32 results about "Sarafloxacin" patented technology

A method for extracting and analyzing quinolone drugs by using a DPX tip-type dispersed solid-phase micro-extraction column relates to a method for detecting quinolone drug residues in animal-derivedfood. The invention aims to solve the problem that there is no effective method for detecting quinolone drugs in animal-derived food, especially measuring with quantitative high-precision, high-throughput and low-consumption in the prior art. According to the invention, a sample preparation and high performance liquid chromatography-tandem mass spectrometry method for detecting multi-residues of quinolones in animal-derived food is established. The method is suitable for the detection of single or multiple drug residues of enrofloxacin, ciprofloxacin, sarafloxacin, norfloxacin, ofloxacin, lomefloxacin and danofloxacin in animal-derived food.

The invention discloses a hybridoma cell strain capable of secreting monoclonal antibodies to quinolones and application of the monoclonal antibodies thereof. Ciprofloxacin (CIP) coupled with bovine serum albumin is used as an antigen to immunize BALB / c mice and cell fusion, screening and cloning are carried out so as to obtain one hybridoma cell strain 1F1 capable of stable passage and secretion of monoclonal antibodies (MAb) to quinolones, wherein, the accession number of the hybridoma cell strain 1F1 is CGMCC No. 5608. The titres of ascitic fluids of the 1F1 monoclonal antibodies are up to 10<-7>, and the type and the subclass of the monoclonal antibodies are IgG1 and kappa chain. According to indirect competitive ELISA analysis, the 1F1 monoclonal antibodies perform specific reactions to quinolones like ciprofloxacin, enrofloxacin, ofloxacin, danofloxacin, norfloxacin, enoxacin, marbofloxacin, sarafloxacin and difloxacin. An ELISA method, a kit and test paper for detecting residual of quinolones in food are developed by using the 1F1 monoclonal antibodies.

The invention belongs to the technical field of detection, and discloses a method for detecting the residue of four fluoroquinolone drugs in eggs. The method comprises the following steps: selecting phosphoric acid-acetonitrile as an extraction solution; selecting a degreasing method of water saturated n-hexane; selecting a Waters Oasis HLB column as a solid extraction column; selecting one fourthof extraction solution to pass through the column; selecting a flow phase ratio of 89 percent (0.05mol / L phosphoric acid / triethylamine): 11 percent (acetonitrile); wherein the retention time: enrofloxacin: 8.41+ / -0.5 min, ciprofloxacin: 5.41+ / -0.5 min, danofloxacin: 7.33+ / -0.5 min, and sarafloxacin: 12.53+ / -0.5 min. By adopting the method, the pretreatment time and consumable cost can be greatlysaved, the sensitivity and the recovery rate conform to the current veterinary drug residue detection standard in China, and the method is suitable for the mass sample analysis.

The invention discloses the application of artebenolide in eliminating the residues of quinolones (especially sarafloxacin) in cultured fish. On the one hand, artebolide can reduce the residual concentration of quinolones in cultured fish. On the one hand, artebolactone can significantly shorten the elimination half-life and drug withdrawal period of quinolones in cultured fish. Artebenolide can be used to accelerate the elimination of quinolone chemical drug residues in farmed fish, which is of great significance for ensuring the quality and safety of aquatic products and reducing the risk of human intake of aquatic products containing quinolone chemical drug residues.

The invention discloses a specific monoclonal antibody capable of recognizing fluoroquinolones. The monoclonal antibody is secreted by a hybridoma cell 5B11, which is preserved in the China Center for Type Culture Collection, and has a preservation number of CCTCC NO:C201149. The invention also discloses an enzyme-linked immunosorbent assay method and a kit for detecting, and application thereof to detection of residues of fluoroquinolones. Compared with prior art, the monoclonal antibody provided by the invention can identify 15 fluoroquinolones, especially can simultaneously identify Difloxacin and Sarafloxacin, thus the application range is wide. The enzyme-linked immunosorbent assay method and the kit established by the present invention have high sensitivity, high precision and good accuracy.

