Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Synthesis method for generally used artificial antigen of Beta-lactam medicine

A technology of artificial antigen and hapten, which is applied in the preparation method of peptide, chemical instrument and method, biological test, etc., to achieve the effect of simple synthesis steps and meeting the needs of research.

Inactive Publication Date: 2008-04-02
JIANGNAN UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the colloidal gold immunoassay method for cephalosporin antibiotics in China. In order to make up for this gap, a cephalosporin antibiotic intermediate 7-aminocephalosporanic acid was designed and synthesized for cephalosporin antibiotics. complete antigen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Synthesis method for generally used artificial antigen of Beta-lactam medicine
  • Synthesis method for generally used artificial antigen of Beta-lactam medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Preparation of artificial antigen

[0019] Weigh 27.2 mg (0.1 mmol) of 7-aminocephalosporanic acid and dissolve it in 8 mL of PBS (0.01 mol / L) solution. After fully dissolving, add 40 μL of 25% glutaraldehyde (0.01 mmol) dropwise and react for 30 min. Another 68 mg (0.001 mmol) of BSA was weighed and dissolved in 2 mL of PBS (0.01 mol / L) solution, then added dropwise to the above reaction solution, and stirred at room temperature for 3 h. That is, the artificial antigen mixture was obtained.

[0020] Dialysis bag pre-treatment: Take a 10cm dialysis bag, boil it in boiling water for 5 minutes, rinse it with 60°C deionized water for 3 minutes, and store it in 4°C deionized water for later use.

[0021] The artificial antigen mixture was transferred into a dialysis bag, and dialyzed with 2×2L of 0.01M pH7.4 PBS buffer and 2×2L of deionized water for 3 days. Finally, the liquid in the dialysis bag is made into powder by freeze-drying method to obtain the artificial an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A synthetic method of common artificial antigen of beta-lactam drug belongs to the biochemical technical field, wherein, the invention takes 7-aminocephalosporanic acid as hapten which is in coupling with carrier protein BSA through glutaraldehyde method; the coupling ratio of conjugates is measured by an UV scanner. The invention realizes successful synthesis of artificial antigen of 7-aminocephalosporanic acid along with simple and effective synthesis procedure; therefore, the invention which can be used in immunity analysis provides necessary artificial antigen for future research and meets the domestic requirements of the research on the artificial antigen.

Description

technical field [0001] The invention relates to a method for synthesizing a general artificial antigen of β-lactam drugs, which belongs to the field of biochemical technology. Background technique [0002] 7-Aminocephalosporanic acid, English name: 7-Amino Cephalosporanic Acid (7-ACA), CAS No. 957-68-6, molecular formula C 10 h 12 N 2 o 5 S, is an intermediate of cephalosporin antibiotics in β-lactams. β-lactam antibiotics refer to a class of antibiotics containing a β-lactam ring in their chemical structure, mainly including penicillins and cephalosporins, and their function is characterized by being able to inhibit the activity of bacterial mucopeptide transpeptidase, thereby preventing bacterial Synthesis of cell walls, exhibits bactericidal activity. In animal husbandry, β-lactam antibiotics are widely used to control mastitis in dairy cows, and treat animal urinary tract, gastrointestinal tract and respiratory tract infections. However, due to its improper use or ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53C07K14/765C07K1/10
Inventor 胥传来谢会玲彭池方刘利强胡拥明
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com