74results about How to "The synthetic step works" patented technology

The invention relates to a method for synthesizing bisphenol A-type medicine general artificial antigen, belonging to the biochemical technical field. The method takes diphenolic acid BHPVA as a hapten, couples the diphenolic acid BHPVA and a carrier protein, namely bovine serum albumin BSA by utilization of the activated ester method, and then obtains the bisphenol A-type medicine general artificial antigen, namely diphenolic acid-bovine serum albumin; and the coupling ratio of an coupling object is determined by an ultraviolet scanner. The method successfully synthesizes the artificial antigen of the BHPVA, has concise and effective synthetic procedures, can be completely used for immunoassay, provides a necessary artificial antigen for further research of people, and can meet the demand of domestic research of the artificial antigen.

A preparation method of an artificial antigen of dexamethasone is provided, which belongs to the biochemistry filed. With dexamethasone as the raw material, the present invention utilizes the reaction between succinic anhydride and dexamethasone to produce dexamethasone succinate and obtain the artificial semi-antigen, then the active ester method is used to transform the semi-antigen into a intermediate body of active ester, which is combined with bovine serum to prepare the artificial antigen of dexamethasone, i.e. dexamethasone bovine serum protein. The present invention synthesizes the artificial antigen of dexamethasone antigen, and the obtained product can be used for researches on immunization method with glucocorticoid, has simplified and effective synthetic procedures, as well as feasibility to be applied to immune analysis, thereby providing a convenient path for the further research by the people, and being capable of satisfying the domestic needs on the study on the object.

The invention relates to a F-Ketamine hapten, a F-Ketamine antigen as well as a preparation method and application thereof. The structure of the F-Ketamine hapten is shown as a formula (I). The preparation method of the F-Ketamine hapten comprises the following steps: taking F-Ketamine and a compound shown as a formula (II) as raw materials, carrying out reacting, and introducing an active arm with carboxyl on carbonyl of the F-Ketamine to obtain the F-Ketamine hapten. Furthermore, after the F-Ketamine hapten is activated, the F-Ketamine hapten and carrier protein are subjected to a coupling reaction to obtain the F-Ketamine antigen. The invention initiates a novel F-Ketamine artificial hapten and a novel F-Ketamine artificial antigen for the first time; and the novel F-Ketamine artificialhapten and the novel F-Ketamine artificial antigen can be applied to an immunochromatography technology, have the advantages of high sensitivity, high specificity, simplicity, rapidness, easiness inoperation, no need of any large-scale instruments and equipment and the like, and can be used for large-scale preliminary screening of F-Ketamine.

A synthetic method for the general artificial antigen of a beta-lactam medicine belongs to the biochemical technical field. The invention adopts 6-amino penicillanic acid as hapten, couples the hapten with the carrier protein of bovine serum albumin BSA through an N-(m-maleimide group benzene methanoyl) succinimides MBS method, and determines the coupling ratio of the coupled matter through a gel electrophoresis method. The method successfully synthesizes the 6-APA general artificial antigen with a simple and effective synthesis process. The method which can completely be used for immune analysis provides necessary artificial antigen for future study and meets the requirements of domestic study on the artificial antigen.

A synthesis method of an universal artificial antigen of alkylphenol medicines belongs to the technical field of biochemical engineering. The synthesis method is as follows: nonyl phenol (NP) is used as hapten, and the active ester method is adopted to couple the hapten and the carrier protein bovine serum albumin (BSA) to obtain the universal artificial antigen of alkylphenol medicines. Polyacrylamide gel electrophoresis is adopted to detect the coupling ratio of the conjugate. By adopting the method, the universal artificial antigen of alkylphenol medicines can be synthesized successfully; the synthesis step is concise and effective; and the method can be completely used in immunoassay, provide the necessary artificial antigen for the future research of people and meet the need of the domestic research on the artificial antigen.

The invention relates to a preparation method of an artificial antigen applied to tartrazine, belonging to the technical field of biochemical engineering. According to the invention, a derivative TAR of the tartrazine is prepared by taking ethyl succinyl chloride and 2, 2-dimethyl-1, 2-dioxane-4,6-dione as raw materials through four-step chemical reaction, and the TAR which contains no sulfonic acid group is used as a hapten and coupled with the carrier protein BSA or OVA by adopting an active ester method to prepare the artificial antigen for the tartrazine; the coupling rate of the conjugate is measured by adopting the spectrophotometry; the specific antibody which corresponds to the tartrazine is generated in an immunizing manner; and the enzyme-linked immunodetection method is established. The method successfully synthesized the artificial antigen of the tartrazine which can be fully used for immunoassay with concise and effective steps, thus providing necessary artificial antigen of the tartrazine for the further research of people, and meeting the domestic demand for the research of the tartrazine.

