68 results about "Multi residue" patented technology

The invention provides an UPLC-MS / MS method for analyzing four categories and 29 types of restricted veterinary drug residues in animal foodstuff. The method is characterized in that an advanced QuEChERS method is adopted for conducting sample preprocessing, a sample is subjected to McIlvaine buffering liquid-acetonitrile extraction, an extracting solution is subjected to acidification and salting out, a small amount of acetonitrile phase is taken, a C18 and NH2 adsorbing agent is utilized for conducting dispersive solid-phase extraction and purification, after purification liquid is subjected to concentration and volume setting, an ultra-high performance liquid chromatography-tandem mass spectrometer (UPLC-MS / MS) is adopted to conduct detection. The problem that it is difficult for tetracycline class veterinary drug to be extracted and purified along with other types of veterinary drugs is solved, meanwhile the sample processing steps are simplified, the UPLC-MS / MS method has the advantages of being efficient, rapid, low in cost, simple and convenient to operate, good in purification effect and high in sensitivity, and the accuracy and precision both meet the requirement of a multi-residue analysis method.

The invention discloses a device for testing burning rate of multi-residue solid propellant, a combustion chamber and a filter collector of which are connected into a whole. The combustion chamber is internally provided with a burn-out proof round bakelite sheet which is made of insulating wood material, arranged between two target lines and penetrates through 6 tapered binding posts arranged on a foundation with the view to avoiding undesirable disruption in the testing as hot combustion products come off, which affects normal operation. The top of the combustion chamber is provided with a transparent observation window, the middle part of the shell of the combustion chamber is provided with two transparent observation windows which are arranged in a 180-degree staggering manner along the circumferential direction, thus facilitating experimental observation.

The invention provides a fast high-accuracy method for detecting agricultural and veterinary products residues in food, including following four steps: (1) matrix pre-treatment; (2) adding analysis protecting agent; (3) introducing sample and detecting on machine; (4) data processing. The detecting method, under the condition that GC / MS instrument detecting sensitivity is no changed, and with large volume injection, could reduce pre-treatment sample amount sharply. At the same time the invention has the advantages of low analysis cost, short analysis time, and uses the standard solution containing analysis protecting agent as standard working solution to draw standard curve for quantifying agricultural and veterinary products residues in various kinds of vegetable, fruit and corn substrates, and quantifies agricultural and veterinary products residues in various kinds of substrates with one standard curve, and has good applicability, the sensibility, accuracy and precision are all in accordance with command of agricultural and veterinary products multi-residues technology, and is suitable for detecting various kinds of agricultural and veterinary products' residues in food quickly and sensitively.

The invention discloses a multi-residue colloidal-gold rapid detection kit and a detection method and application thereof, belonging to the field of immunology. The kit provided by the invention mainly comprises a multi-residue colloidal-gold rapid detection card; the detection card comprises a detector bar and a plastic card shell, and the detector bar is composed of a sample pad, a colloidal-gold antibody binding pad, a nitrocellulose membrane and a water absorbing pad. The colloidal-gold antibody binding pad comprises a colloidal gold labeled gentamycin monoclonal antibody, a colloidal gold labeled kanamycin monoclonal antibody and a colloidal gold labeled fluoroquinolone monoclonal antibody. The central part of the nitrocellulose membrane is coated with a detection line and a control line, wherein the detection line is arranged to be a protein conjugate and the control line is a goat anti-mouse IgG monoclonal antibody. The rapid detection kit provided by the invention can realize simultaneous detection of a plurality of residues only through simple pre-treatment, the whole operation process is simple, the kit is convenient to carry, result determination is rapid and accurate, and the kit is applicable to on-site monitoring and to qualitative screening of considerable samples.

The invention discloses an enzyme-linked immunosorbent assay method for multi-residue analysis of a cyano-containing pyrethroid pesticide, belonging to the technical field of immunoassay. The enzyme-linked immunosorbent assay method uses a coupled complex of hapten m-phenoxy benzal cyanohydrin succinate ester and ovalbumin as a coating antigen, samples of the cyano-containing pyrethroid and a polyclonal antibody of the m-phenoxy benzal cyanohydrin succinate ester are added, then the coating antigen and the cyano-containing pyrethroid to be detected are competed and reacted with the antibody, an enzyme-labeled secondary antibody is added to be combined with the fixed antibody, washing liquid is used for washing, developer is added, an enzyme-labeled instrument is used for detecting OD value, the OD value is compared with a standard curve of a standard product of the cyano-containing pyrethroid, and the concentration of the cyano-containing pyrethroid of the samples to be detected is calculated. The enzyme-linked immunosorbent assay method can accurately and sensitively detect the residues of the cyano-containing pyrethroid pesticide in the samples to be detected, the pre-treatment process of the samples is simple with less time-consuming, a large number of samples can be simultaneously detected, and the detection cost of the samples is far lower than the detection method of the traditional instrument; furthermore, the method of the invention has good stability and no radioactive pollution.

