Aminoside antibiotics ELISA detection method in Animal derived food

Abstract

The invention relates to an enzyme-linked immunodetection method of aminoglycoside antibiotics in animal-derived foods, belonging to the immnunoassay field. The invention utilizes a carbodiimide method to synthesize immunogen (NM)L-(KM)m-(GM)n-(SM)k-BSA with multiple antigen determinants, such as aminoglycoside antibiotics, by four steps, utilizes a glutaric dialdehyde method to synthesize the envelope antigen of the aminoglycoside antibiotics and establish the enzyme-linked immunodetection method of the aminoglycoside antibiotics in the animal-derived foods; a polyclonal antibody with the specificity of aminoglycoside cluster is adopted as a detection reagent; gentamicin, neomycin, kanamycin or streptomycin is adopted as a standard substance, the four antibiotics and OVA conjugate are adopted as the envelope antigens, and the establishment of an ELISA method for detecting multi-residue of the aminoglycoside antibiotics provides a detecting means with high speed and high efficiency for the multi-residue detection of the aminoglycoside antibiotics in the animal-derived foods. The invention has the advantages of simple pre-processing, high sensitivity, high precision and the like.

Description

technical field [0001] The invention relates to an enzyme-linked immunoassay method for quantitatively detecting the content of aminoglycoside antibiotics in animal source food, and belongs to the technical field of immune analysis. Background technique [0002] Aminoglycoside antibiotics (aminoglycoside antibiotics, AGs) are a class of widely used, mainly including gentamicin, neomycin, kanamycin, streptomycin, etc., the basic structure of these antibiotics are aminocyclitol and amino sugar The condensed glycosides are alkaline and have high polarity and water solubility due to the structure containing multiple amino groups and hydroxyl groups. This type of antibiotic is widely used and is a broad-spectrum antibiotic. Its function is characterized by the ability to bind to the ribosomal 30S subunit, causing it to synthesize abnormal proteins, hindering the release of easily synthesized proteins, thereby inhibiting the growth of bacteria. Clinically, aminoglycoside antibio...

AI Technical Summary

Problems solved by technology

However, due to its improper use method or non-compliance with the withdrawal period regulations, excessive or illegal addition, etc., it causes its residues in animal products and brings serious harm to human health.

Existing microbial detection methods and instrumental detection methods are difficult to achieve rapid, accurate and multi-residue detection of aminoglycoside antibiotics

At present, there are no reports on ideal multi-residue immunoassay methods for aminoglycoside drugs at home and abroad

Images