Synthesis method for generally used artificial antigen of Beta-lactam medicine

A technology of artificial antigen and hapten, which is applied in the preparation method of peptide, chemical instrument and method, biological test, etc., to achieve the effect of simple synthesis steps and meeting the needs of research.

Inactive Publication Date: 2008-04-02
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the colloidal gold immunoassay method for cephalosporin antibiotics in China. In order to make up for this gap, a cephalosporin antibiotic intermediate 7-aminocephalosporanic acid was designed and synthesized for cephalosporin antibiotics. complete antigen

Method used

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  • Synthesis method for generally used artificial antigen of Beta-lactam medicine
  • Synthesis method for generally used artificial antigen of Beta-lactam medicine

Examples

Experimental program
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Embodiment 1

[0018] (1) Preparation of artificial antigen

[0019] Weigh 27.2 mg (0.1 mmol) of 7-aminocephalosporanic acid and dissolve it in 8 mL of PBS (0.01 mol / L) solution. After fully dissolving, add 40 μL of 25% glutaraldehyde (0.01 mmol) dropwise and react for 30 min. Another 68 mg (0.001 mmol) of BSA was weighed and dissolved in 2 mL of PBS (0.01 mol / L) solution, then added dropwise to the above reaction solution, and stirred at room temperature for 3 h. That is, the artificial antigen mixture was obtained.

[0020] Dialysis bag pre-treatment: Take a 10cm dialysis bag, boil it in boiling water for 5 minutes, rinse it with 60°C deionized water for 3 minutes, and store it in 4°C deionized water for later use.

[0021] The artificial antigen mixture was transferred into a dialysis bag, and dialyzed with 2×2L of 0.01M pH7.4 PBS buffer and 2×2L of deionized water for 3 days. Finally, the liquid in the dialysis bag is made into powder by freeze-drying method to obtain the artificial an...

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Abstract

A synthetic method of common artificial antigen of beta-lactam drug belongs to the biochemical technical field, wherein, the invention takes 7-aminocephalosporanic acid as hapten which is in coupling with carrier protein BSA through glutaraldehyde method; the coupling ratio of conjugates is measured by an UV scanner. The invention realizes successful synthesis of artificial antigen of 7-aminocephalosporanic acid along with simple and effective synthesis procedure; therefore, the invention which can be used in immunity analysis provides necessary artificial antigen for future research and meets the domestic requirements of the research on the artificial antigen.

Description

technical field [0001] The invention relates to a method for synthesizing a general artificial antigen of β-lactam drugs, which belongs to the field of biochemical technology. Background technique [0002] 7-Aminocephalosporanic acid, English name: 7-Amino Cephalosporanic Acid (7-ACA), CAS No. 957-68-6, molecular formula C 10 h 12 N 2 o 5 S, is an intermediate of cephalosporin antibiotics in β-lactams. β-lactam antibiotics refer to a class of antibiotics containing a β-lactam ring in their chemical structure, mainly including penicillins and cephalosporins, and their function is characterized by being able to inhibit the activity of bacterial mucopeptide transpeptidase, thereby preventing bacterial Synthesis of cell walls, exhibits bactericidal activity. In animal husbandry, β-lactam antibiotics are widely used to control mastitis in dairy cows, and treat animal urinary tract, gastrointestinal tract and respiratory tract infections. However, due to its improper use or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53C07K14/765C07K1/10
Inventor 胥传来谢会玲彭池方刘利强胡拥明
Owner JIANGNAN UNIV
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