The invention relates to a method for synthesizing a genetic artificial antigen for perflurooctanoic acid medicines, which belongs to the biochemical technical field. The method takes perflurooctanoic acid PFOA as a hapten, couples the perflurooctanoic acid PFOA with a carrier protein, namely bovine serum albumin BSA by utilization of carbodiimide, and determines the coupling ratio of a coupling object by an ultraviolet scanner. The method successfully synthesizes the artificial antigen of the perflurooctanoic acid, namely perflurooctanoic acid-bovine serum albumin, has concise and effective synthetic procedures, can be completely used for immunoassay, provides a necessary artificial antigen for further research of people, and can meet the demand of domestic research of the artificial antigen.

The invention relates to a method for synthesizing artificial antigen having aminoglycoside medicine multi-antigen determinant, belonging to the biochemical engineering technical field. The method uses neomycin, kanamycin and gentamicin as hapten, uses a carbodiimide method to couple the hapten with protein bovine serum protein BSA as a carrier, and uses a gel electrophoresis method to measure the coupling ratio of coupled matter. The method successfully synthesizes the artificial antigen having the aminoglycoside medicine multi-antigen determinant, is simple and effective in synthesis method, can be used in immune analysis completely, provides the necessary artificial antigen for after research of people, and can meet the needs of the research on the aminoglycoside medicine in China.

The invention provides a synthesis method of 4-amino azobenzene artificial antigen, belonging to the technical field of biochemical engineering. The method comprises the following steps of: taking 4-amino azobenzene as hapten; coupling the 4-amino azobenzene with carrier protein BSA by means of diazotization; and measuring the coupling ratio of a coupling material by means of spectroscopic luminosity. The synthesis method successfully synthesizes the artificial antigen of the 4-amino azobenzene, is simple and effective in synthesis steps, can be completely used in immunoassay, provides the necessary artificial antigen to the later research of users, and can meet the requirement of the research of the 4-amino azobenzene in China.

The invention provides a synthetic method of an artificial florfenicol amine antigen and belongs to the technical field of biochemical engineering. Florfenicol reacts with 4-benzyl bromide, 20% H2SO4, K2CO3 and NH3.CH3OH to obtain a product provided with a carboxy group, the product is hapten, then the hapten is coupled with carrier protein by using a carbodiimide method, and the coupling ratio of a conjugate is determined by using an ultraviolet method. The artificial florfenicol amine antigen is successively synthetized, synthesis steps are simple and effective, the artificial florfenicol amine antigen can be completedly used for immunoassay, and a necessary artificial antigen is provided for people for future research.

The invention discloses a hybridoma cell strain capable of secreting an anti-pendimethalin monoclonal antibody and application of the hybridoma cell strain, and belongs to the field of food safety immunodetection. The invention provides the hybridoma cell strain capable of secreting the anti-pendimethalin monoclonal antibody, wherein the hybridoma cell strain is classified and named as a monoclonal cell strain, the preservation date is September 27, 2020, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.20791. The pendimethalin complete antigen and the same amount of Freund's adjuvant are mixed and emulsified, and BALB / c mice are immunized through neck and back subcutaneous multipoint injection. The hybridoma cell strain is prepared by the following steps of fusing splenocytes of high-titer and low-IC50 mice with mouse myeloma cells through a PEG (Polyethylene Glycol) method, and screening out hybrid cells after fusing the two cells by adopting a selective culture medium; and screening cells by an indirect competitive enzyme-linked immunosorbent assay and subcloning for three times to finally obtain the monoclonal antibody hybridoma cell strain ACE. The monoclonal antibody secreted by the hybridoma cell strain ACE has relatively high affinity and sensitivity to the pendimethalin, and a powerful detection method and means are provided for detection of the pendimethalin in animal-derived food.