The invention provides a sarafloxacin hydrochloride water-soluble granule and a preparation method thereof, which solves the technical problems of the existing sarafloxacin hydrochloride drug with poor therapeutic effect and serious drug residue problem. It is composed of the following raw materials in parts by weight Composition: 15-45 parts of diethylenediamine, 150-220 parts of cyclodextrin-methyl vinyl ether / maleic anhydride copolymer, 100 parts of sarafloxacin hydrochloride, 635-735 parts of anhydrous glucose, appropriate amount of water; The invention also discloses a preparation method of sarafloxacin hydrochloride water-soluble granules: respectively weigh diethylenediamine, cyclodextrin-methyl vinyl ether / maleic anhydride copolymer, add to hot water and mix uniformly to obtain a mixture Weigh sarafloxacin hydrochloride and anhydrous glucose respectively, and mix uniformly to obtain mixture two; throw mixture two into a three-dimensional fluidized bed, and carry out fluidized bed granulation with mixture one as a binder; temperature, etc., to finally obtain sarafloxacin hydrochloride granules; which can be widely used in the technical field of veterinary medicine.

The invention discloses a sarafloxacin hydrochloride injection and a preparation method thereof. Each 100ml injection consists of the following components: 1-5g of sarafloxacin hydrochloride, 5-10g of a stabilizer, 15-50g of an organic solvent, and a metal ion chelate Mixture 0.01~0.05g, the dosage is enough to control the pH of the system as a pH regulator of 9.5~10.5, and the balance is water for injection; the stabilizer is one or more of ethanol, sodium acetate and sodium glycinate; the organic solvent is PEG- 400, one or more of propylene glycol, α-pyrrolidone and dimethylformamide. The present invention solves the problems that sarafloxacin hydrochloride is unstable, easily oxidized and discolored under high temperature conditions, has low solubility in water, and easily precipitates under low temperature conditions by adding a stabilizer and an organic solvent; and the trace amount of metal ions present in the water for injection catalyze the oxidation of the drug and play an anti-oxidation effect; the obtained sarafloxacin hydrochloride injection has good stability, simple preparation process and easy industrialization.

The invention discloses a method for extraction, enrichment and quantification of trace sarafloxacin on suspended particulate matters in water. The method comprises the following steps: filtering a target water sample by using a micro porous fiber membrane; collecting the filtered filter membrane, airing the filter membrane and then cutting into pieces, and putting the filter membrane pieces into a triangular flask; adding an extraction agent, sealing, oscillating and carrying out ultrasonic extraction; filtering extract liquor by using an organic filter membrane, and simultaneously transferring the filtered extract liquor into a K-D concentration bottle; adding a dewatering desiccant to the filtered extract liquor; sucking the moisture, and then putting the K-D concentration bottle into a rotary evaporator and concentrating; purging the concentrated liquid by nitrogen until the volume is smaller than 1ml; enabling the concentrated filtered extract fluid to achieve a constant volume of 1mL, and then transferring the fluid to a special agilent bottle; detecting the fluid by virtue of a combined instrument of high performance liquid chromatography-tandem three-stage mass spectrometry by adopting an internal standard method; and analyzing a chromatography-mass spectrometry analysis graph, thereby finishing detection. According to the method, the content of sarafloxacin adsorbed on the suspended particulate matters in a water environment can be detected, and the blank of antibiotics detection is filled.

A screening method for a nucleic acid aptamer specifically binding to sarafloxacin hydrochloride relates to the technical field of chemical analysis. The invention provides a nucleic acid aptamer that has higher affinity and specificity than protein antibodies, has no immunogenicity, can be chemically synthesized, has small molecular weight and stable properties and can be used for the detection of sarafloxacin hydrochloride. The invention adopts SELEX technology, takes sarafloxacin hydrochloride as a target, and screens out the nucleic acid aptamer specifically binding to sarafloxacin hydrochloride.

The present invention features an antibacterial composition comprising 1) a composition A comprising polymyxin B and trimethoprim; and 2) an antibiotic agent selected from the group consisting of rifampicin, rifabutin, rifapentine, rifaximin, pefloxacin mesylate, sparfloxacin, sarafloxacin HCl, tobramycin, lomefloxacin, besifloxacin, danofloxacin mesylate, enrofloxacin, nadifloxacin and clinafloxacin, a topical pharmaceutical thereof, and a method of treating bacterial infections using mixtures of 1 and 2.

A method for extracting and analyzing quinolone drugs by using a DPX tip-type dispersed solid-phase micro-extraction column relates to a method for detecting quinolone drug residues in animal-derivedfood. The invention aims to solve the problem that there is no effective method for detecting quinolone drugs in animal-derived food, especially measuring with quantitative high-precision, high-throughput and low-consumption in the prior art. According to the invention, a sample preparation and high performance liquid chromatography-tandem mass spectrometry method for detecting multi-residues of quinolones in animal-derived food is established. The method is suitable for the detection of single or multiple drug residues of enrofloxacin, ciprofloxacin, sarafloxacin, norfloxacin, ofloxacin, lomefloxacin and danofloxacin in animal-derived food.