The invention discloses a diethoxy thiophosphate organophosphorus pesticide antibody and a preparation method and the application thereof. The antibody of the invention is prepared by introducing appropriate active arms to a diethoxy thiophosphate structure to prepare immunity hapten, coupling the hapten with bovine serum albumin to prepare immunity antigen and immunizing the originally immune New Zealand white rabbits; and a multi-residue ELISA detection method of the diethoxy thiophosphate organophosphorus pesticide is established with coatingen. Based on the detection method established by the antibody, more than 12 types of the diethoxy thiophosphate organophosphorus pesticide can be detected simultaneously, the limit of detection is 0.0002-3.938mug / ml and the detection method is suitable for on-site supervision of pesticide residues and rapid screening with high flux.

The invention discloses an immunochromatography assay for quantitatively detecting organophosphorus pesticides. The immunochromatography assay is characterized in that the principle combining the organophosphorus pesticides and acetylcholin esterase is combined with the immunochromatography technology, a monoclonal antibody for resisting electric eel acetylcholin esterase is prepared, the site, combining with the electric eel acetylcholin esterase, of the antibody is identical with the site, combining with the electric eel acetylcholin esterase, of organophosphorus, the monoclonal antibody is used to fix a detection line, the acetylcholin esterase is used to modify an indicator, the immunochromatography assay for quantitatively detecting the organophosphorus pesticides is provided to achieve the multi-residue detection of the organophosphorus pesticides, and the quantitative detection of the organophosphorus pesticides is achieved according to the signal strength of the detection line and a quality control line. The immunochromatography assay has the advantages that the marking of the organophosphorus pesticide indicator and the chemical preparation of protein conjugates are avoided, detection procedures are simplified, human health damage and severe environment pollution caused by the use of standard organophosphorus pesticides are reduced, and the immunochromatography assay is high in detection sensitivity and capable of satisfying the detection requirements of accuracy, quickness and field high throughput.

The invention discloses a method for detecting avermectins pesticide multi-residues in cereal agricultural products, which comprises the following steps of: extracting the avermectins pesticide residues from the cereal agricultural products with acetonitrile, separating an organic phase containing avermectins pesticides from a water phase under the action of salting-out, performing fluorescence derivatization on the extracted avermectins pesticides by a trifluoro acetic anhydride (TFAA)-N-methylimidazole (NMIM)-acetonitrile (ACN) method in an anhydrous state, and realizing the trace residue analysis of the pesticides by adopting a high performance liquid chromatography-fluorescence detection method. The method of the invention has the advantages of performing trace analysis and detection on the residues of avermectin, emamectin benzoate and ivermectin in a plurality of cereal agricultural products, such as rice, wheat, corn and the like, along with simplicity, convenience, time saving, low cost and the like.

The invention discloses a preparation method of a multi-template molecular imprinting integrated bar, and belongs to the technical fields of preparation of polymer materials and pretreatment of samples. The method comprises the following steps of: getting a hard glass capillary as a reaction vessel of a molecular imprinting polymer integrated bar; cleaning the capillary; drying; sintering one end; adding a certain amount of molecular imprinting pre-assembling solution; heating with water bath to initiate polymerization; removing the capillary and ageing after polymerization; capturing with a proper length; eluting template molecule to obtain multi-template molecular imprinting integrated bar for extracting and analyzing the samples. The prepared multi-template molecular imprinting integrated bar is able to selectively separate and enrich sulfanilamide antibiotics and antibacterial synergist compounds at the same time, and is applicable to multi-residue synchronous analysis of the sulfanilamide antibiotics and antibacterial synergists in the food and environmental samples.

