The invention discloses an enzyme-linked immunosorbent assay method for multi-residue analysis of a cyano-containing pyrethroid pesticide, belonging to the technical field of immunoassay. The enzyme-linked immunosorbent assay method uses a coupled complex of hapten m-phenoxy benzal cyanohydrin succinate ester and ovalbumin as a coating antigen, samples of the cyano-containing pyrethroid and a polyclonal antibody of the m-phenoxy benzal cyanohydrin succinate ester are added, then the coating antigen and the cyano-containing pyrethroid to be detected are competed and reacted with the antibody, an enzyme-labeled secondary antibody is added to be combined with the fixed antibody, washing liquid is used for washing, developer is added, an enzyme-labeled instrument is used for detecting OD value, the OD value is compared with a standard curve of a standard product of the cyano-containing pyrethroid, and the concentration of the cyano-containing pyrethroid of the samples to be detected is calculated. The enzyme-linked immunosorbent assay method can accurately and sensitively detect the residues of the cyano-containing pyrethroid pesticide in the samples to be detected, the pre-treatment process of the samples is simple with less time-consuming, a large number of samples can be simultaneously detected, and the detection cost of the samples is far lower than the detection method of the traditional instrument; furthermore, the method of the invention has good stability and no radioactive pollution.