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Preparation of chitosan nanoparticle and application thereof in pesticide residue detection

A pesticide residue detection and chitosan nanotechnology, which is applied in the field of analysis and detection, can solve the problems of complicated pesticide detection operation steps, complex preparation methods, low detection accuracy, etc., to achieve online rapid detection, simplify preparation process, and improve detection accuracy. Effect

Inactive Publication Date: 2017-11-17
桐城师范高等专科学校
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a kind of preparation of chitosan nanoparticle and its application in pesticide residue detection, to solve the complex preparation method of existing chitosan nanoparticle proposed in the above-mentioned background technology, the cost is too high, Moreover, the operation steps of pesticide detection are cumbersome and the detection accuracy is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation method of chitosan nanoparticles includes the following steps:

[0021] S1.1: Mix 1 part of chitosan into 120 parts of plasma water, and then add glacial acetic acid solution to make the concentration of glacial acetic acid 1%;

[0022] S1.2: Stir the solution in step S1 until it is fully dissolved, the stirring rate is 250 rpm, and the stirring time is 10 hours;

[0023] S1.3: Use ultrasonic vibration for 3 minutes, ultrasonic power of 80W, centrifugation for 20 minutes, centrifugal speed of 11000 rpm, discard the supernatant, freeze and dry.

Embodiment 2

[0025] The preparation method of chitosan nanoparticles includes the following steps:

[0026] S1.1: Mix 2 parts of chitosan into 140 parts of plasma water, and then add glacial acetic acid solution to make the concentration of glacial acetic acid 1.4%;

[0027] S1.2: Stir the solution in step S1 until it is fully dissolved, the stirring rate is 280 rpm, and the stirring time is 14 hours;

[0028] S1.3: Use ultrasonic vibration for 8 minutes, ultrasonic power of 140W, centrifugation for 30 minutes, centrifugal speed of 15000 rpm, discard the supernatant, freeze and dry.

Embodiment 3

[0030] The preparation method of chitosan nanoparticles includes the following steps:

[0031] S1.1: Mix 1.5 parts of chitosan into 130 parts of plasma water, and then add glacial acetic acid solution to make the concentration of glacial acetic acid 1.2%;

[0032] S1.2: Stir the solution in step S1 until it is fully dissolved, the stirring rate is 265 rpm, and the stirring time is 12 hours;

[0033] S1.3: Use ultrasonic vibration for 5 minutes, ultrasonic power of 110W, centrifugation for 25 minutes, centrifugal speed of 13000 rpm, discard the supernatant, freeze and dry.

[0034] The present invention also provides an application method of chitosan nanoparticles in pesticide residue detection. The application method of chitosan nanoparticles in pesticide residue detection includes the following steps:

[0035] S2.1: Take 3 parts of agricultural products, add 120 parts of deionized water, pulverize, add acetonitrile-acetone solution, centrifuge, and extract;

[0036] S2.2: Use a stir bar...

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PUM

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Abstract

Relating to the technical field of analysis and detection, the invention discloses preparation of a chitosan nanoparticle and application thereof in pesticide residue detection. The preparation method of the chitosan nanoparticle comprises the steps of: S1.1: blending 1-2 parts of chitosan into 120-140 parts of plasma water, then adding a glacial acetic acid solution, and making the concentration of glacial acetic acid at 1%-1.4%; S1.2: stirring the solution in step S1 till complete dissolution; S1.3: performing ultrasonic oscillation for 3-8min, conducting centrifugation for 20-30min, discarding the supernate, and conducting freezing and drying. The preparation method is simple, simplifies the preparation process of chitosan nanoparticle, lowers the production cost, and the produced chitosan nanoparticle has good stability, the chitosan nanoparticle is employed for pesticide residue detection, improves the detection precision, brings great convenience to detection personnel, and realizes on-line rapid detection of pesticide multi-residue.

Description

Technical field [0001] The invention relates to the technical field of analysis and detection, in particular to the preparation of chitosan nano particles and its application in pesticide residue detection. Background technique [0002] Chitosan, also known as chitin, is obtained by deacetylation of chitin, which is widely present in nature, and its chemical name is polyglucosamine (1-4)-2-amino-B-D glucose. Since the French Rouget first obtained chitosan in 1859, the excellent properties of this natural polymer, such as biofunctionality and compatibility, blood compatibility, safety, and microbial degradability, have attracted wide attention from all walks of life. Significant progress has been made in applied research in many fields such as medicine, food, chemicals, cosmetics, water treatment, metal extraction and recovery, biochemical and biomedical engineering. The research results show that when the particle size of chitosan becomes smaller, the effect of chitosan is bette...

Claims

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Application Information

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IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 王宏伟周琳方皓文王斌汪若冰
Owner 桐城师范高等专科学校
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