The invention discloses a single-chain antibody and application thereof in detecting aflatoxin. The single-chain antibody is a polypeptide consisting of a light chain variable region, a connection peptide and a heavy chain variable region, wherein the connection peptide is positioned between the light chain variable region and the heavy chain variable region; the light chain variable region is represented by amino acid residues from 1st to 110th site of the tail ends of sequences from 1 to N of a sequence table; and the heavy chain variable region is represented by amino acid residues from 131st to 248th site of the tail ends of the sequences from 1 to N of the sequence table. The single-chain antibody has high cross reactivity, high affinity and high sensitivity to various types of aflatoxin, is suitable for quick immune detection for various types of multi-residue aflatoxin and has great significance for detecting the aflatoxin.

The invention relates to an enzyme-linked immunodetection method of aminoglycoside antibiotics in animal-derived foods, belonging to the immnunoassay field. The invention utilizes a carbodiimide method to synthesize immunogen (NM)L-(KM)m-(GM)n-(SM)k-BSA with multiple antigen determinants, such as aminoglycoside antibiotics, by four steps, utilizes a glutaric dialdehyde method to synthesize the envelope antigen of the aminoglycoside antibiotics and establish the enzyme-linked immunodetection method of the aminoglycoside antibiotics in the animal-derived foods; a polyclonal antibody with the specificity of aminoglycoside cluster is adopted as a detection reagent; gentamicin, neomycin, kanamycin or streptomycin is adopted as a standard substance, the four antibiotics and OVA conjugate are adopted as the envelope antigens, and the establishment of an ELISA method for detecting multi-residue of the aminoglycoside antibiotics provides a detecting means with high speed and high efficiency for the multi-residue detection of the aminoglycoside antibiotics in the animal-derived foods. The invention has the advantages of simple pre-processing, high sensitivity, high precision and the like.

The invention provides a gas chromatography agriculture residue analysis protecting agent, which includes acetone solution containing polyethylene glycol and olive oil, wherein the concentration of polyethylene glycol is 0.5-2.0g / 100mL, the concentration of olive oil is 21.25-63.75g / 100mL. Wherein the concentration of polyethylene glycol is preferably 1.0g / 100mL, the concentration of olive oil is preferably 42.5g / 100mL; the provided gas chromatography agriculture residue analysis protecting agent has simple preparation, and has good solubility in acetone solution which overcomes bad eater solubility of other protecting agents, and avoids to bring water into gas chromatography (GC) system when introducing sample, which makes bad effect for system, especially for chromatography column. At the same time the product can improve the peak form and intensity of the detecting material and reduce detectability well for realizing multi-residue high-sensitivity analysis.

The invention relates to a method for quinoxalone antibiotics multi-residue enzyme-linked immune detection, and belongs to the technology field of the antibiotics residue detection method. In the method, the polyclonal antibodies are obtained by utilizing syntactic quinoxalone antibiotics multi-cluster antigen immunization; the coupling compound of the half antigen of the quinoxalone antibiotics and an egg-white protein OVA is taken as an envelope antigen; the quinoxalone antibiotics is taken as a standard; the indirect competitive enzyme-linked immune detection method for the quinoxalone antibiotics in animal food is established; a rapid and high efficient detection means is provided for the residues of the quinoxalone antibiotics in the animal food; because the polyclonal antibodies are adopted, the cost is low, and the stability and the repeatability are good; the detection limit (IC is smaller than 90) is 0.0126 ng / ml, the median inhibitory dose (IC is smaller than 50) is 0.52 ng / ml, and the detection range (IC is smaller than 20 to 80) is 0.04467 to 14 ng / ml. In addition, the ELISA method is also evaluated in the aspects of specificity, accuracy and sensitivity, thereby conforming to the detection requirements for the multi-residue ELISA method.

The invention provides a method for preparing an immunity chromatographic test strip for testing cephalosporins antibiotics, and relates to the immunity testing field. The test strip is composed of a sample pad, a combined pad, a cellulose nitrate membrane, a water absorbing pad and a PVC backing; the sample pad, the combined pad, the cellulose nitrate membrane and the water absorbing pad are sequentially stuck to the PVC backing; the combined pad is coated with a group-specific anti-cephalosporins antibiotic antibody colloidal gold label of anti-cephalosporins antibiotics; and the cellulose nitrate membrane is sequentially coated with a cefalexin-OVA test line and a goat anti-rabbit IgG quality control line. The method prepares the test trip by assembling the test preparation of the combination of the group-specific antibody of the anti-cephalosporins antibiotics and the chromogenic colloidal gold, and carries out a multi-residue screening test of the cephalosporins antibiotics with low cost and rapidness; and the portable test strip which can be used for the cephalosporins antibiotic residue tests of different samples is the application of the immunity chromatographic analysis method.

