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Synthesis of multi-antigenic determinant artificial antigen with aminoglycoside medicaments

An aminoglycoside and artificial antigen technology, applied in the field of biochemical industry, can solve the problems of backward detection technology, multi-residue, restriction of difficult aminoglycoside antibiotics, etc., and achieve the effect of simple synthesis steps and meeting the needs of research.

Inactive Publication Date: 2008-12-10
JIANGNAN UNIV
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  • Abstract
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Problems solved by technology

However, the detection technology of this type of drug is still relatively backward, which has become the main factor restricting the supervision of this type of drug by the government. The existing microbial detection method and instrument detection method are difficult to achieve rapid, accurate and multi-residue detection of aminoglycoside antibiotics
At present, there are no reports on ideal multi-residue immunoassay methods for aminoglycoside drugs at home and abroad

Method used

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  • Synthesis of multi-antigenic determinant artificial antigen with aminoglycoside medicaments
  • Synthesis of multi-antigenic determinant artificial antigen with aminoglycoside medicaments

Examples

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Embodiment 1

[0027] (1) Conjugate (NM) L - Preparation of BSA

[0028] Dissolve 25mg of neomycin (NM) and 40mg of bovine serum albumin BSA in 3mL of 0.01mol / L PBS buffer, add dropwise the methanol solution of water-soluble carbodiimide (75μL EDC dissolved in 75μL methanol), gently Stir for 3 hours. The ready-to-get conjugate (NM) L -BSA mixture.

[0029] Dialysis bag pre-treatment: Take a 10cm dialysis bag, boil it in boiling water for 5 minutes, rinse it with 60°C deionized water for 3 minutes, and store it in 4°C deionized water for later use.

[0030] The reaction solution was put into a dialysis bag and dialyzed in 0.01mol / L PBS for 3 days, and the solution was changed three times a day. The dialysis bag was placed in polyethylene glycol 20000, and the reaction solution was concentrated to 3 mL for use.

[0031] (2) Conjugates (NM) L - Preparation of (KM)m-BSA

[0032] Add Kanamycin (KM) 35mg to (NM) L - To the concentrated solution of BSA in PBS, add the freshly prepared methan...

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Abstract

The invention relates to a method for synthesizing artificial antigen having aminoglycoside medicine multi-antigen determinant, belonging to the biochemical engineering technical field. The method uses neomycin, kanamycin and gentamicin as hapten, uses a carbodiimide method to couple the hapten with protein bovine serum protein BSA as a carrier, and uses a gel electrophoresis method to measure the coupling ratio of coupled matter. The method successfully synthesizes the artificial antigen having the aminoglycoside medicine multi-antigen determinant, is simple and effective in synthesis method, can be used in immune analysis completely, provides the necessary artificial antigen for after research of people, and can meet the needs of the research on the aminoglycoside medicine in China.

Description

technical field [0001] The invention relates to a method for synthesizing an artificial antigen with multiple antigenic determinants of aminoglycoside drugs, which belongs to the field of biochemical technology. Background technique [0002] Aminoglycosides are a class of antibiotics composed of amino sugar molecules and glycogens in non-sugar parts, and are broad-spectrum antibacterial drugs. Clinically, aminoglycoside antibiotics are mainly used to treat intestinal infections caused by Escherichia coli, bacillary dysentery, skin and mucous membrane infections, and eye infections, but they have severe ototoxicity and nephrotoxicity. Such drugs are difficult to degrade in the environment and can be passed to humans through the food chain. The long-term use of large amounts of aminoglycosides in animal diets will cause serious pollution to the environment. In order to ensure the safety of animal food, the addition of such drugs in feed should be strictly supervised and manag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/10C07K14/765
Inventor 胥传来马伟彭池方徐丽广李灼坤
Owner JIANGNAN UNIV
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