Synthetic method of bisphenol A medicament universal artificial antigen

A technology of artificial antigen and synthesis method, which is applied in the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., to achieve the effect of concise synthesis steps and meeting the needs of research.

Inactive Publication Date: 2008-12-24
JIANGNAN UNIV
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Problems solved by technology

So far, there is no better report on the immunological detection method for the multi-residue of bisphenol structure in China. Therefore, a complete detection method for the structure detection of bisphenol A was designed and synthesized using bisphenol acid, which is an analogue of bisphenol A, as a hapten. antigen

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  • Synthetic method of bisphenol A medicament universal artificial antigen

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Embodiment 1

[0017] (1) Preparation of artificial antigen

[0018] 7.2 mg (0.025 mmol) of bisphenolic acid was dissolved in 1 mL of dimethyl sulfoxide (DMSO) solution, and after fully dissolved, 6.7 μL (0.029 mmol) of carbodiimide (EDC) and 3.5 mg (0.029 mmol) of N- Hydroxysuccinimide (NHS) was reacted with stirring at room temperature for 3 hours, and it was liquid A; 45mg (0.00068mmol) bovine serum albumin was dissolved in 4mL NaHCO 3 (pH 7.0, 0.1mol / L) solution, then add the above solution A dropwise, stir and react at room temperature for 3 hours, and obtain the artificial antigen mixture.

[0019] Dialysis bag pre-treatment: Take a 10cm dialysis bag, boil it in boiling water for 5 minutes, rinse it with 60°C deionized water for 3 minutes, and store it in 4°C deionized water for later use.

[0020] The artificial antigen mixture was transferred into a dialysis bag, and dialyzed with 2×2L of 0.01M PBS solution and 2×2L of deionized water for 3 days. Finally, the liquid in the dialysis...

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Abstract

The invention relates to a method for synthesizing bisphenol A-type medicine general artificial antigen, belonging to the biochemical technical field. The method takes diphenolic acid BHPVA as a hapten, couples the diphenolic acid BHPVA and a carrier protein, namely bovine serum albumin BSA by utilization of the activated ester method, and then obtains the bisphenol A-type medicine general artificial antigen, namely diphenolic acid-bovine serum albumin; and the coupling ratio of an coupling object is determined by an ultraviolet scanner. The method successfully synthesizes the artificial antigen of the BHPVA, has concise and effective synthetic procedures, can be completely used for immunoassay, provides a necessary artificial antigen for further research of people, and can meet the demand of domestic research of the artificial antigen.

Description

technical field [0001] The invention discloses a method for synthesizing a general artificial antigen of bisphenol A drugs, which belongs to the technical field of biochemical engineering. Background technique [0002] Bisphenolic acid, English name: 4,4-Bis (4-hydroxyphenyl) valeric acid (BHPVA), CAS No. 126-00-1, molecular formula C 17 h 18 o 4 , is an analogue of bisphenol A. Bisphenol A (Bisphenol A, BPA) is a kind of "Endocrine Disrupting Chemicals (ECD)" newly discovered from food packaging materials in recent years, which has certain estrogenic properties. The discovery of BPA has aroused widespread concern for food and packaging materials in various countries. There have been reports of detection of BPA from canned vegetables, baby preparations, and food cans. BPA is the main raw material for the production of PC resin and epoxy (EP) resin, and is also used in phenolic resin, plastic polyester, antioxidant and polyvinyl chloride (PVC) stabilizer. Therefore, it h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K1/14C07K1/10
Inventor 胥传来李灼坤彭池方马伟徐丽广袁媛谢会玲
Owner JIANGNAN UNIV
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