The invention relates to a multi-cluster antigen and an antibody of beta-adrenoceptor agonists and application of the multi-cluster antigen and the antibody to detection on multi-residue of the beta-adrenoceptor agonists. The application is implemented by a detection kit and test paper. The multi-cluster antigen of the beta-agonists is salbutamol-ractopamine-bovine serum albumin (SAL-RAC-BSA) or salbutamol-ractopamine-ovalbumin (SAL-RAC-OVA), and the salbutamol-ractopamine-bovine serum albumin antibody (SAL-RAC-BSAAb) is prepared from SAL-RAC-BSA through animal immunization. The invention also discloses the kit and the test paper, which are used for detecting the multi-residue of the beta-agonists and take the SAL-RAC-BSAAb as a core agent. Based on a cross immunization reaction of the SAL-RAC-BSAAb on the beta-agonists, three beta-agonists, namely clenbuterol, SAL and RAC, can be detected.

The invention discloses a fluorescein marker and an organic phosphorus pesticide homogeneous phase multi-residue immune detection method based on fluorescence polarization. In the invention, the fluorescence polarization value to be detected can be generated by mixing a sample to be detected, a fluorescence tracer and an organic phosphorus pesticide multi-specificity antibody and combining the fluorescence tracer and the organic phosphorus pesticide multi-specificity antibody, the fluorescence polarization value of the mixture is measured and a standard curve is established through a series of organic phosphorus pesticide standards with known concentrations, and the concentration of the organic phosphorus pesticide in the sample to be detected is calculated by utilizing the standard curve. The method disclosed by the invention has the advantages of easy pre-treatment of the sample, no need for washing separation in the reaction process, detection time of less than 3-5min, convenience for operation, capability of simultaneously detecting 20 organic phosphorus pesticides, sensitivity between 0.003-1.0mg / kg, and capability of simultaneously detecting 42 samples by using 96 micro-plates (each sample is paralleled twice), and is a fast, simple and convenient organic phosphorus pesticide high flux detection method with high sensitivity.

The invention discloses an enzyme-linked immunoassay kit for detecting quinolone drugs. The enzyme-linked immunoassay kit for detecting quinolone drugs provided in the invention includes an enzyme-labelled plate coated with a quinolone drug-carrier protein conjugate, an antibody working solution, an enzyme labeled secondary antibody, quinolone series standard substances, a concentrated complex solution, a concentrated washing solution, a substrate color-developing solution, and a stopping solution. The enzyme-linked immunoassay kit for detecting quinolone drugs is a multi-residue detection kit, and can simultaneously determine the total residue of 12 quinolone drugs in aquatic product (fish, shrimp) samples. The invention also discloses a method for detecting quinolone drugs by the enzyme-linked immunoassay kit. The method comprises: first conducting sample pretreatment, then carrying out detection with the kit, and finally analyzing the detection result. The kit disclosed in the invention has the advantages of simple operation, low cost, high sensitivity, and a total operation time of only 45 minutes, can perform on-site monitoring and is suitable for screening of a large number of samples.

A method for extracting and analyzing quinolone drugs by using a DPX tip-type dispersed solid-phase micro-extraction column relates to a method for detecting quinolone drug residues in animal-derivedfood. The invention aims to solve the problem that there is no effective method for detecting quinolone drugs in animal-derived food, especially measuring with quantitative high-precision, high-throughput and low-consumption in the prior art. According to the invention, a sample preparation and high performance liquid chromatography-tandem mass spectrometry method for detecting multi-residues of quinolones in animal-derived food is established. The method is suitable for the detection of single or multiple drug residues of enrofloxacin, ciprofloxacin, sarafloxacin, norfloxacin, ofloxacin, lomefloxacin and danofloxacin in animal-derived food.

The invention discloses a single-chain antibody and application thereof in detecting a beta-stimulant. The single-chain antibody is a polypeptide consisting of a light chain variable region, a connection peptide and a heavy chain variable region, wherein the connection peptide is positioned between the light chain variable region and the heavy chain variable region; the light chain variable region is represented by amino acid residues from 1st to 122nd site of the tail ends of sequences from 1 to N of a sequence table; and the heavy chain variable region is represented by amino acid residues from 138th to 246th site of the tail ends of the sequences from 1 to N of the sequence table. The single-chain antibody has high cross reactivity, high affinity and high sensitivity to various types of beta-stimulants, is suitable for quick immune detection for various types of multi-residue beta-stimulants and has great significance for detecting the beta-stimulant.

