Method for detecting aminoglycoside drug residues in animal-derived food

Abstract

The invention discloses a method for detecting aminoglycoside drug residues in animal-derived food. The method comprises the following steps: (1) preparing an aminoglycoside standard solution, and selecting a matrix for standard preparation; and (2) extracting and purifying a sample to be detected, and detecting the sample to be detected by adopting liquid chromatography-tandem mass spectrometry to obtain the residual quantity of the aminoglycoside drugs in the animal-derived food. According to the method for detecting aminoglycoside drug residues in animal-derived food, an ionization reagentis not used, aminoglycoside drug detection of multiple matrixes can be met at the same time, and the problems of pollution and ion inhibition of heptafluorobutyric acid ions to reagents and instruments can be well avoided.

Description

technical field [0001] The invention relates to a method for detecting residues of aminoglycoside drugs, in particular to a method for detecting residues of aminoglycoside drugs in animal-derived foods. Background technique [0002] Aminoglycoside veterinary drugs play an important role in the prevention and control of animal diseases, but their residues in animal-derived foods may cause serious harm to human health, and thus become a public health issue of increasing concern. The Ministry of Agriculture of my country stipulates that the maximum residue limit (MRL) of streptomycin in milk is 200 μg / kg; Japan’s positive list system stipulates that the MRL of the total amount of streptomycin and dihydrostreptomycin in beef and pork is 0.6 mg / kg; The MRLs for streptomycin in bee products, milk, meat and liver are 0.2, 0.2, 0.5 and 0.5 mg / kg respectively; the U.S. Food and Drug Administration (FDA) stipulates that the MRL for streptomycin in milk is 0.125 mg / kg ; The World Health...

AI Technical Summary

Problems solved by technology

However, because aminoglycosides are easily soluble in water and have strong polarity, their retention on the C18 column is very weak and they have no UV absorption. Currently, the most commonly used method is to enhance retention by adding heptafluorobutyric acid ion-pairing reagents. Animal-derived food matrices Complex, which brings great difficulty to the qualitative and quantitative detection of the target

The pretreatment steps of the method reported in the literature are cumbersome, the entire detection cycle is long, the matrix effect is serious, and it is difficult to obtain good peak shape and separation.

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