A preparation method of an artificial antigen of diazepam is disclosed, which belongs to the biochemistry technology field. The first step of the present invention is preparation and detection of a semi-antigen, in which diazepam and succinic anhydride are used as the raw materials, and a carboxyl is inducted to the 5' benzene ring through Friedel-Crafts acylation, the second step is the preparation and detection of an artificial antigen, in which the product of step one is combined with bovine serum protein through mixed acid anhydride method to prepare the artificial antigen of diazepam, i.e. the diazepam-bovine serum protein. The present invention synthesizes the artificial antigen of diazepam, with simplified and effective synthetic procedures, as well as feasibility to be applied to immune analysis, thereby providing a convenient path for the further research by the people, and being capable of satisfying the domestic needs on the study on the object.

The invention relates to a synthetic method of an artificial antigen suitable for alkylphenol medicaments. According to the technical scheme provided by the invention, nonyl phenol analogues are mainly used as haptens; the analogues are coupled with carrier protein bovine serum albumin (BSA) by an activated ester method; and the coupling ratio of the conjugate is determined by polyacrylamide gel electrophoresis. The synthetic method of the present invention can successfully synthesize an artificial antigen of nonyl phenol analogues; the synthetic steps are simple and effective, and can be used in immunoassays completely; the invention provides a convenient approach for future research, and can meet the requirements for domestic research.

The invention discloses a method for synthesizing rhodamine B complete antigen, and belongs to the technical field of biochemical engineering. The method takes rhodamine B as a hapten, couples the rhodamine B with carrier protein BSA by a mixed acid anhydride method, and measures the coupling ratio of a conjugate by a spectrophotometry. The method successfully synthesizes the complete antigen of rhodamine B, has simple and effective synthesis steps, can be fully used in immunoassay, provides necessary artificial antigen for future research of people, and can meet the requirement of national rhodamine B research.

The invention relates to a method for preparing an artificial antigen of diethyl phthalate, belonging to the biochemical technical field. The method comprises the following steps that: 4-nitroisophthalic acid is taken as a raw material and generates esterification reaction with n-hexyl alcohol under the catalysis of concentrated sulfuric acid, and then a hapten is prepared by utilization of the ferrous powder reaction method to reduce nitryl on a benzene ring of diethyl phthalate into amido; and the artificial antigen of the diethyl phthalate, namely diethyl phthalate-bovine serum albumin, is prepared by utilization of the diazotization method to perform diazotization on the amido on the hapten and make the hapten combined with bovine serum albumin. The method synthesizes the artificial antigen of the diethyl phthalate, has concise and effective synthetic procedures, can be completely used for immunoassay, provides convenience for future research, and can meet the demand of domestic research of the artificial antigen.

The invention which relates to a preparation method of an alkylphenol medicine analogue NPA belongs to the technical field of the biochemical engineering. According to the preparation method, raw materials of azelaic acid and dimethyl azelate undergo a chemical reaction which comprises six steps to synthesize the alkylphenol medicine analogue NPA. The preparation method has the advantages of simple and effective synthetic step and very high purity of the obtained product, so a convenient approach is provided for the further research of people, and needs of the future immune research on alkylphenol medicines in China are satisfied.

The invention relates to a hybridoma cell line secreting an anti-chlorpromazine monoclonal antibody and an application thereof, which belong to the field of food safety immunoassay. The hybridoma cellline D secreting the anti-chlorpromazine monoclonal antibody prepared by the invention is preserved in the General Microbiology Center of the China Microbial Culture Collection Management Committee,and the preservation number is CGMCC No. 17304. The hybridoma cell line D has a good affinity and high sensitivity to chlorpromazine, the IC50 of the 50% inhibitory concentration of chlorpromazine is17.2 [mu]g / L, the hybridoma cell line can be used for the preparation of a chlorpromazine immunoassay kit and a colloidal gold test strip, which provides a powerful detection method and means for thedetection of chlorpromazine in animal-derived foods.

The invention relates to a preparation method of an artificial antigen of 4-aminobenzophenone, belonging to the technical field of biochemical industry. The 4-aminobenzophenone is adopted as a half antigen because benzophenone has no radical necessary for coupling but has the same matrix as the 4-aminobenzophenone. In the invention, the artificial antigen of 4-aminobenzophenone is prepared by utilizing an analog of benzophenone, the 4-aminobenzophenone is a half antigen and is coupled with a carrier BSA (Bovine Serum Albumin) by a diazotization method, and the coupling ratio of the coupling substances is measured by a spectrophotometry. The artificial antigen of 4-aminobenzophenone is successfully synthesized by using the invention, and the synthesis steps are simple and effective. The invention can be fully applied to immunoassay, provide necessary artificial antigens for later research of people and satisfy the national needs for researching the benzophenone and the 4-aminobenzophenone.