Disclosed is a method of quantum dot-labeled indirect competitive fluorescence immunoassay of glucocorticoid multi-residue, which belongs to the immunoassay method technique field. Quantum dots for labeling antibodies of the invention have the emission spectra of QD545, QD590 and QD650, and the method comprises: directly covering coating antigens in micro-holes of an enzyme label plate, adding glucocorticoid standard solution or a sample under test to form an antigen-antibody fluorescence immunity compound body, stimulating and detecting the fluorescence intensity of the formed antigen-antibody fluorescence immunity compound body with a fluorescence enzyme-labeling instrument, and obtaining the concentration of glucocorticoid multi-residue in the sample under test through comparing with the standard solution. The invention can indirectly detect the concentration value of glucocorticoid multi-residue in the sample under test without adding chromogenic substance through the fluorescence intensity of the antigen-antibody immunity compound body, and both the operation and reaction need only one step; and the quantum dots for labeling antibodies of the invention have advantages of stronger emitted fluorescence intensity and long stabilization time of fluorescence compared with the traditional fluorescence.

The invention discloses a veterinary drug multi-residue (ractopamine, beta2-stimulant, chloramphenicol, florfenicol and metabolin florfenicol amine) immune detection system which comprises an immune chip for detecting veterinary drug multi residues, an image collecting device and mobile equipment provided with a detection APP, wherein the detection APP can collect an immune chip color developing image and read an image gray value, and operation of the detection APP to collect the immune chip color developing image is finished in the image collecting device; a standard curve between the immunechip color developing image gray value and a veterinary drug residue standard content is set in the detection APP. In detection, the immune chip is utilized to detect a target article to be detected;after immune chip color developing is stable, the image collecting device is utilized, and the detection APP is utilized to collect an immune chip color developing image reading gray valve; thus, a content of the article to be detected is analyzed out, a detection result is directly displayed on a screen of the mobile equipment, and the purpose of quickly detecting varieties of veterinary drug residues in site is achieved.

The invention discloses a broad spectrum specific molecularly imprinted polymer of quinolone medicine, a chemiluminescence kit using the broad spectrum specific molecularly imprinted polymer as a recognition element, a detection method and application. The molecularly imprinted polymer uses pipemidic acid as a pseudomolecular template. The synthesized broad spectrum specific molecularly imprintedpolymer can recognize eight kinds of quinolone medicine. The chemiluminescence kit prepared on the basis of the molecularly imprinted polymer can be used for performing multi-residue detection on eight kinds of quinolone medicine in meat tissues; the repeated utilization can be realized; the detection sensitivity is improved; the detection time is shortened; the detection cost is reduced.

The invention relates to a preparation and using method of a reagent of multi-residue simultaneous rapid fluorescence detection of aminoglycoside antibiotics. Three kinds of quantum dots with the samematerial core and different emission wavelengths are coupled with an aptamer having the high specific recognition ability for kanamycin, netilmicin and tobramycin; the three kinds of coupling mattersand graphene oxide to form a fluorescence resonance energy transfer system; and a reagent capable of realizing simultaneous rapid fluorescence detection of kanamycin, netilmicin and tobramycin residues is prepared. With the provided reagent, rapid, accurate and sensitive detection of kanamycin, netilmicin and tobramycin residues in milk samples can be realized without depending on the separationanalysis technology; and the powerful technical guarantee is provided for the g food safety supervision enhancement.

The invention provides a detecting method of a pesticide multi-residue in fresh tea leaves. The method comprises the following steps that firstly, a fresh tea leaf sample which is homogenated is placed in a centrifuge tube, and after acetonitrile is added, vortex is carried out; secondly, anhydrous magnesium sulfate and sodium chloride are carried out, and the vortex is carried out; thirdly, centrifuging is carried out; fourthly, centrifuged supernatant liquor is obtained to be subjected to purifying; fifthly, detecting is carried out by adopting the gas chromatography- tandem mass spectrometry method. According to the method, the tea leaves are purified by a PSA / GCB solid phase extraction column, the pesticide residue in the tea leaves are detected by adopting the gas chromatography- tandem mass spectrometry method at the same time, the method is rapid, accurate, high in sensitivity, and suitable for multi-residue screening and detecting in the fresh tea leaves.

