Method for extracting and analyzing quinolone drugs by using DPX tip-type dispersed solid-phase micro-extraction column

A technology for dispersing solid phase and quinolones, which is applied in analytical materials, material separation, measurement devices, etc., can solve problems such as unfavorable qualitative and quantitative analysis, failure to meet detection requirements, complicated operation steps, etc., and achieve cost and time savings. The effect of sample size reduction and exposure reduction

Active Publication Date: 2018-10-23
无锡微色谱生物科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

However, some of these methods cannot meet the current detection requirements, and some of the entire operation steps are cumbersome and time-consuming, which is not conducive to large-scale qualitative and quantitative analysis.

Method used

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  • Method for extracting and analyzing quinolone drugs by using DPX tip-type dispersed solid-phase micro-extraction column
  • Method for extracting and analyzing quinolone drugs by using DPX tip-type dispersed solid-phase micro-extraction column
  • Method for extracting and analyzing quinolone drugs by using DPX tip-type dispersed solid-phase micro-extraction column

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specific Embodiment approach 1

[0033] Specific embodiment one: the method for LC-MS / MS detection of quinolone residues in animal-derived food using DPX tip-type dispersive solid-phase microextraction column of the present embodiment is carried out according to the following steps:

[0034] 1. Sample extraction: Weigh the sample, add deionized water for homogenization, add acetonitrile, vortex, then centrifuge, collect the supernatant, blow dry with nitrogen, and use 0.05-0.15% acetic acid by volume After 700-800 μL of the aqueous solution is dissolved, the purified solution is obtained;

[0035] 2. Purification: first clean the DPX Tips TM -CX tip-type dispersive solid-phase microextraction column is equilibrated with 450-550 μL of methanol and 450-550 μL of water in sequence; then pipette the liquid to be purified through the column; then use 700-800 μL of 2% formic acid aqueous solution in sequence, Wash the column with 700-800 μL of methanol; finally elute with 700-800 μL of eluent; collect the eluate, ...

specific Embodiment approach 2

[0055] Embodiment 2: This embodiment differs from Embodiment 1 in that the mass-to-volume ratio of the sample to water described in step 1 is 202-108 mg: 1 μL.

[0056] Others are the same as in the first embodiment.

specific Embodiment approach 3

[0057] Embodiment 3: This embodiment differs from Embodiment 1 in that the mass-volume ratio of the sample to acetonitrile described in Step 1 is 0.202-0.108 mg: 1 mL.

[0058] Others are the same as in the first embodiment.

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Abstract

A method for extracting and analyzing quinolone drugs by using a DPX tip-type dispersed solid-phase micro-extraction column relates to a method for detecting quinolone drug residues in animal-derivedfood. The invention aims to solve the problem that there is no effective method for detecting quinolone drugs in animal-derived food, especially measuring with quantitative high-precision, high-throughput and low-consumption in the prior art. According to the invention, a sample preparation and high performance liquid chromatography-tandem mass spectrometry method for detecting multi-residues of quinolones in animal-derived food is established. The method is suitable for the detection of single or multiple drug residues of enrofloxacin, ciprofloxacin, sarafloxacin, norfloxacin, ofloxacin, lomefloxacin and danofloxacin in animal-derived food.

Description

technical field [0001] The invention relates to a method for detecting quinolone residues in animal-derived foods. Background technique [0002] Quinolones are a class of artificially synthesized compounds containing a 4-quinone nucleus. They are a class of broad-spectrum antibiotics because of their wide antibacterial spectrum, high efficiency, low toxicity, strong tissue penetration, and low price. Features, quinolones have become one of the most important anti-infective drugs in veterinary clinical treatment and animal husbandry and aquaculture. At the same time, these drugs can promote animal daily gain and improve feed conversion rate. The domestic use of quinolones in animals It is close to 500 tons / year. But drug abuse can lead to drug residues in animal tissues, causing long-term health effects, including antibiotic resistance in microbes. At the same time, since some of these drugs are used as veterinary drugs, there are still many varieties used in human clinical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 杨佳卡莫莱费静王倩王珏
Owner 无锡微色谱生物科技有限公司
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