74results about How to "The synthetic step works" patented technology

A preparation method of an artificial antigen of dexamethasone is provided, which belongs to the biochemistry filed. With dexamethasone as the raw material, the present invention utilizes the reaction between succinic anhydride and dexamethasone to produce dexamethasone succinate and obtain the artificial semi-antigen, then the active ester method is used to transform the semi-antigen into a intermediate body of active ester, which is combined with bovine serum to prepare the artificial antigen of dexamethasone, i.e. dexamethasone bovine serum protein. The present invention synthesizes the artificial antigen of dexamethasone antigen, and the obtained product can be used for researches on immunization method with glucocorticoid, has simplified and effective synthetic procedures, as well as feasibility to be applied to immune analysis, thereby providing a convenient path for the further research by the people, and being capable of satisfying the domestic needs on the study on the object.

A synthetic method for the general artificial antigen of a beta-lactam medicine belongs to the biochemical technical field. The invention adopts 6-amino penicillanic acid as hapten, couples the hapten with the carrier protein of bovine serum albumin BSA through an N-(m-maleimide group benzene methanoyl) succinimides MBS method, and determines the coupling ratio of the coupled matter through a gel electrophoresis method. The method successfully synthesizes the 6-APA general artificial antigen with a simple and effective synthesis process. The method which can completely be used for immune analysis provides necessary artificial antigen for future study and meets the requirements of domestic study on the artificial antigen.

A synthesis method of an universal artificial antigen of alkylphenol medicines belongs to the technical field of biochemical engineering. The synthesis method is as follows: nonyl phenol (NP) is used as hapten, and the active ester method is adopted to couple the hapten and the carrier protein bovine serum albumin (BSA) to obtain the universal artificial antigen of alkylphenol medicines. Polyacrylamide gel electrophoresis is adopted to detect the coupling ratio of the conjugate. By adopting the method, the universal artificial antigen of alkylphenol medicines can be synthesized successfully; the synthesis step is concise and effective; and the method can be completely used in immunoassay, provide the necessary artificial antigen for the future research of people and meet the need of the domestic research on the artificial antigen.

The invention relates to a method for synthesizing a genetic artificial antigen for perflurooctanoic acid medicines, which belongs to the biochemical technical field. The method takes perflurooctanoic acid PFOA as a hapten, couples the perflurooctanoic acid PFOA with a carrier protein, namely bovine serum albumin BSA by utilization of carbodiimide, and determines the coupling ratio of a coupling object by an ultraviolet scanner. The method successfully synthesizes the artificial antigen of the perflurooctanoic acid, namely perflurooctanoic acid-bovine serum albumin, has concise and effective synthetic procedures, can be completely used for immunoassay, provides a necessary artificial antigen for further research of people, and can meet the demand of domestic research of the artificial antigen.

The invention provides a synthesis method of 4-amino azobenzene artificial antigen, belonging to the technical field of biochemical engineering. The method comprises the following steps of: taking 4-amino azobenzene as hapten; coupling the 4-amino azobenzene with carrier protein BSA by means of diazotization; and measuring the coupling ratio of a coupling material by means of spectroscopic luminosity. The synthesis method successfully synthesizes the artificial antigen of the 4-amino azobenzene, is simple and effective in synthesis steps, can be completely used in immunoassay, provides the necessary artificial antigen to the later research of users, and can meet the requirement of the research of the 4-amino azobenzene in China.

The invention discloses a hybridoma cell strain capable of secreting an anti-pendimethalin monoclonal antibody and application of the hybridoma cell strain, and belongs to the field of food safety immunodetection. The invention provides the hybridoma cell strain capable of secreting the anti-pendimethalin monoclonal antibody, wherein the hybridoma cell strain is classified and named as a monoclonal cell strain, the preservation date is September 27, 2020, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.20791. The pendimethalin complete antigen and the same amount of Freund's adjuvant are mixed and emulsified, and BALB/c mice are immunized through neck and back subcutaneous multipoint injection. The hybridoma cell strain is prepared by the following steps of fusing splenocytes of high-titer and low-IC50 mice with mouse myeloma cells through a PEG (Polyethylene Glycol) method, and screening out hybrid cells after fusing the two cells by adopting a selective culture medium; and screening cells by an indirect competitive enzyme-linked immunosorbent assay and subcloning for three times to finally obtain the monoclonal antibody hybridoma cell strain ACE. The monoclonal antibody secreted by the hybridoma cell strain ACE has relatively high affinity and sensitivity to the pendimethalin, and a powerful detection method and means are provided for detection of the pendimethalin in animal-derived food.

A preparation method of an artificial antigen of diazepam is disclosed, which belongs to the biochemistry technology field. The first step of the present invention is preparation and detection of a semi-antigen, in which diazepam and succinic anhydride are used as the raw materials, and a carboxyl is inducted to the 5' benzene ring through Friedel-Crafts acylation, the second step is the preparation and detection of an artificial antigen, in which the product of step one is combined with bovine serum protein through mixed acid anhydride method to prepare the artificial antigen of diazepam, i.e. the diazepam-bovine serum protein. The present invention synthesizes the artificial antigen of diazepam, with simplified and effective synthetic procedures, as well as feasibility to be applied to immune analysis, thereby providing a convenient path for the further research by the people, and being capable of satisfying the domestic needs on the study on the object.