The invention discloses a preparation method of a nitrosamine sterilization byproduct molecular marker polymer and application. The preparation method comprises the following steps: pre-polymerizing afunctional monomer by using a template molecule, enabling the obtained pre-polymer system and ethylene glycol metacrylic acid ester to react with azodiisobutyronitrile so as to obtain a high-molecular polymer, and performing physical and chemical cleaning on the high-molecular polymer, thereby finally obtaining a nitrosamine sterilization byproduct molecularly imprinted polymer. The obtained molecularly imprinted polymer is regular in morphology, five nitrosamine substances can be adsorbed simultaneously, rapid separation, enrichment and multi-residue detection on the nitrosamine substances in complex water samples are achieved, and good application prospects are met.

The invention discloses a method for simultaneous SMART column on-line purification and HPLC / UVE fluorescent detection of four aflatoxins in a peanut product. The method comprises the following steps: (1) sample treatment; and (2) SMART immunoaffinity column on-line purification and HPLC / UVE fluorescent detection: loading a SMART immunoaffinity column with liquid extract, carrying out leaching, drying and elution, allowing eluate to directly enter a HPLC chromatographic column, treating liquid having passed through the chromatographic column with a post-UVE column derivatization reactor, then detecting the treated liquid with a fluorescence detector, drafting a standard curve and quantifying the four aflatoxins by using an external standard method according to peak areas. The method is simple in pre-treatment, good in purifying effect, high in sensitivity, good in repeatability and suitable for multi-residue rapid detection of trace aflatoxins in peanut.

Relating to the technical field of analysis and detection, the invention discloses preparation of a chitosan nanoparticle and application thereof in pesticide residue detection. The preparation method of the chitosan nanoparticle comprises the steps of: S1.1: blending 1-2 parts of chitosan into 120-140 parts of plasma water, then adding a glacial acetic acid solution, and making the concentration of glacial acetic acid at 1%-1.4%; S1.2: stirring the solution in step S1 till complete dissolution; S1.3: performing ultrasonic oscillation for 3-8min, conducting centrifugation for 20-30min, discarding the supernate, and conducting freezing and drying. The preparation method is simple, simplifies the preparation process of chitosan nanoparticle, lowers the production cost, and the produced chitosan nanoparticle has good stability, the chitosan nanoparticle is employed for pesticide residue detection, improves the detection precision, brings great convenience to detection personnel, and realizes on-line rapid detection of pesticide multi-residue.

The invention belongs to the field of bio-engineering, and particularly relates to a method for preparing recombinant porcine beta 2-adrenergic receptor protein and an application of the recombinant porcine beta 2-adrenergic receptor protein in rapidly detecting beta 2 agonist medicaments. The recombinant porcine beta 2-adrenergic receptor protein obtained by separation and purification has beta 2 agonist binding activity, SDS-PAGE electrophoresis shows that the molecular weight of the recombinant protein is about 48kDa and the purity of the recombinant protein is more than 80%. The recombinant protein can specifically adsorb beta 2 agonist medicaments and three beta 2 agonist medicaments standard products with different concentrations have an inhibition effect on the reaction when detection is carried out by a competitive ELISA method. According to method disclosed by the invention, the beta 2AR receptor capable of specifically binding with beta 2 agonist is efficiently expressed by a molecular biology method and can be used for rapidly screening beta 2 agonist medicaments instead of conventional antibodies to achieve the detection of multi-residue and screening of unknown substance in medicaments, and the method has good application prospects.

The invention relates to a method for detecting multi-residues of antibiotics by a magnetic nano fluorescence sensor based on an aptamer, belonging to the field of food safety analysis. According to the method, terramycin and kanamycin aptamers are simultaneously modified on the surface of a nano magnetic micro-sphere; complementary chains of fluorescence radical marks are respectively combined by base complementation; when an object to be detected exists, the aptamer is combined with the object to be detected to cause complementary chain dissociation to change fluorescence intensity, so that the aim of quantitatively detecting terramycin and kanamycin is realized. The method utilizes a magnetic nano material to rapidly separate the target to be detected in a sample so that the detection time is shortened, the detection cost is reduced, the detection sensitivity is improved, the operation is simple and the aim of analyzing the multi-residues is realized.