The invention discloses a preparation method for artificial antigen of erythrosin pigment and belongs to the technical field of biochemical engineering. Erythrosin derivant ETS is used as a hapten and is conjugated with a carrier protein-BSA (Bovine Serum Albumin) through an active ester method to obtain the artificial antigen of erythrosine which is erythrosin derivant ETS-BSA; and the conjugating ratio of a conjugate is determined through an ultraviolet scanner. The preparation method for artificial antigen of erythrosin pigment can be used for successfully synthesizing the antigen of erythrosin derivant ETS, has simple and effective synthesis steps, can be completely used in immunoassay, provides necessary artificial antigen for further study of people and can satisfy the domestic study demands on the artificial antigen.

The invention relates to a synthesis method of a tetrabromobisphenol A analog-tetrabromobisphenol A hexanoic acid, belonging to the technical field of biochemical engineering. The method comprises the following steps: carrying out one-step chemical reaction on the raw material 6-bromohexanoic acid to obtain the tetrabromobisphenol A analog-tetrabromobisphenol A hexanoic acid, and carrying out liquid chromatography-mass spectrometry to identify and analyze the important intermediate product and end product. The method successfully synthesizes the tetrabromobisphenol A analog-tetrabromobisphenol A hexanoic acid by using safe and effective synthesis steps, provides favorable conditions for establishing the tetrabromobisphenol A enzyme-linked immunosorbent assay analysis process, and satisfies the national demands for the research.

The invention relates to a synthetic method of quinolones antibiotic antigen, which belongs to the biological chemistry technical field. The raw material of the invention is the norfloxacin, the hapten N-2-ethylamine-norfloxacin is produced through the derivatization of norfloxacin and the protection of carboxyl, and the quinolones antibiotic multi-cluster antigen N-2-ethylamine-norfloxacin-bovine serum albumin is compounded by the coupling of the albumin and the hapten N-2-ethylamine-norfloxacin. The invention successfully compounds the quinolones antibiotic multi-cluster antigen, and the combining procedure is simple; and the purity quotient of the quinolones antibiotic antigen can reach more than 99 percent after the purification. The invention can be used for the application of quinolones multi-cluster EIA kits, and the invention provides a convenient way for a further research, and the need of the research of the invention at home can be satisfied.

A preparation method of an artificial antigen of dexamethasone is provided, which belongs to the biochemistry filed. With dexamethasone as the raw material, the present invention utilizes the reaction between succinic anhydride and dexamethasone to produce dexamethasone succinate and obtain the artificial semi-antigen, then the active ester method is used to transform the semi-antigen into a intermediate body of active ester, which is combined with bovine serum to prepare the artificial antigen of dexamethasone, i.e. dexamethasone bovine serum protein. The present invention synthesizes the artificial antigen of dexamethasone antigen, and the obtained product can be used for researches on immunization method with glucocorticoid, has simplified and effective synthetic procedures, as well asfeasibility to be applied to immune analysis, thereby providing a convenient path for the further research by the people, and being capable of satisfying the domestic needs on the study on the object.

The invention provides an estriol-specificity-resistant monoclonal antibody hybridoma cell strain NaN-2 and application thereof and belongs to the technical field of food safety immune detection. The estriol-specificity-resistant monoclonal antibody hybridoma cell strain NaN-2 is preserved in General Microbiology Center, Microbial Preservation Management Committee, China, and the preservation number is CGMCC No. 12016. An estriol-specificity-resistant monoclonal antibody is generated by secretion of the hybridoma cell strain NaN-2. The monoclonal antibody secreted by the cell strain has good specificity to estriol (the IC 50 value is 0.25 ng / mL) and can be used for estriol specificity detection in food safety. The obtained estriol-resistant monoclonal antibody cell strain has good detection sensitivity and appetency to estriol, a novel method for synthesizing estriol immunogen is further provided, the synthesis step of hapten is more simplified and effective, and a thought and method for synthesizing immunogen are provided for people to conduct research in the future.

