rapidly and efficiently proliferate Mycoplasma gallisepticum so as to be used for rapid detection of Mycoplasma gallisepticum pathogen. In order to achieve the purpose, the prevent invention is achieved by adopting the following technical scheme that the Mycoplasma gallisepticum detection method is characterized in that a Mycoplasma gallisepticum liquid culture medium is prepared, wherein yeast extract powder, glucose and trypticase are concurrently used in a soybean papain digest, such that the actual passage time shortening effect is more than 10% of the passage time shortening effect of the only use of the traditional yeast extract powder, the shortest passage time can be as fast as 12-16 h, and the passage time is 18 h in most cases. According to the present invention, the Mycoplasma gallisepticum liquid culture medium is adopted as the main proliferation base material of the formula so as to rapidly detect the Mycoplasma gallisepticum pathogen, such that the effective reference is provided for the definite diagnosis of the disease and the epidemic prevention work of the chicken flocks.

The present invention relates to a chicken mycoplasmosis treating traditional Chinese medicine composition and a preparation method thereof, wherein the components comprise, by weight, 10-15 parts of tuckahoe, 10-15 parts of licorice root, 10-15 parts of radix paeoniae rubra, 10-15 parts of radix bupleuri, 10-15 parts of pericarpium citri reticulatae, 5-10 parts of nepeta cataria l, 5-10 parts of platycodon grandiflorus, 5-10 parts of gypsum, 10-15 parts of scutellaria baicalensis, and 10-15 parts of ephedra sinica stapf. The chicken mycoplasmosis treating traditional Chinese medicine composition of the present invention has effects of asthma relieving, cough stopping, lung heat clearing and phlegm dissipating, has characteristics of rapid effect, long acting time, low toxic-side effect and no drug residue, and is the ideal chicken mycoplasmosis treating traditional Chinese medicine composition.

The invention provides a compound propolis composition for treating chicken mycoplasmosis and a preparation method thereof, which relate to the field of animal medicines. The composition is made by evenly mixing of the following components, parts by weight, 15-35 parts of propolis, 15-30 parts of pericarpium citri reticulatae, 15-30 parts of radix sophorae flavescentis, 15-40 parts of houttuynia cordata, 15-40 parts of scutellaria baicalensis, 15-30 parts of rheum officinale, 15-40 parts of liquorice, 15-40 parts of platycodon grandiflorum, 15-30 parts of rhizoma atractylodis, 15-30 parts of radix curcumae and 15-40 parts of gentiana scabra bunge. The compound propolis composition for treating the chicken mycoplasmosis has the advantages of being reasonable in compound, simple in preparation and free of toxic and side effects, pollution, drug residue, drug resistance, superfine in smashing, high in biological availability and very remarkable in curative effect. Clinic application proves that the compound propolis composition has especially good effects on treating of the chicken mycoplasmosis.

The invention provides a specific primer and real-time fluorescent quantitative PCR detection method for detecting canine mycoplasmas, and belongs to the technical field of biology. According to the method, the specific primer is designed according to the sequence of a conserved region of a sialidase gene of the canine mycoplasmas published by Genebank, a target fragment is amplified and connectedto a pMD-18T vector, a recombinant standard plasmid is constructed and subjected to 10-fold dilution, a Ct value and a copy number have a good linear relationship, and the correlation coefficient is0.9947. The method is high in sensitivity, the minimum detection limit is 1.85*10<4> copy / microliter, and the sensitivity is improved by 100 times compared with that of common PCR detection; the specificity is good, and there is no specific amplification curve for three common mycoplasmas and common canine pathogens; the repeating stability is good, and the between-group and within-group variancecoefficients are both smaller than 0.5%. The method overcomes the defects that the identification time of traditional isolated culture is long, and a serological method easily causes a cross reactionand a conventional PCR false positive result, can be used for early diagnosis and detection of the canine mycoplasmas, and has important significance for the prevention and control of canine mycoplasmosis.