The invention discloses a method for synthesizing rhodamine B complete antigen, and belongs to the technical field of biochemical engineering. The method takes rhodamine B as a hapten, couples the rhodamine B with carrier protein BSA by a mixed acid anhydride method, and measures the coupling ratio of a conjugate by a spectrophotometry. The method successfully synthesizes the complete antigen of rhodamine B, has simple and effective synthesis steps, can be fully used in immunoassay, provides necessary artificial antigen for future research of people, and can meet the requirement of national rhodamine B research.

The invention relates to a method for preparing an artificial antigen of diethyl phthalate, belonging to the biochemical technical field. The method comprises the following steps that: 4-nitroisophthalic acid is taken as a raw material and generates esterification reaction with n-hexyl alcohol under the catalysis of concentrated sulfuric acid, and then a hapten is prepared by utilization of the ferrous powder reaction method to reduce nitryl on a benzene ring of diethyl phthalate into amido; and the artificial antigen of the diethyl phthalate, namely diethyl phthalate-bovine serum albumin, is prepared by utilization of the diazotization method to perform diazotization on the amido on the hapten and make the hapten combined with bovine serum albumin. The method synthesizes the artificial antigen of the diethyl phthalate, has concise and effective synthetic procedures, can be completely used for immunoassay, provides convenience for future research, and can meet the demand of domestic research of the artificial antigen.

The invention which relates to a preparation method of an alkylphenol medicine analogue NPA belongs to the technical field of the biochemical engineering. According to the preparation method, raw materials of azelaic acid and dimethyl azelate undergo a chemical reaction which comprises six steps to synthesize the alkylphenol medicine analogue NPA. The preparation method has the advantages of simple and effective synthetic step and very high purity of the obtained product, so a convenient approach is provided for the further research of people, and needs of the future immune research on alkylphenol medicines in China are satisfied.

The invention relates to a hybridoma cell line secreting an anti-chlorpromazine monoclonal antibody and an application thereof, which belong to the field of food safety immunoassay. The hybridoma cellline D secreting the anti-chlorpromazine monoclonal antibody prepared by the invention is preserved in the General Microbiology Center of the China Microbial Culture Collection Management Committee,and the preservation number is CGMCC No. 17304. The hybridoma cell line D has a good affinity and high sensitivity to chlorpromazine, the IC50 of the 50% inhibitory concentration of chlorpromazine is17.2 [mu]g/L, the hybridoma cell line can be used for the preparation of a chlorpromazine immunoassay kit and a colloidal gold test strip, which provides a powerful detection method and means for thedetection of chlorpromazine in animal-derived foods.

The invention relates to a synthesis method of a tetrabromobisphenol A analog-tetrabromobisphenol A hexanoic acid, belonging to the technical field of biochemical engineering. The method comprises the following steps: carrying out one-step chemical reaction on the raw material 6-bromohexanoic acid to obtain the tetrabromobisphenol A analog-tetrabromobisphenol A hexanoic acid, and carrying out liquid chromatography-mass spectrometry to identify and analyze the important intermediate product and end product. The method successfully synthesizes the tetrabromobisphenol A analog-tetrabromobisphenol A hexanoic acid by using safe and effective synthesis steps, provides favorable conditions for establishing the tetrabromobisphenol A enzyme-linked immunosorbent assay analysis process, and satisfies the national demands for the research.

The invention discloses a synthesis method of a eugenol artificial antigen, belonging to the technical field of biochemical industry. Eugenol reacts with tert-butyl 4-bromobutyrate to obtain a productwith a carboxyl group as a hapten, the hapten is coupled with carrier protein by a carbodiimide method, and characterized by an ultraviolet method, and a coupling ratio of a conjugate is determined.The eugenol artificial antigen is successfully synthesized with safe and effective synthesis steps, can be completely used for immunoassay, and provides the necessary artificial antigen for future research.

The invention relates to a method for synthetizing basic orange II artificial antigen and belongs to the technical field of biochemical industry. The method comprises the following steps of: preparing basic orange II-butyric acid artificial hapten from para-aminophenyl butyric acid and m-phenylenediamine serving as raw materials by a diazotization method; coupling the basic orange II-butyric acidartificial hapten with vector protein bovine serum albumin (BSA) by a carbodiimide method to obtain basic orange II-butyric acid-BSA conjugate; and measuring the coupling ratio of the conjugate by a spectrophotometry. A test result shows that the prepared basic orange II-butyric acid-BSA conjugate generates a good antibody for basic orange II, namely the basic orange II artificial antigen. The basic orange II artificial antigen is synthetized successfully by the method; the synthetic steps are simple and effective; the method can be completely applied to immunoassay; the necessary basic orange II artificial antigen is provided for people to do subsequent researches; and domestic requirements on the researches can be met.