Method for detecting aminoglycoside drug residues in animal-derived food

An aminoglycoside, animal-derived technology, applied in the field of detection of aminoglycoside drug residues in animal-derived food, can solve the problems of difficulty in obtaining peak shape and separation, weak retention, and complicated processing steps, and avoid Contamination and ion suppression, enhanced ionization and response, reduced matrix interference

Inactive Publication Date: 2020-04-07
广电计量检测(西安)有限公司 +1
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Problems solved by technology

However, because aminoglycosides are easily soluble in water and have strong polarity, their retention on the C18 column is very weak and they have no UV absorption. Currently, the most commonly used method is to enhance retention by adding heptafluorobutyric acid ion-pairing reagents. Animal-derived food matr

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  • Method for detecting aminoglycoside drug residues in animal-derived food
  • Method for detecting aminoglycoside drug residues in animal-derived food
  • Method for detecting aminoglycoside drug residues in animal-derived food

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Embodiment Construction

[0028] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0029] This embodiment has specifically described the detection process of the detection method of the present application as follows:

[0030] (1) Standard preparation

[0031] The mobile phase standard matching and matrix standard matching were compared respectively, and representative matrices such as livestock and poultry muscle, liver, aquatic products, milk, honey, etc. were selected to study the degree of interference of different matrix effects on the detection of target objects.

[0032] Prepare standard solutions:

[0033] 1. Accurately weigh the appropriate amount of 10 kinds of aminoglycoside standard substances, each of which is prepared with water to make a standard stock solution with a concentration of 1 mg / mL, and store in a...

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Abstract

The invention discloses a method for detecting aminoglycoside drug residues in animal-derived food. The method comprises the following steps: (1) preparing an aminoglycoside standard solution, and selecting a matrix for standard preparation; and (2) extracting and purifying a sample to be detected, and detecting the sample to be detected by adopting liquid chromatography-tandem mass spectrometry to obtain the residual quantity of the aminoglycoside drugs in the animal-derived food. According to the method for detecting aminoglycoside drug residues in animal-derived food, an ionization reagentis not used, aminoglycoside drug detection of multiple matrixes can be met at the same time, and the problems of pollution and ion inhibition of heptafluorobutyric acid ions to reagents and instruments can be well avoided.

Description

technical field [0001] The invention relates to a method for detecting residues of aminoglycoside drugs, in particular to a method for detecting residues of aminoglycoside drugs in animal-derived foods. Background technique [0002] Aminoglycoside veterinary drugs play an important role in the prevention and control of animal diseases, but their residues in animal-derived foods may cause serious harm to human health, and thus become a public health issue of increasing concern. The Ministry of Agriculture of my country stipulates that the maximum residue limit (MRL) of streptomycin in milk is 200 μg / kg; Japan’s positive list system stipulates that the MRL of the total amount of streptomycin and dihydrostreptomycin in beef and pork is 0.6 mg / kg; The MRLs for streptomycin in bee products, milk, meat and liver are 0.2, 0.2, 0.5 and 0.5 mg / kg respectively; the U.S. Food and Drug Administration (FDA) stipulates that the MRL for streptomycin in milk is 0.125 mg / kg ; The World Health...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/72G01N2030/047G01N2030/062
Inventor 郭亚娟付晓燕杨军林陈江杨鸿泽
Owner 广电计量检测(西安)有限公司
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