Aminoside antibiotics ELISA detection method in Animal derived food

An enzyme-linked immunosorbent assay and aminoglycoside technology, applied in the field of immunoassay, can solve problems such as residues, human health hazards, improper use methods, etc., and achieve the effects of high accuracy, high sensitivity, and simple pretreatment.

Inactive Publication Date: 2009-04-01
JIANGNAN UNIV
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Problems solved by technology

However, due to its improper use method or non-compliance with the withdrawal period regulations, excessive or illegal addition, etc., it causes its residues in animal products and brings serious harm to human health.
Existing microbial detection methods

Method used

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  • Aminoside antibiotics ELISA detection method in Animal derived food

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Embodiment Construction

[0049] The present invention will be further illustrated below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention, and are not intended to limit the scope of the present invention.

[0050] The preparation of a kit for quantitatively detecting the content of aminoglycoside antibiotics in food of animal origin comprises the following steps:

[0051]1. Preparation of immunogens with multiple epitope aminoglycoside antibiotics, see Chinese patent application number 200810123016.6 for details. The patent synthesized artificial antigen (NM) L -(KM) m -(GM) n -BSA, the present invention further synthesizes immunogen (NM) on this basis L -(KM) m -(GM) n -(SM) k -BSA.

[0052] (1). Conjugates (NM) L - Preparation of BSA

[0053] Dissolve 20-50mg of neomycin NM and 40mg of bovine serum albumin BSA in 3mL of 0.01mol / L PBS buffer, add dropwise the methanol solution of water-soluble carbodiimide EDC...

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Abstract

The invention relates to an enzyme-linked immunodetection method of aminoglycoside antibiotics in animal-derived foods, belonging to the immnunoassay field. The invention utilizes a carbodiimide method to synthesize immunogen (NM)L-(KM)m-(GM)n-(SM)k-BSA with multiple antigen determinants, such as aminoglycoside antibiotics, by four steps, utilizes a glutaric dialdehyde method to synthesize the envelope antigen of the aminoglycoside antibiotics and establish the enzyme-linked immunodetection method of the aminoglycoside antibiotics in the animal-derived foods; a polyclonal antibody with the specificity of aminoglycoside cluster is adopted as a detection reagent; gentamicin, neomycin, kanamycin or streptomycin is adopted as a standard substance, the four antibiotics and OVA conjugate are adopted as the envelope antigens, and the establishment of an ELISA method for detecting multi-residue of the aminoglycoside antibiotics provides a detecting means with high speed and high efficiency for the multi-residue detection of the aminoglycoside antibiotics in the animal-derived foods. The invention has the advantages of simple pre-processing, high sensitivity, high precision and the like.

Description

technical field [0001] The invention relates to an enzyme-linked immunoassay method for quantitatively detecting the content of aminoglycoside antibiotics in animal source food, and belongs to the technical field of immune analysis. Background technique [0002] Aminoglycoside antibiotics (aminoglycoside antibiotics, AGs) are a class of widely used, mainly including gentamicin, neomycin, kanamycin, streptomycin, etc., the basic structure of these antibiotics are aminocyclitol and amino sugar The condensed glycosides are alkaline and have high polarity and water solubility due to the structure containing multiple amino groups and hydroxyl groups. This type of antibiotic is widely used and is a broad-spectrum antibiotic. Its function is characterized by the ability to bind to the ribosomal 30S subunit, causing it to synthesize abnormal proteins, hindering the release of easily synthesized proteins, thereby inhibiting the growth of bacteria. Clinically, aminoglycoside antibio...

Claims

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Application Information

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IPC IPC(8): G01N33/543
Inventor 胥传来马伟彭池方徐丽广李灼坤
Owner JIANGNAN UNIV
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