Pretreatment method for detecting aminoglycoside antibiotic residues in milk

A technology of aminoglycosides and antibiotics, applied in the field of analytical chemistry, can solve the problems of lack of target molecule selectivity, time-consuming, etc., and achieve the effects of rapid extraction and enrichment, overcoming weak selectivity, and uniform pore size distribution

Inactive Publication Date: 2017-12-15
FUDAN UNIV
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Problems solved by technology

[0006] The purpose of the present invention is to provide a new pretreatment method for detecting residual aminoglycoside antibiotics in milk in order to solve the defects of time-consuming, large-scale use of organic solvents, and lack o

Method used

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  • Pretreatment method for detecting aminoglycoside antibiotic residues in milk
  • Pretreatment method for detecting aminoglycoside antibiotic residues in milk
  • Pretreatment method for detecting aminoglycoside antibiotic residues in milk

Examples

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Embodiment 1

[0027] Example 1. Using boric acid to modify mesoporous magnetic graphene composite material is applied to streptomycin and dihydrostreptomycin in enriched milk:

[0028] (1) Synthesis of boric acid-modified mesoporous magnetic graphene composites:

[0029] 1) Disperse 0.30 g graphene flakes in 120 mL concentrated nitric acid, 60 o C under stirring for 7 hours, the treated graphene was cleaned with deionized water until the pH was neutral, 50 o Collect after C vacuum drying;

[0030] 2) Add 150 mg acidified graphene, 1.8 g anhydrous sodium acetate, 1.0 g PEG-20000, 150 mg trisodium citrate, 150 mg ferric chloride hexahydrate, 40 mL ethylene dichloride into a 100 mL reaction kettle Alcohol, sonicated for 2 hours, reacted at 200 °C for 12 hours, washed 5 times with absolute ethanol, 50 o Collect after C vacuum drying;

[0031] 3) Add 75 mg magnetic graphene, 750 mg cetyltrimethylammonium bromide, 75 mL deionized water to a 1 L round-bottomed flask, sonicate for 30 min, then ...

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Abstract

The invention belongs to the field of analytical chemistry and relates to a pretreatment method for detecting aminoglycoside antibiotic residues in milk. The method comprises the following steps: synthesizing a boric acid modified mesoporous magnetic graphene composite material, carrying out two-step post synthesis grafting reactions, carrying out a reaction between APB and GLYMO to obtain GLYMO-APB, and grafting GLYMO-APB onto the surface of the mesoporous magnetic graphene composite material to obtain the boric acid modified mesoporous magnetic graphene composite material. A boric acid functional group modified on the mesoporous inner wall of the composite material can be selectively combined with an aminoglycoside drug in milk through interaction between boric acid and vicinal diol structure of glycoside micromolecules, and interference of biomacromolecules such as fat, protein and the like in a milk matrix is eliminated. According to the method, antibiotic residues in milk are enriched by the utilization of specific boric acid-aminoglycoside effect, and problems such as high time consumption, poor selectivity, severe matrix interference and the like are overcome.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a pretreatment method for detecting residual aminoglycoside antibiotics in milk, in particular to a pretreatment method for enriching and detecting aminoglycoside antibiotics in milk based on boric acid-modified mesoporous magnetic graphene composite materials. The method uses boric acid as an adsorbent of functional groups to quickly eliminate endogenous interference in milk, a complex biological sample, and selectively enrich aminoglycoside antibiotics. Background technique [0002] The prior art discloses that aminoglycoside antibiotics (Aminoglycosides) are glycoside antibiotics formed by linking aminosugar and aminocycloalcohol through oxygen bridges. Such antibiotics are widely used in the breeding industry and animal husbandry to prevent and treat a series of infections such as cholera, typhoid fever, and colibacillosis in livestock and poultry. However, the residues of ...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/08
Inventor 李嫣冯嘉楠佘晓健何心莹
Owner FUDAN UNIV
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