The invention discloses a synthesis method of a eugenol artificial antigen, belonging to the technical field of biochemical industry. Eugenol reacts with tert-butyl 4-bromobutyrate to obtain a productwith a carboxyl group as a hapten, the hapten is coupled with carrier protein by a carbodiimide method, and characterized by an ultraviolet method, and a coupling ratio of a conjugate is determined.The eugenol artificial antigen is successfully synthesized with safe and effective synthesis steps, can be completely used for immunoassay, and provides the necessary artificial antigen for future research.

A synthetic method of common artificial antigen of beta-lactam drug belongs to the biochemical technical field, wherein, the invention takes 7-aminocephalosporanic acid as hapten which is in coupling with carrier protein BSA through glutaraldehyde method; the coupling ratio of conjugates is measured by an UV scanner. The invention realizes successful synthesis of artificial antigen of 7-aminocephalosporanic acid along with simple and effective synthesis procedure; therefore, the invention which can be used in immunity analysis provides necessary artificial antigen for future research and meets the domestic requirements of the research on the artificial antigen.

The invention discloses a hybridoma cell line YY capable of secreting an anti-sildenafil monoclonal antibody and its application and belongs to the technical field of food safety immunoassay. The hybridoma cell line YY is preserved in the China general microbiological culture collection center (CGMCC) and has an accession number CGMCC No. 13100. The hybridoma cell line YY can secrete a monoclonal antibody having good affinity and high sensitivity to sildenafil and 50% inhibition concentration IC50 to sildenafil of 0.48 micrograms per milliliter. The hybridoma cell line YY can be used for preparation of a sildenafil immunoassay kit and a colloidal gold test strip and provides a good detection method for sildenafil in Chinese patent medicines and health products.

The invention relates to a hybridoma cell strain capable of secreting anti-dinitolmide monoclonal antibody and an application of the hybridoma cell strain, and belongs to the field of food safety immunodetection. The hybridoma cell strain DAS3H10 capable of secreting the anti-dinitolmide monoclonal antibody is preserved in China General Microbiological Culture Collection Center (CGMCC), the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the strain is classified and named as a monoclonal cell strain, the preservation date is November 28, 2019, and the preservation number is CGMCC No. 19165. A monoclonal antibody prepared from the hybridoma cell strain DAS3H10 has relatively high affinity and high sensitivity todinitolmide, has 50% inhibitory concentration IC50 on dinitolmide of being 9.01 ng / mL, and can be used for preparing an immunodetection test kit and a colloidal gold test strip of dinitolmide. A powerful detection method and means are provided for detection of dinitolmide in animal-derived food.

The invention relates to a method for synthetizing basic orange II artificial antigen and belongs to the technical field of biochemical industry. The method comprises the following steps of: preparing basic orange II-butyric acid artificial hapten from para-aminophenyl butyric acid and m-phenylenediamine serving as raw materials by a diazotization method; coupling the basic orange II-butyric acidartificial hapten with vector protein bovine serum albumin (BSA) by a carbodiimide method to obtain basic orange II-butyric acid-BSA conjugate; and measuring the coupling ratio of the conjugate by a spectrophotometry. A test result shows that the prepared basic orange II-butyric acid-BSA conjugate generates a good antibody for basic orange II, namely the basic orange II artificial antigen. The basic orange II artificial antigen is synthetized successfully by the method; the synthetic steps are simple and effective; the method can be completely applied to immunoassay; the necessary basic orange II artificial antigen is provided for people to do subsequent researches; and domestic requirements on the researches can be met.

The present invention relates to an oxazepam hapten, an oxazepam antigen, a preparation method and applications thereof, the structure of the oxazepam hapten is represented by a formula (I), the preparation method of the oxazepam hapten comprises that oxazepam and dianhydride are adopted as raw materials, a Friedel-Crafts acylation reaction is adopted, and carboxyl is introduced to the 5-positionbenzene ring of oxazepam, and the oxazepam hapten is obtained. The preparation method of the oxazepam antigen comprises the following steps: activating the oxazepam hapten, and carrying out a couplingreaction on the oxazepam hapten and carrier protein to obtain the oxazepam antigen. The invention initiates the preparation method of the oxazepam artificial hapten and the oxazepam artificial antigen for the first time, the synthesis steps are simple and effective, the oxazepam artificial hapten and the oxazepam artificial antigen can be completely used in immunoassay, and a convenient way is provided for people to detect and research oxazepam in the future.