The invention relates to the field of
gene detection, in particular to a kit and a primer pair group for detecting
drug-induced deafness
susceptibility gene mutation sites, wherein the kit and the primer pair group can be used for detecting
aminoglycoside drug susceptibility genes in mitochondria. A specific primer amplification and real-time
fluorescence PCR technology is adopted, a new primer pair group is designed aiming at
mutant nucleic acid to be detected, the primer is extended to product a specific PCR product when the
mutant nucleic acid exists, a fluorescent substance is added into each
reaction system, the fluorescent substance is bonded to the PCR product after amplification is completed, the produced melting peak is analyzed by adopting a melting curve, whether a corresponding
mutant type exists on the
nucleic acid or not is judged through the existence of the specific melting peak, and the specificity is high; the detection of two
mutation sites can be completed by using a
single tube and a single channel only, the requirements on instruments are low and the operation is simple to perform.