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General use artificial antigen synthesis of aminoglycoside medicaments

An aminoglycoside, artificial antigen technology, applied in the field of biochemical industry, can solve the problems of improper use method, human health hazards, enhanced bacterial resistance, etc., and achieves the effect of simple synthesis steps and meeting the needs of research.

Inactive Publication Date: 2008-12-10
JIANGNAN UNIV
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  • Claims
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AI Technical Summary

Problems solved by technology

However, due to its improper use or non-compliance with the withdrawal period regulations, etc., it can cause its residues in animal products, which will bring serious harm to human health, such as destroying the balance of gastrointestinal flora and enhancing bacterial resistance, etc.
Existing microbial detection methods and instrumental detection methods are difficult to achieve rapid, accurate and multi-residue detection of aminoglycoside antibiotics
At present, there are no reports on ideal multi-residue immunoassay methods for aminoglycoside drugs at home and abroad

Method used

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  • General use artificial antigen synthesis of aminoglycoside medicaments
  • General use artificial antigen synthesis of aminoglycoside medicaments

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Experimental program
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Embodiment 1

[0015] (1) Preparation of artificial antigen

[0016] 15mg neomycin and 20mg bovine serum albumin BSA were dissolved in 10mL 0.1mol / L sodium carbonate buffer solution (pH7.3, containing 0.15mol / L NaCl), and the freshly prepared glutaraldehyde aqueous solution 1.0% with a mass concentration of 1% was added dropwise. mL, after stirring gently for 15 minutes, add sodium borohydride to the reaction solution to a final concentration of 10 mg / mL, and then incubate at 4°C for 1.5 hours to obtain the artificial antigen mixture.

[0017] Dialysis bag pre-treatment: Take a 10cm dialysis bag, boil it in boiling water for 5 minutes, rinse it with 60°C deionized water for 3 minutes, and store it in 4°C deionized water for later use.

[0018] The artificial antigen mixture was transferred into a dialysis bag, and dialyzed with 2×2L of 0.01M PBS solution and 2×2L of deionized water for 3 days. Finally, the liquid in the dialysis bag is made into powder by freeze-drying method to obtain the ...

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Abstract

The invention relates to a method for synthesizing general artificial antigen of aminoglycoside medicine, belonging to the biochemical engineering technical field. The method uses neamine as hapten, uses a glutaraldehyde method to couple the neamine with protein BSA as a carrier, and uses a gel electrophoresis method to measure the coupling ratio of coupled matter. The method successfully synthesizes the artificial antigen of neamine, is simple and effective in synthesis method, can be used in immune analysis completely, provides the necessary artificial antigen for after research of people, and can meet the needs of the research on the aminoglycoside medicine in China.

Description

technical field [0001] The invention discloses a method for synthesizing a general artificial antigen of aminoglycoside drugs, which belongs to the technical field of biochemical engineering. Background technique [0002] Neomycin, English name: Neamin, CAS No. 5144-52-5, molecular formula C 12 h 25 N 4 o 6 , commonly known as neomycin A, is a structural community of aminoglycoside drugs. Aminoglycosides are a class of antibiotics that combine amino sugar molecules with non-sugar glycogens. These antibiotics are widely used and are broad-spectrum antibiotics for therapeutic drug monitoring. Its function is characterized by the ability to bind to the ribosomal 30S subunit, causing it to synthesize abnormal proteins, hindering the release of easily synthesized proteins, thereby inhibiting the growth of bacteria. Clinically, aminoglycoside antibiotics are mainly used to treat intestinal infections caused by Escherichia coli, bacillary dysentery, skin and mucous membrane i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/10C07K14/765
Inventor 胥传来马伟彭池方徐丽广李灼坤
Owner JIANGNAN UNIV
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